CA2870038C - Methods and compositions for extraction and storage of nucleic acids - Google Patents
Methods and compositions for extraction and storage of nucleic acids Download PDFInfo
- Publication number
- CA2870038C CA2870038C CA2870038A CA2870038A CA2870038C CA 2870038 C CA2870038 C CA 2870038C CA 2870038 A CA2870038 A CA 2870038A CA 2870038 A CA2870038 A CA 2870038A CA 2870038 C CA2870038 C CA 2870038C
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- CA
- Canada
- Prior art keywords
- solid matrix
- nucleic acids
- rna
- sample
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 52
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 52
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 35
- 238000003860 storage Methods 0.000 title claims abstract description 21
- 238000000605 extraction Methods 0.000 title claims abstract description 12
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- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 claims description 7
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
- B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
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| US13/460,076 | 2012-04-30 | ||
| US13/460,076 US9044738B2 (en) | 2012-04-30 | 2012-04-30 | Methods and compositions for extraction and storage of nucleic acids |
| PCT/US2013/038576 WO2013165870A1 (en) | 2012-04-30 | 2013-04-29 | Methods and compositions for extraction and storage of nucleic acids |
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| CA2870038A1 CA2870038A1 (en) | 2013-11-07 |
| CA2870038C true CA2870038C (en) | 2021-08-03 |
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| EP (1) | EP2844755B1 (OSRAM) |
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| CN (1) | CN104254776B (OSRAM) |
| CA (1) | CA2870038C (OSRAM) |
| ES (1) | ES2609763T3 (OSRAM) |
| IN (1) | IN2014DN08223A (OSRAM) |
| WO (1) | WO2013165870A1 (OSRAM) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9480966B2 (en) | 2012-04-30 | 2016-11-01 | General Electric Company | Substrates and methods for collection, stabilization and elution of biomolecules |
| US10829291B2 (en) * | 2012-06-20 | 2020-11-10 | Thermo Fischer Scientific Baltics UAB | Method to prevent silica-based column aging |
| AU2014307875B2 (en) * | 2013-08-16 | 2020-07-02 | Global Life Sciences Solutions Operations UK Ltd | Methods and compositions for extraction and storage of nucleic acids |
| EP3942931A1 (en) | 2014-06-10 | 2022-01-26 | Biomatrica, INC. | Stabilization of thrombocytes at ambient temperatures |
| JP2018502281A (ja) * | 2014-11-20 | 2018-01-25 | ジーイー・ヘルスケア・ユーケイ・リミテッド | 固体支持体材料に固定された認知症及びアルツハイマー病関連バイオマーカーの検出 |
| GB201505516D0 (en) * | 2015-03-31 | 2015-05-13 | Ge Healthcare Uk Ltd | Improvements in and relating to biological sample collectors and handling thereof |
| FI4035762T3 (fi) | 2015-09-09 | 2023-12-04 | Drawbridge Health Inc | Laitteita näytteiden keräämistä, stabilointia ja säilytystä varten |
| US10000742B2 (en) * | 2015-11-19 | 2018-06-19 | General Electric Company | Device and method of collection for RNA viruses |
| EP4242628B1 (en) | 2015-12-08 | 2025-07-02 | Biomatrica, Inc. | Reduction of erythrocyte sedimentation rate |
| CN109154544B (zh) * | 2016-03-18 | 2024-05-14 | 蜂鸟诊断有限责任公司 | 使用亲水性聚合材料的rna级分的纯化 |
| CN105961375A (zh) * | 2016-07-13 | 2016-09-28 | 北京中科唯新生物医学研究所有限公司 | 一种唾液保存液及其制备方法和用途 |
| US11166658B2 (en) * | 2016-07-28 | 2021-11-09 | Invitae Corporation | Blood sampling system and method |
| WO2018081161A1 (en) * | 2016-10-24 | 2018-05-03 | Biomatrica, Inc. | Stabilization of nucleic acids on paper |
| GB2590814B (en) | 2017-01-10 | 2021-11-03 | Drawbridge Health Inc | Devices, systems, and methods for sample collection |
| CN109182332A (zh) * | 2018-09-30 | 2019-01-11 | 浙江天杭生物科技股份有限公司 | 一种从临床样品中快速简便提取核酸的方法 |
| SG11202104814XA (en) * | 2019-01-25 | 2021-08-30 | Sekisui Chemical Co Ltd | Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip |
| EP3969585A1 (en) * | 2020-05-14 | 2022-03-23 | Bioecho Life Sciences GmbH | Isolation of nucleic acids at elevated temperatures |
| US20230332132A1 (en) | 2020-08-28 | 2023-10-19 | Kao Corporation | Rna storage method |
| GB202211645D0 (en) | 2022-08-09 | 2022-09-21 | Hemodx As | Kit |
| SE2351278A1 (en) * | 2023-11-08 | 2025-05-09 | Sequrna Ab | Dry storage |
Family Cites Families (59)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4540506A (en) | 1983-04-15 | 1985-09-10 | Genex Corporation | Composition for cleaning drains clogged with deposits containing hair |
| CA1255197A (en) * | 1984-09-24 | 1989-06-06 | Joseph L. Orlik | Leukocyte differentiation composition and method |
| US5173422A (en) | 1986-08-22 | 1992-12-22 | Miles Inc. | Monoclonal antibodies specific for human glycoalbumin |
| US6627226B2 (en) | 1988-10-05 | 2003-09-30 | Whatman, Inc. | Dry solid medium for storage and analysis of genetic material |
| US5756126A (en) | 1991-05-29 | 1998-05-26 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| US5807527A (en) | 1991-05-29 | 1998-09-15 | Flinders Technologies Pty. Ltd. | Solid medium and method for DNA storage |
| WO1996039813A1 (en) | 1995-06-07 | 1996-12-19 | Flinders Technologies Pty. Ltd. | Dry solid medium for storage and analysis of genetic material |
| JPH11510393A (ja) | 1995-08-09 | 1999-09-14 | インスティチュート ナショナル ドゥ ラ セント エト ドゥ ラ リサーチェ メディカル | 白血病マーカーとしておよび乳癌の予後において有用な単離された核酸分子 |
| US5939259A (en) | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| US6204375B1 (en) | 1998-07-31 | 2001-03-20 | Ambion, Inc. | Methods and reagents for preserving RNA in cell and tissue samples |
| DE19836559A1 (de) | 1998-08-12 | 2000-03-23 | Antigen Gmbh | Gefäß zur Entnahme von Blut |
| US20010039010A1 (en) | 1998-09-03 | 2001-11-08 | Leigh Alexander Burgoyne | Sample collection medium incorporating material for sample visualization |
| WO2000066605A2 (en) | 1999-04-30 | 2000-11-09 | Cyclops Genome Sciences Limited | Polynucleotides |
| AU4353500A (en) | 1999-04-30 | 2000-11-17 | Whatman, Inc. | Substrate including anionic detergent for purifying nucleic acid |
| EP1220944B1 (en) | 1999-09-24 | 2010-09-15 | Ambion, Inc. | Nuclease inhibitor cocktail |
| DE10006662A1 (de) | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| US6602718B1 (en) | 2000-11-08 | 2003-08-05 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
| US7531362B2 (en) | 2001-06-07 | 2009-05-12 | Medmira Inc. | Rapid diagnostic assay |
| WO2003020924A2 (en) | 2001-09-05 | 2003-03-13 | Whatman Plc | Stable storage of proteins |
| GB0129816D0 (en) * | 2001-12-13 | 2002-01-30 | The Technology Partnership Plc | Testing device for chemical or biochemical analysis |
| US7482116B2 (en) | 2002-06-07 | 2009-01-27 | Dna Genotek Inc. | Compositions and methods for obtaining nucleic acids from sputum |
| EP1552005A4 (en) | 2002-07-10 | 2010-12-15 | Massachusetts Inst Technology | DEVICE AND METHOD FOR ISOLATING A NUCLEIC ACID FROM A SAMPLE |
| DE10231659B4 (de) | 2002-07-12 | 2006-01-19 | Preanalytix Gmbh | Zusammensetzung zum Binden von Nukleinsäure an eine Festphase |
| GB0217963D0 (en) | 2002-08-02 | 2002-09-11 | Cyclops Genome Sciences Ltd | Purification of nucleic acids |
| US7267950B2 (en) * | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
| JP2007502218A (ja) * | 2003-05-23 | 2007-02-08 | ユィロス・パテント・アクチボラグ | 親水性／疎水性表面 |
| US7250270B2 (en) | 2003-06-13 | 2007-07-31 | Ambion, Inc. | Methods and compositions for preparing tissue samples for RNA extraction |
| ATE514791T1 (de) | 2003-07-09 | 2011-07-15 | Genvault Corp | Raumtemperatur-eluierung der nukleinsäuren |
| JP3714940B2 (ja) | 2003-11-07 | 2005-11-09 | 株式会社日立ハイテクノロジーズ | Ｒｎａ抽出方法、および生体材料の分析方法 |
| US20080176209A1 (en) | 2004-04-08 | 2008-07-24 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| US20060099567A1 (en) | 2004-04-08 | 2006-05-11 | Biomatrica, Inc. | Integration of sample storage and sample management for life science |
| BRPI0509693A (pt) | 2004-04-09 | 2007-10-09 | Res Think Tank Inc | dispositivo e método para coletar, armazenar ou transportar um espécime biológico, método para recuperar um espécime biológico, e, kit para coletar, armazenar e transportar um espécime biológico |
| DE102004021780B4 (de) | 2004-04-30 | 2008-10-02 | Siemens Ag | Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien |
| WO2005116081A2 (en) * | 2004-05-24 | 2005-12-08 | Genvault Corporation | Stable protein storage and stable nucleic acid storage in recoverable form |
| US7776530B2 (en) | 2004-06-29 | 2010-08-17 | Wallac Oy | Integrated nucleic acid analysis |
| US20090208919A1 (en) | 2005-01-21 | 2009-08-20 | Argylla Technologies, Llp | Particle matrix for storage of biomolecules |
| US8158357B2 (en) | 2005-03-16 | 2012-04-17 | Dna Genotek Inc. | Compositions and method for storage of nucleic acid from bodily fluids |
| GB0505909D0 (en) | 2005-03-23 | 2005-04-27 | Univ Leeds | Formulations |
| US20070015165A1 (en) | 2005-07-13 | 2007-01-18 | Sigma-Aldrich Co. | Method for the isolation of RNA from biological sources |
| JP4699868B2 (ja) * | 2005-11-04 | 2011-06-15 | 株式会社日立ハイテクノロジーズ | 核酸精製方法及び核酸精製器具 |
| EP1963526A4 (en) | 2005-12-09 | 2009-11-18 | Promega Corp | PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX |
| US8080645B2 (en) * | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| EP2384432B1 (en) * | 2007-06-21 | 2016-12-28 | Gen-Probe Incorporated | Instrument and receptacles for performing processes |
| US20090053704A1 (en) | 2007-08-24 | 2009-02-26 | Natalia Novoradovskaya | Stabilization of nucleic acids on solid supports |
| WO2009070558A1 (en) * | 2007-11-30 | 2009-06-04 | Ge Healthcare Bio-Sciences Corp. | Method for isolation of genomic dna, rna and proteins from a single sample |
| WO2009117167A1 (en) | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| US20100209957A1 (en) | 2008-06-20 | 2010-08-19 | Genvault Corporation | Biosample storage devices and methods of use thereof |
| EP2334792B1 (en) | 2008-09-12 | 2020-09-09 | GenTegra LLC | Matrices and media for storage and stabilization of biomolecules |
| EP2412020B1 (en) * | 2009-03-24 | 2020-09-30 | University Of Chicago | Slip chip device and methods |
| EP2430195B1 (en) * | 2009-05-11 | 2019-01-23 | Biomatrica, INC. | Compositions and methods for biological sample storage |
| AU2011244348B2 (en) | 2010-04-20 | 2015-02-12 | Octapharma Ag | New stabilizing agent for pharmaceutical proteins |
| EP2388312A1 (en) | 2010-05-17 | 2011-11-23 | Curetis AG | Universally applicable lysis buffer and processing methods for the lysis of bodily samples |
| CA2806670A1 (en) | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
| WO2012075471A1 (en) | 2010-12-04 | 2012-06-07 | Hui-Yu Liu | Rna stability enhancer |
| US20120152743A1 (en) | 2010-12-17 | 2012-06-21 | General Electric Company | Method for electroeluting genetic material from dried samples |
| GB201103257D0 (en) | 2011-02-25 | 2011-04-13 | Ge Healthcare Uk Ltd | Paper support and method of recovering biological material therefrom |
| GB2496122A (en) | 2011-10-31 | 2013-05-08 | Ge Healthcare Bio Sciences Ab | Biological sample preservation on paper |
| CN103173432B (zh) * | 2011-12-22 | 2020-08-04 | 通用电气公司 | 分离核酸的方法及装置 |
| US9040675B2 (en) | 2012-04-30 | 2015-05-26 | General Electric Company | Formulations for nucleic acid stabilization on solid substrates |
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| CN104254776A (zh) | 2014-12-31 |
| EP2844755A4 (en) | 2015-03-11 |
| US9044738B2 (en) | 2015-06-02 |
| EP2844755B1 (en) | 2016-11-16 |
| IN2014DN08223A (OSRAM) | 2015-05-15 |
| CA2870038A1 (en) | 2013-11-07 |
| JP2020092709A (ja) | 2020-06-18 |
| WO2013165870A1 (en) | 2013-11-07 |
| US20130289265A1 (en) | 2013-10-31 |
| CN104254776B (zh) | 2016-09-28 |
| JP6248095B2 (ja) | 2017-12-13 |
| EP2844755A1 (en) | 2015-03-11 |
| JP2015518569A (ja) | 2015-07-02 |
| JP6847365B2 (ja) | 2021-03-24 |
| ES2609763T3 (es) | 2017-04-24 |
| JP2018068303A (ja) | 2018-05-10 |
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