CA2884685C - Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar - Google Patents

Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar Download PDF

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Publication number
CA2884685C
CA2884685C CA2884685A CA2884685A CA2884685C CA 2884685 C CA2884685 C CA 2884685C CA 2884685 A CA2884685 A CA 2884685A CA 2884685 A CA2884685 A CA 2884685A CA 2884685 C CA2884685 C CA 2884685C
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CA
Canada
Prior art keywords
mar
sequence
purified
sequences
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2884685A
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English (en)
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CA2884685A1 (fr
Inventor
Nicolas Mermod
Pierre Alain Girod
Philipp Bucher
Duc-Quang Nguyen
David Calabrese
Damien Saugy
Stefania Puttini
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Selexis SA
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Selexis SA
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Publication date
Application filed by Selexis SA filed Critical Selexis SA
Publication of CA2884685A1 publication Critical patent/CA2884685A1/fr
Application granted granted Critical
Publication of CA2884685C publication Critical patent/CA2884685C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La présente invention porte sur des séquences d'ADN purifiées et isolées présentant une activité accrue de production de protéines et plus spécifiquement sur lutilisation des régions de fixation de matrice (MAR) servant à augmenter l'activité de production de protéines dans une cellule eucaryote. Une méthode est également révélée en vue de lidentification desdites régions actives, en particulier les séquences de nucléotides MAR, et lutilisation de ces séquences MAR actives caractérisées dans une nouvelle méthode de transfection multiple.
CA2884685A 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar Expired - Fee Related CA2884685C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US51357403P 2003-10-24 2003-10-24
US60/513,574 2003-10-24
EP04002722 2004-02-06
EP04002722.9 2004-02-06
CA2826733A CA2826733C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2826733A Division CA2826733C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Publications (2)

Publication Number Publication Date
CA2884685A1 CA2884685A1 (fr) 2005-05-06
CA2884685C true CA2884685C (fr) 2016-12-20

Family

ID=46149058

Family Applications (3)

Application Number Title Priority Date Filing Date
CA2826733A Expired - Lifetime CA2826733C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar
CA2884685A Expired - Fee Related CA2884685C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar
CA2884687A Expired - Lifetime CA2884687C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2826733A Expired - Lifetime CA2826733C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2884687A Expired - Lifetime CA2884687C (fr) 2003-10-24 2004-10-22 Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Country Status (7)

Country Link
KR (1) KR20120069776A (fr)
CA (3) CA2826733C (fr)
DK (4) DK2298898T3 (fr)
ES (4) ES2399878T3 (fr)
PT (4) PT2292754E (fr)
SG (3) SG10201500656RA (fr)
SI (4) SI2292754T1 (fr)

Also Published As

Publication number Publication date
SG10201500656RA (en) 2015-04-29
KR20120069776A (ko) 2012-06-28
DK2298898T3 (da) 2013-04-08
PT1959011E (pt) 2012-05-25
ES2399878T3 (es) 2013-04-04
CA2884687C (fr) 2016-08-23
CA2826733A1 (fr) 2005-05-06
SI2298898T1 (sl) 2013-04-30
CA2826733C (fr) 2016-07-12
PT2298898E (pt) 2013-03-11
CA2884685A1 (fr) 2005-05-06
DK1959011T3 (da) 2012-07-02
SI2287302T1 (sl) 2013-04-30
SG195562A1 (en) 2013-12-30
DK2292754T3 (da) 2013-04-08
SG10201500657PA (en) 2015-04-29
SI1959011T1 (sl) 2012-06-29
CA2884687A1 (fr) 2005-05-06
ES2400296T3 (es) 2013-04-08
ES2399877T3 (es) 2013-04-04
SI2292754T1 (sl) 2013-04-30
DK2287302T3 (da) 2013-04-08
PT2292754E (pt) 2013-03-11
PT2287302E (pt) 2013-03-11
ES2383006T3 (es) 2012-06-15

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US10669562B2 (en) High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of MAR sequences
EP2061883A2 (fr) Régions de fixation de matrice (mar) pour augmenter une transcription et utilisations de celles-ci
CA2884685C (fr) Transfert et expression genique a efficacite elevee dans des cellules de mammiferes au moyen d'une procedure de transfection multiple de sequences mar

Legal Events

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EEER Examination request

Effective date: 20150724

MKLA Lapsed

Effective date: 20221024