EP3990630A4 - Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur - Google Patents

Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur Download PDF

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Publication number
EP3990630A4
EP3990630A4 EP20831413.8A EP20831413A EP3990630A4 EP 3990630 A4 EP3990630 A4 EP 3990630A4 EP 20831413 A EP20831413 A EP 20831413A EP 3990630 A4 EP3990630 A4 EP 3990630A4
Authority
EP
European Patent Office
Prior art keywords
target based
enzymatic cleavage
electronic detection
reporter moiety
reporter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP20831413.8A
Other languages
German (de)
English (en)
Other versions
EP3990630A1 (fr
Inventor
Kiana ARAN
Alexander Kane
Brett Goldsmith
Regis Paytavi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Paragraf USA
Original Assignee
Ammr Joint Venture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ammr Joint Venture filed Critical Ammr Joint Venture
Priority claimed from PCT/US2020/039678 external-priority patent/WO2020264204A1/fr
Publication of EP3990630A1 publication Critical patent/EP3990630A1/fr
Publication of EP3990630A4 publication Critical patent/EP3990630A4/fr
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP20831413.8A 2019-06-25 2020-06-25 Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur Pending EP3990630A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201962866312P 2019-06-25 2019-06-25
US201916459298A 2019-07-01 2019-07-01
US202063036772P 2020-06-09 2020-06-09
PCT/US2020/039678 WO2020264204A1 (fr) 2019-06-25 2020-06-25 Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur

Publications (2)

Publication Number Publication Date
EP3990630A1 EP3990630A1 (fr) 2022-05-04
EP3990630A4 true EP3990630A4 (fr) 2022-08-17

Family

ID=79689640

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20831413.8A Pending EP3990630A4 (fr) 2019-06-25 2020-06-25 Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur

Country Status (4)

Country Link
EP (1) EP3990630A4 (fr)
KR (1) KR20220024917A (fr)
CN (1) CN114341339A (fr)
CA (1) CA3144955A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190112643A1 (en) * 2017-08-04 2019-04-18 Keck Graduate Institute Immobilized RNPs for sequence-specific nucleic acid capture and digital detection

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010033087A1 (fr) * 2008-09-19 2010-03-25 Nanyang Technological University Dispositif électronique comportant un canal, des électrodes et un semi-conducteur formés sur des substrats collés respectifs
US11215580B2 (en) * 2014-04-28 2022-01-04 Cardea Bio, Inc. System and method for DNA sequencing and blood chemistry analysis
WO2017201081A1 (fr) * 2016-05-16 2017-11-23 Agilome, Inc. Dispositifs à fet au graphène, systèmes et leurs méthodes d'utilisation pour le séquençage d'acides nucléiques
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190112643A1 (en) * 2017-08-04 2019-04-18 Keck Graduate Institute Immobilized RNPs for sequence-specific nucleic acid capture and digital detection

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HAJIAN REZA ET AL: "Detection of unamplified target genes via CRISPR-Cas9 immobilized on a graphene field-effect transistor", NATURE BIOMEDICAL ENGINEERING, NATURE PUBLISHING GROUP UK, LONDON, vol. 3, no. 6, 25 March 2019 (2019-03-25), pages 427 - 437, XP036799471, DOI: 10.1038/S41551-019-0371-X *
LI YI ET AL: "CRISPR/Cas Systems towards Next-Generation Biosensing", TRENDS IN BIOTECHNOLOGY, vol. 37, no. 7, 1 July 2019 (2019-07-01), pages 730 - 743, XP085710951, ISSN: 0167-7799, DOI: 10.1016/J.TIBTECH.2018.12.005 *
See also references of WO2020264204A1 *
SHI-YUAN LI ET AL: "CRISPR-Cas12a-assisted nucleic acid detection", CELL DISCOVERY, vol. 4, no. 1, 24 April 2018 (2018-04-24), XP055538799, DOI: 10.1038/s41421-018-0028-z *

Also Published As

Publication number Publication date
EP3990630A1 (fr) 2022-05-04
CA3144955A1 (fr) 2020-12-30
CN114341339A (zh) 2022-04-12
KR20220024917A (ko) 2022-03-03

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RIC1 Information provided on ipc code assigned before grant

Ipc: C12Q 1/6825 20180101ALI20220712BHEP

Ipc: C12Q 1/6811 20180101ALI20220712BHEP

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Ipc: C12N 15/10 20060101ALI20220712BHEP

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DAV Request for validation of the european patent (deleted)
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RIN1 Information on inventor provided before grant (corrected)

Inventor name: PEYTAVI, REGIS

Inventor name: GOLDSMITH, BRETT

Inventor name: KANE, ALEXANDER

Inventor name: ARAN, KIANA

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CARDEA BIO, INC.