CA2826733C - Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar - Google Patents
Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar Download PDFInfo
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Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La présente invention concerne des séquences dADN purifié et isolé présentant une activité daccroissement de la production de protéines, et plus particulièrement lutilisation de régions dattachement à la matrice (MAR) pour accroître lactivité de production de protéines dans une cellule eucaryote. Un procédé pour lidentification de ces régions actives, en particulier de séquences nucléotidiques MAR, ainsi que lutilisation de ces séquences MAR actives caractérisées dans un nouveau procédé de transfection multiple, est également décrit.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2884687A CA2884687C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884685A CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51357403P | 2003-10-24 | 2003-10-24 | |
| US60/513,574 | 2003-10-24 | ||
| EP04002722.9 | 2004-02-06 | ||
| EP04002722 | 2004-02-06 | ||
| CA2535836A CA2535836C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2535836A Division CA2535836C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2884687A Division CA2884687C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884685A Division CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2826733A1 CA2826733A1 (fr) | 2005-05-06 |
| CA2826733C true CA2826733C (fr) | 2016-07-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2884685A Expired - Fee Related CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884687A Active CA2884687C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2826733A Active CA2826733C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2884685A Expired - Fee Related CA2884685C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
| CA2884687A Active CA2884687C (fr) | 2003-10-24 | 2004-10-22 | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
Country Status (7)
| Country | Link |
|---|---|
| KR (1) | KR20120069776A (fr) |
| CA (3) | CA2884685C (fr) |
| DK (4) | DK2298898T3 (fr) |
| ES (4) | ES2400296T3 (fr) |
| PT (4) | PT2298898E (fr) |
| SG (3) | SG195562A1 (fr) |
| SI (4) | SI2298898T1 (fr) |
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2004
- 2004-10-22 DK DK10178873.5T patent/DK2298898T3/da active
- 2004-10-22 ES ES10178873T patent/ES2400296T3/es active Active
- 2004-10-22 SG SG2013076948A patent/SG195562A1/en unknown
- 2004-10-22 SI SI200432012T patent/SI2298898T1/sl unknown
- 2004-10-22 CA CA2884685A patent/CA2884685C/fr not_active Expired - Fee Related
- 2004-10-22 SG SG10201500657PA patent/SG10201500657PA/en unknown
- 2004-10-22 PT PT10178873T patent/PT2298898E/pt unknown
- 2004-10-22 SI SI200432011T patent/SI2287302T1/sl unknown
- 2004-10-22 PT PT08153753T patent/PT1959011E/pt unknown
- 2004-10-22 SI SI200431878T patent/SI1959011T1/sl unknown
- 2004-10-22 PT PT10178866T patent/PT2287302E/pt unknown
- 2004-10-22 ES ES10178866T patent/ES2399877T3/es active Active
- 2004-10-22 CA CA2884687A patent/CA2884687C/fr active Active
- 2004-10-22 DK DK08153753.2T patent/DK1959011T3/da active
- 2004-10-22 ES ES08153753T patent/ES2383006T3/es active Active
- 2004-10-22 KR KR1020127012896A patent/KR20120069776A/ko not_active Application Discontinuation
- 2004-10-22 ES ES10178871T patent/ES2399878T3/es active Active
- 2004-10-22 PT PT10178871T patent/PT2292754E/pt unknown
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- 2004-10-22 DK DK10178871.9T patent/DK2292754T3/da active
- 2004-10-22 CA CA2826733A patent/CA2826733C/fr active Active
- 2004-10-22 SI SI200432010T patent/SI2292754T1/sl unknown
- 2004-10-22 DK DK10178866.9T patent/DK2287302T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| DK2298898T3 (da) | 2013-04-08 |
| KR20120069776A (ko) | 2012-06-28 |
| ES2400296T3 (es) | 2013-04-08 |
| CA2884685C (fr) | 2016-12-20 |
| SI1959011T1 (sl) | 2012-06-29 |
| PT2292754E (pt) | 2013-03-11 |
| CA2884687A1 (fr) | 2005-05-06 |
| PT2287302E (pt) | 2013-03-11 |
| ES2399877T3 (es) | 2013-04-04 |
| ES2383006T3 (es) | 2012-06-15 |
| PT2298898E (pt) | 2013-03-11 |
| PT1959011E (pt) | 2012-05-25 |
| DK1959011T3 (da) | 2012-07-02 |
| SI2298898T1 (sl) | 2013-04-30 |
| SG10201500656RA (en) | 2015-04-29 |
| SG195562A1 (en) | 2013-12-30 |
| CA2884685A1 (fr) | 2005-05-06 |
| DK2287302T3 (da) | 2013-04-08 |
| SI2292754T1 (sl) | 2013-04-30 |
| SI2287302T1 (sl) | 2013-04-30 |
| DK2292754T3 (da) | 2013-04-08 |
| SG10201500657PA (en) | 2015-04-29 |
| CA2826733A1 (fr) | 2005-05-06 |
| CA2884687C (fr) | 2016-08-23 |
| ES2399878T3 (es) | 2013-04-04 |
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| EP2061883A2 (fr) | Régions de fixation de matrice (mar) pour augmenter une transcription et utilisations de celles-ci | |
| CA2826733C (fr) | Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar |
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