CA2860739A1 - Enzyme cascade methods for e-trace assay signal amplification - Google Patents
Enzyme cascade methods for e-trace assay signal amplification Download PDFInfo
- Publication number
- CA2860739A1 CA2860739A1 CA2860739A CA2860739A CA2860739A1 CA 2860739 A1 CA2860739 A1 CA 2860739A1 CA 2860739 A CA2860739 A CA 2860739A CA 2860739 A CA2860739 A CA 2860739A CA 2860739 A1 CA2860739 A1 CA 2860739A1
- Authority
- CA
- Canada
- Prior art keywords
- enzyme
- peroxide
- eam
- target
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/27—Association of two or more measuring systems or cells, each measuring a different parameter, where the measurement results may be either used independently, the systems or cells being physically associated, or combined to produce a value for a further parameter
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3271—Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood
- G01N27/3273—Devices therefor, e.g. test element readers, circuitry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/4166—Systems measuring a particular property of an electrolyte
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
- G01N33/5438—Electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Electrochemistry (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261584563P | 2012-01-09 | 2012-01-09 | |
US61/584,563 | 2012-01-09 | ||
PCT/US2013/020836 WO2013106434A1 (en) | 2012-01-09 | 2013-01-09 | Enzyme cascade methods for e-trace assay signal amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2860739A1 true CA2860739A1 (en) | 2013-07-18 |
Family
ID=47559760
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2860739A Abandoned CA2860739A1 (en) | 2012-01-09 | 2013-01-09 | Enzyme cascade methods for e-trace assay signal amplification |
Country Status (5)
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8951400B2 (en) | 2007-10-17 | 2015-02-10 | Ohmx Corporation | Chemistry used in biosensors |
EP2462238B1 (en) | 2009-08-07 | 2013-07-17 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) immunoassay |
WO2012100078A1 (en) | 2011-01-19 | 2012-07-26 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) immmunoassay |
WO2013059293A1 (en) | 2011-10-17 | 2013-04-25 | Ohmx Corporation | Single, direct detection of hemoglobin a1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay |
EP2773650A1 (en) | 2011-11-04 | 2014-09-10 | Ohmx Corporation | Novel chemistry used in biosensors |
JP6199387B2 (ja) | 2012-07-27 | 2017-09-20 | オームクス コーポレイション | 構成要素の均一反応後の単分子層の電子測定 |
EP2877851A1 (en) | 2012-07-27 | 2015-06-03 | Ohmx Corporation | Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes |
Family Cites Families (82)
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US407828A (en) | 1889-07-30 | Tire-tightener | ||
US332565A (en) | 1885-12-15 | Self-binding grain-harvester | ||
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DE3061860D1 (en) | 1979-04-24 | 1983-03-17 | Marcel Jozefonvicz | Process for the determination of proteases and antiproteases |
US4469863A (en) | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
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US5235033A (en) | 1985-03-15 | 1993-08-10 | Anti-Gene Development Group | Alpha-morpholino ribonucleoside derivatives and polymers thereof |
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US5173373A (en) | 1989-09-14 | 1992-12-22 | Yamaha Hatsudoki Kabushiki Kaisha | Gasket for fuel cell |
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EP2462238B1 (en) | 2009-08-07 | 2013-07-17 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) immunoassay |
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WO2012100078A1 (en) | 2011-01-19 | 2012-07-26 | Ohmx Corporation | Enzyme triggered redox altering chemical elimination (e-trace) immmunoassay |
WO2013059293A1 (en) | 2011-10-17 | 2013-04-25 | Ohmx Corporation | Single, direct detection of hemoglobin a1c percentage using enzyme triggered redox altering chemical elimination (e-trace) immunoassay |
EP2773650A1 (en) | 2011-11-04 | 2014-09-10 | Ohmx Corporation | Novel chemistry used in biosensors |
EP2877851A1 (en) | 2012-07-27 | 2015-06-03 | Ohmx Corporation | Electric measurement of monolayers following pro-cleave detection of presence and activity of enzymes and other target analytes |
JP6199387B2 (ja) | 2012-07-27 | 2017-09-20 | オームクス コーポレイション | 構成要素の均一反応後の単分子層の電子測定 |
US9659398B2 (en) | 2013-03-15 | 2017-05-23 | Dreamworks Animation Llc | Multiple visual representations of lighting effects in a computer animation scene |
-
2013
- 2013-01-09 US US13/737,634 patent/US9250203B2/en not_active Expired - Fee Related
- 2013-01-09 CA CA2860739A patent/CA2860739A1/en not_active Abandoned
- 2013-01-09 JP JP2014551418A patent/JP6276706B2/ja not_active Expired - Fee Related
- 2013-01-09 EP EP13700357.0A patent/EP2802669A1/en not_active Withdrawn
- 2013-01-09 WO PCT/US2013/020836 patent/WO2013106434A1/en active Application Filing
-
2015
- 2015-12-18 US US14/974,736 patent/US20160202203A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
JP6276706B2 (ja) | 2018-02-07 |
WO2013106434A9 (en) | 2014-03-13 |
JP2015508500A (ja) | 2015-03-19 |
EP2802669A1 (en) | 2014-11-19 |
WO2013106434A1 (en) | 2013-07-18 |
US20130264220A1 (en) | 2013-10-10 |
US20160202203A1 (en) | 2016-07-14 |
US9250203B2 (en) | 2016-02-02 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20171117 |
|
FZDE | Discontinued |
Effective date: 20200109 |