CN105765387B - 用于确定在肽、多肽或蛋白质中翻译后修饰的方法 - Google Patents

用于确定在肽、多肽或蛋白质中翻译后修饰的方法 Download PDF

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CN105765387B
CN105765387B CN201480063812.9A CN201480063812A CN105765387B CN 105765387 B CN105765387 B CN 105765387B CN 201480063812 A CN201480063812 A CN 201480063812A CN 105765387 B CN105765387 B CN 105765387B
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protein
polypeptide
peptide
seq
duct
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CN105765387A (zh
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哈根·贝利
大卫·罗德里格斯-拉雷亚
克里斯蒂安·本奇·罗森
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Oxford Innovation Co Ltd, University of
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/10—Post-translational modifications [PTMs] in chemical analysis of biological material acylation, e.g. acetylation, formylation, lipoylation, myristoylation, palmitoylation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
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  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201480063812.9A 2013-09-23 2014-09-22 用于确定在肽、多肽或蛋白质中翻译后修饰的方法 Active CN105765387B (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB1316849.7 2013-09-23
GBGB1316849.7A GB201316849D0 (en) 2013-09-23 2013-09-23 Method
US201361896933P 2013-10-29 2013-10-29
US61/896,933 2013-10-29
PCT/GB2014/052873 WO2015040423A1 (en) 2013-09-23 2014-09-22 Method

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CN105765387A CN105765387A (zh) 2016-07-13
CN105765387B true CN105765387B (zh) 2019-10-18

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Country Link
US (2) US10712254B2 (https=)
EP (1) EP3049814B1 (https=)
JP (1) JP6707453B2 (https=)
CN (1) CN105765387B (https=)
AU (1) AU2014322867B2 (https=)
CA (1) CA2924752C (https=)
GB (1) GB201316849D0 (https=)
WO (1) WO2015040423A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
GB201614455D0 (en) 2016-08-24 2016-10-05 Univ Oxford Innovation Ltd Biomarkers
WO2020055246A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
CN109300501B (zh) * 2018-09-20 2021-02-02 国家卫生健康委科学技术研究所 蛋白质三维结构预测方法及用其构建的预测云平台
JP2022529623A (ja) * 2019-04-09 2022-06-23 オックスフォード ナノポール テクノロジーズ ピーエルシー 細孔
CN112147185B (zh) * 2019-06-29 2022-07-01 清华大学 一种控制多肽穿过纳米孔速度的方法及其应用
EP4061965A1 (en) * 2019-11-19 2022-09-28 Rijksuniversiteit Groningen Artificial nanopores and uses and methods relating thereto
KR20220110213A (ko) * 2019-12-02 2022-08-05 옥스포드 나노포어 테크놀로지즈 피엘씨 나노포어를 사용하여 표적 폴리펩티드를 특징분석하는 방법
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
IL320502A (en) 2022-10-28 2025-06-01 Univ Groningen Nanopore-based protein efflux
EP4662489A1 (en) 2023-02-07 2025-12-17 Oxford University Innovation Limited Method of characterising a peptide, polypeptide or protein using a nanopore
WO2025003492A1 (en) 2023-06-30 2025-01-02 Ecole Polytechnique Federale De Lausanne (Epfl) Uses of aerolysin nanopores

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010055307A1 (en) * 2008-11-14 2010-05-20 Isis Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
WO2013116509A1 (en) * 2012-02-01 2013-08-08 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
WO2013123379A2 (en) * 2012-02-16 2013-08-22 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
WO2013121201A1 (en) * 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US8278055B2 (en) * 2002-05-01 2012-10-02 Intel Corporation Methods and device for analyte characterization
US7744816B2 (en) * 2002-05-01 2010-06-29 Intel Corporation Methods and device for biomolecule characterization
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2008102120A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
WO2010004265A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Enzyme-pore constructs
JP2011527191A (ja) 2008-07-07 2011-10-27 オックスフォード ナノポア テクノロジーズ リミテッド 塩基検出細孔
US20120100530A1 (en) 2009-01-30 2012-04-26 Oxford Nanopore Technologies Limited Enzyme mutant
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
CN102741430B (zh) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 生化分析仪器、用于进行生化分析的第一模块以及相关方法
SG10201604316WA (en) * 2011-05-27 2016-07-28 Oxford Nanopore Tech Ltd Coupling method
NL2009191C2 (en) 2012-07-16 2014-01-20 Univ Delft Tech Single molecule protein sequencing.
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010055307A1 (en) * 2008-11-14 2010-05-20 Isis Innovation Limited Methods of enhancing translocation of charged analytes through transmembrane protein pores
WO2013116509A1 (en) * 2012-02-01 2013-08-08 Arizona Board Of Regents Acting For And On Behalf Of Arizona State University Systems, apparatuses and methods for reading an amino acid sequence
WO2013121201A1 (en) * 2012-02-15 2013-08-22 Oxford Nanopore Technologies Limited Aptamer method
WO2013123379A2 (en) * 2012-02-16 2013-08-22 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Multistep protein unfolding during nanopore translocation;avid Rodriguez-Larrea et al;《NATURE NANOTECHNOLOGY》;20130310;第8卷;摘要，图1 *
Single-molecule analysis of combinatorial epigenomic states in normal and tumor cells;Patrick J.Murphy et al;《PNAS》;20130507;第110卷(第9期);第7772-7777页 *
Single-Molecule Protein Unfolding in Solid State Nanopores;DavidS.Talaga et al;《JACS》;20091231;第131卷;第9287-9297页 *

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Publication number Publication date
US11592382B2 (en) 2023-02-28
GB201316849D0 (en) 2013-11-06
JP2016532125A (ja) 2016-10-13
EP3049814A1 (en) 2016-08-03
JP6707453B2 (ja) 2020-06-10
WO2015040423A1 (en) 2015-03-26
EP3049814B1 (en) 2021-03-17
CA2924752C (en) 2022-11-29
CN105765387A (zh) 2016-07-13
AU2014322867B2 (en) 2020-10-08
CA2924752A1 (en) 2015-03-26
US20210096053A1 (en) 2021-04-01
US20160209317A1 (en) 2016-07-21
US10712254B2 (en) 2020-07-14
AU2014322867A1 (en) 2016-03-31

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