CA3149805A1 - Improved methods for making organoid compositions - Google Patents

Improved methods for making organoid compositions Download PDF

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Publication number
CA3149805A1
CA3149805A1 CA3149805A CA3149805A CA3149805A1 CA 3149805 A1 CA3149805 A1 CA 3149805A1 CA 3149805 A CA3149805 A CA 3149805A CA 3149805 A CA3149805 A CA 3149805A CA 3149805 A1 CA3149805 A1 CA 3149805A1
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Canada
Prior art keywords
endoderm
gut
aggregates
aggregated
organoids
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Pending
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CA3149805A
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English (en)
French (fr)
Inventor
Christopher Norman MAYHEW
James Macormack Wells
Amy Lee PITSTICK
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Cincinnati Childrens Hospital Medical Center
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Cincinnati Childrens Hospital Medical Center
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Publication of CA3149805A1 publication Critical patent/CA3149805A1/en
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/237—Oncostatin M [OSM]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
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    • C12N2501/39—Steroid hormones
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases (EC 2.)
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
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  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA3149805A 2019-08-13 2020-08-11 Improved methods for making organoid compositions Pending CA3149805A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962885903P 2019-08-13 2019-08-13
US62/885,903 2019-08-13
PCT/US2020/045809 WO2021030373A1 (en) 2019-08-13 2020-08-11 Improved methods for making organoid compositions

Publications (1)

Publication Number Publication Date
CA3149805A1 true CA3149805A1 (en) 2021-02-18

Family

ID=74571192

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3149805A Pending CA3149805A1 (en) 2019-08-13 2020-08-11 Improved methods for making organoid compositions

Country Status (8)

Country Link
US (1) US20220275345A1 (zh)
EP (1) EP4013853A4 (zh)
JP (1) JP2022544175A (zh)
CN (1) CN114502721A (zh)
AU (1) AU2020329194A1 (zh)
CA (1) CA3149805A1 (zh)
IL (1) IL290544A (zh)
WO (1) WO2021030373A1 (zh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4363556A1 (en) * 2021-07-02 2024-05-08 Children's Hospital Medical Center Structurally complete organoids
CN114317415B (zh) * 2022-03-07 2022-06-03 天九再生医学(天津)科技有限公司 人多能干细胞向多谱系大肠类器官定向分化的方法
WO2024025808A1 (en) * 2022-07-29 2024-02-01 Children's Hospital Medical Center Population-scale organoid pools
WO2024063999A1 (en) * 2022-09-22 2024-03-28 Children's Hospital Medical Center Organoid compositions having immune cells
WO2024071212A1 (ja) * 2022-09-27 2024-04-04 慶應義塾 細胞外基質の非存在下でオルガノイドを培養するための薬剤

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US8859286B2 (en) * 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
US20180258400A1 (en) * 2015-09-15 2018-09-13 Agency For Science, Technology And Research (A*Star) Derivation of liver organoids from human pluripotent stem cells
CA3027317A1 (en) * 2016-06-14 2017-12-21 Cellply S.R.L. Screening kit and method
US11898166B2 (en) * 2017-09-20 2024-02-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services In vitro generation of thymic organoid from human pluripotent stem cells

Also Published As

Publication number Publication date
WO2021030373A1 (en) 2021-02-18
AU2020329194A1 (en) 2022-02-24
CN114502721A (zh) 2022-05-13
EP4013853A1 (en) 2022-06-22
EP4013853A4 (en) 2023-08-23
JP2022544175A (ja) 2022-10-17
IL290544A (en) 2022-04-01
US20220275345A1 (en) 2022-09-01

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