CA2935066C - Enhanced protein expression - Google Patents

Enhanced protein expression Download PDF

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Publication number
CA2935066C
CA2935066C CA2935066A CA2935066A CA2935066C CA 2935066 C CA2935066 C CA 2935066C CA 2935066 A CA2935066 A CA 2935066A CA 2935066 A CA2935066 A CA 2935066A CA 2935066 C CA2935066 C CA 2935066C
Authority
CA
Canada
Prior art keywords
protein
amino acid
gram positive
bacterial cell
positive bacterial
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2935066A
Other languages
English (en)
French (fr)
Other versions
CA2935066A1 (en
Inventor
Cristina Bongiorni
Brian F. Schmidt
Rei OTSUKA
Anita Van Kimmenade
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Danisco US Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CA2935066A1 publication Critical patent/CA2935066A1/en
Application granted granted Critical
Publication of CA2935066C publication Critical patent/CA2935066C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA2935066A 2013-12-31 2014-12-08 Enhanced protein expression Active CA2935066C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361922613P 2013-12-31 2013-12-31
US61/922,613 2013-12-31
PCT/US2014/069019 WO2015102814A1 (en) 2013-12-31 2014-12-08 Enhanced protein expression

Publications (2)

Publication Number Publication Date
CA2935066A1 CA2935066A1 (en) 2015-07-09
CA2935066C true CA2935066C (en) 2022-06-21

Family

ID=52273531

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2935066A Active CA2935066C (en) 2013-12-31 2014-12-08 Enhanced protein expression

Country Status (8)

Country Link
US (1) US10287591B2 (enExample)
EP (1) EP3089991B1 (enExample)
JP (1) JP6585602B2 (enExample)
CN (1) CN106068273B (enExample)
CA (1) CA2935066C (enExample)
DK (1) DK3089991T3 (enExample)
MX (1) MX2016008595A (enExample)
WO (1) WO2015102814A1 (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106599611B (zh) * 2016-12-09 2019-04-30 中南大学 蛋白质功能标注方法及系统

Family Cites Families (31)

* Cited by examiner, † Cited by third party
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US4302544A (en) 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US5310675A (en) 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
DE3480411D1 (en) 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5763257A (en) 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
EP0254735B2 (en) 1986-01-15 1998-06-17 Amgen Inc. METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
GB8608194D0 (en) 1986-04-03 1986-05-08 Massey Ferguson Services Nv Valve control system
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
PT86864B (pt) 1987-02-27 1992-05-29 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5482849A (en) 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
KR100258460B1 (ko) 1991-05-01 2000-06-01 한센 핀 베네드 안정화 효소 및 세제 조성물
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6617148B2 (en) * 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
ATE495245T1 (de) 2002-02-15 2011-01-15 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
BRPI0517908A (pt) * 2004-11-26 2008-10-14 Kyowa Hakko Kogyo Kk cepa mutante de e. coli, e, método para produzir uma substáncia útil
EP2099818A2 (en) * 2006-11-29 2009-09-16 Novozymes Inc. Bacillus licheniformis chromosome
US20100022861A1 (en) 2008-07-28 2010-01-28 Medtronic, Inc. Implantable optical hemodynamic sensor including an extension member
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina

Also Published As

Publication number Publication date
CA2935066A1 (en) 2015-07-09
EP3089991A1 (en) 2016-11-09
EP3089991B1 (en) 2019-08-28
JP2017500879A (ja) 2017-01-12
MX2016008595A (es) 2016-10-28
JP6585602B2 (ja) 2019-10-02
DK3089991T3 (da) 2019-12-02
CN106068273A (zh) 2016-11-02
US20160319288A1 (en) 2016-11-03
WO2015102814A1 (en) 2015-07-09
CN106068273B (zh) 2021-01-08
US10287591B2 (en) 2019-05-14

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