CA3144955A1 - Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur - Google Patents
Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur Download PDFInfo
- Publication number
- CA3144955A1 CA3144955A1 CA3144955A CA3144955A CA3144955A1 CA 3144955 A1 CA3144955 A1 CA 3144955A1 CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A1 CA3144955 A1 CA 3144955A1
- Authority
- CA
- Canada
- Prior art keywords
- biologically
- channel
- reporter
- enzyme
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
L'invention concerne des appareils, des systèmes et des procédés de détection de cible sur la base d'un clivage collatéral d'un rapporteur (730) par une enzyme (720). L'invention porte notamment sur un transistor à grille biologique (106) pouvant comprendre un canal (710) et un fragment rapporteur (730) immobilisé sur le canal (710). L'état de la fraction rapporteur (730) affecte des signaux de sortie provenant du transistor à grille biologique (106) lorsque des conditions d'excitation sont appliquées au transistor à grille biologique (106) et un fluide échantillon (110) est appliqué en contact avec le canal (710). Un fluide échantillon (110) peut comprendre une enzyme (720) configurée pour s'activer en réponse à un acide nucléique cible (722) pour cliver la fraction rapporteur (730). Un circuit d'excitation (1502) applique les conditions d'excitation, et un circuit de mesure (1504) mesure des signaux de sortie provenant du transistor à grille biologique (106). Un module d'analyse (116) détermine un paramètre relatif à la présence de l'acide nucléique cible (722), sur la base de la ou des mesures.
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201962866312P | 2019-06-25 | 2019-06-25 | |
US62/866,312 | 2019-06-25 | ||
US201916459298A | 2019-07-01 | 2019-07-01 | |
US16/459,298 | 2019-07-01 | ||
US202063036772P | 2020-06-09 | 2020-06-09 | |
US63/036,772 | 2020-06-09 | ||
PCT/US2020/039678 WO2020264204A1 (fr) | 2019-06-25 | 2020-06-25 | Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur |
Publications (1)
Publication Number | Publication Date |
---|---|
CA3144955A1 true CA3144955A1 (fr) | 2020-12-30 |
Family
ID=79689640
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3144955A Pending CA3144955A1 (fr) | 2019-06-25 | 2020-06-25 | Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP3990630A4 (fr) |
KR (1) | KR20220024917A (fr) |
CN (1) | CN114341339A (fr) |
CA (1) | CA3144955A1 (fr) |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11905552B2 (en) * | 2017-08-04 | 2024-02-20 | Keck Graduate Institute Of Applied Life Sciences | Immobilized RNPs for sequence-specific nucleic acid capture and digital detection |
-
2020
- 2020-06-25 EP EP20831413.8A patent/EP3990630A4/fr active Pending
- 2020-06-25 CA CA3144955A patent/CA3144955A1/fr active Pending
- 2020-06-25 CN CN202080060246.1A patent/CN114341339A/zh active Pending
- 2020-06-25 KR KR1020227002443A patent/KR20220024917A/ko active Search and Examination
Also Published As
Publication number | Publication date |
---|---|
CN114341339A (zh) | 2022-04-12 |
KR20220024917A (ko) | 2022-03-03 |
EP3990630A4 (fr) | 2022-08-17 |
EP3990630A1 (fr) | 2022-05-04 |
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