CA3152803A1 - Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides - Google Patents

Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides Download PDF

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Publication number
CA3152803A1
CA3152803A1 CA3152803A CA3152803A CA3152803A1 CA 3152803 A1 CA3152803 A1 CA 3152803A1 CA 3152803 A CA3152803 A CA 3152803A CA 3152803 A CA3152803 A CA 3152803A CA 3152803 A1 CA3152803 A1 CA 3152803A1
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CA
Canada
Prior art keywords
seq
polypeptide
host cell
modified host
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3152803A
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English (en)
French (fr)
Inventor
Andrew HORWITZ
Jeff Wong
Darren Platt
Jeff UBERSAX
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Demetrix Inc
Original Assignee
Demetrix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Demetrix Inc filed Critical Demetrix Inc
Publication of CA3152803A1 publication Critical patent/CA3152803A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y121/00—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21)
    • C12Y121/03—Oxidoreductases acting on X-H and Y-H to form an X-Y bond (1.21) with oxygen as acceptor (1.21.3)
    • C12Y121/03007—Tetrahydrocannabinolic acid synthase (1.21.3.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA3152803A 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides Pending CA3152803A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962902300P 2019-09-18 2019-09-18
US62/902,300 2019-09-18
PCT/US2020/051261 WO2021055597A1 (en) 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides

Publications (1)

Publication Number Publication Date
CA3152803A1 true CA3152803A1 (en) 2021-03-25

Family

ID=72744852

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3152803A Pending CA3152803A1 (en) 2019-09-18 2020-09-17 Optimized tetrahydrocannabinolic acid (thca) synthase polypeptides

Country Status (9)

Country Link
EP (1) EP4031657A1 (ja)
JP (1) JP2022548904A (ja)
CN (1) CN115038786A (ja)
AU (1) AU2020349513A1 (ja)
BR (1) BR112022004797A2 (ja)
CA (1) CA3152803A1 (ja)
IL (1) IL291328A (ja)
MX (1) MX2022003203A (ja)
WO (1) WO2021055597A1 (ja)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020176547A1 (en) 2019-02-25 2020-09-03 Ginkgo Bioworks, Inc. Biosynthesis of cannabinoids and cannabinoid precursors
WO2023168266A2 (en) * 2022-03-02 2023-09-07 Genomatica, Inc. Flavin-dependent oxidases having cannabinoid synthase activity

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60335574D1 (de) 2002-10-04 2011-02-10 Du Pont Verfahren zur biologischen herstellung von 1,3-propandiol mit hoher ausbeute
RU2545699C2 (ru) 2007-12-13 2015-04-10 ДАНИСКО ЮЭс ИНК. Композиции и способы получения изопрена
SG165884A1 (en) 2008-04-23 2010-11-29 Goodyear Tire & Rubber Isoprene synthase variants for improved microbial production of isoprene
CA2729801A1 (en) 2008-07-02 2010-01-07 Danisco Us Inc. Compositions and methods for producing isoprene free of c5 hydrocarbons under decoupling conditions and/or safe operating ranges
JP7198555B2 (ja) * 2017-04-27 2023-01-04 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア カンナビノイドおよびカンナビノイド誘導体を産生するための微生物および方法
CA3069449A1 (en) * 2017-07-12 2019-01-17 Biomedican, Inc. Production of cannabinoids in yeast
WO2019118912A1 (en) * 2017-12-14 2019-06-20 Medicinal Genomics Corporation Methods and kits for classifying cannabinoid production in cannabis plants
WO2020069214A2 (en) * 2018-09-26 2020-04-02 Demetrix, Inc. Optimized expression systems for producing cannabinoid synthase polypeptides, cannabinoids, and cannabinoid derivatives

Also Published As

Publication number Publication date
CN115038786A (zh) 2022-09-09
EP4031657A1 (en) 2022-07-27
BR112022004797A2 (pt) 2022-06-21
MX2022003203A (es) 2022-06-08
AU2020349513A1 (en) 2022-05-05
JP2022548904A (ja) 2022-11-22
IL291328A (en) 2022-05-01
WO2021055597A1 (en) 2021-03-25

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