CA2847151C - Assessing stem cells for contamination using microrna expression profiles - Google Patents

Assessing stem cells for contamination using microrna expression profiles Download PDF

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Publication number
CA2847151C
CA2847151C CA2847151A CA2847151A CA2847151C CA 2847151 C CA2847151 C CA 2847151C CA 2847151 A CA2847151 A CA 2847151A CA 2847151 A CA2847151 A CA 2847151A CA 2847151 C CA2847151 C CA 2847151C
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cells
micro
cell
stem cells
rna
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English (en)
French (fr)
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CA2847151A1 (en
Inventor
Vincent O'brien
Chris Hillier
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Sistemic Scotland Ltd
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Sistemic Scotland Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2847151A 2010-08-23 2011-08-19 Assessing stem cells for contamination using microrna expression profiles Active CA2847151C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB1014049.9A GB201014049D0 (en) 2010-08-23 2010-08-23 Cell characterisation
GB1014049.9 2010-08-23
PCT/GB2011/001241 WO2012025709A1 (en) 2010-08-23 2011-08-19 Cell characterisation

Publications (2)

Publication Number Publication Date
CA2847151A1 CA2847151A1 (en) 2012-03-01
CA2847151C true CA2847151C (en) 2023-11-28

Family

ID=42984495

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2847151A Active CA2847151C (en) 2010-08-23 2011-08-19 Assessing stem cells for contamination using microrna expression profiles

Country Status (12)

Country Link
US (4) US20130196875A1 (https=)
EP (1) EP2609217B1 (https=)
JP (1) JP2013535979A (https=)
KR (1) KR20140006775A (https=)
CN (1) CN103403180A (https=)
AU (1) AU2011294936A1 (https=)
BR (1) BR112013004202A2 (https=)
CA (1) CA2847151C (https=)
GB (1) GB201014049D0 (https=)
IL (1) IL224888B (https=)
SG (2) SG190837A1 (https=)
WO (1) WO2012025709A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2859119A2 (en) * 2012-06-12 2015-04-15 Institute for Systems Biology Complex rna composition of bodily fluids
US11293065B2 (en) 2016-03-14 2022-04-05 Aelan Cell Technologies, Inc. Compositions and methods for the quality control of stem cell preparations
CA3062963A1 (en) * 2016-05-09 2017-11-16 Sistemic Scotland Ltd Functional prediction of cellular functions by means of microrna expression profiling in mesenchymal stem cells
JP6629770B2 (ja) * 2017-01-19 2020-01-15 シスメックス株式会社 細胞の分化状態を評価する方法
JP6918062B2 (ja) * 2017-01-19 2021-08-11 シスメックス株式会社 細胞の分化状態を評価する方法
WO2019141878A1 (en) * 2018-01-22 2019-07-25 Sistemic Scotland Ltd Cell contamination assay
WO2020072545A1 (en) * 2018-10-01 2020-04-09 Lundquist Institute For Biomedical Innovation At Harbor-Ucla Medical Center MicroRNA TRANSCRIPTIONAL SIGNATURE (miR-TS) EVALUATION OF STEM CELLS
CN113658636B (zh) * 2021-07-22 2025-08-05 未来智人再生医学研究院(广州)有限公司 一种评估多能干细胞质量的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050123952A1 (en) * 2003-09-04 2005-06-09 Griffey Richard H. Methods of rapid detection and identification of bioagents using microRNA
CA2566519C (en) * 2004-05-14 2020-04-21 Rosetta Genomics Ltd. Micrornas and uses thereof
EP1966390A1 (en) * 2005-12-29 2008-09-10 Exiqon A/S Detection of tissue origin of cancer
CN101316935B (zh) * 2006-11-28 2012-02-29 博奥生物有限公司 一种用于诊断食道癌的芯片
WO2008073915A2 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Micrornas differentially expressed in leukemia and uses thereof
WO2008112283A2 (en) * 2007-03-12 2008-09-18 Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Microrna profiling of androgen responsiveness for predicting the appropriate prostate cancer treatment
US8361714B2 (en) * 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
WO2009062503A1 (en) * 2007-11-14 2009-05-22 Dandrit Biotech A/S Micro rnas as markers of the functional state of a dendritic cell
JP2011516033A (ja) * 2008-02-28 2011-05-26 ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイション 急性骨髄性性白血病（ａｍｌ）における細胞遺伝学および予後と関連するマイクロｒｎａシグネチャーおよびその使用
US20100003674A1 (en) * 2008-07-03 2010-01-07 Cope Frederick O Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions
EP2387617B1 (en) * 2009-01-19 2014-10-29 Sistemic Scotland Limited Methods employing non-coding rna expression assays

Also Published As

Publication number Publication date
IL224888B (en) 2020-10-29
EP2609217B1 (en) 2019-03-27
AU2011294936A1 (en) 2013-04-11
SG10201506595QA (en) 2015-10-29
CN103403180A (zh) 2013-11-20
JP2013535979A (ja) 2013-09-19
KR20140006775A (ko) 2014-01-16
GB201014049D0 (en) 2010-10-06
US20200080148A1 (en) 2020-03-12
BR112013004202A2 (pt) 2019-09-24
EP2609217A1 (en) 2013-07-03
US20230183803A1 (en) 2023-06-15
US20130196875A1 (en) 2013-08-01
SG190837A1 (en) 2013-07-31
WO2012025709A1 (en) 2012-03-01
CA2847151A1 (en) 2012-03-01
US20170218446A1 (en) 2017-08-03

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