CA2901021A1 - Cow rumen xylose isomerases active in yeast cells - Google Patents
Cow rumen xylose isomerases active in yeast cells Download PDFInfo
- Publication number
- CA2901021A1 CA2901021A1 CA2901021A CA2901021A CA2901021A1 CA 2901021 A1 CA2901021 A1 CA 2901021A1 CA 2901021 A CA2901021 A CA 2901021A CA 2901021 A CA2901021 A CA 2901021A CA 2901021 A1 CA2901021 A1 CA 2901021A1
- Authority
- CA
- Canada
- Prior art keywords
- xylose
- seq
- sequence
- yeast
- yeast cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108700040099 Xylose isomerases Proteins 0.000 title claims abstract description 88
- 210000005253 yeast cell Anatomy 0.000 title claims abstract description 58
- 210000004767 rumen Anatomy 0.000 title abstract description 6
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims abstract description 116
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims abstract description 61
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims abstract description 60
- 230000000694 effects Effects 0.000 claims abstract description 48
- 229920001184 polypeptide Polymers 0.000 claims abstract description 28
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 28
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 28
- 230000037361 pathway Effects 0.000 claims abstract description 26
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 76
- 108090000623 proteins and genes Proteins 0.000 claims description 71
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 37
- 150000007523 nucleic acids Chemical class 0.000 claims description 36
- 108020004707 nucleic acids Proteins 0.000 claims description 30
- 102000039446 nucleic acids Human genes 0.000 claims description 30
- 238000004519 manufacturing process Methods 0.000 claims description 18
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 17
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 14
- 229910052799 carbon Inorganic materials 0.000 claims description 14
- 150000001875 compounds Chemical class 0.000 claims description 13
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 claims description 7
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims description 7
- 229920000166 polytrimethylene carbonate Polymers 0.000 claims description 7
- 230000037353 metabolic pathway Effects 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 47
- 239000002028 Biomass Substances 0.000 abstract description 20
- 239000000413 hydrolysate Substances 0.000 abstract description 5
- 238000000855 fermentation Methods 0.000 abstract description 4
- 230000004151 fermentation Effects 0.000 abstract description 4
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 46
- 230000014509 gene expression Effects 0.000 description 37
- 108091026890 Coding region Proteins 0.000 description 33
- 239000013598 vector Substances 0.000 description 32
- 101150011516 xlnD gene Proteins 0.000 description 30
- 101150052264 xylA gene Proteins 0.000 description 30
- 210000004027 cell Anatomy 0.000 description 24
- 239000002773 nucleotide Substances 0.000 description 17
- 125000003729 nucleotide group Chemical group 0.000 description 17
- 239000002609 medium Substances 0.000 description 16
- 102000004169 proteins and genes Human genes 0.000 description 16
- 108020004414 DNA Proteins 0.000 description 15
- 239000000203 mixture Substances 0.000 description 14
- 230000010354 integration Effects 0.000 description 13
- 102000004190 Enzymes Human genes 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 12
- 239000003550 marker Substances 0.000 description 12
- 235000000346 sugar Nutrition 0.000 description 12
- 108020004705 Codon Proteins 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- 238000012217 deletion Methods 0.000 description 9
- 230000037430 deletion Effects 0.000 description 9
- 239000013612 plasmid Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 9
- 150000008163 sugars Chemical class 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 108091028043 Nucleic acid sequence Proteins 0.000 description 6
- 108060007030 Ribulose-phosphate 3-epimerase Proteins 0.000 description 6
- 101100069420 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GRE3 gene Proteins 0.000 description 6
- 101710094544 Transketolase 1 Proteins 0.000 description 6
- 102100029089 Xylulose kinase Human genes 0.000 description 6
- 240000008042 Zea mays Species 0.000 description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 6
- 235000005822 corn Nutrition 0.000 description 6
- 150000002972 pentoses Chemical class 0.000 description 6
- 230000001105 regulatory effect Effects 0.000 description 6
- 102000004688 ribulosephosphate 3-epimerase Human genes 0.000 description 6
- 238000011144 upstream manufacturing Methods 0.000 description 6
- 108091022915 xylulokinase Proteins 0.000 description 6
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 238000010369 molecular cloning Methods 0.000 description 5
- 108700010070 Codon Usage Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 241001570530 Ruminococcus champanellensis 18P13 = JCM 17042 Species 0.000 description 4
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 230000006801 homologous recombination Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000010907 stover Substances 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 241000201856 Abiotrophia defectiva Species 0.000 description 3
- KTVPXOYAKDPRHY-SOOFDHNKSA-N D-ribofuranose 5-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O KTVPXOYAKDPRHY-SOOFDHNKSA-N 0.000 description 3
- ZAQJHHRNXZUBTE-WUJLRWPWSA-N D-xylulose Chemical compound OC[C@@H](O)[C@H](O)C(=O)CO ZAQJHHRNXZUBTE-WUJLRWPWSA-N 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 229920002488 Hemicellulose Polymers 0.000 description 3
- 241000192026 Ruminococcus flavefaciens Species 0.000 description 3
- 241001474297 Ruminococcus flavefaciens FD-1 Species 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 108020004530 Transaldolase Proteins 0.000 description 3
- 102100028601 Transaldolase Human genes 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- BTANRVKWQNVYAZ-UHFFFAOYSA-N butan-2-ol Chemical compound CCC(C)O BTANRVKWQNVYAZ-UHFFFAOYSA-N 0.000 description 3
- 238000004422 calculation algorithm Methods 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000012239 gene modification Methods 0.000 description 3
- 230000005017 genetic modification Effects 0.000 description 3
- 235000013617 genetically modified food Nutrition 0.000 description 3
- 229920005610 lignin Polymers 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 230000008439 repair process Effects 0.000 description 3
- 239000013605 shuttle vector Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 229940035893 uracil Drugs 0.000 description 3
- 108010004277 2-acetolactate mutase Proteins 0.000 description 2
- 241000609240 Ambelania acida Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 101100434663 Bacillus subtilis (strain 168) fbaA gene Proteins 0.000 description 2
- 108010051219 Cre recombinase Proteins 0.000 description 2
- 101150095274 FBA1 gene Proteins 0.000 description 2
- 101150094690 GAL1 gene Proteins 0.000 description 2
- 102100028501 Galanin peptides Human genes 0.000 description 2
- 101100121078 Homo sapiens GAL gene Proteins 0.000 description 2
- 101000579123 Homo sapiens Phosphoglycerate kinase 1 Proteins 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- KJWZYMMLVHIVSU-IYCNHOCDSA-N PGK1 Chemical compound CCCCC[C@H](O)\C=C\[C@@H]1[C@@H](CCCCCCC(O)=O)C(=O)CC1=O KJWZYMMLVHIVSU-IYCNHOCDSA-N 0.000 description 2
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 description 2
- 241000235648 Pichia Species 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 108020005091 Replication Origin Proteins 0.000 description 2
- 240000006394 Sorghum bicolor Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- 239000010905 bagasse Substances 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 239000012978 lignocellulosic material Substances 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920001542 oligosaccharide Polymers 0.000 description 2
- 150000002482 oligosaccharides Chemical class 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 108091033319 polynucleotide Proteins 0.000 description 2
- 102000040430 polynucleotide Human genes 0.000 description 2
- 239000002157 polynucleotide Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 231100000241 scar Toxicity 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 241001300301 uncultured bacterium Species 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
- PKAUICCNAWQPAU-UHFFFAOYSA-N 2-(4-chloro-2-methylphenoxy)acetic acid;n-methylmethanamine Chemical compound CNC.CC1=CC(Cl)=CC=C1OCC(O)=O PKAUICCNAWQPAU-UHFFFAOYSA-N 0.000 description 1
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 1
- 102000016912 Aldehyde Reductase Human genes 0.000 description 1
- 108010053754 Aldehyde reductase Proteins 0.000 description 1
- 235000016068 Berberis vulgaris Nutrition 0.000 description 1
- 241000335053 Beta vulgaris Species 0.000 description 1
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 description 1
- 101100480861 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) tdh gene Proteins 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 101100447466 Candida albicans (strain WO-1) TDH1 gene Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 101150038242 GAL10 gene Proteins 0.000 description 1
- 102100024637 Galectin-10 Human genes 0.000 description 1
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 1
- 102100036669 Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Human genes 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001149669 Hanseniaspora Species 0.000 description 1
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 1
- 101001072574 Homo sapiens Glycerol-3-phosphate dehydrogenase [NAD(+)], cytoplasmic Proteins 0.000 description 1
- 101000891113 Homo sapiens T-cell acute lymphocytic leukemia protein 1 Proteins 0.000 description 1
- 101000801742 Homo sapiens Triosephosphate isomerase Proteins 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000721708 Mastotermes darwiniensis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 101100285000 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) his-3 gene Proteins 0.000 description 1
- 241001520808 Panicum virgatum Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000081757 Phalaris arundinacea Species 0.000 description 1
- 241000193632 Piromyces sp. Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000866500 Reticulitermes speratus Species 0.000 description 1
- 241000192031 Ruminococcus Species 0.000 description 1
- 101000847169 Ruminococcus flavefaciens Xylose isomerase Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 101100352598 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GPM1 gene Proteins 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 101000702553 Schistosoma mansoni Antigen Sm21.7 Proteins 0.000 description 1
- 101000714192 Schistosoma mansoni Tegument antigen Proteins 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000746413 Spartina Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 102100040365 T-cell acute lymphocytic leukemia protein 1 Human genes 0.000 description 1
- 102100033598 Triosephosphate isomerase Human genes 0.000 description 1
- 241000235013 Yarrowia Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 101150073130 ampR gene Proteins 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000006481 glucose medium Substances 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000002029 lignocellulosic biomass Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000010813 municipal solid waste Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 108091027963 non-coding RNA Proteins 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 239000010893 paper waste Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000010908 plant waste Substances 0.000 description 1
- 230000019525 primary metabolic process Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000001448 refractive index detection Methods 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 101150088047 tdh3 gene Proteins 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000003827 upregulation Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000010925 yard waste Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000007222 ypd medium Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
- C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y503/00—Intramolecular oxidoreductases (5.3)
- C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
- C12Y503/01005—Xylose isomerase (5.3.1.5)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/792,308 | 2013-03-11 | ||
| US13/792,308 US8669076B1 (en) | 2013-03-11 | 2013-03-11 | Cow rumen xylose isomerases active in yeast cells |
| PCT/US2014/022267 WO2014164392A1 (en) | 2013-03-11 | 2014-03-10 | Cow rumen xylose isomerases active in yeast cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2901021A1 true CA2901021A1 (en) | 2014-10-09 |
Family
ID=50192724
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2901021A Abandoned CA2901021A1 (en) | 2013-03-11 | 2014-03-10 | Cow rumen xylose isomerases active in yeast cells |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8669076B1 (enExample) |
| EP (1) | EP2970936B1 (enExample) |
| JP (1) | JP6422478B2 (enExample) |
| CN (1) | CN105026559A (enExample) |
| AU (2) | AU2014249370A1 (enExample) |
| BR (1) | BR112015020155A2 (enExample) |
| CA (1) | CA2901021A1 (enExample) |
| MY (1) | MY168287A (enExample) |
| WO (1) | WO2014164392A1 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR102014027233A2 (pt) * | 2014-10-30 | 2016-05-24 | Biocelere Agroindustrial Ltda | cassete de expressão, micro-organismo geneticamente modificado para expressão de xilose isomerase, processo para produção de biocombustíveis e/ou bioquímicos e biocombustível e/ou bioquímicos produzidos |
| SI3026116T1 (sl) | 2014-11-26 | 2017-10-30 | Clariant International Ltd | Oligonukleotidno zaporedje za uporabo v metabolnem inženiringu |
| JP6977231B2 (ja) | 2015-07-13 | 2021-12-08 | マラ リニューアブルズ コーポレーション | Ｃ５有機炭素の微生物による代謝の増強 |
| AU2016301365A1 (en) | 2015-08-05 | 2018-03-08 | Cargill, Incorporated | Xylose isomerase-modified yeast strains and methods for bioproduct production |
| CN109415716A (zh) | 2016-04-08 | 2019-03-01 | 纳幕尔杜邦公司 | 用于酵母的阿拉伯糖异构酶 |
| WO2018053058A1 (en) | 2016-09-14 | 2018-03-22 | Danisco Us Inc. | Lignocellulosic biomass fermentation-based processes |
| WO2018106656A1 (en) | 2016-12-06 | 2018-06-14 | Danisco Us Inc | Truncated lpmo enzymes and use thereof |
| EP3559210A1 (en) * | 2016-12-21 | 2019-10-30 | Vib Vzw | Xylose isomerases that confer efficient xylose fermentation capability to yeast |
| RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6716580B2 (en) | 1990-06-11 | 2004-04-06 | Somalogic, Inc. | Method for the automated generation of nucleic acid ligands |
| US5686276A (en) | 1995-05-12 | 1997-11-11 | E. I. Du Pont De Nemours And Company | Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism |
| JP4327909B2 (ja) | 1996-11-13 | 2009-09-09 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 組み換え生物による１，３―プロパンジオールの生産方法 |
| BR9815361B1 (pt) | 1997-12-02 | 2010-10-05 | método para produção de glicerol a partir de um microorganismo recombinante e microorganismo hospedeiro transformado. | |
| CA2380616C (en) | 1999-08-18 | 2011-05-24 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high titer |
| PT1468093E (pt) | 2002-01-23 | 2009-04-15 | Royal Nedalco B V | Fermentação de açúcares pentose |
| PL2345662T3 (pl) | 2003-05-02 | 2016-11-30 | Genetycznie modyfikowane gatunki drożdży i sposoby fermentacji z użyciem genetycznie modyfikowanych drożdży | |
| SE0302421D0 (sv) | 2003-09-11 | 2003-09-11 | Forskarpatent I Syd Ab | Construction of new xylose utilizing Saccharomyces cerevisiae strain |
| SE0400815D0 (sv) | 2004-03-26 | 2004-03-26 | Forskarpatent I Syd Ab | Traits in recombinant xylose-growing saccharomyces cerevisiae strains using genome-wide transcription analysis |
| SE0401303D0 (sv) | 2004-05-19 | 2004-05-19 | Forskarpatent I Syd Ab | Ethanol productivities of microbial strains in fermentation of dilute-acid hydrolyzates depend on their furan reduction capacities |
| WO2006096130A1 (en) | 2005-03-11 | 2006-09-14 | Forskarpatent I Syd Ab | Arabinose- and xylose-fermenting saccharomyces cerevisiae strains |
| EP1874942A4 (en) | 2005-04-27 | 2011-12-14 | Scandinavian Technology Group Ab | FERMENTATION OF GLUCOSE AND XYLOSE IN CELLULOSIC BIOMASS USING GENETICALLY MODIFIED SACCHAROMYCES CEREVISIAE AND SIMULTANEOUS TREATMENT OF SACCHARIFICATION AND CO-FERMENTATION |
| US9297028B2 (en) | 2005-09-29 | 2016-03-29 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| NZ566406A (en) | 2005-10-26 | 2012-04-27 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8828704B2 (en) | 2006-05-02 | 2014-09-09 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8206970B2 (en) | 2006-05-02 | 2012-06-26 | Butamax(Tm) Advanced Biofuels Llc | Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase |
| US8440449B2 (en) | 2008-09-30 | 2013-05-14 | The United States Of America, As Represented By The Secretary Of Agriculture | Transformed Saccharomyces cerevisiae engineered for xylose utilization |
| CA2767259A1 (en) | 2009-07-09 | 2011-01-13 | Verdezyne, Inc. | Engineered microorganisms with enhanced fermentation activity |
| US8772012B2 (en) | 2009-12-22 | 2014-07-08 | Kabushiki Kaisha Toyota Chuo Kenkyusho | Xylose isomerase and use thereof |
| CA2779163A1 (en) | 2009-12-30 | 2011-07-07 | Iogen Energy Corporation | Modified yeast strains exhibiting enhanced fermentation of lignocellulosic hydrolysates |
| WO2011149353A1 (en) | 2010-05-27 | 2011-12-01 | C5 Yeast Company B.V. | Yeast strains engineered to produce ethanol from acetic acid and glycerol |
| CN103124783A (zh) | 2010-06-03 | 2013-05-29 | 马斯科马公司 | 表达用于使用淀粉和纤维素进行联合生物加工的糖分解酶的酵母 |
| US8623623B2 (en) | 2010-06-29 | 2014-01-07 | E I Du Pont De Nemours And Company | Xylose utilization in recombinant Zymomonas |
| WO2012009272A2 (en) | 2010-07-14 | 2012-01-19 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| CN102174549B (zh) * | 2011-02-22 | 2012-10-10 | 山东大学 | 一种编码木糖异构酶的核酸分子及其编码的木糖异构酶 |
| WO2013003219A1 (en) | 2011-06-30 | 2013-01-03 | Codexis, Inc. | Pentose fermentation by a recombinant microorganism |
| BR112015001344A2 (pt) * | 2012-07-24 | 2017-07-04 | Bp Corp North America Inc | xilose isomerases e seus usos |
-
2013
- 2013-03-11 US US13/792,308 patent/US8669076B1/en not_active Expired - Fee Related
-
2014
- 2014-01-16 US US14/156,566 patent/US8906656B2/en not_active Expired - Fee Related
- 2014-03-10 JP JP2016500934A patent/JP6422478B2/ja not_active Expired - Fee Related
- 2014-03-10 CA CA2901021A patent/CA2901021A1/en not_active Abandoned
- 2014-03-10 MY MYPI2015702449A patent/MY168287A/en unknown
- 2014-03-10 BR BR112015020155A patent/BR112015020155A2/pt not_active Application Discontinuation
- 2014-03-10 AU AU2014249370A patent/AU2014249370A1/en not_active Abandoned
- 2014-03-10 CN CN201480012766.XA patent/CN105026559A/zh active Pending
- 2014-03-10 WO PCT/US2014/022267 patent/WO2014164392A1/en not_active Ceased
- 2014-03-10 EP EP14717258.9A patent/EP2970936B1/en not_active Not-in-force
-
2020
- 2020-02-13 AU AU2020201028A patent/AU2020201028A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| JP2016513457A (ja) | 2016-05-16 |
| WO2014164392A1 (en) | 2014-10-09 |
| CN105026559A (zh) | 2015-11-04 |
| BR112015020155A2 (pt) | 2017-08-29 |
| AU2020201028A1 (en) | 2020-03-05 |
| EP2970936A1 (en) | 2016-01-20 |
| AU2014249370A1 (en) | 2015-08-13 |
| EP2970936B1 (en) | 2017-12-13 |
| US8669076B1 (en) | 2014-03-11 |
| US20140256049A1 (en) | 2014-09-11 |
| US8906656B2 (en) | 2014-12-09 |
| MY168287A (en) | 2018-10-22 |
| JP6422478B2 (ja) | 2018-11-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8906656B2 (en) | Cow rumen xylose isomerases active in yeast cells | |
| US9371535B2 (en) | Bacterial xylose isomerases active in yeast cells | |
| Sibirny | Biotechnology of yeasts and filamentous fungi | |
| US12012604B2 (en) | Acetate consuming yeast cell | |
| WO2018172328A1 (en) | Improved glycerol free ethanol production | |
| WO2004067760A2 (en) | Xylose-fermenting recombinant yeast strains | |
| CN118325748A (zh) | 重组酵母细胞 | |
| US20140178954A1 (en) | Expression of xylose isomerase activity in yeast | |
| Vasylyshyn et al. | Construction of the Improved Producers of First-and Second-Generation Ethanol in Conventional and Non-conventional Yeasts | |
| US20190112590A1 (en) | Arabinose isomerases for yeast | |
| Vasylyshyn et al. | Construction of the Improved Producers | |
| US20250320529A1 (en) | Recombinant yeast cell | |
| EP4370688A1 (en) | Recombinant yeast cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20180312 |