CA2867489A1 - Methods and composition for sequencing modified nucleic acids - Google Patents

Methods and composition for sequencing modified nucleic acids Download PDF

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Publication number
CA2867489A1
CA2867489A1 CA2867489A CA2867489A CA2867489A1 CA 2867489 A1 CA2867489 A1 CA 2867489A1 CA 2867489 A CA2867489 A CA 2867489A CA 2867489 A CA2867489 A CA 2867489A CA 2867489 A1 CA2867489 A1 CA 2867489A1
Authority
CA
Canada
Prior art keywords
sequence
data
nucleic acid
sequencing
nucleic acids
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2867489A
Other languages
English (en)
French (fr)
Inventor
Jonas Korlach
Stephen Turner
Tyson A. Clark
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pacific Biosciences of California Inc
Original Assignee
Pacific Biosciences of California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pacific Biosciences of California Inc filed Critical Pacific Biosciences of California Inc
Publication of CA2867489A1 publication Critical patent/CA2867489A1/en
Abandoned legal-status Critical Current

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A90/00Technologies having an indirect contribution to adaptation to climate change
    • Y02A90/10Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
CA2867489A 2012-03-30 2013-03-18 Methods and composition for sequencing modified nucleic acids Abandoned CA2867489A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201261617999P 2012-03-30 2012-03-30
US61/617,999 2012-03-30
US201261721206P 2012-11-01 2012-11-01
US201261721339P 2012-11-01 2012-11-01
US201361789354P 2013-03-15 2013-03-15
US201361799237P 2013-03-15 2013-03-15
PCT/US2013/032816 WO2013148400A1 (en) 2012-03-30 2013-03-18 Methods and composition for sequencing modified nucleic acids

Publications (1)

Publication Number Publication Date
CA2867489A1 true CA2867489A1 (en) 2013-10-03

Family

ID=49261113

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2867489A Abandoned CA2867489A1 (en) 2012-03-30 2013-03-18 Methods and composition for sequencing modified nucleic acids

Country Status (4)

Country Link
EP (1) EP2831283A4 (de)
AU (1) AU2013240166A1 (de)
CA (1) CA2867489A1 (de)
WO (1) WO2013148400A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
JP6573899B2 (ja) * 2013-11-17 2019-09-11 クアンタム−エスアイ インコーポレイテッドQuantum−Si Incorporated 分子をプローブし、検出し、及び分析するための、外部光源を備えた集積デバイス
CA2964937A1 (en) * 2014-10-22 2016-04-28 Ibis Biosciences, Inc. Bacterial epigenomic analysis
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
WO2016100049A1 (en) 2014-12-18 2016-06-23 Edico Genome Corporation Chemically-sensitive field effect transistor
EP3459115A4 (de) 2016-05-16 2020-04-08 Agilome, Inc. Graphen-fet-vorrichtungen, systeme und verfahren zur verwendung davon zur sequenzierung von nukleinsäuren
BR112018074572B1 (pt) * 2016-06-01 2024-02-27 Quantum-Si Incorporated Métodos de identificação de nucleotídeos e para calibrar um instrumento de sequenciamento, meio de armazenamento legível em computador não transitório, dispositivo e instrumento de sequenciamento
EP3366780B1 (de) 2017-02-23 2020-05-06 Siemens Healthcare GmbH Sequenzanalyse von einzelmolekülen und hochempfindliche methylierungsanalyse zur gewebespezifischen analyse
JP7304852B2 (ja) * 2017-11-03 2023-07-07 ガーダント ヘルス, インコーポレイテッド 脱アミノ化に誘導される配列エラーの補正
US11538556B2 (en) 2018-01-26 2022-12-27 Quantum-Si Incorporated Machine learning enabled pulse and base calling for sequencing devices
WO2019226689A1 (en) 2018-05-22 2019-11-28 Axbio Inc. Methods, systems, and compositions for nucleic acid sequencing
US20230295608A1 (en) * 2022-03-17 2023-09-21 Regeneron Pharmaceuticals, Inc. Methods for Characterization of Viral Genome Using Base Modifications

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0711303B2 (de) 1993-07-30 2009-06-10 Affymax, Inc. Biotinylierung von proteinen
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
WO2005014506A2 (en) 2003-08-06 2005-02-17 Department Of Science & Technology (Dst) A process for the manufacture of a bio-release fertiliser of an anionic micro nutrient viz molibdenum
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US7399614B2 (en) 2004-06-30 2008-07-15 Applera Corporation 5-methylcytosine detection, compositions and methods therefor
JP2008513782A (ja) 2004-09-17 2008-05-01 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 分子解析のための装置及び方法
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
JP2009511862A (ja) 2005-09-30 2009-03-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 反応性表面、基質および同表面、基質の製造および使用方法
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
EP3936857B1 (de) 2006-09-01 2023-06-21 Pacific Biosciences Of California, Inc. Substrate, systeme und verfahren zur analyse von materialien
US20080277595A1 (en) 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
CA2689626C (en) * 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CN101802220B (zh) 2007-07-26 2013-07-31 加利福尼亚太平洋生物科学股份有限公司 分子冗余测序法
US8252911B2 (en) 2008-02-12 2012-08-28 Pacific Biosciences Of California, Inc. Compositions and methods for use in analytical reactions
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
AU2009223702B2 (en) 2008-03-13 2013-08-22 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
CA2720247C (en) * 2008-03-31 2020-07-14 Pacific Biosciences Of California, Inc. Single molecule loading methods and compositions
WO2009145820A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
CA3063032A1 (en) 2008-09-16 2010-03-25 Pacific Biosciences Of California, Inc. Substrates and optical systems and methods of use thereof
US8481264B2 (en) 2008-09-19 2013-07-09 Pacific Biosciences Of California, Inc. Immobilized nucleic acid complexes for sequence analysis
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
EP2370598B1 (de) * 2008-12-11 2017-02-15 Pacific Biosciences Of California, Inc. Klassifizierung von nukleinsäuretemplaten
JP5439822B2 (ja) 2009-01-15 2014-03-12 独立行政法人物質・材料研究機構 Ni基単結晶超合金
US9017937B1 (en) 2009-04-10 2015-04-28 Pacific Biosciences Of California, Inc. Nanopore sequencing using ratiometric impedance
AU2010245304B2 (en) 2009-04-27 2015-06-04 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
WO2011103421A1 (en) * 2010-02-18 2011-08-25 Shuber Anthony P Compositions and methods for treating cancer
US20130138358A1 (en) 2010-02-24 2013-05-30 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US9165109B2 (en) 2010-02-24 2015-10-20 Pacific Biosciences Of California, Inc. Sequence assembly and consensus sequence determination

Also Published As

Publication number Publication date
AU2013240166A1 (en) 2014-10-30
EP2831283A4 (de) 2015-11-04
WO2013148400A1 (en) 2013-10-03
EP2831283A1 (de) 2015-02-04

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Effective date: 20190319