CA3065384A1 - Creation et utilisation d'acides nucleiques guides - Google Patents
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- CA3065384A1 CA3065384A1 CA3065384A CA3065384A CA3065384A1 CA 3065384 A1 CA3065384 A1 CA 3065384A1 CA 3065384 A CA3065384 A CA 3065384A CA 3065384 A CA3065384 A CA 3065384A CA 3065384 A1 CA3065384 A1 CA 3065384A1
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- C12N15/09—Recombinant DNA-technology
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- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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Abstract
L'invention concerne des procédés et des compositions pour fabriquer des acides nucléiques guides (ANg), des acides nucléiques codant pour des ANg, des collections d'ANg et des acides nucléiques codant pour une collection d'ANg à partir de n'importe quel acide nucléique source. L'invention concerne également des procédés et des compositions pour utiliser lesdits ANg, lesdits acides nucléiques codant pour des ANg, lesdites collections d'ANg et lesdits acides nucléiques codant pour une collection d'ANg dans diverses applications.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762516619P | 2017-06-07 | 2017-06-07 | |
| US62/516,619 | 2017-06-07 | ||
| US201762548036P | 2017-08-21 | 2017-08-21 | |
| US62/548,036 | 2017-08-21 | ||
| PCT/US2018/036563 WO2018227025A1 (fr) | 2017-06-07 | 2018-06-07 | Création et utilisation d'acides nucléiques guides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3065384A1 true CA3065384A1 (fr) | 2018-12-13 |
Family
ID=64566018
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3065384A Pending CA3065384A1 (fr) | 2017-06-07 | 2018-06-07 | Creation et utilisation d'acides nucleiques guides |
Country Status (7)
| Country | Link |
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| US (1) | US20200190508A1 (fr) |
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| CN109310784B (zh) | 2015-12-07 | 2022-08-19 | 阿克生物公司 | 用于制备和使用指导核酸的方法和组合物 |
| EP4296373A3 (fr) | 2018-10-04 | 2024-01-24 | Arc Bio, LLC | Commandes de normalisation pour gérer de faibles entrées d'échantillon dans le séquençage de nouvelle génération |
| EP3953471A4 (fr) * | 2019-04-09 | 2023-02-01 | Arc Bio, LLC | Compositions et méthodes de déplétion basée sur une modification nucléotidique |
| WO2021142197A1 (fr) * | 2020-01-09 | 2021-07-15 | Duke University | Compositions et méthodes de synthèse d'adn activé par crispr |
| AU2021246531A1 (en) * | 2020-04-02 | 2022-11-24 | Altius Institute For Biomedical Sciences | Methods, compositions, and kits for identifying regions of genomic DNA bound to a protein |
| WO2021257453A2 (fr) * | 2020-06-15 | 2021-12-23 | The Broad Institute, Inc. | Séquençage de réseau d'amplicons chimériques |
| WO2022148450A1 (fr) * | 2021-01-08 | 2022-07-14 | Wuhan University | Compositions et procédés de détection d'acide nucléique instantanée |
| CN113462755B (zh) * | 2021-05-06 | 2023-03-21 | 中国人民解放军陆军军医大学第一附属医院 | 一种用于短链非编码rna检测的模块化酶电路检测系统 |
| CN114293264A (zh) * | 2021-12-21 | 2022-04-08 | 翌圣生物科技(上海)股份有限公司 | 酶法靶序列随机sgRNA文库的制备方法 |
| WO2023158739A2 (fr) * | 2022-02-17 | 2023-08-24 | Claret Bioscience, Llc | Procédés et compositions d'analyse d'acide nucléique |
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| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| US8183359B2 (en) * | 2007-03-01 | 2012-05-22 | Gen-Probe Incorporated | Kits for amplifying DNA |
| US8323930B2 (en) * | 2007-07-28 | 2012-12-04 | Dna Twopointo, Inc. | Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase |
| US8029993B2 (en) * | 2008-04-30 | 2011-10-04 | Population Genetics Technologies Ltd. | Asymmetric adapter library construction |
| CA2879018A1 (fr) * | 2012-07-13 | 2014-01-16 | X-Chem, Inc. | Banques encodees par l'adn possedant des liaisons oligonucleotidiques codantes ne pouvant etre lues par les polymerases |
| SG10201912327SA (en) * | 2012-12-12 | 2020-02-27 | Broad Inst Inc | Engineering and Optimization of Improved Systems, Methods and Enzyme Compositions for Sequence Manipulation |
| WO2014165549A1 (fr) * | 2013-04-01 | 2014-10-09 | University Of Florida Research Foundation, Incorporated | Détermination de l'état de méthylation et de la structure chromatinienne de loci génétiques cibles |
| CA2913236A1 (fr) * | 2013-06-07 | 2014-12-11 | Keygene N.V. | Methode de sequencage cible |
| CN105637097A (zh) * | 2013-08-05 | 2016-06-01 | 特韦斯特生物科学公司 | 从头合成的基因文库 |
| WO2015050919A1 (fr) * | 2013-10-01 | 2015-04-09 | Life Technologies Corporation | Systèmes et procédés permettant de détecter des variants structuraux |
| WO2016054032A1 (fr) * | 2014-09-29 | 2016-04-07 | The Jackson Laboratory | Génération extrêmement efficace et à rendement élevé de mammifères génétiquement modifiés par électroporation |
| AU2015346514B2 (en) * | 2014-11-11 | 2021-04-08 | Illumina, Inc. | Polynucleotide amplification using CRISPR-Cas systems |
| JP6947638B2 (ja) * | 2014-12-20 | 2021-10-13 | アーク バイオ， エルエルシー | Ｃｒｉｓｐｒ／ｃａｓ系タンパク質を使用する核酸の標的化枯渇、富化および分割のための組成物および方法 |
| ES2955957T3 (es) * | 2015-01-28 | 2023-12-11 | Caribou Biosciences Inc | Polinucleótidos de ADN/ARN híbridos CRISPR y procedimientos de uso |
| WO2016130697A1 (fr) * | 2015-02-11 | 2016-08-18 | Memorial Sloan Kettering Cancer Center | Méthodes et kits pour générer des vecteurs qui coexpriment de multiples molécules cibles |
| CN107250383B (zh) * | 2015-02-17 | 2022-09-06 | 深圳华大智造科技股份有限公司 | 使用受控的链置换的dna序列测定 |
| US20160362680A1 (en) * | 2015-05-15 | 2016-12-15 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US11279926B2 (en) * | 2015-06-05 | 2022-03-22 | The Regents Of The University Of California | Methods and compositions for generating CRISPR/Cas guide RNAs |
| CA2995983A1 (fr) * | 2015-08-19 | 2017-02-23 | Arc Bio, Llc | Capture d'acides nucleiques a l'aide d'un systeme utilisant une nuclease guidee par des acides nucleiques |
| CA3001314A1 (fr) * | 2015-10-08 | 2017-04-13 | President And Fellows Of Harvard College | Edition genomique multiplexee d'elements retroviraux |
| JP2018532419A (ja) | 2015-11-09 | 2018-11-08 | イフォム・フォンダツィオーネ・イスティトゥート・フィルチ・ディ・オンコロジア・モレコラーレ | ＣＲＩＳＰＲ−Ｃａｓ ｓｇＲＮＡライブラリー |
| CN109310784B (zh) * | 2015-12-07 | 2022-08-19 | 阿克生物公司 | 用于制备和使用指导核酸的方法和组合物 |
| US20200255823A1 (en) * | 2016-10-06 | 2020-08-13 | Pioneer Biolabs, Llc | Guide strand library construction and methods of use thereof |
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| AU2018279112A1 (en) | 2019-12-19 |
| JP7282692B2 (ja) | 2023-05-29 |
| EP3635114A4 (fr) | 2021-03-17 |
| WO2018227025A1 (fr) | 2018-12-13 |
| CN111094565B (zh) | 2024-02-06 |
| CN111094565A (zh) | 2020-05-01 |
| US20200190508A1 (en) | 2020-06-18 |
| JP2020524491A (ja) | 2020-08-20 |
| EP3635114A1 (fr) | 2020-04-15 |
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