CA3211849A1 - Methodes et dispositifs permettant le developpement embryonnaire de souris ex-utero - Google Patents

Methodes et dispositifs permettant le developpement embryonnaire de souris ex-utero Download PDF

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Publication number
CA3211849A1
CA3211849A1 CA3211849A CA3211849A CA3211849A1 CA 3211849 A1 CA3211849 A1 CA 3211849A1 CA 3211849 A CA3211849 A CA 3211849A CA 3211849 A CA3211849 A CA 3211849A CA 3211849 A1 CA3211849 A1 CA 3211849A1
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embryo
culturing
stage
serum
medium
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Yaqub Hanna
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Yeda Research and Development Co Ltd
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Yeda Research and Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0604Whole embryos; Culture medium therefor
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/36Means for collection or storage of gas; Gas holders
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    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control
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    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/10Rotating vessel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14Incubators; Climatic chambers
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00Specific components of cell culture medium
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/34Sugars
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2521/00Culture process characterised by the use of hydrostatic pressure, flow or shear forces

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Computer Hardware Design (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

L'invention concerne des méthodes et des dispositifs permettant le développement embryonnaire de souris ex-utéro. Par conséquent, l'invention concerne une méthode de culture ex vivo d'un embryon de souris à un stade zygote dans des conditions qui permettent des développements de l'embryon à l'organogenèse ou tout stade de développement entre ceux-ci. L'invention concerne également un système d'incubation f?tal et des méthodes d'utilisation de celui-ci.
CA3211849A 2021-03-16 2022-03-15 Methodes et dispositifs permettant le developpement embryonnaire de souris ex-utero Pending CA3211849A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IL281561 2021-03-16
IL281561A IL281561A (en) 2021-03-16 2021-03-16 Methods and devices for growing mouse embryos outside the uterus
PCT/IL2022/050294 WO2022195589A2 (fr) 2021-03-16 2022-03-15 Méthodes et dispositifs permettant le développement embryonnaire de souris ex-utéro

Publications (1)

Publication Number Publication Date
CA3211849A1 true CA3211849A1 (fr) 2022-09-22

Family

ID=80978878

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3211849A Pending CA3211849A1 (fr) 2021-03-16 2022-03-15 Methodes et dispositifs permettant le developpement embryonnaire de souris ex-utero

Country Status (6)

Country Link
US (1) US20240026262A1 (fr)
EP (1) EP4308687A2 (fr)
CN (1) CN117441009A (fr)
CA (1) CA3211849A1 (fr)
IL (2) IL281561A (fr)
WO (1) WO2022195589A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118006464B (zh) * 2024-04-10 2024-06-21 江苏立维斯德生物技术有限公司 一种轻便式细胞低氧培养装置

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Also Published As

Publication number Publication date
EP4308687A2 (fr) 2024-01-24
CN117441009A (zh) 2024-01-23
WO2022195589A3 (fr) 2022-12-01
IL305970A (en) 2023-11-01
US20240026262A1 (en) 2024-01-25
WO2022195589A2 (fr) 2022-09-22
IL281561A (en) 2022-10-01

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