CA3149805A1 - Improved methods for making organoid compositions - Google Patents

Improved methods for making organoid compositions Download PDF

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Publication number
CA3149805A1
CA3149805A1 CA3149805A CA3149805A CA3149805A1 CA 3149805 A1 CA3149805 A1 CA 3149805A1 CA 3149805 A CA3149805 A CA 3149805A CA 3149805 A CA3149805 A CA 3149805A CA 3149805 A1 CA3149805 A1 CA 3149805A1
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Prior art keywords
endoderm
gut
aggregates
aggregated
organoids
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CA3149805A
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English (en)
French (fr)
Inventor
Christopher Norman MAYHEW
James Macormack Wells
Amy Lee PITSTICK
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Cincinnati Childrens Hospital Medical Center
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Cincinnati Childrens Hospital Medical Center
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Publication of CA3149805A1 publication Critical patent/CA3149805A1/en
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
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    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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    • C12M23/00—Constructional details, e.g. recesses, hinges
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA3149805A 2019-08-13 2020-08-11 Improved methods for making organoid compositions Pending CA3149805A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962885903P 2019-08-13 2019-08-13
US62/885,903 2019-08-13
PCT/US2020/045809 WO2021030373A1 (en) 2019-08-13 2020-08-11 Improved methods for making organoid compositions

Publications (1)

Publication Number Publication Date
CA3149805A1 true CA3149805A1 (en) 2021-02-18

Family

ID=74571192

Family Applications (1)

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CA3149805A Pending CA3149805A1 (en) 2019-08-13 2020-08-11 Improved methods for making organoid compositions

Country Status (8)

Country Link
US (1) US20220275345A1 (https=)
EP (1) EP4013853A4 (https=)
JP (2) JP7676361B2 (https=)
CN (1) CN114502721A (https=)
AU (1) AU2020329194A1 (https=)
CA (1) CA3149805A1 (https=)
IL (1) IL290544A (https=)
WO (1) WO2021030373A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4388083A4 (en) * 2021-08-19 2025-07-09 Childrens Hospital Med Ct VASCULARIZED ORGANOIDS
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same

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US9719068B2 (en) 2010-05-06 2017-08-01 Children's Hospital Medical Center Methods and systems for converting precursor cells into intestinal tissues through directed differentiation
SG10201801654RA (en) 2014-05-28 2018-04-27 Childrens Hospital Med Ct Methods and systems for converting precursor cells into gastric tissues through directed differentiation
EP4553082A3 (en) 2016-11-04 2025-08-20 Children's Hospital Medical Center Liver organoid compositions and methods of making and using same
JP7248586B2 (ja) 2017-04-14 2023-03-29 チルドレンズ ホスピタル メディカル センター 複数ドナー幹細胞組成物およびそれを作製する方法
EP3694603B1 (en) 2017-10-10 2026-04-08 Children's Hospital Medical Center Esophageal tissue and/or organoid compositions and methods of making same
EP3727394A4 (en) 2017-12-21 2021-09-08 Children's Hospital Medical Center DIGITALIZED HUMAN ORGANOIDS AND METHOD OF USING THEREOF
EP4678727A3 (en) 2018-07-26 2026-03-11 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CA3112026A1 (en) 2018-09-12 2020-03-19 Children's Hospital Medical Center Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
WO2023278676A1 (en) * 2021-07-02 2023-01-05 Children’S Hospital Medical Center Structurally complete organoids
CN114317415B (zh) * 2022-03-07 2022-06-03 天九再生医学(天津)科技有限公司 人多能干细胞向多谱系大肠类器官定向分化的方法
EP4562127A1 (en) * 2022-07-29 2025-06-04 Children's Hospital Medical Center Population-scale organoid pools
EP4580691A1 (en) * 2022-09-22 2025-07-09 Children's Hospital Medical Center Organoid compositions having immune cells
CN119968460A (zh) * 2022-09-27 2025-05-09 庆应义塾 用于在不存在细胞外基质的条件下培养类器官的药物
WO2024206911A2 (en) 2023-03-30 2024-10-03 Children's Hospital Medical Center Clinical-grade organoids
WO2024238977A2 (en) 2023-05-18 2024-11-21 Children's Hospital Medical Center Liver organoids with intrahepatic sympathetic nerves, and methods of use thereof
EP4731750A2 (en) 2023-06-23 2026-04-29 Children's Hospital Medical Center Methods of matrix-free suspension culture
WO2025072803A1 (en) 2023-09-29 2025-04-03 Children's Hospital Medical Center Ntrk2 signaling-mediated alveolar capillary injury and repair
WO2025212920A1 (en) 2024-04-03 2025-10-09 Children's Hospital Medical Center Multi-zonal liver organoids
WO2025217202A1 (en) 2024-04-08 2025-10-16 Children's Hospital Medical Center Bile duct organoid
WO2026015589A1 (en) * 2024-07-09 2026-01-15 Children's Hospital Medical Center Pancreatic organoids and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
WO2011119507A2 (en) * 2010-03-25 2011-09-29 International Stem Cell Corporation Method of altering the differentiative state of a cell and compositions thereof
US8859286B2 (en) * 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
EP3350313B1 (en) * 2015-09-15 2025-11-26 Agency For Science, Technology And Research (A*star) Derivation of liver organoids from human pluripotent stem cells
CA3027317A1 (en) * 2016-06-14 2017-12-21 Cellply S.R.L. Screening kit and method
US11898166B2 (en) * 2017-09-20 2024-02-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services In vitro generation of thymic organoid from human pluripotent stem cells

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12600943B2 (en) 2019-02-01 2026-04-14 The University Of Hong Kong Innervated organoid compositions and methods of making same
US12497597B2 (en) 2019-05-31 2025-12-16 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
EP4388083A4 (en) * 2021-08-19 2025-07-09 Childrens Hospital Med Ct VASCULARIZED ORGANOIDS

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Publication number Publication date
CN114502721A (zh) 2022-05-13
EP4013853A4 (en) 2023-08-23
JP7676361B2 (ja) 2025-05-14
JP2025128081A (ja) 2025-09-02
JP2022544175A (ja) 2022-10-17
WO2021030373A1 (en) 2021-02-18
US20220275345A1 (en) 2022-09-01
IL290544A (en) 2022-04-01
EP4013853A1 (en) 2022-06-22
AU2020329194A1 (en) 2022-02-24

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