CA2924752C - Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein - Google Patents

Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein Download PDF

Info

Publication number
CA2924752C
CA2924752C CA2924752A CA2924752A CA2924752C CA 2924752 C CA2924752 C CA 2924752C CA 2924752 A CA2924752 A CA 2924752A CA 2924752 A CA2924752 A CA 2924752A CA 2924752 C CA2924752 C CA 2924752C
Authority
CA
Canada
Prior art keywords
protein
pore
polypeptide
peptide
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2924752A
Other languages
English (en)
French (fr)
Other versions
CA2924752A1 (en
Inventor
Hagan Bayley
David RODRIGUEZ-LARREA
Christian Bech ROSEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oxford University Innovation Ltd
Original Assignee
Oxford University Innovation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oxford University Innovation Ltd filed Critical Oxford University Innovation Ltd
Publication of CA2924752A1 publication Critical patent/CA2924752A1/en
Application granted granted Critical
Publication of CA2924752C publication Critical patent/CA2924752C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2924752A 2013-09-23 2014-09-22 Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein Active CA2924752C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1316849.7 2013-09-23
GBGB1316849.7A GB201316849D0 (en) 2013-09-23 2013-09-23 Method
US201361896933P 2013-10-29 2013-10-29
US61/896,933 2013-10-29
PCT/GB2014/052873 WO2015040423A1 (en) 2013-09-23 2014-09-22 Method

Publications (2)

Publication Number Publication Date
CA2924752A1 CA2924752A1 (en) 2015-03-26
CA2924752C true CA2924752C (en) 2022-11-29

Family

ID=49553252

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2924752A Active CA2924752C (en) 2013-09-23 2014-09-22 Method for determining the presence, absence, number or position(s) of one or more post-translational modifications (ptms) in a peptide, polypeptide or protein

Country Status (8)

Country Link
US (2) US10712254B2 (https=)
EP (1) EP3049814B1 (https=)
JP (1) JP6707453B2 (https=)
CN (1) CN105765387B (https=)
AU (1) AU2014322867B2 (https=)
CA (1) CA2924752C (https=)
GB (1) GB201316849D0 (https=)
WO (1) WO2015040423A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201614455D0 (en) 2016-08-24 2016-10-05 Univ Oxford Innovation Ltd Biomarkers
WO2020055246A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
CN109300501B (zh) * 2018-09-20 2021-02-02 国家卫生健康委科学技术研究所 蛋白质三维结构预测方法及用其构建的预测云平台
JP2022529623A (ja) * 2019-04-09 2022-06-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
CN112147185B (zh) * 2019-06-29 2022-07-01 清华大学 一种控制多肽穿过纳米孔速度的方法及其应用
EP4061965A1 (en) * 2019-11-19 2022-09-28 Rijksuniversiteit Groningen Artificial nanopores and uses and methods relating thereto
KR20220110213A (ko) * 2019-12-02 2022-08-05 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어를 사용하여 표적 폴리펩티드를 특징분석하는 방법
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux
EP4662489A1 (en) 2023-02-07 2025-12-17 Oxford University Innovation Limited Method of characterising a peptide, polypeptide or protein using a nanopore
WO2025003492A1 (en) 2023-06-30 2025-01-02 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) * 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
GB0820927D0 (en) * 2008-11-14 2008-12-24 Isis Innovation Method
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
WO2013116509A1 (en) 2012-02-01 2013-08-08 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
WO2013121201A1 (en) * 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
CA2864824C (en) * 2012-02-16 2020-06-16 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method

Also Published As

Publication number Publication date
US11592382B2 (en) 2023-02-28
GB201316849D0 (en) 2013-11-06
JP2016532125A (ja) 2016-10-13
EP3049814A1 (en) 2016-08-03
JP6707453B2 (ja) 2020-06-10
WO2015040423A1 (en) 2015-03-26
EP3049814B1 (en) 2021-03-17
CN105765387B (zh) 2019-10-18
CN105765387A (zh) 2016-07-13
AU2014322867B2 (en) 2020-10-08
CA2924752A1 (en) 2015-03-26
US20210096053A1 (en) 2021-04-01
US20160209317A1 (en) 2016-07-21
US10712254B2 (en) 2020-07-14
AU2014322867A1 (en) 2016-03-31

Similar Documents

Publication Publication Date Title
US11592382B2 (en) Method of analyzing post-translational modifications
US11739377B2 (en) Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US20250243472A1 (en) Modified helicases
US12371458B2 (en) Mutant pore
US20220145383A1 (en) Ssb method
AU2013220156B2 (en) Aptamer method
CN104126018B (zh) 酶方法
US20100297638A1 (en) Molecular adaptors
EP4709742A1 (en) Modified helicases

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190919

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 10TH ANNIV.) - STANDARD

Year of fee payment: 10

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20240910

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20240910

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20240910

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 11TH ANNIV.) - STANDARD

Year of fee payment: 11

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250916

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250916