CA2884687C - Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar - Google Patents
Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar Download PDFInfo
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- CA2884687C CA2884687C CA2884687A CA2884687A CA2884687C CA 2884687 C CA2884687 C CA 2884687C CA 2884687 A CA2884687 A CA 2884687A CA 2884687 A CA2884687 A CA 2884687A CA 2884687 C CA2884687 C CA 2884687C
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne des séquences dADN purifié et isolé qui présentent une activité daccroissement de la production de protéines, et plus particulièrement lutilisation de régions dattachement à la matrice (MAR) pour accroître lactivité de production de protéines dans une cellule eucaryote. Linvention concerne également un procédé pour lidentification de ces régions actives, en particulier de séquences nucléotidiques MAR, ainsi que lutilisation de ces séquences MAR actives caractérisées dans un nouveau procédé de transfection multiple.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51357403P | 2003-10-24 | 2003-10-24 | |
| US60/513,574 | 2003-10-24 | ||
| EP04002722 | 2004-02-06 | ||
| EP04002722.9 | 2004-02-06 | ||
| CA2826733A CA2826733C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2826733A Division CA2826733C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2884687A1 CA2884687A1 (fr) | 2005-05-06 |
| CA2884687C true CA2884687C (fr) | 2016-08-23 |
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Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2826733A Expired - Lifetime CA2826733C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884685A Expired - Fee Related CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884687A Expired - Lifetime CA2884687C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2826733A Expired - Lifetime CA2826733C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884685A Expired - Fee Related CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Country Status (7)
| Country | Link |
|---|---|
| KR (1) | KR20120069776A (fr) |
| CA (3) | CA2826733C (fr) |
| DK (4) | DK2298898T3 (fr) |
| ES (4) | ES2399878T3 (fr) |
| PT (4) | PT2292754E (fr) |
| SG (3) | SG10201500656RA (fr) |
| SI (4) | SI2292754T1 (fr) |
-
2004
- 2004-10-22 ES ES10178871T patent/ES2399878T3/es not_active Expired - Lifetime
- 2004-10-22 DK DK10178873.5T patent/DK2298898T3/da active
- 2004-10-22 PT PT10178871T patent/PT2292754E/pt unknown
- 2004-10-22 SI SI200432010T patent/SI2292754T1/sl unknown
- 2004-10-22 CA CA2826733A patent/CA2826733C/fr not_active Expired - Lifetime
- 2004-10-22 ES ES10178866T patent/ES2399877T3/es not_active Expired - Lifetime
- 2004-10-22 PT PT10178873T patent/PT2298898E/pt unknown
- 2004-10-22 DK DK10178871.9T patent/DK2292754T3/da active
- 2004-10-22 PT PT10178866T patent/PT2287302E/pt unknown
- 2004-10-22 SI SI200432012T patent/SI2298898T1/sl unknown
- 2004-10-22 SI SI200431878T patent/SI1959011T1/sl unknown
- 2004-10-22 ES ES08153753T patent/ES2383006T3/es not_active Expired - Lifetime
- 2004-10-22 KR KR1020127012896A patent/KR20120069776A/ko not_active Application Discontinuation
- 2004-10-22 ES ES10178873T patent/ES2400296T3/es not_active Expired - Lifetime
- 2004-10-22 SG SG10201500656RA patent/SG10201500656RA/en unknown
- 2004-10-22 DK DK08153753.2T patent/DK1959011T3/da active
- 2004-10-22 CA CA2884685A patent/CA2884685C/fr not_active Expired - Fee Related
- 2004-10-22 PT PT08153753T patent/PT1959011E/pt unknown
- 2004-10-22 SG SG2013076948A patent/SG195562A1/en unknown
- 2004-10-22 SG SG10201500657PA patent/SG10201500657PA/en unknown
- 2004-10-22 DK DK10178866.9T patent/DK2287302T3/da active
- 2004-10-22 SI SI200432011T patent/SI2287302T1/sl unknown
- 2004-10-22 CA CA2884687A patent/CA2884687C/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| SG10201500656RA (en) | 2015-04-29 |
| KR20120069776A (ko) | 2012-06-28 |
| DK2298898T3 (da) | 2013-04-08 |
| PT1959011E (pt) | 2012-05-25 |
| ES2399878T3 (es) | 2013-04-04 |
| CA2826733A1 (fr) | 2005-05-06 |
| SI2298898T1 (sl) | 2013-04-30 |
| CA2826733C (fr) | 2016-07-12 |
| PT2298898E (pt) | 2013-03-11 |
| CA2884685A1 (fr) | 2005-05-06 |
| DK1959011T3 (da) | 2012-07-02 |
| SI2287302T1 (sl) | 2013-04-30 |
| SG195562A1 (en) | 2013-12-30 |
| DK2292754T3 (da) | 2013-04-08 |
| SG10201500657PA (en) | 2015-04-29 |
| SI1959011T1 (sl) | 2012-06-29 |
| CA2884687A1 (fr) | 2005-05-06 |
| ES2400296T3 (es) | 2013-04-08 |
| ES2399877T3 (es) | 2013-04-04 |
| SI2292754T1 (sl) | 2013-04-30 |
| DK2287302T3 (da) | 2013-04-08 |
| PT2292754E (pt) | 2013-03-11 |
| PT2287302E (pt) | 2013-03-11 |
| CA2884685C (fr) | 2016-12-20 |
| ES2383006T3 (es) | 2012-06-15 |
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| Publication | Publication Date | Title |
|---|---|---|
| US10669562B2 (en) | High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences | |
| WO2008023247A2 (fr) | Régions de fixation de matrice (mar) pour augmenter une transcription et utilisations de celles-ci | |
| CA2884687C (fr) | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
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