CA2937832C - Methods for detecting multiple nucleic acids in a sample - Google Patents

Methods for detecting multiple nucleic acids in a sample Download PDF

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Publication number
CA2937832C
CA2937832C CA2937832A CA2937832A CA2937832C CA 2937832 C CA2937832 C CA 2937832C CA 2937832 A CA2937832 A CA 2937832A CA 2937832 A CA2937832 A CA 2937832A CA 2937832 C CA2937832 C CA 2937832C
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Canada
Prior art keywords
nucleic acid
amount
reporter
compound
binding member
Prior art date
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Active
Application number
CA2937832A
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English (en)
French (fr)
Other versions
CA2937832A1 (en
Inventor
Thomas Ullrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Rapid Diagnostics Jena GmbH
Original Assignee
Alere Technologies GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alere Technologies GmbH filed Critical Alere Technologies GmbH
Publication of CA2937832A1 publication Critical patent/CA2937832A1/en
Application granted granted Critical
Publication of CA2937832C publication Critical patent/CA2937832C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2937832A 2014-02-21 2015-02-23 Methods for detecting multiple nucleic acids in a sample Active CA2937832C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1403076.1A GB201403076D0 (en) 2014-02-21 2014-02-21 Methods for detecting multiple nucleic acids in a sample
GB1403076.1 2014-02-21
PCT/EP2015/053759 WO2015124779A1 (en) 2014-02-21 2015-02-23 Methods for detecting multiple nucleic acids in a sample

Publications (2)

Publication Number Publication Date
CA2937832A1 CA2937832A1 (en) 2015-08-27
CA2937832C true CA2937832C (en) 2023-10-03

Family

ID=50482582

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2937832A Active CA2937832C (en) 2014-02-21 2015-02-23 Methods for detecting multiple nucleic acids in a sample

Country Status (8)

Country Link
US (2) US10246737B2 (https=)
EP (3) EP3108008B1 (https=)
JP (2) JP6727137B2 (https=)
CN (1) CN106687598B (https=)
CA (1) CA2937832C (https=)
GB (1) GB201403076D0 (https=)
HK (1) HK1231512A1 (https=)
WO (1) WO2015124779A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN110672569A (zh) * 2019-09-30 2020-01-10 中国科学院宁波工业技术研究院慈溪生物医学工程研究所 一种dna或rna检测体系、检测方法和它们的应用
CN112553376B (zh) * 2020-12-23 2023-06-02 四川大学 引物组、试剂盒、区分不同ibv疫苗毒株的多重pcr检测方法及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
HK1046381A1 (zh) 1999-07-02 2003-01-10 Clondiag Chip Technologies Gmbh 用於复制和描述核酸的芯片矩阵装置
US6312929B1 (en) 2000-12-22 2001-11-06 Cepheid Compositions and methods enabling a totally internally controlled amplification reaction
CA2436518A1 (en) * 2001-01-30 2002-08-08 Vertex Pharmaceuticals Incorporated A quantitative assay for nucleic acids
CA2492423A1 (en) * 2001-07-15 2004-03-18 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
US7226732B2 (en) * 2001-07-16 2007-06-05 Cepheid Methods, apparatus, and computer programs for verifying the integrity of a probe
EP1493822A1 (de) 2003-07-02 2005-01-05 Labor Becker, Olgemoeller & Kollegen GbR Detektionsverfahren für Nucleinsäuren mit interner Amplifikationskontrolle
DE102004022263A1 (de) 2004-05-06 2005-12-15 Clondiag Chip Technologies Gmbh Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen
EP2061588B1 (en) 2006-11-06 2016-07-13 Clondiag GmbH Device and process for binding assays
EP2857526B1 (en) * 2006-12-13 2016-08-17 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
EP2620217B1 (en) 2007-05-03 2021-09-08 Abbott Rapid Diagnostics Jena GmbH Assay methods test microfluidic devices with flexible substrates
GB201403076D0 (en) 2014-02-21 2014-04-09 ALERE TECHNOLOGIES GmbH Methods for detecting multiple nucleic acids in a sample

Also Published As

Publication number Publication date
EP3800269A1 (en) 2021-04-07
JP2020114225A (ja) 2020-07-30
EP4400600A2 (en) 2024-07-17
GB201403076D0 (en) 2014-04-09
JP6727137B2 (ja) 2020-07-22
US20170081710A1 (en) 2017-03-23
JP2017506914A (ja) 2017-03-16
HK1231512A1 (zh) 2017-12-22
US10787700B2 (en) 2020-09-29
US20190376125A1 (en) 2019-12-12
EP3800269B1 (en) 2024-03-20
US10246737B2 (en) 2019-04-02
EP3108008A1 (en) 2016-12-28
EP4400600A3 (en) 2024-10-23
CN106687598A (zh) 2017-05-17
CN106687598B (zh) 2022-10-11
CA2937832A1 (en) 2015-08-27
WO2015124779A1 (en) 2015-08-27
EP3108008B1 (en) 2020-10-28

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