PH12015502140A1 - A prokaryotic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants - Google Patents

A prokaryotic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants

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Publication number
PH12015502140A1
PH12015502140A1 PH12015502140A PH12015502140A PH12015502140A1 PH 12015502140 A1 PH12015502140 A1 PH 12015502140A1 PH 12015502140 A PH12015502140 A PH 12015502140A PH 12015502140 A PH12015502140 A PH 12015502140A PH 12015502140 A1 PH12015502140 A1 PH 12015502140A1
Authority
PH
Philippines
Prior art keywords
plants
nitrogen use
transgenic plants
isocitrate dehydrogenase
prokaryotic
Prior art date
Application number
PH12015502140A
Inventor
Mclaren James
Vande Berg Brian
Original Assignee
Ioewa Corn Promotion Board
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ioewa Corn Promotion Board filed Critical Ioewa Corn Promotion Board
Publication of PH12015502140A1 publication Critical patent/PH12015502140A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic isocitrate dehydrogenase ( icdh) gene that modulates nitrogen use in plants. The invention also relates to stacking the icdh gene with other exogenous or heterologous genes that modulate nitrogen use in the plant, including a N-acetylglutamate kinase gene. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
PH12015502140A 2013-03-15 2015-09-15 A prokaryotic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants PH12015502140A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2013/031913 WO2014142946A1 (en) 2013-03-15 2013-03-15 A prokaryotic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants

Publications (1)

Publication Number Publication Date
PH12015502140A1 true PH12015502140A1 (en) 2016-01-25

Family

ID=51537318

Family Applications (1)

Application Number Title Priority Date Filing Date
PH12015502140A PH12015502140A1 (en) 2013-03-15 2015-09-15 A prokaryotic-type isocitrate dehydrogenase and its application for improving nitrogen utilization in transgenic plants

Country Status (9)

Country Link
EP (1) EP2966987A4 (en)
CN (1) CN105338806A (en)
AU (1) AU2013381961A1 (en)
BR (1) BR112015023546A2 (en)
CA (1) CA2906278C (en)
MX (1) MX360004B (en)
PH (1) PH12015502140A1 (en)
WO (1) WO2014142946A1 (en)
ZA (1) ZA201602825B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107557368A (en) * 2016-06-30 2018-01-09 未名生物农业集团有限公司 The plant and method that abiotic stress tolerance improves
CN113502276B (en) * 2021-08-03 2022-07-15 昆明理工大学 Application of isocitrate dehydrogenase in improving formaldehyde absorption and metabolism capacity of plants
CN116478956B (en) * 2023-06-14 2023-09-22 中国农业大学 Cotton N-acetylglutamate kinase GhNAGK, coding gene and application thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1463299A (en) * 1997-12-02 1999-06-16 E.I. Du Pont De Nemours And Company Branched chain amino acid biosynthetic enzymes
EP2730587A3 (en) * 2006-02-09 2014-08-27 Pioneer Hi-Bred International, Inc. Genes for enhancing nitrogen utilization efficiency in crop plants
US8692070B2 (en) * 2009-11-04 2014-04-08 Iowa Corn Promotion Board Plants with improved nitrogen utilization and stress tolerance

Also Published As

Publication number Publication date
CN105338806A (en) 2016-02-17
MX2015012951A (en) 2016-07-20
EP2966987A1 (en) 2016-01-20
AU2013381961A1 (en) 2015-10-01
CA2906278A1 (en) 2014-09-18
ZA201602825B (en) 2019-06-26
EP2966987A4 (en) 2017-01-04
CA2906278C (en) 2020-03-10
BR112015023546A2 (en) 2018-02-20
WO2014142946A1 (en) 2014-09-18
MX360004B (en) 2018-10-18

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