CA3054730A1 - Differentiation and use of human microglia-like cells from pluripotent stem cells and hematopoietic progenitors - Google Patents

Differentiation and use of human microglia-like cells from pluripotent stem cells and hematopoietic progenitors Download PDF

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Publication number
CA3054730A1
CA3054730A1 CA3054730A CA3054730A CA3054730A1 CA 3054730 A1 CA3054730 A1 CA 3054730A1 CA 3054730 A CA3054730 A CA 3054730A CA 3054730 A CA3054730 A CA 3054730A CA 3054730 A1 CA3054730 A1 CA 3054730A1
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imgls
media
cells
human
microglia
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English (en)
French (fr)
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Mathew BLURTON-JONES
Edsel ABUD
Wayne POON
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University of California San Diego UCSD
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0622—Glial cells, e.g. astrocytes, oligodendrocytes; Schwann cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0306—Animal model for genetic diseases
    • A01K2267/0312—Animal model for Alzheimer's disease
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0306—Animal model for genetic diseases
    • A01K2267/0318—Animal model for neurodegenerative disease, e.g. non- Alzheimer's
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • C—CHEMISTRY; METALLURGY
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/12—Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Veterinary Medicine (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA3054730A 2017-02-28 2018-02-26 Differentiation and use of human microglia-like cells from pluripotent stem cells and hematopoietic progenitors Pending CA3054730A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762464925P 2017-02-28 2017-02-28
US62/464,925 2017-02-28
PCT/US2018/019763 WO2018160496A1 (en) 2017-02-28 2018-02-26 Differentiation and use of human microglia-like cells from pluripotent stem cells and hematopoietic progenitors

Publications (1)

Publication Number Publication Date
CA3054730A1 true CA3054730A1 (en) 2018-09-07

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CA3054730A Pending CA3054730A1 (en) 2017-02-28 2018-02-26 Differentiation and use of human microglia-like cells from pluripotent stem cells and hematopoietic progenitors

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US (2) US20200239844A1 (https=)
EP (2) EP4272551A3 (https=)
JP (3) JP2020513794A (https=)
CA (1) CA3054730A1 (https=)
WO (1) WO2018160496A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12421517B2 (en) 2021-04-01 2025-09-23 The Regents Of The University Of California Genetic modification of mammalian cells to confer resistance to CSF1R antagonists
US12612599B2 (en) 2019-09-10 2026-04-28 Savanna Biotherapeutics, Inc. Compositions and methods for microglia replacement therapy

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GB201619876D0 (en) 2016-11-24 2017-01-11 Cambridge Entpr Ltd Controllable transcription
JOP20190248A1 (ar) 2017-04-21 2019-10-20 Amgen Inc بروتينات ربط مولد ضد trem2 واستخداماته
CA3114651A1 (en) * 2018-09-28 2020-04-02 Memorial Sloan-Kettering Cancer Center Stem cell-derived human microglial cells, methods of making and methods of use
US12577531B2 (en) 2019-03-29 2026-03-17 Agency For Science, Technology And Research Microglia-sufficient brain organoids
WO2020243657A1 (en) * 2019-05-30 2020-12-03 President And Fellows Of Harvard College Reproducible brain organoids and methods of making
GB2584664B (en) * 2019-06-10 2023-05-24 Newcells Biotech Ltd Improved retinal organoids and methods of making the same
CN110205295B (zh) * 2019-06-25 2021-10-29 中国科学院动物研究所 一种多能干细胞诱导产生小胶质细胞的方法及试剂盒
KR20210077634A (ko) * 2019-12-17 2021-06-25 코아스템(주) 인간 만능 줄기세포로부터 3d 오가노이드를 이용하여 미세교세포를 다량 확보하는 미세교세포의 분화방법
CN112011509A (zh) * 2020-09-25 2020-12-01 南京中医药大学 脊髓损伤大鼠原代小胶质细胞分离方法及应用
CN114426951B (zh) * 2020-10-29 2024-05-14 北京赛尔湃腾科技咨询合伙企业(有限合伙) 利用多能干细胞制备小胶质细胞样细胞的方法
US20260108560A1 (en) * 2022-07-13 2026-04-23 The Regents Of The University Of California Transplanting stem cell-derived microglia to treat leukodystrophies
US20240269189A1 (en) 2022-12-19 2024-08-15 FUJIFILM Holdings America Corporation Extracellular vesicle-enriched secretome composition derived from induced pluripotent stem cell derived-microglia and methods of use thereof

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CA2759978C (en) * 2009-04-28 2020-06-02 Life & Brain Gmbh Method for obtaining human microglial precursor cells from pluripotent stem cells
CA2972598C (en) * 2014-12-31 2024-03-12 Wisconsin Alumni Research Foundation Human pluripotent stem cell-based models for predictive developmental neural toxicity
US10081792B2 (en) * 2014-12-31 2018-09-25 Wisconsin Alumni Research Foundation Derivation of human microglia from pluripotent stem cells
AU2016211671B2 (en) * 2015-01-26 2022-05-26 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
US11136548B2 (en) * 2015-06-24 2021-10-05 Whitehead Institute For Biomedical Research Culture medium for generating microglia from pluripotent stem cells and related methods
EP3423564A4 (en) * 2016-03-03 2019-08-21 New York Stem Cell Foundation, Inc. MICROGLIA DERIVED FROM PLURIPOTENTIAL STEM CELLS AND METHOD FOR THE PRODUCTION AND USE THEREOF

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12612599B2 (en) 2019-09-10 2026-04-28 Savanna Biotherapeutics, Inc. Compositions and methods for microglia replacement therapy
US12421517B2 (en) 2021-04-01 2025-09-23 The Regents Of The University Of California Genetic modification of mammalian cells to confer resistance to CSF1R antagonists

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JP2023182587A (ja) 2023-12-26
US20200239844A1 (en) 2020-07-30
JP2023071728A (ja) 2023-05-23
EP3589293A1 (en) 2020-01-08
JP7668551B2 (ja) 2025-04-25
EP4272551A2 (en) 2023-11-08
JP2020513794A (ja) 2020-05-21
EP4272551A3 (en) 2024-03-06
US20240417684A1 (en) 2024-12-19
EP3589293A4 (en) 2020-12-16
WO2018160496A1 (en) 2018-09-07

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