CA3164387A1 - Process for the production of closed linear dna - Google Patents
Process for the production of closed linear dna Download PDFInfo
- Publication number
- CA3164387A1 CA3164387A1 CA3164387A CA3164387A CA3164387A1 CA 3164387 A1 CA3164387 A1 CA 3164387A1 CA 3164387 A CA3164387 A CA 3164387A CA 3164387 A CA3164387 A CA 3164387A CA 3164387 A1 CA3164387 A1 CA 3164387A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- interest
- sequence
- template
- closed linear
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/713—Double-stranded nucleic acids or oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/532—Closed or circular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Crystallography & Structural Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP20382064 | 2020-01-31 | ||
| EP20382064.2 | 2020-01-31 | ||
| PCT/EP2021/052203 WO2021152146A1 (en) | 2020-01-31 | 2021-01-29 | Process for the production of closed linear dna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3164387A1 true CA3164387A1 (en) | 2021-08-05 |
Family
ID=69713978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3164387A Pending CA3164387A1 (en) | 2020-01-31 | 2021-01-29 | Process for the production of closed linear dna |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US12522864B2 (https=) |
| EP (1) | EP4097252A1 (https=) |
| JP (2) | JP7771066B2 (https=) |
| KR (1) | KR20220134001A (https=) |
| CN (1) | CN115023503A (https=) |
| AU (1) | AU2021212319A1 (https=) |
| CA (1) | CA3164387A1 (https=) |
| WO (1) | WO2021152146A1 (https=) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4124660A1 (en) * | 2021-07-30 | 2023-02-01 | 4basebio, S.L.U. | Linear dna with enhanced resistance against exonucleases and methods for the production thereof |
| EP4299731A1 (en) * | 2022-06-29 | 2024-01-03 | ASOCIACIÓN CENTRO DE INVESTIGACIÓN COOPERATIVA EN NANOCIENCIAS "CIC nanoGUNE" | Synthetic primase-polymerase and uses thereof |
| CN119698477A (zh) * | 2022-08-10 | 2025-03-25 | 镇江蓬勃生物科技有限公司 | 线性闭合dna的制备方法及该方法所用质粒 |
| WO2024039652A1 (en) | 2022-08-16 | 2024-02-22 | Aldevron, L.L.C. | Cell-free method of producing synthetic circular nucleic acid |
| CN116606834A (zh) * | 2023-04-10 | 2023-08-18 | 天津中合基因科技有限公司 | 多种具有切割连接活性的原端酶及其应用 |
| WO2025003977A2 (en) * | 2023-06-29 | 2025-01-02 | Seqirus Inc. | In vitro transcription method |
| EP4735631A2 (en) * | 2023-06-30 | 2026-05-06 | Novel Biotechnology USA Inc. | Cell-free replication of a nucleic acid construct |
| EP4491719A1 (en) * | 2023-07-12 | 2025-01-15 | 4basebio, S.L.U. | Methods of amplifying template nucleic acid using a thermostable tthprimpol |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4373071A (en) | 1981-04-30 | 1983-02-08 | City Of Hope Research Institute | Solid-phase synthesis of polynucleotides |
| DE19826758C1 (de) * | 1998-06-15 | 1999-10-21 | Soft Gene Gmbh | Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte |
| GB0901593D0 (en) * | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
| ES2359058B1 (es) | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
| GB201013153D0 (en) * | 2010-08-04 | 2010-09-22 | Touchlight Genetics Ltd | Primer for production of closed linear DNA |
| EP2971080B1 (en) * | 2013-03-15 | 2018-01-03 | Expedeon, S.L. | Methods for amplification and sequencing using thermostable tthprimpol |
| CN109844134B (zh) * | 2016-08-16 | 2023-05-05 | 塔驰莱特Ip有限公司 | 闭合线性dna的生产 |
| JP7026248B2 (ja) * | 2017-11-21 | 2022-02-25 | ４ベースバイオ ソシエダー リミターダ | 二本鎖ｄｎａを増幅するための方法およびキット |
| WO2019118806A1 (en) * | 2017-12-14 | 2019-06-20 | Solid Biosciences Inc. | Non-viral production and delivery of genes |
-
2021
- 2021-01-29 CN CN202180011993.0A patent/CN115023503A/zh active Pending
- 2021-01-29 EP EP21703391.9A patent/EP4097252A1/en active Pending
- 2021-01-29 CA CA3164387A patent/CA3164387A1/en active Pending
- 2021-01-29 AU AU2021212319A patent/AU2021212319A1/en active Pending
- 2021-01-29 US US17/796,596 patent/US12522864B2/en active Active
- 2021-01-29 JP JP2022545422A patent/JP7771066B2/ja active Active
- 2021-01-29 KR KR1020227030090A patent/KR20220134001A/ko active Pending
- 2021-01-29 WO PCT/EP2021/052203 patent/WO2021152146A1/en not_active Ceased
-
2025
- 2025-11-05 JP JP2025186318A patent/JP2026041724A/ja active Pending
- 2025-12-16 US US19/421,128 patent/US20260103746A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN115023503A (zh) | 2022-09-06 |
| US20260103746A1 (en) | 2026-04-16 |
| JP7771066B2 (ja) | 2025-11-17 |
| US20230075380A1 (en) | 2023-03-09 |
| JP2026041724A (ja) | 2026-03-10 |
| WO2021152146A1 (en) | 2021-08-05 |
| EP4097252A1 (en) | 2022-12-07 |
| US12522864B2 (en) | 2026-01-13 |
| KR20220134001A (ko) | 2022-10-05 |
| JP2023511991A (ja) | 2023-03-23 |
| AU2021212319A1 (en) | 2022-07-28 |
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