CA2906818A1 - Generation de bibliotheques d'adn acellulaire directement a partir du sang - Google Patents

Generation de bibliotheques d'adn acellulaire directement a partir du sang

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Publication number
CA2906818A1
CA2906818A1 CA2906818A CA2906818A CA2906818A1 CA 2906818 A1 CA2906818 A1 CA 2906818A1 CA 2906818 A CA2906818 A CA 2906818A CA 2906818 A CA2906818 A CA 2906818A CA 2906818 A1 CA2906818 A1 CA 2906818A1
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Prior art keywords
cell
dna
sample
sequencing
free dna
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CA2906818A
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CA2906818C (fr
Inventor
Anupama Srinivasan
Richard P. Rava
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Verinata Health Inc
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Verinata Health Inc
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Priority to CA3156663A priority Critical patent/CA3156663A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)

Abstract

La présente invention concerne des procédés et trousses pour la préparation de bibliothèque de séquençage pour détecter une anomalie chromosomique au moyen d'ADN acellulaire (cfDNA) sans nécessiter un isolement préalable de l'ADN acellulaire depuis une fraction liquide d'un échantillon pour essai. Selon certains modes de réalisation, le procédé comprend la réduction de liaison entre l'ADN acellulaire et des protéines nucléosomiques sans déroulement de l'ADN acellulaire depuis les protéines nucléosomiques. Selon certains modes de réalisation, la réduction de liaison comprend également la congélation et la décongélation de l'échantillon pour essai avant la réduction de la liaison entre l'ADN acellulaire et les protéines nucléosomiques. Selon certains modes de réalisation, l'échantillon pour essai est un échantillon de sang périphérique prélevé d'une femme enceinte comportant l'ADN acellulaire de la maman et du ftus, les procédés pouvant être utilisés pour détecter une anomalie chromosomique ftale tel qu'une variation du nombre de copies. Selon d'autres modes de réalisation, l'échantillon pour essai est un échantillon de sang périphérique prélevé d'un patient connu ou suspecté d'être atteint d'un cancer, les procédés pouvant être utilisés pour détecter des anomalies chromosomiques dans l'ADN acellulaire du patient. L'invention concerne également des trousses pour la détection de variation du nombre de copies du ftus au moyen des procédés selon la présente invention.
CA2906818A 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang Active CA2906818C (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3156663A CA3156663A1 (fr) 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361801126P 2013-03-15 2013-03-15
US61/801,126 2013-03-15
PCT/US2014/029739 WO2014145078A1 (fr) 2013-03-15 2014-03-14 Génération de bibliothèques d'adn acellulaire directement à partir du sang

Related Child Applications (1)

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CA3156663A Division CA3156663A1 (fr) 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang

Publications (2)

Publication Number Publication Date
CA2906818A1 true CA2906818A1 (fr) 2014-09-18
CA2906818C CA2906818C (fr) 2022-08-16

Family

ID=50489439

Family Applications (2)

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CA3156663A Pending CA3156663A1 (fr) 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang
CA2906818A Active CA2906818C (fr) 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA3156663A Pending CA3156663A1 (fr) 2013-03-15 2014-03-14 Generation de bibliotheques d'adn acellulaire directement a partir du sang

Country Status (6)

Country Link
US (3) US10017807B2 (fr)
EP (2) EP3409791B1 (fr)
AU (2) AU2014233373B2 (fr)
CA (2) CA3156663A1 (fr)
HK (1) HK1218936A1 (fr)
WO (1) WO2014145078A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742058A (zh) * 2018-02-21 2020-10-02 纽克莱克斯有限公司 用于确定从全血分离血浆的效率的方法和试剂盒

Families Citing this family (168)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111544B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11332793B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
EP2854058A3 (fr) 2010-05-18 2015-10-28 Natera, Inc. Procédés pour une classification de ploïdie prénatale non invasive
US11408031B2 (en) 2010-05-18 2022-08-09 Natera, Inc. Methods for non-invasive prenatal paternity testing
US11326208B2 (en) 2010-05-18 2022-05-10 Natera, Inc. Methods for nested PCR amplification of cell-free DNA
US11332785B2 (en) 2010-05-18 2022-05-17 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US11339429B2 (en) 2010-05-18 2022-05-24 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US11261494B2 (en) 2012-06-21 2022-03-01 The Chinese University Of Hong Kong Method of measuring a fractional concentration of tumor DNA
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
JP6367196B2 (ja) 2012-08-14 2018-08-01 テンエックス・ジェノミクス・インコーポレイテッド マイクロカプセル組成物および方法
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
KR102028375B1 (ko) 2012-09-04 2019-10-04 가던트 헬쓰, 인크. 희귀 돌연변이 및 카피수 변이를 검출하기 위한 시스템 및 방법
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US9932576B2 (en) 2012-12-10 2018-04-03 Resolution Bioscience, Inc. Methods for targeted genomic analysis
EP3567116A1 (fr) 2012-12-14 2019-11-13 10X Genomics, Inc. Procédés et systèmes de traitement de polynucléotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9644204B2 (en) 2013-02-08 2017-05-09 10X Genomics, Inc. Partitioning and processing of analytes and other species
CA3156663A1 (fr) 2013-03-15 2014-09-18 Verinata Health, Inc. Generation de bibliotheques d'adn acellulaire directement a partir du sang
EP2999792B1 (fr) 2013-05-23 2018-11-14 The Board of Trustees of The Leland Stanford Junior University Transposition en une chromatine endogène permettant l'épigénomique personnelle
CA3189752A1 (fr) 2013-05-24 2014-11-27 Sequenom, Inc. Methodes et systemes d'evaluation non invasive de variations genetiques
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN105829589B (zh) 2013-11-07 2021-02-02 小利兰·斯坦福大学理事会 用于分析人体微生物组及其组分的无细胞核酸
WO2017062863A1 (fr) 2015-10-09 2017-04-13 Accuragen Holdings Limited Procédés et compositions pour enrichissement de produits d'amplification
KR102640585B1 (ko) 2013-12-11 2024-02-23 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
EP3378952B1 (fr) 2013-12-28 2020-02-05 Guardant Health, Inc. Procédés et systèmes de détection de variants génétiques
WO2015157567A1 (fr) 2014-04-10 2015-10-15 10X Genomics, Inc. Dispositifs fluidiques, systèmes et procédés permettant d'encapsuler et de séparer des réactifs, et leurs applications
AU2015249846B2 (en) 2014-04-21 2021-07-22 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
US10975371B2 (en) * 2014-04-29 2021-04-13 Illumina, Inc. Nucleic acid sequence analysis from single cells
KR102531677B1 (ko) 2014-06-26 2023-05-10 10엑스 제노믹스, 인크. 개별 세포 또는 세포 개체군으로부터 핵산을 분석하는 방법
PT3543356T (pt) 2014-07-18 2021-10-04 Univ Hong Kong Chinese Análise dos padrões de metilação de tecidos em mistura de adn
EP3175000B1 (fr) 2014-07-30 2020-07-29 Sequenom, Inc. Méthodes et procédés d'évaluation non invasive de variations génétiques
CN104293941B (zh) * 2014-09-30 2017-01-11 天津华大基因科技有限公司 构建测序文库的方法及其应用
CN104293940B (zh) * 2014-09-30 2017-07-28 天津华大基因科技有限公司 构建测序文库的方法及其应用
CN107111692B (zh) 2014-10-10 2021-10-29 生命科技股份有限公司 用于计算经校正扩增子覆盖度的方法、系统及计算机可读媒体
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
EP3218518B1 (fr) * 2014-11-12 2020-01-15 Neogenomics Laboratories, Inc. Détermination de la charge tumorale et d'une mutation bi-allélique chez les patients porteurs d'une mutation calr faisant appel au plasma sanguin périphérique
WO2016109939A1 (fr) * 2015-01-06 2016-07-14 深圳市海普洛斯生物科技有限公司 Procédé et réactif pour l'enrichissement de l'adn tumoral circulant
EP3244992B1 (fr) 2015-01-12 2023-03-08 10X Genomics, Inc. Procédés de codage a barres d'acides nucléiques
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
EP3256605B1 (fr) 2015-02-10 2022-02-09 The Chinese University Of Hong Kong Détection de mutations utilisées pour le dépistage du cancer et l'analyse foetale
EP4286516A3 (fr) 2015-02-24 2024-03-06 10X Genomics, Inc. Procédés et systèmes de traitement de cloisonnement
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
KR101967878B1 (ko) * 2015-03-12 2019-04-11 재단법인 아산사회복지재단 순환 유리 핵산의 분리 방법
JP6917629B2 (ja) 2015-04-29 2021-08-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 鎖特異的ｃＤＮＡライブラリーを構築するための組成物および方法
EP3294906B1 (fr) 2015-05-11 2024-07-10 Natera, Inc. Procédés pour la détermination de la ploïdie
AU2016264102A1 (en) * 2015-05-18 2017-12-21 Karius, Inc. Compositions and methods for enriching populations of nucleic acids
CN104894268B (zh) * 2015-06-05 2018-02-09 上海美吉生物医药科技有限公司 定量样本中源自细胞凋亡的dna浓度的方法及其应用
HUE064231T2 (hu) 2015-07-23 2024-02-28 Univ Hong Kong Chinese Sejtmentes DNS fragmentációs mintázatának elemzése
WO2017027835A1 (fr) * 2015-08-12 2017-02-16 Circulogene Theranostics, Llc Procédé de préparation de molécules d'acide nucléique sans cellule par amplification in situ
DK3374525T3 (da) 2015-11-11 2021-04-06 Resolution Bioscience Inc Højeffektiv konstruktion af dna-biblioteker
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
US20170145475A1 (en) * 2015-11-20 2017-05-25 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
DK3882357T3 (da) 2015-12-04 2022-08-29 10X Genomics Inc Fremgangsmåder og sammensætninger til analyse af nukleinsyrer
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
US20170175182A1 (en) * 2015-12-18 2017-06-22 Agilent Technologies, Inc. Transposase-mediated barcoding of fragmented dna
WO2017138984A1 (fr) 2016-02-11 2017-08-17 10X Genomics, Inc. Systèmes, procédés, et milieux destinés à l'assemblage de novo de données de séquence du génome entier
CA3185611A1 (fr) 2016-03-25 2017-09-28 Karius, Inc. Spike-ins d'acides nucleiques synthetiques
WO2017197338A1 (fr) 2016-05-13 2017-11-16 10X Genomics, Inc. Systèmes microfluidiques et procédés d'utilisation
EP3458586B1 (fr) 2016-05-16 2022-12-28 Accuragen Holdings Limited Procédé de séquençage amélioré par identification de brin
US10240196B2 (en) 2016-05-27 2019-03-26 Agilent Technologies, Inc. Transposase-random priming DNA sample preparation
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
AU2017295717B2 (en) 2016-07-15 2021-06-24 The Regents Of The University Of California Methods of producing nucleic acid libraries
WO2018018008A1 (fr) * 2016-07-22 2018-01-25 Oregon Health & Science University Bibliothèques de génome entier de cellules uniques et procédés d'indexage combinatoire pour leur fabrication
CN109844133A (zh) 2016-08-15 2019-06-04 安可济控股有限公司 检测罕见序列变体的组合物和方法
MX2019002093A (es) 2016-08-25 2019-06-20 Resolution Bioscience Inc Métodos para la detección de cambios de copia genómica en muestras de adn.
US11854666B2 (en) 2016-09-29 2023-12-26 Myriad Women's Health, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
WO2018067517A1 (fr) 2016-10-04 2018-04-12 Natera, Inc. Procédés pour caractériser une variation de nombre de copies à l'aide d'un séquençage de ligature de proximité
CN110191962A (zh) * 2016-10-21 2019-08-30 外来体诊断公司 外来体相关核酸的测序和分析
IL266654B2 (en) * 2016-11-17 2024-03-01 Lgc Clinical Diagnostics Inc Methods for preparing reference material for DNA and controls
IL266347B1 (en) 2016-11-30 2024-09-01 Univ Hong Kong Chinese Analysis of cell-free DNA in urine and other samples
US10011870B2 (en) 2016-12-07 2018-07-03 Natera, Inc. Compositions and methods for identifying nucleic acid molecules
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11694768B2 (en) 2017-01-24 2023-07-04 Sequenom, Inc. Methods and processes for assessment of genetic variations
TW202348802A (zh) 2017-01-25 2023-12-16 香港中文大學 使用核酸片段之診斷應用
WO2018140966A1 (fr) 2017-01-30 2018-08-02 10X Genomics, Inc. Procédés et systèmes de codage à barres de cellules individuelles sur la base de gouttelettes
WO2018144216A1 (fr) 2017-01-31 2018-08-09 Counsyl, Inc. Procédés et compositions pour l'enrichissement de polynucléotides cibles
US10752946B2 (en) 2017-01-31 2020-08-25 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
CA3049139A1 (fr) * 2017-02-21 2018-08-30 Natera, Inc. Compositions, procedes, et kits d'isolement d'acides nucleiques
WO2018160907A1 (fr) * 2017-03-03 2018-09-07 Counsyl, Inc. Extraction d'acides nucléiques pour une variabilité réduite du nombre de sondes
PT3596233T (pt) * 2017-03-17 2022-08-22 Sequenom Inc Métodos e processos para avaliação de mosaicismo genético
US11232850B2 (en) 2017-03-24 2022-01-25 Myriad Genetics, Inc. Copy number variant caller
EP3610034B1 (fr) 2017-04-12 2022-06-08 Karius, Inc. Systèmes, procédés et compositions de préparation d'échantillon
JP7079029B2 (ja) * 2017-04-17 2022-06-01 ジーンファースト リミテッド 核酸ライブラリーを調製するための方法、組成物、およびキット
JP7170711B2 (ja) * 2017-04-18 2022-11-14 アジレント・テクノロジーズ・ベルジャム・ナムローゼ・フェンノートシャップ Ｄｎａ分析のためのオフターゲット配列の使用
US10858691B2 (en) * 2017-04-18 2020-12-08 Covaris, Inc. Differential shearing of nucleic acids
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2019016289A1 (fr) * 2017-07-18 2019-01-24 Congenica Ltd. Système et procédé de dépistage
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (fr) 2017-10-26 2019-05-02 10X Genomics, Inc. Méthodes et systèmes de préparation d'acide nucléique et d'analyse de chromatine
WO2019084245A1 (fr) 2017-10-27 2019-05-02 Myriad Women's Health, Inc. Méthodes et compositions utilisables pour préparer des bibliothèques d'acides nucléiques
EP4241882A3 (fr) 2017-10-27 2023-12-06 10X Genomics, Inc. Procédés de préparation et d'analyse d'échantillons
KR20200085783A (ko) * 2017-10-27 2020-07-15 주노 다이어그노스틱스, 인크. 초저용량 액체 생검을 위한 장치, 시스템 및 방법
EP3625361A1 (fr) 2017-11-15 2020-03-25 10X Genomics, Inc. Perles de gel fonctionnalisées
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3719182B1 (fr) * 2017-11-27 2022-11-23 BGI Shenzhen Procédé de construction d'une bibliothèque d'adn acellulaire dans des liquides corporels et application associée
WO2019108851A1 (fr) 2017-11-30 2019-06-06 10X Genomics, Inc. Systèmes et procédés de préparation et d'analyse d'acides nucléiques
US12084720B2 (en) 2017-12-14 2024-09-10 Natera, Inc. Assessing graft suitability for transplantation
CN111712579B (zh) 2017-12-22 2024-10-15 10X基因组学有限公司 用于处理来自一个或多个细胞的核酸分子的系统和方法
EP3737774A1 (fr) 2018-01-12 2020-11-18 Claret Bioscience, LLC Procédés et compositions d'analyse d'acide nucléique
KR102036609B1 (ko) * 2018-02-12 2019-10-28 바이오코아 주식회사 디지털 pcr을 이용한 산전진단 방법
WO2019157529A1 (fr) 2018-02-12 2019-08-15 10X Genomics, Inc. Procédés de caractérisation d'analytes multiples à partir de cellules individuelles ou de populations cellulaires
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (fr) 2018-02-28 2019-09-06 10X Genomics, Inc. Séquençage de transcriptomes par ligation aléatoire
GB201819134D0 (en) * 2018-11-23 2019-01-09 Cancer Research Tech Ltd Improvements in variant detection
EP3762513A1 (fr) * 2018-03-08 2021-01-13 St. Johns University Biomarqueurs d'adn exempts de cellules sériques circulantes et procédés
WO2019178157A1 (fr) 2018-03-16 2019-09-19 Karius, Inc. Série d'échantillons pour différencier des acides nucléiques cibles d'acides nucléiques contaminants
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
AU2019248635B2 (en) * 2018-04-02 2022-01-27 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
CN112236520A (zh) 2018-04-02 2021-01-15 格里尔公司 甲基化标记和标靶甲基化探针板
WO2019195166A1 (fr) 2018-04-06 2019-10-10 10X Genomics, Inc. Systèmes et procédés de contrôle de qualité dans un traitement de cellules uniques
CA3090426A1 (fr) 2018-04-14 2019-10-17 Natera, Inc. Procedes de detection et de surveillance du cancer au moyen d'une detection personnalisee d'adn tumoral circulant
WO2019217758A1 (fr) 2018-05-10 2019-11-14 10X Genomics, Inc. Procédés et systèmes de génération de banque moléculaire
CA3067435C (fr) 2018-05-17 2023-09-12 Illumina, Inc. Sequencage de cellules uniques a haut debit avec biais d'amplification reduit
CA3100983A1 (fr) 2018-06-06 2019-12-12 The Regents Of The University Of California Procedes de production de bibliotheques d'acides nucleiques et compositions et kits pour leur mise en ƒuvre
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
CN112601823A (zh) 2018-06-12 2021-04-02 安可济控股有限公司 用于形成连接产物的方法和组合物
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US11525159B2 (en) 2018-07-03 2022-12-13 Natera, Inc. Methods for detection of donor-derived cell-free DNA
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
EP3856903A4 (fr) 2018-09-27 2022-07-27 Grail, LLC Marqueurs de méthylation et panels de sondes de méthylation ciblées
EP3884065B1 (fr) * 2018-11-21 2024-08-14 Karius, Inc. Procédés, systèmes et compositions de bibliothèque directe
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
AU2019403269A1 (en) 2018-12-18 2021-06-17 Grail, Llc Methods for detecting disease using analysis of RNA
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
EP3924505A1 (fr) 2019-02-12 2021-12-22 10X Genomics, Inc. Procédés de traitement de molécules d'acides nucléiques
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
US11931674B2 (en) * 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
US20220154249A1 (en) * 2019-04-15 2022-05-19 Natera, Inc. Improved liquid biopsy using size selection
CN110468180A (zh) * 2019-08-29 2019-11-19 北京优迅医学检验实验室有限公司 血浆dna文库及其构建方法
EP4087942A4 (fr) * 2020-01-08 2024-01-24 The Chinese University Of Hong Kong Types de fragments d'adn biterminal dans des échantillons acellulaires et leurs utilisations
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022182682A1 (fr) 2021-02-23 2022-09-01 10X Genomics, Inc. Analyse à base de sonde d'acides nucléiques et de protéines
EP4413580A1 (fr) * 2021-10-05 2024-08-14 Personalis, Inc. Essais personnalisés pour la surveillance personnalisée d'un cancer
CN113913333B (zh) * 2021-10-20 2022-09-02 南京世和基因生物技术股份有限公司 一种肺癌诊断标志物及用途
WO2024040957A1 (fr) * 2022-08-22 2024-02-29 Yunnan Daoran Science And Technology Ltd. Procédé d'analyse simplifié d'adn et d'adn acellulaire et ses utilisations
WO2024182805A1 (fr) * 2023-03-02 2024-09-06 Grail, Llc Rédaction d'adn acellulaire à partir d'échantillons de test pour classification par modèle de mélange

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0477220T3 (da) * 1989-05-22 1996-10-21 Hoffmann La Roche Fremgangsmåde til mærkning og sporing af materialer med nukleinsyrer
US6673621B1 (en) * 1996-10-04 2004-01-06 Intronn Llc Sample collection devices and methods using markers and the use of such markers as controls in sample validation, laboratory evaluation and/or accreditation
US20080138798A1 (en) * 2003-12-23 2008-06-12 Greg Hampikian Reference markers for biological samples
AU2006251937A1 (en) * 2005-05-27 2006-11-30 John Wayne Cancer Institute Use of free circulating DNA for diagnosis, prognosis, and treatment of cancer
CA2668818C (fr) 2006-10-10 2018-06-26 Xenomics, Inc. Compositions, procedes et trousses pour isoler acides nucleiques a partir de fluides corporels au moyen d'un milieu d'echange anionique
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US20100331195A1 (en) 2007-10-04 2010-12-30 William Andregg Sequencing Nucleic Acid Polymers with Electron Microscopy
US20110104725A1 (en) 2008-05-02 2011-05-05 Advanced Liquid Logic, Inc. Method of Effecting Coagulation in a Droplet
US8173401B2 (en) * 2008-06-30 2012-05-08 Life Technologies Coporation Method for direct amplification from crude nucleic acid samples
EP3378951B1 (fr) * 2008-09-20 2020-05-13 The Board of Trustees of the Leland Stanford Junior University Diagnostic non invasif d'une aneuploïdie par séquençage
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US11634747B2 (en) 2009-01-21 2023-04-25 Streck Llc Preservation of fetal nucleic acids in maternal plasma
EP2398912B1 (fr) 2009-02-18 2017-09-13 Streck Inc. Conservation des acides nucléiques extracellulaires
US20120135874A1 (en) * 2009-05-08 2012-05-31 The Johns Hopkins University Single molecule spectroscopy for analysis of cell-free nucleic acid biomarkers
CA2690651C (fr) * 2009-07-22 2016-10-04 Streck, Inc. Preservation des acides nucleiques foetaux dans le plasma maternel
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
US20120270739A1 (en) 2010-01-19 2012-10-25 Verinata Health, Inc. Method for sample analysis of aneuploidies in maternal samples
US9260745B2 (en) * 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
EP2366031B1 (fr) * 2010-01-19 2015-01-21 Verinata Health, Inc Méthodes de séquençage pour le diagnostic prénatal
HUE027972T2 (en) 2010-02-25 2016-11-28 Advanced Liquid Logic Inc A method for generating nucleic acid libraries
US8603742B2 (en) * 2010-07-06 2013-12-10 University of Pittsburgh—of the Commonwealth System of Higher Education Methods for the diagnosis of fetal disease
DE202013012535U1 (de) * 2012-02-13 2017-08-28 Streck Inc. Blutsammelvorrichtung für verbesserte Nukleinsäureregulierung
WO2013192570A1 (fr) * 2012-06-21 2013-12-27 Gigagen, Inc. Système et procédés d'analyse génétique de populations cellulaires mixtes
CA2878246C (fr) 2012-07-20 2022-01-11 Verinata Health, Inc. Detection et classification de variation du nombre de copies dans un genome de cancer
CA3156663A1 (fr) 2013-03-15 2014-09-18 Verinata Health, Inc. Generation de bibliotheques d'adn acellulaire directement a partir du sang

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111742058A (zh) * 2018-02-21 2020-10-02 纽克莱克斯有限公司 用于确定从全血分离血浆的效率的方法和试剂盒

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