CA2906278C - Isocitrate deshydrogenase de type procaryote et son application pour l'amelioration de l'utilisation d'azote dans des plantes transgeniques - Google Patents

Isocitrate deshydrogenase de type procaryote et son application pour l'amelioration de l'utilisation d'azote dans des plantes transgeniques Download PDF

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Publication number
CA2906278C
CA2906278C CA2906278A CA2906278A CA2906278C CA 2906278 C CA2906278 C CA 2906278C CA 2906278 A CA2906278 A CA 2906278A CA 2906278 A CA2906278 A CA 2906278A CA 2906278 C CA2906278 C CA 2906278C
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Prior art keywords
plant
nucleotide sequence
sequence
nitrogen
icdh
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Expired - Fee Related
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CA2906278A
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CA2906278A1 (fr
Inventor
James Mclaren
Brian Vande Berg
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Iowa Corn Promotion Board
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Iowa Corn Promotion Board
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La présente invention concerne des plantes transgéniques qui ont une efficacité d'utilisation d'azote améliorée, une tolérance au stress améliorée et/ou la diminution d'une limitation de telle sorte que le rendement soit accru, ou une combinaison de ceux-ci, et qui ont été transformées à l'aide d'une nouvelle construction vectorielle comprenant un gène d'isocitrate déshydrogénase (icdh) synthétique gène qui module l'utilisation d'azote dans des plantes. L'invention concerne également l'empilement du gène icdh avec d'autres gènes exogènes ou hétérologues qui modulent l'utilisation d'azote dans la plante, comprenant un gène de N-acétylglutamate kinase. L'invention concerne également des procédés d'expression dans des plantes des molécules d'acide nucléique correspondant aux séquences d'acide nucléique qui modulent l'utilisation d'azote dans des plantes ou qui sont modulées par des conditions d'azote.
CA2906278A 2013-03-15 2013-03-15 Isocitrate deshydrogenase de type procaryote et son application pour l'amelioration de l'utilisation d'azote dans des plantes transgeniques Expired - Fee Related CA2906278C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2013/031913 WO2014142946A1 (fr) 2013-03-15 2013-03-15 Isocitrate déshydrogénase de type procaryote et son application pour l'amélioration de l'utilisation d'azote dans des plantes transgéniques

Publications (2)

Publication Number Publication Date
CA2906278A1 CA2906278A1 (fr) 2014-09-18
CA2906278C true CA2906278C (fr) 2020-03-10

Family

ID=51537318

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2906278A Expired - Fee Related CA2906278C (fr) 2013-03-15 2013-03-15 Isocitrate deshydrogenase de type procaryote et son application pour l'amelioration de l'utilisation d'azote dans des plantes transgeniques

Country Status (9)

Country Link
EP (1) EP2966987A4 (fr)
CN (1) CN105338806A (fr)
AU (1) AU2013381961A1 (fr)
BR (1) BR112015023546A2 (fr)
CA (1) CA2906278C (fr)
MX (1) MX360004B (fr)
PH (1) PH12015502140A1 (fr)
WO (1) WO2014142946A1 (fr)
ZA (1) ZA201602825B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107557368A (zh) * 2016-06-30 2018-01-09 未名生物农业集团有限公司 非生物胁迫耐性提高的植物和方法
CN113502276B (zh) * 2021-08-03 2022-07-15 昆明理工大学 异柠檬酸脱氢酶在提高植物甲醛吸收代谢能力中的应用
CN116478956B (zh) * 2023-06-14 2023-09-22 中国农业大学 棉花N-乙酰谷氨酸激酶GhNAGK、其编码基因及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1463299A (en) * 1997-12-02 1999-06-16 E.I. Du Pont De Nemours And Company Branched chain amino acid biosynthetic enzymes
US7589257B2 (en) * 2006-02-09 2009-09-15 Pioneer Hi-Bred International Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
US8692070B2 (en) * 2009-11-04 2014-04-08 Iowa Corn Promotion Board Plants with improved nitrogen utilization and stress tolerance

Also Published As

Publication number Publication date
EP2966987A1 (fr) 2016-01-20
EP2966987A4 (fr) 2017-01-04
MX360004B (es) 2018-10-18
MX2015012951A (es) 2016-07-20
CA2906278A1 (fr) 2014-09-18
WO2014142946A1 (fr) 2014-09-18
PH12015502140A1 (en) 2016-01-25
AU2013381961A1 (en) 2015-10-01
ZA201602825B (en) 2019-06-26
CN105338806A (zh) 2016-02-17
BR112015023546A2 (pt) 2018-02-20

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EEER Examination request

Effective date: 20171115

MKLA Lapsed

Effective date: 20220315