CA3142188A1 - Hyperspectral quantitative imaging cytometry system - Google Patents

Hyperspectral quantitative imaging cytometry system Download PDF

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Publication number
CA3142188A1
CA3142188A1 CA3142188A CA3142188A CA3142188A1 CA 3142188 A1 CA3142188 A1 CA 3142188A1 CA 3142188 A CA3142188 A CA 3142188A CA 3142188 A CA3142188 A CA 3142188A CA 3142188 A1 CA3142188 A1 CA 3142188A1
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CA
Canada
Prior art keywords
sample
radiation
wavelength
hyperspectral
coded
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3142188A
Other languages
English (en)
French (fr)
Inventor
Jose Mario Tenera Morgado
Alvaro Rodriguez De La Gala
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytognos SL
Original Assignee
Cytognos SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cytognos SL filed Critical Cytognos SL
Publication of CA3142188A1 publication Critical patent/CA3142188A1/en
Pending legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J3/26—Generating the spectrum; Monochromators using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/30—Measuring the intensity of spectral lines directly on the spectrum itself
    • G01J3/32—Investigating bands of a spectrum in sequence by a single detector
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/02—Details
    • G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
    • G01J2003/102—Plural sources
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1226—Interference filters
    • G01J2003/123—Indexed discrete filters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1226—Interference filters
    • G01J2003/1234—Continuously variable IF [CVIF]; Wedge type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/12—Generating the spectrum; Monochromators
    • G01J2003/1226—Interference filters
    • G01J2003/1243—Pivoting IF or other position variation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/2823—Imaging spectrometer
    • G01J2003/2826—Multispectral imaging, e.g. filter imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/144—Imaging characterised by its optical setup

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Signal Processing (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
CA3142188A 2019-05-31 2020-05-29 Hyperspectral quantitative imaging cytometry system Pending CA3142188A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19382444 2019-05-31
EP19382444.8 2019-05-31
PCT/EP2020/064984 WO2020239981A1 (en) 2019-05-31 2020-05-29 Hyperspectral quantitative imaging cytometry system

Publications (1)

Publication Number Publication Date
CA3142188A1 true CA3142188A1 (en) 2020-12-03

Family

ID=66912761

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3142188A Pending CA3142188A1 (en) 2019-05-31 2020-05-29 Hyperspectral quantitative imaging cytometry system

Country Status (7)

Country Link
US (1) US12078585B2 (https=)
EP (1) EP3977091B1 (https=)
JP (1) JP2022535798A (https=)
CN (2) CN120084706A (https=)
AU (1) AU2020281945B2 (https=)
CA (1) CA3142188A1 (https=)
WO (1) WO2020239981A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12493965B2 (en) * 2020-12-11 2025-12-09 Enumera Molecular, Inc. Methods and systems for image processing
US20230221178A1 (en) * 2022-01-11 2023-07-13 Modulight Corporation Apparatus and a method for fluorescence imaging
CN116309497B (zh) * 2023-03-26 2023-10-03 湖南医药学院 基于图像识别的癌细胞计数及预后预测的辅助分析方法

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3819187B2 (ja) * 1999-09-28 2006-09-06 富士写真フイルム株式会社 マルチバンド画像の分光反射率のスペクトル推定方法およびスペクトル推定システム
WO2002068932A2 (en) 2001-02-23 2002-09-06 Genicon Sciences Corporation Methods for providing extended dynamic range in analyte assays
WO2004111930A2 (en) 2003-05-30 2004-12-23 Rabinovich Andrew M Color unmixing and region of interest detection in tissue samples
US7554659B2 (en) * 2004-06-30 2009-06-30 Chemimage Corporation Hyperspectral visible absorption imaging of molecular probes and dyes in biomaterials
EP2024893B1 (en) 2006-05-12 2015-03-04 Janssen Diagnostics, LLC A laser illumination system in fluorescent microscopy
JP2012508366A (ja) 2008-11-04 2012-04-05 ウィリアム・マーシュ・ライス・ユニバーシティ 画像マッピング分光計
JP5570798B2 (ja) * 2009-12-15 2014-08-13 オリンパス株式会社 光走査型内視鏡装置
US10180398B2 (en) 2012-02-29 2019-01-15 National Technology & Engineering Solutions Of Sandia, Llc Trajectory-based triggering system for hyperspectral imaging flow cytometer
US9797836B1 (en) * 2012-02-29 2017-10-24 National Technology & Engineering Solutions Of Sandia, Llc Hyperspectral imaging flow cytometer
US20150297142A1 (en) * 2012-07-30 2015-10-22 Koninklijke Philips N.V. Device and method for extracting physiological information
US9494567B2 (en) * 2012-12-31 2016-11-15 Omni Medsci, Inc. Near-infrared lasers for non-invasive monitoring of glucose, ketones, HBA1C, and other blood constituents
US9733124B2 (en) * 2013-04-18 2017-08-15 BMG LABTECH, GmbH Microplate reader with linear variable filter
CN104516085B (zh) * 2013-09-30 2017-04-26 香港纺织及成衣研发中心有限公司 一种用于多光谱成像的快速调焦方法及装置
GB201415238D0 (en) * 2014-08-28 2014-10-15 Renishaw Plc Spectroscopy apparatus
US10120180B2 (en) * 2014-09-28 2018-11-06 Massachusetts Institute Of Technology Methods and apparatus for stretched light field microscope
ES2569550A1 (es) 2014-10-10 2016-05-11 Consejo Superior De Investigaciones Científicas (Csic) Espectrofotómetro
JP5901814B1 (ja) * 2015-03-25 2016-04-13 日本分光株式会社 顕微分光装置
JP2017064405A (ja) 2015-09-29 2017-04-06 住友電気工業株式会社 光学測定装置及び光学測定方法
US10018560B2 (en) 2016-02-02 2018-07-10 Kla-Tencor Corporation System and method for hyperspectral imaging metrology
EP3940436B1 (en) 2016-02-15 2025-12-17 Leica Instruments (Singapore) Pte. Ltd. Illumination filter system and observation system for a multispectral fluorescence microscope, multispectral fluorescence microscope, and microscopying method
US10502621B2 (en) * 2016-12-27 2019-12-10 Urugus S.A. Dynamic hyper-spectral imaging of objects in apparent motion
EP3555580B1 (en) * 2016-12-27 2023-12-06 Urugus S.A. Hyper-spectral imaging when observed object is still
WO2020161826A1 (ja) * 2019-02-06 2020-08-13 オリンパス株式会社 撮像装置

Also Published As

Publication number Publication date
WO2020239981A1 (en) 2020-12-03
AU2020281945A1 (en) 2021-12-16
US12078585B2 (en) 2024-09-03
CN120084706A (zh) 2025-06-03
CN113906284A (zh) 2022-01-07
US20220228968A1 (en) 2022-07-21
AU2020281945B2 (en) 2025-06-05
JP2022535798A (ja) 2022-08-10
EP3977091A1 (en) 2022-04-06
EP3977091B1 (en) 2025-12-31

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