CA2778329C - Device and apparatus for assaying nucleic acids - Google Patents

Device and apparatus for assaying nucleic acids Download PDF

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Publication number
CA2778329C
CA2778329C CA2778329A CA2778329A CA2778329C CA 2778329 C CA2778329 C CA 2778329C CA 2778329 A CA2778329 A CA 2778329A CA 2778329 A CA2778329 A CA 2778329A CA 2778329 C CA2778329 C CA 2778329C
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CA
Canada
Prior art keywords
well
channel
nucleic acid
diluent
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2778329A
Other languages
English (en)
French (fr)
Other versions
CA2778329A1 (en
Inventor
Philip Wakeley
Graham Gutsell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Environment Food and Rural Affairs
Original Assignee
UK Secretary of State for Environment Food and Rural Affairs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Environment Food and Rural Affairs filed Critical UK Secretary of State for Environment Food and Rural Affairs
Publication of CA2778329A1 publication Critical patent/CA2778329A1/en
Application granted granted Critical
Publication of CA2778329C publication Critical patent/CA2778329C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16Reagents, handling or storing thereof
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/041Connecting closures to device or container
    • B01L2300/042Caps; Plugs
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406Moving fluids with specific forces or mechanical means specific forces capillary forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5023Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2778329A 2009-11-02 2010-11-02 Device and apparatus for assaying nucleic acids Active CA2778329C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0919159.4 2009-11-02
GBGB0919159.4A GB0919159D0 (en) 2009-11-02 2009-11-02 Device and apparatus
PCT/GB2010/051832 WO2011051735A2 (en) 2009-11-02 2010-11-02 Device and apparatus

Publications (2)

Publication Number Publication Date
CA2778329A1 CA2778329A1 (en) 2011-05-05
CA2778329C true CA2778329C (en) 2018-09-04

Family

ID=41435004

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2778329A Active CA2778329C (en) 2009-11-02 2010-11-02 Device and apparatus for assaying nucleic acids

Country Status (10)

Country Link
US (1) US9163279B2 (enExample)
EP (1) EP2496351B1 (enExample)
JP (1) JP5934100B2 (enExample)
CN (1) CN102655938B (enExample)
AU (1) AU2010311138B2 (enExample)
CA (1) CA2778329C (enExample)
ES (1) ES2712666T3 (enExample)
GB (1) GB0919159D0 (enExample)
TR (1) TR201900390T4 (enExample)
WO (1) WO2011051735A2 (enExample)

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US20140170678A1 (en) * 2012-12-17 2014-06-19 Leukodx Ltd. Kits, compositions and methods for detecting a biological condition
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US10946376B2 (en) 2013-07-05 2021-03-16 Thinxxs Microtechnology Ag Carrier element for introducing a dry substance into a flow cell
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HK1222597A1 (zh) * 2013-10-22 2017-07-07 艾博生物医药(杭州)有限公司 用於检测液体样本中的分析物的检测装置
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FR3032719B1 (fr) * 2015-02-13 2020-05-15 Centre National De La Recherche Scientifique Dispositif de diagnostic par amplification isotherme d'adn
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GB201616718D0 (en) * 2016-09-30 2016-11-16 Microbiosensor Limited Microbial sensing devices
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FR3075823A1 (fr) * 2017-12-21 2019-06-28 Nanobiose Procede d’analyse d’un echantillon cellulaire au moyen d’un laboratoire sur puce-micro-fluidique
FR3075817B1 (fr) * 2017-12-21 2020-12-25 Nanobiose Puce micro-fluidique et laboratoire sur puce-micro-fluidique
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CN110358665A (zh) * 2019-06-28 2019-10-22 马鞍山众慧惠生生物科技有限公司 箱式集成化核酸定量检测装置
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
JP7164505B2 (ja) * 2019-10-02 2022-11-01 積水化学工業株式会社 マイクロ流路チップ
WO2021231607A1 (en) * 2020-05-12 2021-11-18 Godx, Inc. Point of need diagnostic device and methods of use thereof
WO2021236850A1 (en) * 2020-05-19 2021-11-25 Mammoth Biosciences, Inc. Devices, assays and methods for detection of nucleic acids
JPWO2022030605A1 (enExample) * 2020-08-07 2022-02-10
US11567071B2 (en) 2020-10-16 2023-01-31 Fresenius Medical Care Holdings, Inc. Devices and methods for lateral flow tests of bodily fluids
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Also Published As

Publication number Publication date
ES2712666T3 (es) 2019-05-14
EP2496351A2 (en) 2012-09-12
CN102655938B (zh) 2014-09-17
TR201900390T4 (tr) 2019-02-21
JP2013509173A (ja) 2013-03-14
US9163279B2 (en) 2015-10-20
WO2011051735A2 (en) 2011-05-05
CA2778329A1 (en) 2011-05-05
EP2496351B1 (en) 2019-01-02
AU2010311138A1 (en) 2012-05-03
AU2010311138B2 (en) 2014-08-28
US20120270225A1 (en) 2012-10-25
JP5934100B2 (ja) 2016-06-15
GB0919159D0 (en) 2009-12-16
CN102655938A (zh) 2012-09-05
WO2011051735A3 (en) 2011-08-18

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Effective date: 20151029