CA3103736A1 - Methods and compositions for cluster generation by bridge amplification - Google Patents
Methods and compositions for cluster generation by bridge amplification Download PDFInfo
- Publication number
- CA3103736A1 CA3103736A1 CA3103736A CA3103736A CA3103736A1 CA 3103736 A1 CA3103736 A1 CA 3103736A1 CA 3103736 A CA3103736 A CA 3103736A CA 3103736 A CA3103736 A CA 3103736A CA 3103736 A1 CA3103736 A1 CA 3103736A1
- Authority
- CA
- Canada
- Prior art keywords
- stranded
- amplification
- nucleic acids
- nucleic acid
- site
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2497—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing N- glycosyl compounds (3.2.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/301—Endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
- C12Q2521/319—Exonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/543—Detection characterised by immobilisation to a surface characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862775679P | 2018-12-05 | 2018-12-05 | |
| US62/775,679 | 2018-12-05 | ||
| PCT/EP2019/083173 WO2020114918A1 (en) | 2018-12-05 | 2019-11-29 | Methods and compositions for cluster generation by bridge amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3103736A1 true CA3103736A1 (en) | 2020-06-11 |
Family
ID=68771646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3103736A Pending CA3103736A1 (en) | 2018-12-05 | 2019-11-29 | Methods and compositions for cluster generation by bridge amplification |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11667953B2 (enExample) |
| EP (1) | EP3891305B1 (enExample) |
| JP (1) | JP7569690B2 (enExample) |
| KR (1) | KR102872251B1 (enExample) |
| CN (1) | CN112654718B (enExample) |
| AU (1) | AU2019391274B2 (enExample) |
| CA (1) | CA3103736A1 (enExample) |
| ES (1) | ES3074791T3 (enExample) |
| SG (1) | SG11202012762WA (enExample) |
| WO (1) | WO2020114918A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2019391274B2 (en) | 2018-12-05 | 2025-10-30 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
| CN112739830A (zh) | 2018-12-18 | 2021-04-30 | 伊卢米纳剑桥有限公司 | 使用单一表面引物进行配对末端测序的方法和组合物 |
| BR112022019541A2 (pt) | 2020-03-30 | 2022-11-16 | Illumina Inc | Métodos e composições para preparação de bibliotecas de ácido nucleico |
| CN114457146B (zh) * | 2022-01-28 | 2024-11-19 | 赛纳生物科技(北京)有限公司 | 一种固相介质表面双端扩增测序的方法 |
| US20240401129A1 (en) * | 2023-05-31 | 2024-12-05 | Illumina, Inc. | Methods for double-stranded sequencing by synthesis |
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| WO2018197950A1 (en) | 2017-04-23 | 2018-11-01 | Illumina Cambridge Limited | Compositions and methods for improving sample identification in indexed nucleic acid libraries |
| AU2019391274B2 (en) | 2018-12-05 | 2025-10-30 | Illumina Cambridge Limited | Methods and compositions for cluster generation by bridge amplification |
| CN112739830A (zh) | 2018-12-18 | 2021-04-30 | 伊卢米纳剑桥有限公司 | 使用单一表面引物进行配对末端测序的方法和组合物 |
-
2019
- 2019-11-29 AU AU2019391274A patent/AU2019391274B2/en active Active
- 2019-11-29 CA CA3103736A patent/CA3103736A1/en active Pending
- 2019-11-29 ES ES19813785T patent/ES3074791T3/es active Active
- 2019-11-29 SG SG11202012762WA patent/SG11202012762WA/en unknown
- 2019-11-29 CN CN201980042543.0A patent/CN112654718B/zh active Active
- 2019-11-29 WO PCT/EP2019/083173 patent/WO2020114918A1/en not_active Ceased
- 2019-11-29 EP EP19813785.3A patent/EP3891305B1/en active Active
- 2019-11-29 KR KR1020207037049A patent/KR102872251B1/ko active Active
- 2019-11-29 JP JP2020572458A patent/JP7569690B2/ja active Active
- 2019-12-03 US US16/702,169 patent/US11667953B2/en active Active
-
2023
- 2023-04-26 US US18/139,689 patent/US20230295687A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3891305B1 (en) | 2026-06-03 |
| KR102872251B1 (ko) | 2025-10-16 |
| US11667953B2 (en) | 2023-06-06 |
| WO2020114918A1 (en) | 2020-06-11 |
| US20200181686A1 (en) | 2020-06-11 |
| AU2019391274A1 (en) | 2021-01-07 |
| KR20210098842A (ko) | 2021-08-11 |
| JP2022511207A (ja) | 2022-01-31 |
| EP3891305A1 (en) | 2021-10-13 |
| AU2019391274B2 (en) | 2025-10-30 |
| ES3074791T3 (es) | 2026-07-23 |
| CN112654718A (zh) | 2021-04-13 |
| CN112654718B (zh) | 2025-07-22 |
| JP7569690B2 (ja) | 2024-10-18 |
| EP3891305C0 (en) | 2026-06-03 |
| SG11202012762WA (en) | 2021-01-28 |
| US20230295687A1 (en) | 2023-09-21 |
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