CA3144955A1 - Electronic detection of a target based on enzymatic cleavage of a reporter moiety - Google Patents

Electronic detection of a target based on enzymatic cleavage of a reporter moiety Download PDF

Info

Publication number
CA3144955A1
CA3144955A1 CA3144955A CA3144955A CA3144955A1 CA 3144955 A1 CA3144955 A1 CA 3144955A1 CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A1 CA3144955 A1 CA 3144955A1
Authority
CA
Canada
Prior art keywords
biologically
channel
reporter
enzyme
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3144955A
Other languages
English (en)
French (fr)
Inventor
Kiana ARAN
Alexander Kane
Brett Goldsmith
Regis Paytavi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Paragraf USA
Original Assignee
Ammr Joint Venture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ammr Joint Venture filed Critical Ammr Joint Venture
Priority claimed from PCT/US2020/039678 external-priority patent/WO2020264204A1/en
Publication of CA3144955A1 publication Critical patent/CA3144955A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3144955A 2019-06-25 2020-06-25 Electronic detection of a target based on enzymatic cleavage of a reporter moiety Pending CA3144955A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962866312P 2019-06-25 2019-06-25
US62/866,312 2019-06-25
US201916459298A 2019-07-01 2019-07-01
US16/459,298 2019-07-01
US202063036772P 2020-06-09 2020-06-09
US63/036,772 2020-06-09
PCT/US2020/039678 WO2020264204A1 (en) 2019-06-25 2020-06-25 Electronic detection of a target based on enzymatic cleavage of a reporter moiety

Publications (1)

Publication Number Publication Date
CA3144955A1 true CA3144955A1 (en) 2020-12-30

Family

ID=79689640

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3144955A Pending CA3144955A1 (en) 2019-06-25 2020-06-25 Electronic detection of a target based on enzymatic cleavage of a reporter moiety

Country Status (4)

Country Link
EP (1) EP3990630A4 (zh)
KR (1) KR20220024917A (zh)
CN (1) CN114341339A (zh)
CA (1) CA3144955A1 (zh)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11905552B2 (en) * 2017-08-04 2024-02-20 Keck Graduate Institute Of Applied Life Sciences Immobilized RNPs for sequence-specific nucleic acid capture and digital detection

Also Published As

Publication number Publication date
CN114341339A (zh) 2022-04-12
KR20220024917A (ko) 2022-03-03
EP3990630A4 (en) 2022-08-17
EP3990630A1 (en) 2022-05-04

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