CA2975027A1 - Means and methods for minimizing swept and dead volumes in chromatographic applications - Google Patents
Means and methods for minimizing swept and dead volumes in chromatographic applications Download PDFInfo
- Publication number
- CA2975027A1 CA2975027A1 CA2975027A CA2975027A CA2975027A1 CA 2975027 A1 CA2975027 A1 CA 2975027A1 CA 2975027 A CA2975027 A CA 2975027A CA 2975027 A CA2975027 A CA 2975027A CA 2975027 A1 CA2975027 A1 CA 2975027A1
- Authority
- CA
- Canada
- Prior art keywords
- column
- less
- fractions
- chromatographic
- chromatography step
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000011210 chromatographic step Methods 0.000 claims description 40
- 239000000463 material Substances 0.000 claims description 31
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 15
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 9
- DTQVDTLACAAQTR-UHFFFAOYSA-N Trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F DTQVDTLACAAQTR-UHFFFAOYSA-N 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 239000003607 modifier Substances 0.000 claims description 7
- 230000002378 acidificating effect Effects 0.000 claims description 4
- 238000005342 ion exchange Methods 0.000 claims description 4
- 230000029087 digestion Effects 0.000 claims description 3
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- 230000003993 interaction Effects 0.000 claims description 3
- 238000004949 mass spectrometry Methods 0.000 claims description 3
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- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 230000007935 neutral effect Effects 0.000 claims description 2
- 238000005194 fractionation Methods 0.000 description 31
- 150000001875 compounds Chemical class 0.000 description 13
- 230000001419 dependent effect Effects 0.000 description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
- 238000004811 liquid chromatography Methods 0.000 description 9
- 238000004587 chromatography analysis Methods 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 229910052739 hydrogen Inorganic materials 0.000 description 6
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000000470 constituent Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 4
- RWRDLPDLKQPQOW-UHFFFAOYSA-N Pyrrolidine Chemical compound C1CCNC1 RWRDLPDLKQPQOW-UHFFFAOYSA-N 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 239000012491 analyte Substances 0.000 description 3
- 239000011324 bead Substances 0.000 description 3
- 210000001124 body fluid Anatomy 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 230000001627 detrimental effect Effects 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 235000019253 formic acid Nutrition 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical class C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 1
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- HNSDLXPSAYFUHK-UHFFFAOYSA-N 1,4-bis(2-ethylhexyl) sulfosuccinate Chemical compound CCCCC(CC)COC(=O)CC(S(O)(=O)=O)C(=O)OCC(CC)CCCC HNSDLXPSAYFUHK-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 102100021935 C-C motif chemokine 26 Human genes 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 101000897493 Homo sapiens C-C motif chemokine 26 Proteins 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 108010026552 Proteome Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012504 chromatography matrix Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000002195 electrostatic repulsion hydrophilic interaction chromatography Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000002013 hydrophilic interaction chromatography Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/24—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the treatment of the fractions to be distributed
- B01D15/247—Fraction collectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/80—Fraction collectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N2030/6013—Construction of the column end pieces interfaces to detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Nanotechnology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15154374.1 | 2015-02-09 | ||
| EP15154374 | 2015-02-09 | ||
| PCT/EP2016/052490 WO2016128316A1 (en) | 2015-02-09 | 2016-02-05 | Means and methods for minimizing swept and dead volumes in chromatographic applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2975027A1 true CA2975027A1 (en) | 2016-08-18 |
Family
ID=52686078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2975027A Abandoned CA2975027A1 (en) | 2015-02-09 | 2016-02-05 | Means and methods for minimizing swept and dead volumes in chromatographic applications |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20180031528A1 (enExample) |
| EP (1) | EP3256846A1 (enExample) |
| JP (1) | JP2018508793A (enExample) |
| KR (1) | KR20170110716A (enExample) |
| CN (1) | CN107209155A (enExample) |
| AU (1) | AU2016218072A1 (enExample) |
| CA (1) | CA2975027A1 (enExample) |
| WO (1) | WO2016128316A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2018007204A (es) | 2015-12-16 | 2018-12-11 | Gritstone Oncology Inc | Identificacion, fabricacion y uso de neoantigeno. |
| AU2018348165B2 (en) | 2017-10-10 | 2025-09-04 | Seattle Project Corp. | Neoantigen identification using hotspots |
| US11885815B2 (en) | 2017-11-22 | 2024-01-30 | Gritstone Bio, Inc. | Reducing junction epitope presentation for neoantigens |
| US20190227040A1 (en) * | 2018-01-22 | 2019-07-25 | Thermo Finnigan Llc | Method and Apparatus for Chromatograph Nano-Flow Fractionator |
| CN112034083B (zh) * | 2020-07-30 | 2023-03-31 | 北京卫星制造厂有限公司 | 一种液相色谱泵流路超低死体积的标定方法及系统 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH037815Y2 (enExample) * | 1984-10-05 | 1991-02-26 | ||
| US5305788A (en) * | 1992-08-13 | 1994-04-26 | Whitey Co. | Stream selector for process analyzer |
| FR2809490B1 (fr) * | 2000-05-23 | 2002-11-29 | Hocer | Installation et procede pour la preparation automatique d'echantillons |
| US6748975B2 (en) * | 2001-12-26 | 2004-06-15 | Micralyne Inc. | Microfluidic valve and method of manufacturing same |
| WO2005114168A1 (en) * | 2004-05-22 | 2005-12-01 | Agilent Technologies, Inc. | Combi column |
| EP2491993B1 (en) * | 2005-01-20 | 2018-10-03 | Waters Technologies Corporation | Methods for separating compounds |
| CN2859526Y (zh) * | 2005-07-28 | 2007-01-17 | 上海烟草（集团）公司 | 一种大孔径柱到毛细柱的多维气相色谱系统 |
| US20070117729A1 (en) * | 2005-11-01 | 2007-05-24 | Taylor Rebecca A | Multi-phase personal care composition comprising a depositing perfume |
| WO2010072546A1 (en) * | 2008-12-04 | 2010-07-01 | Vrije Universiteit Brussel | A chromatographic separation device with variable length and a method for its use |
| US8656955B2 (en) * | 2010-05-20 | 2014-02-25 | Bio-Rad Laboratories, Inc. | Rotary column selector valve |
| EP2596134B1 (en) * | 2010-07-23 | 2020-04-08 | President and Fellows of Harvard College | Methods of detecting diseases or conditions using phagocytic cells |
| CN203935755U (zh) * | 2010-09-22 | 2014-11-12 | 魄金莱默保健科学有限公司 | 用于色谱的反冲系统和装置 |
| GB2504622B (en) * | 2011-05-26 | 2015-09-16 | Thermo Electron Mfg Ltd | Method and apparatus for improved resolution chromatography |
| GB2491169B (en) * | 2011-05-26 | 2014-09-10 | Thermo Electron Mfg Ltd | Method and apparatus for improved resolution chromatography |
| WO2012170756A1 (en) * | 2011-06-09 | 2012-12-13 | Waters Technologies Corporation | Reducing dispersion due to vias in planar microfluidic separation devices |
| HUP1100312A2 (en) * | 2011-06-14 | 2013-01-28 | Emil Dr Mincsovics | Procedure and equipment for improving of performance characteristics of column chromatography separation processes |
| CN203060889U (zh) * | 2012-12-19 | 2013-07-17 | 大连依利特分析仪器有限公司 | 液相色谱用全自动制备级馏分收集阀站 |
-
2016
- 2016-02-05 US US15/549,656 patent/US20180031528A1/en not_active Abandoned
- 2016-02-05 EP EP16704555.8A patent/EP3256846A1/en not_active Withdrawn
- 2016-02-05 WO PCT/EP2016/052490 patent/WO2016128316A1/en not_active Ceased
- 2016-02-05 KR KR1020177025025A patent/KR20170110716A/ko not_active Withdrawn
- 2016-02-05 AU AU2016218072A patent/AU2016218072A1/en not_active Abandoned
- 2016-02-05 JP JP2017559909A patent/JP2018508793A/ja active Pending
- 2016-02-05 CA CA2975027A patent/CA2975027A1/en not_active Abandoned
- 2016-02-05 CN CN201680009475.4A patent/CN107209155A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2018508793A (ja) | 2018-03-29 |
| WO2016128316A1 (en) | 2016-08-18 |
| KR20170110716A (ko) | 2017-10-11 |
| AU2016218072A1 (en) | 2017-08-24 |
| CN107209155A (zh) | 2017-09-26 |
| US20180031528A1 (en) | 2018-02-01 |
| EP3256846A1 (en) | 2017-12-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20210831 |
|
| FZDE | Discontinued |
Effective date: 20210831 |