CA3037190A1 - Methods of nucleic acid sample preparation for analysis of cell-free dna - Google Patents

Methods of nucleic acid sample preparation for analysis of cell-free dna Download PDF

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Publication number
CA3037190A1
CA3037190A1 CA3037190A CA3037190A CA3037190A1 CA 3037190 A1 CA3037190 A1 CA 3037190A1 CA 3037190 A CA3037190 A CA 3037190A CA 3037190 A CA3037190 A CA 3037190A CA 3037190 A1 CA3037190 A1 CA 3037190A1
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CA
Canada
Prior art keywords
nucleic acid
target
adapter
double
primer
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Pending
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CA3037190A
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English (en)
French (fr)
Inventor
Joshua STAHL
Jason Myers
Brady CULVER
Brian KUDLOW
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ArcherDx LLC
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ArcherDx LLC
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Publication of CA3037190A1 publication Critical patent/CA3037190A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3037190A 2016-09-15 2017-09-15 Methods of nucleic acid sample preparation for analysis of cell-free dna Pending CA3037190A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662395347P 2016-09-15 2016-09-15
US62/395,347 2016-09-15
PCT/US2017/051927 WO2018053365A1 (en) 2016-09-15 2017-09-15 Methods of nucleic acid sample preparation for analysis of cell-free dna

Publications (1)

Publication Number Publication Date
CA3037190A1 true CA3037190A1 (en) 2018-03-22

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA3037190A Pending CA3037190A1 (en) 2016-09-15 2017-09-15 Methods of nucleic acid sample preparation for analysis of cell-free dna

Country Status (7)

Country Link
US (2) US10683531B2 (enExample)
EP (2) EP4353830A3 (enExample)
JP (1) JP6997773B2 (enExample)
CN (1) CN110023504B (enExample)
AU (1) AU2017328953B2 (enExample)
CA (1) CA3037190A1 (enExample)
WO (1) WO2018053365A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4026912A1 (en) 2012-05-10 2022-07-13 The General Hospital Corporation Methods for determining a nucleotide sequence
CN105190656B (zh) 2013-01-17 2018-01-16 佩索纳里斯公司 用于遗传分析的方法和系统
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
US10125399B2 (en) 2014-10-30 2018-11-13 Personalis, Inc. Methods for using mosaicism in nucleic acids sampled distal to their origin
US11299783B2 (en) 2016-05-27 2022-04-12 Personalis, Inc. Methods and systems for genetic analysis
US12630864B2 (en) * 2016-09-15 2026-05-19 Laboratory Corporation Of America Holdings Methods of nucleic acid sample preparation
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
CN109937254B (zh) 2016-09-15 2023-05-30 阿谢尔德克斯有限责任公司 核酸样品制备方法
CA3042391A1 (en) * 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing
US11814750B2 (en) 2018-05-31 2023-11-14 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
US10801064B2 (en) 2018-05-31 2020-10-13 Personalis, Inc. Compositions, methods and systems for processing or analyzing multi-species nucleic acid samples
CN114026249B (zh) * 2019-06-20 2024-01-23 深圳华大生命科学研究院 基于rna样本构建文库的方法及应用
JP7470787B2 (ja) 2019-11-05 2024-04-18 パーソナリス，インコーポレイティド 単一試料からの腫瘍純度の推定
CN114686453A (zh) * 2020-12-28 2022-07-01 广东菲鹏生物有限公司 一种构建转录组测序文库的方法及试剂盒
WO2022204321A1 (en) 2021-03-24 2022-09-29 Ambry Genetics Corporation Conservative concurrent evaluation of dna modifications
WO2023059654A1 (en) 2021-10-05 2023-04-13 Personalis, Inc. Customized assays for personalized cancer monitoring
USD1140078S1 (en) 2022-09-02 2026-08-04 Becton, Dickinson And Company Test device
USD1069156S1 (en) 2023-04-10 2025-04-01 Becton, Dickinson And Company Dispensing device
WO2026006629A1 (en) 2024-06-27 2026-01-02 Personalis, Inc. System and method for management of compressed sequencing files

Family Cites Families (122)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5157032A (en) 1985-01-18 1992-10-20 The Trustees Of Columbia University In The City Of New York Mixed ligand complexes and uses thereof as binding agents and probes to DNA
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US6027913A (en) 1988-01-28 2000-02-22 Sommer; Steven S. Nucleic acid amplification with direct sequencing
US5374524A (en) 1988-05-10 1994-12-20 E. I. Du Pont De Nemours And Company Solution sandwich hybridization, capture and detection of amplified nucleic acids
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US6087101A (en) 1990-05-18 2000-07-11 Gruelich; Karl Otto Optical characterization of nucleic acids and oligonucleotides
PT100379B (pt) 1991-04-10 1999-01-29 Scripps Research Inst Bibliotecas de receptores heterodimericos usando fagomideos
US5604098A (en) 1993-03-24 1997-02-18 Molecular Biology Resources, Inc. Methods and materials for restriction endonuclease applications
US6063566A (en) 1994-05-13 2000-05-16 The Scripps Research Institute Catalytic RNA molecules
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5556771A (en) 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
WO1997023646A1 (en) 1995-12-22 1997-07-03 Behringwerke Aktiengesellschaft Detection of differences in nucleic acids
CA2239683A1 (en) 1995-12-22 1997-07-03 Dade Behring Marburg Gmbh Homogeneous amplification and detection of nucleic acids
US5827658A (en) 1996-08-09 1998-10-27 The United States Of America As Reprsented By The Department Of Health And Human Services Isolation of amplified genes via cDNA subtractive hybridization
US5861251A (en) 1996-10-15 1999-01-19 Bioneer Corporation Lyophilized reagent for polymerase chain reaction
AU4938497A (en) 1996-11-12 1998-06-03 Visible Genetics Inc. Method and kit for direct isothermal sequencing of nucleic acids
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US20020061532A1 (en) 1997-02-14 2002-05-23 Mosaic Technologies, Inc. Method and apparatus for performing amplification of nucleic acids on supports
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
US6197554B1 (en) 1998-11-20 2001-03-06 Shi-Lung Lin Method for generating full-length cDNA library from single cells
US6200757B1 (en) 1999-01-19 2001-03-13 Dade Behring Inc. Method for controlling the extension of an oligonucleotide
US7074556B2 (en) 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
WO2000070095A2 (en) 1999-05-17 2000-11-23 Dade Behring Inc. Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
ATE262595T1 (de) 1999-09-13 2004-04-15 Nugen Technologies Inc Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6309836B1 (en) 1999-10-05 2001-10-30 Marek Kwiatkowski Compounds for protecting hydroxyls and methods for their use
US20030022318A1 (en) 2000-01-25 2003-01-30 Epiclone, Inc. Method for thermocycling amplification of nucleic acid sequences and the generation of related peptides thereof
AU5741101A (en) * 2000-04-28 2001-11-12 Digital Gene Technologies, Inc. Methods for rapid isolation and sequence determination of gene-specific sequences
JP2001333800A (ja) * 2000-05-30 2001-12-04 Unitech Kk Ｒｎａ量およびｄｎａ量の比較検出方法
EP1356094B1 (en) 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2002213096B2 (en) 2000-10-06 2007-03-29 Nugen Technologies, Inc. Methods and probes for detection and/or quantification of nucleic acid sequences
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
EP1427847B1 (en) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US20040058373A1 (en) 2001-01-31 2004-03-25 Winkler Matthew M. Competitive amplification of fractionated targets from multiple nucleic acid samples
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
US6632611B2 (en) 2001-07-20 2003-10-14 Affymetrix, Inc. Method of target enrichment and amplification
US20030104432A1 (en) 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
WO2003078645A2 (en) 2002-03-11 2003-09-25 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3’ single stranded portion and uses of these complexes for recombination
EP1490514A4 (en) 2002-03-29 2008-12-17 Nugen Technologies Inc THROUGH ISOTHERME SINGLE PRIMER NUCLEIC ACID AMPLIFICATION IMPROVED DETECTION AND QUANTIFICATION OF ANALYTES
WO2004011665A2 (en) 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
US7273730B2 (en) 2002-05-24 2007-09-25 Invitrogen Corporation Nested PCR employing degradable primers
US6852494B2 (en) 2003-01-10 2005-02-08 Linden Technologies, Inc. Nucleic acid amplification
EP1454992B1 (en) 2003-03-07 2006-05-31 Istituto Nazionale Per Lo Studio E La Cura Dei Tumori Anaplastic lymphoma kinase assay, reagents and compositions thereof
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
EP1668158B1 (en) 2003-08-11 2010-10-20 Georgia State University Research Foundation, Inc. Rna detection and quantitation
US7226720B2 (en) 2003-09-08 2007-06-05 General Electric Company Limited play data storage media and method for limiting access to data thereon
US7824856B2 (en) 2003-09-10 2010-11-02 Althea Technologies, Inc. Expression profiling using microarrays
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20080021205A1 (en) 2003-12-11 2008-01-24 Helen Blau Methods and Compositions for Use in Preparing Hairpin Rnas
JP5138938B2 (ja) 2003-12-19 2013-02-06 プレキシコン インコーポレーテッド Ｒｅｔ調節剤の開発のための化合物および方法
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
GB0421525D0 (en) 2004-09-28 2004-10-27 Novartis Ag Inhibitors of protein kineses
US20070070349A1 (en) 2005-09-23 2007-03-29 Helicos Biosciences Corporation Optical train and method for TIRF single molecule detection and analysis
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
WO2006119280A2 (en) 2005-05-03 2006-11-09 Handylab, Inc. Lyophilized pellets
EP1929046B1 (en) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
EP1957667A1 (en) 2005-11-15 2008-08-20 Solexa Ltd. Method of target enrichment
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20090275486A1 (en) 2006-05-16 2009-11-05 Nurith Kurn Nucleic acid separation and purification method based on reversible charge interactions
WO2008005459A2 (en) 2006-06-30 2008-01-10 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
EP2121983A2 (en) 2007-02-02 2009-11-25 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8685899B2 (en) 2007-02-14 2014-04-01 Genisphere Inc. Methods, reagents and kits for detection of nucleic acid molecules
US20090093378A1 (en) * 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
US20100248310A1 (en) 2007-10-22 2010-09-30 Monoquant Pty Ltd. Method of dna amplification
JP5685085B2 (ja) 2008-01-14 2015-03-18 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー リボ核酸を検出するための組成物、方法およびキット
EP2247727A4 (en) 2008-02-12 2011-08-03 Nugen Technologies Inc PROCESS FOR ARCHIVING AND CLONING EXPANSION
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
WO2009133466A2 (en) 2008-04-30 2009-11-05 Population Genetics Technologies Ltd. Asymmetric adapter library construction
CN101270395A (zh) 2008-05-08 2008-09-24 重庆大学 柑桔衰退病毒一步法实时荧光反转录聚合酶链式反应固相化试剂盒及其检测方法
US20110172105A1 (en) 2008-06-04 2011-07-14 Salk Institute For Biological Studies GREPSEQ: An Almost Inexhaustible, Cost-Effective, High-Throughput Protocol for the Generation of Selector Sequences
EP2313091A4 (en) 2008-07-14 2012-04-04 Univ Kingston PHARMACEUTICAL COMPOSITIONS WITH RET-HEMMERN AND METHOD FOR THE TREATMENT OF CANCER
WO2010077288A2 (en) 2008-12-09 2010-07-08 The Salk Institute For Biological Studies Methods for identifying differences in alternative splicing between two rna samples
JP2010143860A (ja) 2008-12-19 2010-07-01 Chisso Corp タンパク質の安定化剤
WO2010083046A2 (en) 2009-01-15 2010-07-22 The Salk Institute For Biological Studies Methods for using next generation sequencing to identify 5-methyl cytosines in the genome
US20100286143A1 (en) 2009-04-24 2010-11-11 Dora Dias-Santagata Methods and materials for genetic analysis of tumors
US9416409B2 (en) 2009-07-31 2016-08-16 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
EP2464738A4 (en) 2009-08-12 2013-05-01 Nugen Technologies Inc METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID
WO2011032053A1 (en) 2009-09-11 2011-03-17 Nugen Technologies, Inc. Compositions and methods for whole transcriptome analysis
GB2487341A (en) 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
US20120021460A1 (en) 2010-01-08 2012-01-26 Qiagen Gaithersburg Inc. Materials and methods for isothermal nucleic acid amplification
ES2704701T3 (es) * 2010-01-19 2019-03-19 Verinata Health Inc Nuevo protocolo de preparación de bibliotecas de secuenciación
GB201008125D0 (en) 2010-05-14 2010-06-30 Biofortuna Ltd Tissue typing assays and kits
WO2011156529A2 (en) 2010-06-08 2011-12-15 Nugen Technologies, Inc. Methods and composition for multiplex sequencing
WO2012003374A2 (en) 2010-07-02 2012-01-05 The Board Of Trustees Of The Leland Stanford Junior University Targeted sequencing library preparation by genomic dna circularization
WO2012024658A2 (en) * 2010-08-20 2012-02-23 IntegenX, Inc. Integrated analysis system
AU2011305445B2 (en) 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US20120100549A1 (en) 2010-10-01 2012-04-26 Ibis Biosciences, Inc. Targeted genome amplification methods
AU2011348100B2 (en) 2010-12-22 2016-08-25 Natera, Inc. Methods for non-invasive prenatal paternity testing
WO2012103154A1 (en) 2011-01-24 2012-08-02 Nugen Technologies, Inc. Stem-loop composite rna-dna adaptor-primers: compositions and methods for library generation, amplification and other downstream manipulations
US8722585B2 (en) 2011-05-08 2014-05-13 Yan Wang Methods of making di-tagged DNA libraries from DNA or RNA using double-tagged oligonucleotides
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013074833A1 (en) 2011-11-16 2013-05-23 The Board Of Trustees Of The Leland Stanford Junior University Capture probe and assay for analysis of fragmented nucleic acids
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
EP4026912A1 (en) 2012-05-10 2022-07-13 The General Hospital Corporation Methods for determining a nucleotide sequence
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
JP6433893B2 (ja) * 2012-07-03 2018-12-05 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｔｍ増強ブロッキングオリゴヌクレオチド、ならびに標的濃縮の改善およびオフターゲット選択の低減のためのベイト
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014047678A1 (en) * 2012-09-25 2014-04-03 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
US20150291953A1 (en) 2012-11-02 2015-10-15 Enzymatics Inc. Methods and kits for nucleic acid sample preparation for sequencing
EP2964789A4 (en) 2013-03-06 2016-11-02 Ohio State Innovation Foundation ISOTHERMIC NUCLEIC ACID AMPLIFICATION AND LIBRARY GENERATION AND CLONING GENERATION IN SEQUENCING
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
WO2014144495A1 (en) 2013-03-15 2014-09-18 Abvitro, Inc. Single cell bar-coding for antibody discovery
US20140274729A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Methods, compositions and kits for generation of stranded rna or dna libraries
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
US9689027B2 (en) 2013-11-15 2017-06-27 The Board Of Trustees Of The Leland Stanford Junior University High efficiency multiplexed nucleic acid capture in a structured microenvironment
IL274464B2 (en) * 2013-12-11 2023-08-01 Accuragen Holdings Ltd Preparations and methods for the detection of rare sequence variants
US10407720B2 (en) * 2013-12-15 2019-09-10 Academia Sinica Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
AU2015209079B2 (en) 2014-01-27 2021-07-15 Archerdx, Llc Methods of preparing nucleic acids for sequencing
CN113388664A (zh) 2014-01-27 2021-09-14 阿谢尔德克斯有限公司 用于制备核酸的等温方法及相关组合物
US9738897B2 (en) 2014-06-23 2017-08-22 Regeneron Pharmaceuticals, Inc. Nuclease-mediated DNA assembly
US10590483B2 (en) 2014-09-15 2020-03-17 Abvitro Llc High-throughput nucleotide library sequencing
EP3262189B1 (en) 2015-02-27 2021-12-08 Becton, Dickinson and Company Methods for barcoding nucleic acids for sequencing
CN107922970B (zh) 2015-08-06 2021-09-28 豪夫迈·罗氏有限公司 通过单探针引物延伸的靶标富集
WO2017177308A1 (en) 2016-04-15 2017-10-19 University Health Network (Uhn) Hybrid-capture sequencing for determining immune cell clonality
CN109937254B (zh) 2016-09-15 2023-05-30 阿谢尔德克斯有限责任公司 核酸样品制备方法
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
CA3042391A1 (en) 2016-11-02 2018-05-11 ArcherDX, Inc. Methods of nucleic acid sample preparation for immune repertoire sequencing

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