CA3142179A1 - Systems and methods for fabricating a microchannel vascular network device and seeding a microchannel - Google Patents

Systems and methods for fabricating a microchannel vascular network device and seeding a microchannel

Info

Publication number
CA3142179A1
CA3142179A1 CA3142179A CA3142179A CA3142179A1 CA 3142179 A1 CA3142179 A1 CA 3142179A1 CA 3142179 A CA3142179 A CA 3142179A CA 3142179 A CA3142179 A CA 3142179A CA 3142179 A1 CA3142179 A1 CA 3142179A1
Authority
CA
Canada
Prior art keywords
microchannel
vascular network
network device
channel
gelma
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3142179A
Other languages
English (en)
French (fr)
Inventor
Joseph P. Vacanti
Batzaya Byambaa
Carly COMER
Matthew James Hancock
Tyler LIEBERTHAL
Tatevik SAHAKYANTS
Andrew Paul SPANN
Craig Neville
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
3D Biolabs LLC
Original Assignee
3D Biolabs LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3D Biolabs LLC filed Critical 3D Biolabs LLC
Publication of CA3142179A1 publication Critical patent/CA3142179A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
    • B29C64/30—Auxiliary operations or equipment
    • B29C64/386—Data acquisition or data processing for additive manufacturing
    • B29C64/393—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B33—ADDITIVE MANUFACTURING TECHNOLOGY
    • B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
    • B33Y50/00—Data acquisition or data processing for additive manufacturing
    • B33Y50/02—Data acquisition or data processing for additive manufacturing for controlling or regulating additive manufacturing processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2529/00—Culture process characterised by the use of electromagnetic stimulation
    • C12N2529/10—Stimulation by light
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Materials Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Manufacturing & Machinery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Molecular Biology (AREA)
  • Optics & Photonics (AREA)
  • Mechanical Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Prostheses (AREA)
  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
CA3142179A 2019-06-18 2020-06-18 Systems and methods for fabricating a microchannel vascular network device and seeding a microchannel Pending CA3142179A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962863165P 2019-06-18 2019-06-18
US201962863169P 2019-06-18 2019-06-18
US62/863,165 2019-06-18
US62/863,169 2019-06-18
PCT/US2020/038560 WO2020257539A1 (en) 2019-06-18 2020-06-18 Systems and methods for fabricating a microchannel vascular network device and seeding a microchannel

Publications (1)

Publication Number Publication Date
CA3142179A1 true CA3142179A1 (en) 2020-12-24

Family

ID=71575801

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3142179A Pending CA3142179A1 (en) 2019-06-18 2020-06-18 Systems and methods for fabricating a microchannel vascular network device and seeding a microchannel

Country Status (10)

Country Link
US (2) US12351827B2 (https=)
EP (1) EP3987003A1 (https=)
JP (1) JP2022536892A (https=)
KR (1) KR20220024635A (https=)
CN (1) CN114222819B (https=)
AU (1) AU2020294779B2 (https=)
BR (1) BR112021025507A2 (https=)
CA (1) CA3142179A1 (https=)
SG (1) SG11202113099QA (https=)
WO (1) WO2020257539A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119072541A (zh) 2022-04-22 2024-12-03 3D生物实验室公司 用于组织装置的液密箱以及具有所述组织装置的系统
WO2024073071A1 (en) * 2022-09-29 2024-04-04 The University Of Akron Chamber assembly for ex-vivo electroretinogram with filleted perfusion inlet and outlet
WO2026050767A2 (en) 2024-08-30 2026-03-05 3D Biolabs, Llc Systems and methods for fabricating hydrogels with intrinsic vascular channels for liver tissue engineering

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6176874B1 (en) 1993-10-18 2001-01-23 Masschusetts Institute Of Technology Vascularized tissue regeneration matrices formed by solid free form fabrication techniques
US7759113B2 (en) * 1999-04-30 2010-07-20 The General Hospital Corporation Fabrication of tissue lamina using microfabricated two-dimensional molds
US6772026B2 (en) * 2000-04-05 2004-08-03 Therics, Inc. System and method for rapidly customizing design, manufacture and/or selection of biomedical devices
ES2859575T3 (es) * 2007-04-12 2021-10-04 Massachusetts Gen Hospital Red vascular biomimética
WO2009042671A1 (en) * 2007-09-24 2009-04-02 The Board Of Trustees Of The University Of Illinois Three-dimensional microfabricated bioreactors with embedded capillary network
ES2672201T3 (es) 2008-07-16 2018-06-13 Children's Medical Center Corporation Dispositivo de imitación de órganos con microcanales y métodos de uso
CA2966623C (en) 2014-11-03 2024-02-20 The General Hospital Corporation Concentrating particles in a microfluidic device
KR101709312B1 (ko) 2015-03-11 2017-02-23 서강대학교산학협력단 하이드로젤 기반의 세포 공동-배양용 미세유체칩
WO2016154070A1 (en) 2015-03-20 2016-09-29 William Marsh Rice University Hypothermic 3d bioprinting of living tissues supported by perfusable vasculature
KR102530274B1 (ko) * 2015-07-06 2023-05-08 어드밴스드 솔루션즈 라이프 사이언스, 엘엘씨 혈관화된 시험관 관류 장치, 제조 방법 및 그 응용 방법
EP3359642A4 (en) 2015-10-06 2019-05-08 University of Pittsburgh- Of the Commonwealth System of Higher Education MICROFLUIDIC TISSUE DEVELOPMENT SYSTEMS
US11326138B2 (en) 2017-05-01 2022-05-10 University Of Kentucky Research Foundation Cell culture device and methods of use thereof

Also Published As

Publication number Publication date
KR20220024635A (ko) 2022-03-03
SG11202113099QA (en) 2021-12-30
AU2020294779B2 (en) 2026-02-19
JP2022536892A (ja) 2022-08-22
EP3987003A1 (en) 2022-04-27
US12351827B2 (en) 2025-07-08
BR112021025507A2 (pt) 2022-04-26
WO2020257539A1 (en) 2020-12-24
CN114222819B (zh) 2025-02-25
AU2020294779A1 (en) 2021-12-23
US20260009004A1 (en) 2026-01-08
US20210071145A1 (en) 2021-03-11
CN114222819A (zh) 2022-03-22

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