CA2984701C - Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays - Google Patents
Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays Download PDFInfo
- Publication number
- CA2984701C CA2984701C CA2984701A CA2984701A CA2984701C CA 2984701 C CA2984701 C CA 2984701C CA 2984701 A CA2984701 A CA 2984701A CA 2984701 A CA2984701 A CA 2984701A CA 2984701 C CA2984701 C CA 2984701C
- Authority
- CA
- Canada
- Prior art keywords
- composition
- enzyme inactivation
- enzyme
- detection
- biological sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B15/00—Peroxides; Peroxyhydrates; Peroxyacids or salts thereof; Superoxides; Ozonides
- C01B15/01—Hydrogen peroxide
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B21/00—Nitrogen; Compounds thereof
- C01B21/08—Hydrazoic acid; Azides; Halogen azides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C55/00—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms
- C07C55/02—Dicarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C55/00—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms
- C07C55/22—Tricarboxylic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C55/00—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms
- C07C55/24—Saturated compounds having more than one carboxyl group bound to acyclic carbon atoms containing more than three carboxyl groups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K49/00—Preparations for testing in vivo
- A61K49/001—Preparation for luminescence or biological staining
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/908—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Inorganic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562159297P | 2015-05-10 | 2015-05-10 | |
| US62/159,297 | 2015-05-10 | ||
| PCT/EP2016/060263 WO2016180747A1 (en) | 2015-05-10 | 2016-05-09 | Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2984701A1 CA2984701A1 (en) | 2016-11-17 |
| CA2984701C true CA2984701C (en) | 2023-09-26 |
Family
ID=55953158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2984701A Active CA2984701C (en) | 2015-05-10 | 2016-05-09 | Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays |
Country Status (7)
| Country | Link |
|---|---|
| US (3) | US11162877B2 (https=) |
| EP (1) | EP3295173B1 (https=) |
| JP (2) | JP7044554B2 (https=) |
| CN (1) | CN107980095A (https=) |
| AU (1) | AU2016261279B2 (https=) |
| CA (1) | CA2984701C (https=) |
| WO (1) | WO2016180747A1 (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3853214A1 (en) | 2018-09-20 | 2021-07-28 | Ventana Medical Systems, Inc. | Coumarin-based crosslinking reagents |
| WO2020132857A1 (zh) | 2018-12-25 | 2020-07-02 | 杭州翰思生物医药有限公司 | 抗ox40的单克隆抗体及其应用 |
| JP2024504944A (ja) | 2021-01-15 | 2024-02-02 | ヴェンタナ メディカル システムズ， インク． | 保存安定なケージドハプテン |
| EP4281779A1 (en) | 2021-01-25 | 2023-11-29 | Ventana Medical Systems, Inc. | Stained biological specimens including one or more biomarkers labeled with one or more detectable moieties |
| US12555497B2 (en) | 2021-03-30 | 2026-02-17 | Sato Corporation | Label structure and label |
| WO2022223137A1 (en) | 2021-04-18 | 2022-10-27 | Ventana Medical Systems, Inc. | Morphological marker staining |
| CN113552362B (zh) * | 2021-07-23 | 2024-08-09 | 湖北百奥斯生物科技有限公司 | 一种新型信号放大的免疫荧光试剂盒 |
| CN120359417A (zh) | 2022-12-23 | 2025-07-22 | 文塔纳医疗系统公司 | 用于评估抗原呈递机制组分的材料和方法及其用途 |
| WO2025014787A1 (en) | 2023-07-07 | 2025-01-16 | Ventana Medical Systems, Inc. | Brightfield triplex immunohistochemistry assay for evaluating the colocalization of the er, pr, and ki-67 biomarkers in cells |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3410400C2 (de) * | 1984-03-21 | 1987-04-02 | Titmus Eurocon Kontaktlinsen GmbH, 8750 Aschaffenburg | Kontaktlinsenpflegesatz und Verfahren zur Pflege von Kontaktlinsen |
| US5595707A (en) | 1990-03-02 | 1997-01-21 | Ventana Medical Systems, Inc. | Automated biological reaction apparatus |
| US5225325A (en) * | 1990-03-02 | 1993-07-06 | Ventana Medical Systems, Inc. | Immunohistochemical staining method and reagents therefor |
| JP2000511291A (ja) * | 1997-10-21 | 2000-08-29 | キャンサー リサーチ キャンペーン テクノロジー リミティド | 細胞の増殖異常の測定 |
| ES2354598T3 (es) | 1998-02-27 | 2011-03-16 | Ventana Medical Systems, Inc. | Aparato de patología molecular automatizado que tiene calefactores de portaobjetos independientes. |
| US20030211630A1 (en) | 1998-02-27 | 2003-11-13 | Ventana Medical Systems, Inc. | Automated molecular pathology apparatus having independent slide heaters |
| US6582962B1 (en) | 1998-02-27 | 2003-06-24 | Ventana Medical Systems, Inc. | Automated molecular pathology apparatus having independent slide heaters |
| SE9804064D0 (sv) | 1998-11-25 | 1998-11-25 | A & Science Invest Ab | Medicinal product and method for treatment of conditions affecting neural stem cells or progenitor cells |
| US20050176072A1 (en) * | 2002-06-21 | 2005-08-11 | Martin Michelle D. | MTA1 is a predictive and prognostic factor in human breast cancer |
| US20040248223A1 (en) * | 2003-06-05 | 2004-12-09 | Idexx Laboratories, Inc. | Reduction of interfering peroxidase activity in peroxidase based detection methods |
| WO2005033333A2 (en) * | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
| JP5733734B2 (ja) * | 2009-02-19 | 2015-06-10 | 国立大学法人 千葉大学 | 成長因子に誘発されるユークロマチン化（ｅｕｃｈｒｏｍａｔｉｎｉｚａｔｉｏｎ）に必要な、ＳＲＣ−ファミリーチロシンキナーゼの核局在 |
| CA2762187C (en) | 2009-04-08 | 2017-08-01 | Olle Hernell | New methods for treatment of inflammatory diseases |
| CA2842661C (en) | 2009-10-19 | 2016-02-23 | Ventana Medical Systems, Inc. | Imaging system and techniques |
| WO2012164083A1 (en) * | 2011-06-01 | 2012-12-06 | Actogenix N.V. | Polycistronic expression system for bacteria |
| JP5898318B2 (ja) | 2011-09-09 | 2016-04-06 | ベンタナ メディカル システムズ， インコーポレイテッド | イメージングシステム、カセッテ、およびこれを使用する方法 |
| EP2831587B1 (en) | 2012-03-27 | 2018-05-16 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
-
2016
- 2016-05-09 AU AU2016261279A patent/AU2016261279B2/en active Active
- 2016-05-09 JP JP2017558986A patent/JP7044554B2/ja active Active
- 2016-05-09 EP EP16721425.3A patent/EP3295173B1/en active Active
- 2016-05-09 CN CN201680027053.XA patent/CN107980095A/zh active Pending
- 2016-05-09 WO PCT/EP2016/060263 patent/WO2016180747A1/en not_active Ceased
- 2016-05-09 CA CA2984701A patent/CA2984701C/en active Active
-
2017
- 2017-11-09 US US15/807,612 patent/US11162877B2/en active Active
-
2020
- 2020-12-10 JP JP2020205107A patent/JP2021058193A/ja active Pending
-
2021
- 2021-10-04 US US17/493,477 patent/US11971337B2/en active Active
-
2024
- 2024-03-30 US US18/622,914 patent/US20240328910A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2016261279A1 (en) | 2017-10-12 |
| EP3295173A1 (en) | 2018-03-21 |
| JP2021058193A (ja) | 2021-04-15 |
| US20220026319A1 (en) | 2022-01-27 |
| AU2016261279B2 (en) | 2020-12-10 |
| CA2984701A1 (en) | 2016-11-17 |
| US11971337B2 (en) | 2024-04-30 |
| US20180120202A1 (en) | 2018-05-03 |
| EP3295173B1 (en) | 2025-11-05 |
| JP7044554B2 (ja) | 2022-03-30 |
| US11162877B2 (en) | 2021-11-02 |
| CN107980095A (zh) | 2018-05-01 |
| JP2018520647A (ja) | 2018-08-02 |
| US20240328910A1 (en) | 2024-10-03 |
| WO2016180747A1 (en) | 2016-11-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20190430 |
|
| MPN | Maintenance fee for patent paid |
Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 9TH ANNIV.) - STANDARD Year of fee payment: 9 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250423 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20250925 |