CA3103739A1 - Polymerases, compositions, and methods of use - Google Patents
Polymerases, compositions, and methods of use Download PDFInfo
- Publication number
- CA3103739A1 CA3103739A1 CA3103739A CA3103739A CA3103739A1 CA 3103739 A1 CA3103739 A1 CA 3103739A1 CA 3103739 A CA3103739 A CA 3103739A CA 3103739 A CA3103739 A CA 3103739A CA 3103739 A1 CA3103739 A1 CA 3103739A1
- Authority
- CA
- Canada
- Prior art keywords
- polymerase
- amino acid
- mutation
- functionally equivalent
- position functionally
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862775662P | 2018-12-05 | 2018-12-05 | |
| US62/775,662 | 2018-12-05 | ||
| PCT/US2019/064524 WO2020117968A2 (en) | 2018-12-05 | 2019-12-04 | Polymerases, compositions, and methods of use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3103739A1 true CA3103739A1 (en) | 2020-06-11 |
Family
ID=69137980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3103739A Pending CA3103739A1 (en) | 2018-12-05 | 2019-12-04 | Polymerases, compositions, and methods of use |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US11001816B2 (enExample) |
| EP (1) | EP3891275A2 (enExample) |
| JP (1) | JP7527208B2 (enExample) |
| KR (1) | KR20210098844A (enExample) |
| CN (1) | CN112639090A (enExample) |
| AU (1) | AU2019391093B2 (enExample) |
| BR (1) | BR112020026655A2 (enExample) |
| CA (1) | CA3103739A1 (enExample) |
| IL (2) | IL279423B (enExample) |
| MX (1) | MX2020013357A (enExample) |
| MY (1) | MY204767A (enExample) |
| SG (1) | SG11202012559SA (enExample) |
| WO (1) | WO2020117968A2 (enExample) |
| ZA (1) | ZA202007839B (enExample) |
Families Citing this family (52)
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| EP3891275A2 (en) | 2018-12-05 | 2021-10-13 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
| MY208607A (en) | 2019-11-27 | 2025-05-20 | Illumina Cambridge Ltd | Cyclooctatetraene containing dyes and compositions |
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| CN115667507A (zh) | 2019-12-31 | 2023-01-31 | 奇异基因组学系统公司 | 用于长读出测序的多核苷酸条形码 |
| EP4117682B1 (en) | 2020-04-24 | 2025-06-04 | Singular Genomics Systems, Inc. | Modified nucleotides and uses thereof |
| IL297992B2 (en) | 2020-06-22 | 2025-03-01 | Illumina Cambridge Ltd | Nucleosides and nucleotides with 3' acetal blocking group |
| US20220195516A1 (en) | 2020-12-17 | 2022-06-23 | Illumina Cambridge Limited | Methods, systems and compositions for nucleic acid sequencing |
| US20220195518A1 (en) | 2020-12-22 | 2022-06-23 | Illumina Cambridge Limited | Methods and compositions for nucleic acid sequencing |
| US12054506B2 (en) | 2021-03-19 | 2024-08-06 | Singular Genomics Systems, Inc. | Reducing agents and uses thereof |
| KR102884006B1 (ko) | 2021-05-05 | 2025-11-10 | 일루미나 케임브리지 리미티드 | 비스-붕소 융합 헤테로사이클을 함유하는 형광 염료 및 서열분석에서의 용도 |
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| US11242512B2 (en) | 2018-09-17 | 2022-02-08 | Omniome, Inc. | Engineered polymerases for improved sequencing |
| JP7515413B2 (ja) | 2018-10-31 | 2024-07-12 | イラミーナ インコーポレーテッド | ポリメラーゼ、組成物、および使用方法 |
| EP3891275A2 (en) | 2018-12-05 | 2021-10-13 | Illumina, Inc. | Polymerases, compositions, and methods of use |
| US11293061B2 (en) | 2018-12-26 | 2022-04-05 | Illumina Cambridge Limited | Sequencing methods using nucleotides with 3′ AOM blocking group |
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2019
- 2019-12-04 EP EP19832480.8A patent/EP3891275A2/en active Pending
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- 2019-12-04 WO PCT/US2019/064524 patent/WO2020117968A2/en not_active Ceased
- 2019-12-04 CA CA3103739A patent/CA3103739A1/en active Pending
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| US20200181587A1 (en) | 2020-06-11 |
| ZA202007839B (en) | 2022-04-28 |
| JP7527208B2 (ja) | 2024-08-02 |
| KR20210098844A (ko) | 2021-08-11 |
| AU2019391093A1 (en) | 2021-01-07 |
| BR112020026655A2 (pt) | 2021-04-06 |
| SG11202012559SA (en) | 2021-01-28 |
| US11001816B2 (en) | 2021-05-11 |
| WO2020117968A3 (en) | 2020-08-13 |
| IL279423A (en) | 2021-01-31 |
| US20210348141A1 (en) | 2021-11-11 |
| EP3891275A2 (en) | 2021-10-13 |
| MX2020013357A (es) | 2021-04-28 |
| US11634697B2 (en) | 2023-04-25 |
| US20250059521A1 (en) | 2025-02-20 |
| US20230357731A1 (en) | 2023-11-09 |
| IL279423B (en) | 2022-08-01 |
| AU2019391093B2 (en) | 2025-11-27 |
| JP2022511206A (ja) | 2022-01-31 |
| US12104182B2 (en) | 2024-10-01 |
| WO2020117968A2 (en) | 2020-06-11 |
| CN112639090A (zh) | 2021-04-09 |
| MY204767A (en) | 2024-09-12 |
| IL297994A (en) | 2025-01-01 |
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