CA3107560A1 - Esterases and uses thereof - Google Patents

Esterases and uses thereof Download PDF

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Publication number
CA3107560A1
CA3107560A1 CA3107560A CA3107560A CA3107560A1 CA 3107560 A1 CA3107560 A1 CA 3107560A1 CA 3107560 A CA3107560 A CA 3107560A CA 3107560 A CA3107560 A CA 3107560A CA 3107560 A1 CA3107560 A1 CA 3107560A1
Authority
CA
Canada
Prior art keywords
esterase
seq
substitutions
polyester
amino acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3107560A
Other languages
English (en)
French (fr)
Inventor
Sophie Duquesne
Vincent TOURNIER
Alain Marty
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Carbios SA
Original Assignee
Carbios SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbios SA filed Critical Carbios SA
Publication of CA3107560A1 publication Critical patent/CA3107560A1/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J11/00—Recovery or working-up of waste materials
    • C08J11/04—Recovery or working-up of waste materials of polymers
    • C08J11/10—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation
    • C08J11/105—Recovery or working-up of waste materials of polymers by chemically breaking down the molecular chains of polymers or breaking of crosslinks, e.g. devulcanisation by treatment with enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074—Cutinase (3.1.1.74)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2367/00—Characterised by the use of polyesters obtained by reactions forming a carboxylic ester link in the main chain; Derivatives of such polymers
    • C08J2367/02—Polyesters derived from dicarboxylic acids and dihydroxy compounds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/50—Reuse, recycling or recovery technologies
    • Y02W30/62—Plastics recycling; Rubber recycling

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CA3107560A 2018-07-27 2019-07-26 Esterases and uses thereof Pending CA3107560A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP18306020 2018-07-27
EP18306020.1 2018-07-27
EP18306447.6 2018-11-05
EP18306447 2018-11-05
PCT/EP2019/070289 WO2020021118A1 (en) 2018-07-27 2019-07-26 Novel esterases and uses thereof

Publications (1)

Publication Number Publication Date
CA3107560A1 true CA3107560A1 (en) 2020-01-30

Family

ID=67396962

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3107560A Pending CA3107560A1 (en) 2018-07-27 2019-07-26 Esterases and uses thereof

Country Status (8)

Country Link
US (1) US11851689B2 (cg-RX-API-DMAC7.html)
EP (1) EP3830271A1 (cg-RX-API-DMAC7.html)
JP (1) JP7601756B2 (cg-RX-API-DMAC7.html)
KR (1) KR102876883B1 (cg-RX-API-DMAC7.html)
CN (2) CN112639101B (cg-RX-API-DMAC7.html)
CA (1) CA3107560A1 (cg-RX-API-DMAC7.html)
MX (1) MX2021001045A (cg-RX-API-DMAC7.html)
WO (1) WO2020021118A1 (cg-RX-API-DMAC7.html)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN116286728B (zh) 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
US11692181B2 (en) 2018-07-27 2023-07-04 Carbios Esterases and uses thereof
EP4424823B1 (en) 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof
FR3088070B1 (fr) * 2018-11-06 2021-11-26 Carbios Procede de degradation enzymatique de polyethylene terephtalate
US12492308B2 (en) 2019-03-28 2025-12-09 Carbios Multicomponent thermoplastic product
EP3997220B1 (en) 2019-07-11 2025-09-10 Carbios Esterases and uses thereof
CN114096664B (zh) 2019-07-11 2025-03-07 卡比奥斯公司 新型酯酶及其用途
EP3838976B1 (en) 2019-12-19 2025-02-12 Carbios Process for degrading plastic products
GB202005073D0 (en) 2020-04-06 2020-05-20 Mellizyme Biotechnology Ltd Enzymatic degradation of plastics
JP2023546501A (ja) 2020-10-27 2023-11-02 キャルビオス 新規エステラーゼ及びその使用
CN118994721A (zh) 2021-05-20 2024-11-22 卡比奥斯公司 降解包含至少一种聚酯的塑料产品的方法
US20240228731A1 (en) 2021-05-20 2024-07-11 Carbios Process for degrading a plastic product comprising at least one polyester
CN117730090A (zh) 2021-07-13 2024-03-19 诺维信公司 重组角质酶表达
MX2024001814A (es) * 2021-08-11 2024-03-01 Biometis Tech Inc Degradacion enzimatica de polietilentereftalato.
EP4433582A2 (en) * 2021-11-16 2024-09-25 Carbios Esterases and uses thereof
WO2023088910A1 (en) * 2021-11-16 2023-05-25 Carbios Use of esterases
CN118355115A (zh) * 2021-11-16 2024-07-16 卡比奥斯公司 酯酶及其用途
CN114891767B (zh) * 2022-05-19 2023-04-28 华南理工大学 角质酶-酯酶融合蛋白及其应用
TW202411323A (zh) 2022-06-13 2024-03-16 法商卡爾畢歐斯公司 純化單乙二醇之方法
EP4321616A1 (en) 2022-08-09 2024-02-14 Covestro Deutschland AG Thermally stable carboxylic ester hydrolases in layered double hydroxides for intrinsic recyclable polymers
KR20260011167A (ko) 2023-05-15 2026-01-22 까르비오 신규 에스테라제 및 이의 용도
KR20260011740A (ko) 2023-05-15 2026-01-23 까르비오 신규 에스테라제 및 이의 용도
IT202300014223A1 (it) * 2023-07-07 2025-01-07 Milano Politecnico Enzima ingegnerizzato termostabile
WO2025128934A1 (en) * 2023-12-15 2025-06-19 Flagship Pioneering Innovations Vii, Llc Esterases and related methods

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5850342B2 (ja) * 2011-01-19 2016-02-03 天野エンザイム株式会社 枝葉コンポスト由来新規エステラーゼ
FR2984354A1 (fr) 2011-12-20 2013-06-21 Centre Nat Rech Scient Procede de preparation d'alliage polymere/enzymes
TR201900782T4 (tr) 2012-11-20 2019-02-21 Carbios Plastik ürünlerin geri dönüştürülmesine yönelik yöntem.
US20160280881A1 (en) 2013-11-05 2016-09-29 Carbios A method for degrading a plastic
CN122012456A (zh) 2013-12-11 2026-05-12 诺维信公司 角质酶变体以及对其进行编码的多核苷酸
ES2924896T3 (es) 2014-05-16 2022-10-11 Carbios Procedimiento para reciclar artículos de plástico mixto con PET
EP3778883A1 (en) 2014-10-21 2021-02-17 Carbios Polypeptide having a polyester degrading activity and uses thereof
US10626242B2 (en) 2014-12-19 2020-04-21 Carbios Plastic compound and preparation process
CN107532153A (zh) 2015-03-13 2018-01-02 卡比奥斯公司 具有聚酯降解活性的新多肽及其用途
CA2987842C (en) 2015-06-12 2024-01-02 Carbios Biodegradable polyester composition and uses thereof
EP3394264A1 (en) 2015-12-21 2018-10-31 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
PL3458508T3 (pl) 2016-05-19 2026-02-23 Carbios Sposób degradowania produktów z tworzyw sztucznych
CN116286728B (zh) * 2016-07-12 2026-04-24 卡比奥斯公司 新酯酶及其用途
CN114891768B (zh) * 2016-07-12 2024-09-24 卡比奥斯公司 新酯酶及其用途
CN115927269A (zh) 2016-12-16 2023-04-07 卡比奥斯公司 增加塑料降解的蛋白酶
KR20230131950A (ko) 2017-08-31 2023-09-14 카르비오리스 효소를 포함하는 생분해성 폴리에스테르 물품
MX2020002290A (es) 2017-08-31 2020-07-13 Carbios Composicion liquida que comprende entidades biologicas y usos de la misma.
CA3084107A1 (en) 2017-12-21 2019-06-27 Carbios Novel proteases and uses thereof
EP4424823B1 (en) 2018-07-27 2025-12-03 Carbios Novel esterases and uses thereof
US11692181B2 (en) 2018-07-27 2023-07-04 Carbios Esterases and uses thereof

Also Published As

Publication number Publication date
US20210180037A1 (en) 2021-06-17
WO2020021118A1 (en) 2020-01-30
US11851689B2 (en) 2023-12-26
KR102876883B1 (ko) 2025-10-27
MX2021001045A (es) 2021-04-12
JP7601756B2 (ja) 2024-12-17
CN112639101B (zh) 2025-03-18
KR20210043599A (ko) 2021-04-21
EP3830271A1 (en) 2021-06-09
JP2021531031A (ja) 2021-11-18
CN120098957A (zh) 2025-06-06
CN112639101A (zh) 2021-04-09

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