CA3047330A1 - Modified crispr rna and uses thereof - Google Patents
Modified crispr rna and uses thereof Download PDFInfo
- Publication number
- CA3047330A1 CA3047330A1 CA3047330A CA3047330A CA3047330A1 CA 3047330 A1 CA3047330 A1 CA 3047330A1 CA 3047330 A CA3047330 A CA 3047330A CA 3047330 A CA3047330 A CA 3047330A CA 3047330 A1 CA3047330 A1 CA 3047330A1
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- CA
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- Prior art keywords
- compound
- modified
- crrna
- recognition portion
- sugar moiety
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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Classifications
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/315—Phosphorothioates
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
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- C12N2310/32—Chemical structure of the sugar
- C12N2310/322—2'-R Modification
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/32—Chemical structure of the sugar
- C12N2310/323—Chemical structure of the sugar modified ring structure
- C12N2310/3231—Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/34—Spatial arrangement of the modifications
- C12N2310/346—Spatial arrangement of the modifications having a combination of backbone and sugar modifications
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- C—CHEMISTRY; METALLURGY
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/352—Nature of the modification linked to the nucleic acid via a carbon atom
- C12N2310/3521—Methyl
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/353—Nature of the modification linked to the nucleic acid via an atom other than carbon
- C12N2310/3533—Halogen
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2320/00—Applications; Uses
- C12N2320/50—Methods for regulating/modulating their activity
- C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662439828P | 2016-12-28 | 2016-12-28 | |
| US62/439,828 | 2016-12-28 | ||
| US201762491818P | 2017-04-28 | 2017-04-28 | |
| US62/491,818 | 2017-04-28 | ||
| US201762572361P | 2017-10-13 | 2017-10-13 | |
| US62/572,361 | 2017-10-13 | ||
| PCT/US2017/068642 WO2018125964A1 (en) | 2016-12-28 | 2017-12-28 | Modified crispr rna and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3047330A1 true CA3047330A1 (en) | 2018-07-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3047330A Abandoned CA3047330A1 (en) | 2016-12-28 | 2017-12-28 | Modified crispr rna and uses thereof |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20190382751A1 (OSRAM) |
| EP (1) | EP3562942A4 (OSRAM) |
| JP (1) | JP2020503017A (OSRAM) |
| AU (1) | AU2017388379A1 (OSRAM) |
| CA (1) | CA3047330A1 (OSRAM) |
| WO (1) | WO2018125964A1 (OSRAM) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11866726B2 (en) | 2017-07-14 | 2024-01-09 | Editas Medicine, Inc. | Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites |
| CA3104856A1 (en) | 2018-06-29 | 2020-01-02 | Editas Medicine, Inc. | Synthetic guide molecules, compositions and methods relating thereto |
| CN114269912A (zh) * | 2019-06-14 | 2022-04-01 | 阿伯生物技术公司 | 新型crispr dna靶向酶及系统 |
| KR20230090337A (ko) * | 2020-10-19 | 2023-06-21 | 카리부 바이오사이언시스 인코포레이티드 | Crispr 유형 v 시스템을 위한 dna-함유 폴리뉴클레오티드 및 가이드, 및 그의 제조 및 사용 방법 |
| AU2023254100A1 (en) * | 2022-04-13 | 2024-09-19 | Caribou Biosciences, Inc. | Therapeutic applications of crispr type v systems |
| AU2024270764A1 (en) | 2023-05-15 | 2025-12-04 | Nchroma Bio, Inc. | Compositions and methods for epigenetic regulation of hbv gene expression |
| CN116732075B (zh) * | 2023-06-09 | 2024-03-08 | 江南大学 | 一种生产2′-岩藻糖基乳糖的多层动态调控系统及其应用 |
| WO2025212120A1 (en) * | 2024-04-05 | 2025-10-09 | Arbor Biotechnologies, Inc. | Chemical modifications of guide rnas for crispr nucleases |
| WO2025231432A1 (en) * | 2024-05-03 | 2025-11-06 | Caribou Biosciences, Inc. | In vivo gene editing with crispr systems |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR102210319B1 (ko) * | 2013-03-15 | 2021-02-01 | 더 제너럴 하스피탈 코포레이션 | 특정 게놈 좌위에 대한 유전적 및 후성적 조절 단백질의 rna-안내 표적화 |
| US20150376587A1 (en) * | 2014-06-25 | 2015-12-31 | Caribou Biosciences, Inc. | RNA Modification to Engineer Cas9 Activity |
| EP4400584A3 (en) * | 2014-12-03 | 2024-10-16 | Agilent Technologies, Inc. | Guide rna with chemical modifications |
| KR102319192B1 (ko) * | 2015-01-28 | 2021-10-28 | 카리부 바이오사이언시스 인코포레이티드 | Crispr 하이브리드 dna/rna 폴리뉴클레오티드 및 사용 방법 |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| EP4545544A3 (en) * | 2015-06-29 | 2025-10-08 | Ionis Pharmaceuticals, Inc. | Modified crispr rna and modified single crispr rna and uses thereof |
| US12065667B2 (en) * | 2016-04-16 | 2024-08-20 | Ohio State Innovation Foundation | Modified Cpf1 MRNA, modified guide RNA, and uses thereof |
| CN106244591A (zh) * | 2016-08-23 | 2016-12-21 | 苏州吉玛基因股份有限公司 | 修饰crRNA在CRISPR/Cpf1基因编辑系统中的应用 |
| US11136567B2 (en) * | 2016-11-22 | 2021-10-05 | Integrated Dna Technologies, Inc. | CRISPR/CPF1 systems and methods |
-
2017
- 2017-12-28 JP JP2019533110A patent/JP2020503017A/ja active Pending
- 2017-12-28 AU AU2017388379A patent/AU2017388379A1/en not_active Abandoned
- 2017-12-28 US US16/470,464 patent/US20190382751A1/en not_active Abandoned
- 2017-12-28 WO PCT/US2017/068642 patent/WO2018125964A1/en not_active Ceased
- 2017-12-28 CA CA3047330A patent/CA3047330A1/en not_active Abandoned
- 2017-12-28 EP EP17886910.3A patent/EP3562942A4/en not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP3562942A1 (en) | 2019-11-06 |
| WO2018125964A1 (en) | 2018-07-05 |
| JP2020503017A (ja) | 2020-01-30 |
| EP3562942A4 (en) | 2020-12-09 |
| US20190382751A1 (en) | 2019-12-19 |
| AU2017388379A1 (en) | 2019-06-06 |
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Effective date: 20230629 |