CA3176759A1 - Procede et marqueurs pour l'identification et la quantification relative d'une sequence d'acide nucleique, d'une mutation, d'un nombre de copies ou de changements de methylation a l'aide de combinaisons de nuclease, de ligature, de reparation d'adn et de reactions par polymerase avec prevention de remanenc - Google Patents

Procede et marqueurs pour l'identification et la quantification relative d'une sequence d'acide nucleique, d'une mutation, d'un nombre de copies ou de changements de methylation a l'aide de combinaisons de nuclease, de ligature, de reparation d'adn et de reactions par polymerase avec prevention de remanenc Download PDF

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Publication number
CA3176759A1
CA3176759A1 CA3176759A CA3176759A CA3176759A1 CA 3176759 A1 CA3176759 A1 CA 3176759A1 CA 3176759 A CA3176759 A CA 3176759A CA 3176759 A CA3176759 A CA 3176759A CA 3176759 A1 CA3176759 A1 CA 3176759A1
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Canada
Prior art keywords
markers
dna
cancer
specific
adenocarcinoma
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Pending
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CA3176759A
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English (en)
Inventor
Francis Barany
Manny D. BACOLOD
Jianmin Huang
Philip B. Feinberg
Aashiq H. MIRZA
Sarah F. Giardina
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Cornell University
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Individual
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Publication of CA3176759A1 publication Critical patent/CA3176759A1/fr
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/331—Methylation site specific nuclease
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/501—Ligase
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
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    • C12Q2521/514—Mismatch repair protein
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/539—Deaminase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/121—Modifications characterised by incorporating both deoxyribonucleotides and ribonucleotides
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
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    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C12Q2533/107—Probe or oligonucleotide ligation
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des procédés pour identifier et/ou quantifier une faible abondance, des mutations de bases nucléotidiques, des insertions, des délétions, des translocations, des variants d'épissage, des variants d'ARNmi, des produits de transcription alternatifs, des sites initiateurs alternatifs, des séquences codantes alternatives, des séquences non codantes alternatives, des épissages alternatifs, des insertions d'exons, des délétions d'exons, des insertions d'introns, ou un autre réarrangement au niveau du génome et/ou des bases nucléotidiques méthylées ou hydroxyméthylées, ainsi que des marqueurs pour identifier un cancer précoce, surveiller le traitement d'un cancer, et identifier la récidive précoce d'un cancer.
CA3176759A 2020-05-01 2021-04-29 Procede et marqueurs pour l'identification et la quantification relative d'une sequence d'acide nucleique, d'une mutation, d'un nombre de copies ou de changements de methylation a l'aide de combinaisons de nuclease, de ligature, de reparation d'adn et de reactions par polymerase avec prevention de remanenc Pending CA3176759A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063019142P 2020-05-01 2020-05-01
US63/019,142 2020-05-01
PCT/US2021/029998 WO2021222647A2 (fr) 2020-05-01 2021-04-29 Procédé et marqueurs pour l'identification et la quantification relative d'une séquence d'acide nucléique, d'une mutation, d'un nombre de copies ou de changements de méthylation à l'aide de combinaisons de nucléase, de ligature, de réparation d'adn et de réactions par polymérase avec prévention de rémanence

Publications (1)

Publication Number Publication Date
CA3176759A1 true CA3176759A1 (fr) 2021-11-04

Family

ID=78332228

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3176759A Pending CA3176759A1 (fr) 2020-05-01 2021-04-29 Procede et marqueurs pour l'identification et la quantification relative d'une sequence d'acide nucleique, d'une mutation, d'un nombre de copies ou de changements de methylation a l'aide de combinaisons de nuclease, de ligature, de reparation d'adn et de reactions par polymerase avec prevention de remanenc

Country Status (8)

Country Link
US (1) US20230287484A1 (fr)
EP (1) EP4143313A2 (fr)
JP (1) JP2023524067A (fr)
AU (1) AU2021263936A1 (fr)
BR (1) BR112022022179A2 (fr)
CA (1) CA3176759A1 (fr)
GB (1) GB2611639A (fr)
WO (1) WO2021222647A2 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114196740A (zh) * 2021-12-09 2022-03-18 南京普济生物有限公司 用于同时识别多种基因类型的数字扩增检测方法、检测产品和检测试剂盒
JP2024043823A (ja) * 2022-09-20 2024-04-02 株式会社東芝 対象のがん罹患の可能性を分析する方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7906288B2 (en) * 2006-01-04 2011-03-15 The Johns Hopkins University Compare-MS: method rapid, sensitive and accurate detection of DNA methylation
EP2825645B1 (fr) * 2012-03-15 2016-10-12 New England Biolabs, Inc. Procédés et compositions permettant de distinguer la cytosine de ses variantes et d'analyser le méthylome
WO2016057832A2 (fr) * 2014-10-08 2016-04-14 Cornell University Procédé pour l'identification et la détermination quantitative relative de l'expression de séquences d'acide nucléique, variant d'épissage, translocation, nombre de copies ou modifications de méthylation à l'aide de nucléases combinées, ligature, et réactions par polymérase avec prévention de l'entraînement

Also Published As

Publication number Publication date
WO2021222647A3 (fr) 2021-12-30
BR112022022179A2 (pt) 2022-12-13
JP2023524067A (ja) 2023-06-08
GB2611639A (en) 2023-04-12
GB202217105D0 (en) 2022-12-28
WO2021222647A2 (fr) 2021-11-04
US20230287484A1 (en) 2023-09-14
AU2021263936A1 (en) 2022-12-15
EP4143313A2 (fr) 2023-03-08

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