CA2775093A1 - Down-regulation of acc synthase for improved plant performance - Google Patents
Down-regulation of acc synthase for improved plant performance Download PDFInfo
- Publication number
- CA2775093A1 CA2775093A1 CA2775093A CA2775093A CA2775093A1 CA 2775093 A1 CA2775093 A1 CA 2775093A1 CA 2775093 A CA2775093 A CA 2775093A CA 2775093 A CA2775093 A CA 2775093A CA 2775093 A1 CA2775093 A1 CA 2775093A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- expression
- seq
- plants
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000003828 downregulation Effects 0.000 title claims abstract description 63
- 230000001976 improved effect Effects 0.000 title abstract description 30
- 101710194665 1-aminocyclopropane-1-carboxylate synthase Proteins 0.000 claims abstract description 175
- 238000000034 method Methods 0.000 claims abstract description 150
- 230000009261 transgenic effect Effects 0.000 claims abstract description 70
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims abstract description 55
- 239000005977 Ethylene Substances 0.000 claims abstract description 55
- 238000004519 manufacturing process Methods 0.000 claims abstract description 41
- 230000001965 increasing effect Effects 0.000 claims abstract description 38
- 230000009467 reduction Effects 0.000 claims abstract description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 21
- 239000002028 Biomass Substances 0.000 claims abstract description 17
- 230000014075 nitrogen utilization Effects 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims description 547
- 230000014509 gene expression Effects 0.000 claims description 209
- 108090000623 proteins and genes Proteins 0.000 claims description 163
- 150000007523 nucleic acids Chemical class 0.000 claims description 128
- 102000040430 polynucleotide Human genes 0.000 claims description 116
- 108091033319 polynucleotide Proteins 0.000 claims description 116
- 239000002157 polynucleotide Substances 0.000 claims description 116
- 102000039446 nucleic acids Human genes 0.000 claims description 103
- 108020004707 nucleic acids Proteins 0.000 claims description 103
- 240000008042 Zea mays Species 0.000 claims description 92
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 73
- 230000000694 effects Effects 0.000 claims description 67
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 63
- 235000009973 maize Nutrition 0.000 claims description 63
- 239000002773 nucleotide Substances 0.000 claims description 41
- 125000003729 nucleotide group Chemical group 0.000 claims description 41
- 101100433501 Arabidopsis thaliana ACS6 gene Proteins 0.000 claims description 32
- 230000024346 drought recovery Effects 0.000 claims description 32
- 230000002829 reductive effect Effects 0.000 claims description 28
- 230000009368 gene silencing by RNA Effects 0.000 claims description 19
- 240000007594 Oryza sativa Species 0.000 claims description 18
- 235000007164 Oryza sativa Nutrition 0.000 claims description 18
- 235000009566 rice Nutrition 0.000 claims description 16
- 244000068988 Glycine max Species 0.000 claims description 15
- 235000010469 Glycine max Nutrition 0.000 claims description 14
- 108020004999 messenger RNA Proteins 0.000 claims description 14
- 241000209510 Liliopsida Species 0.000 claims description 10
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 9
- 241000209140 Triticum Species 0.000 claims description 9
- 235000021307 Triticum Nutrition 0.000 claims description 9
- 230000006870 function Effects 0.000 claims description 9
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 8
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 8
- 230000001603 reducing effect Effects 0.000 claims description 8
- 241001233957 eudicotyledons Species 0.000 claims description 7
- 244000075850 Avena orientalis Species 0.000 claims description 6
- 244000020551 Helianthus annuus Species 0.000 claims description 5
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 5
- 235000007319 Avena orientalis Nutrition 0.000 claims description 4
- 235000007238 Secale cereale Nutrition 0.000 claims description 4
- 101150047711 acs gene Proteins 0.000 claims description 4
- 235000011331 Brassica Nutrition 0.000 claims description 3
- 241000219198 Brassica Species 0.000 claims description 3
- 241001520823 Zoysia Species 0.000 claims description 3
- 230000002222 downregulating effect Effects 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims 3
- 235000007558 Avena sp Nutrition 0.000 claims 2
- 240000005979 Hordeum vulgare Species 0.000 claims 2
- 244000082988 Secale cereale Species 0.000 claims 2
- 239000013598 vector Substances 0.000 abstract description 54
- 230000036579 abiotic stress Effects 0.000 abstract description 12
- 210000004027 cell Anatomy 0.000 description 169
- 108090000765 processed proteins & peptides Proteins 0.000 description 111
- 102000004196 processed proteins & peptides Human genes 0.000 description 99
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 98
- 229920001184 polypeptide Polymers 0.000 description 97
- 210000001519 tissue Anatomy 0.000 description 69
- 108020004414 DNA Proteins 0.000 description 56
- 102000004169 proteins and genes Human genes 0.000 description 56
- 229910052757 nitrogen Inorganic materials 0.000 description 53
- 235000018102 proteins Nutrition 0.000 description 53
- 230000009466 transformation Effects 0.000 description 51
- 210000002257 embryonic structure Anatomy 0.000 description 50
- 239000002609 medium Substances 0.000 description 42
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 40
- 239000002245 particle Substances 0.000 description 37
- 230000035882 stress Effects 0.000 description 34
- 239000013612 plasmid Substances 0.000 description 32
- 235000001014 amino acid Nutrition 0.000 description 30
- 229940024606 amino acid Drugs 0.000 description 29
- 150000001413 amino acids Chemical class 0.000 description 29
- 238000009396 hybridization Methods 0.000 description 26
- 238000013518 transcription Methods 0.000 description 26
- 230000035897 transcription Effects 0.000 description 26
- 108091027967 Small hairpin RNA Proteins 0.000 description 24
- 239000000047 product Substances 0.000 description 24
- 241000589158 Agrobacterium Species 0.000 description 23
- 230000000692 anti-sense effect Effects 0.000 description 23
- 230000012010 growth Effects 0.000 description 23
- 230000008929 regeneration Effects 0.000 description 23
- 238000011069 regeneration method Methods 0.000 description 23
- 230000000295 complement effect Effects 0.000 description 21
- PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 20
- 108091026890 Coding region Proteins 0.000 description 19
- 235000013339 cereals Nutrition 0.000 description 19
- 241000894007 species Species 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
- 101000773184 Homo sapiens Twist-related protein 1 Proteins 0.000 description 17
- 230000015572 biosynthetic process Effects 0.000 description 17
- 230000001404 mediated effect Effects 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 16
- 102100034334 Fatty acid CoA ligase Acsl3 Human genes 0.000 description 16
- 101000780194 Homo sapiens Fatty acid CoA ligase Acsl3 Proteins 0.000 description 16
- 108700019146 Transgenes Proteins 0.000 description 16
- 230000000408 embryogenic effect Effects 0.000 description 16
- 239000003550 marker Substances 0.000 description 16
- 239000000203 mixture Substances 0.000 description 15
- 108020004705 Codon Proteins 0.000 description 14
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 14
- 125000003275 alpha amino acid group Chemical group 0.000 description 14
- 238000011161 development Methods 0.000 description 14
- 239000013604 expression vector Substances 0.000 description 14
- 238000006467 substitution reaction Methods 0.000 description 14
- 206010020649 Hyperkeratosis Diseases 0.000 description 13
- 230000001105 regulatory effect Effects 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 230000001629 suppression Effects 0.000 description 13
- 239000000725 suspension Substances 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 238000007792 addition Methods 0.000 description 12
- 238000003556 assay Methods 0.000 description 12
- 230000018109 developmental process Effects 0.000 description 12
- 230000002068 genetic effect Effects 0.000 description 12
- 230000001939 inductive effect Effects 0.000 description 12
- 239000007788 liquid Substances 0.000 description 12
- 210000001938 protoplast Anatomy 0.000 description 12
- 238000012546 transfer Methods 0.000 description 12
- 229910001868 water Inorganic materials 0.000 description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 11
- 244000062793 Sorghum vulgare Species 0.000 description 11
- 239000002689 soil Substances 0.000 description 11
- 238000003786 synthesis reaction Methods 0.000 description 11
- 238000013519 translation Methods 0.000 description 11
- 108700001094 Plant Genes Proteins 0.000 description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 10
- 230000004075 alteration Effects 0.000 description 10
- 238000009395 breeding Methods 0.000 description 10
- 235000005822 corn Nutrition 0.000 description 10
- 230000035772 mutation Effects 0.000 description 10
- 210000000056 organ Anatomy 0.000 description 10
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 10
- 238000003259 recombinant expression Methods 0.000 description 10
- 230000010076 replication Effects 0.000 description 10
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 9
- 230000027455 binding Effects 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 9
- 230000000392 somatic effect Effects 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 230000001131 transforming effect Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 108700010070 Codon Usage Proteins 0.000 description 8
- 241000209219 Hordeum Species 0.000 description 8
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 8
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 8
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 230000003321 amplification Effects 0.000 description 8
- 239000002585 base Substances 0.000 description 8
- 239000004009 herbicide Substances 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000012544 monitoring process Methods 0.000 description 8
- 239000000618 nitrogen fertilizer Substances 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 230000010152 pollination Effects 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 108020003589 5' Untranslated Regions Proteins 0.000 description 7
- 108010000700 Acetolactate synthase Proteins 0.000 description 7
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 7
- 125000000539 amino acid group Chemical group 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000004520 electroporation Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000008488 polyadenylation Effects 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 238000000527 sonication Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 241000219194 Arabidopsis Species 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- 241000219823 Medicago Species 0.000 description 6
- 108700026244 Open Reading Frames Proteins 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 230000008641 drought stress Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 230000001747 exhibiting effect Effects 0.000 description 6
- 230000002363 herbicidal effect Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 230000008121 plant development Effects 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000001568 sexual effect Effects 0.000 description 6
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 6
- 108091093088 Amplicon Proteins 0.000 description 5
- 241000209200 Bromus Species 0.000 description 5
- 241000701489 Cauliflower mosaic virus Species 0.000 description 5
- 229920002148 Gellan gum Polymers 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- 241000227653 Lycopersicon Species 0.000 description 5
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 5
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 108090000848 Ubiquitin Proteins 0.000 description 5
- 102000044159 Ubiquitin Human genes 0.000 description 5
- 108091023045 Untranslated Region Proteins 0.000 description 5
- 235000007244 Zea mays Nutrition 0.000 description 5
- 238000004422 calculation algorithm Methods 0.000 description 5
- 229930002875 chlorophyll Natural products 0.000 description 5
- 235000019804 chlorophyll Nutrition 0.000 description 5
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 239000010931 gold Substances 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 229930182817 methionine Natural products 0.000 description 5
- 229960004452 methionine Drugs 0.000 description 5
- 108010058731 nopaline synthase Proteins 0.000 description 5
- 238000003976 plant breeding Methods 0.000 description 5
- 235000010333 potassium nitrate Nutrition 0.000 description 5
- 239000013615 primer Substances 0.000 description 5
- 230000000644 propagated effect Effects 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000004114 suspension culture Methods 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 108010010888 1-aminocyclopropane-1-carboxylic acid oxidase Proteins 0.000 description 4
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 241001303487 Digitaria <clam> Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 244000299507 Gossypium hirsutum Species 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 241000209056 Secale Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000001110 calcium chloride Substances 0.000 description 4
- 229910001628 calcium chloride Inorganic materials 0.000 description 4
- 235000011148 calcium chloride Nutrition 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- -1 e.g. Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000011536 extraction buffer Substances 0.000 description 4
- 108020002326 glutamine synthetase Proteins 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000001307 helium Substances 0.000 description 4
- 229910052734 helium Inorganic materials 0.000 description 4
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 4
- 208000000509 infertility Diseases 0.000 description 4
- 230000036512 infertility Effects 0.000 description 4
- 208000021267 infertility disease Diseases 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- 238000007834 ligase chain reaction Methods 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 235000019713 millet Nutrition 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 238000012552 review Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- AVWQQPYHYQKEIZ-UHFFFAOYSA-K trisodium;2-dodecylbenzenesulfonate;3-dodecylbenzenesulfonate;4-dodecylbenzenesulfonate Chemical compound [Na+].[Na+].[Na+].CCCCCCCCCCCCC1=CC=C(S([O-])(=O)=O)C=C1.CCCCCCCCCCCCC1=CC=CC(S([O-])(=O)=O)=C1.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O AVWQQPYHYQKEIZ-UHFFFAOYSA-K 0.000 description 4
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 4
- 229910052721 tungsten Inorganic materials 0.000 description 4
- 239000010937 tungsten Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 3
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 3
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 3
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 3
- 235000006008 Brassica napus var napus Nutrition 0.000 description 3
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 3
- 244000188595 Brassica sinapistrum Species 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 3
- 108090000994 Catalytic RNA Proteins 0.000 description 3
- 102000053642 Catalytic RNA Human genes 0.000 description 3
- 241000218631 Coniferophyta Species 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 3
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 3
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 3
- 241000700189 Hystrix <Rodentia> Species 0.000 description 3
- 206010021929 Infertility male Diseases 0.000 description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 108091030071 RNAI Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 241000219793 Trifolium Species 0.000 description 3
- 241000219977 Vigna Species 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 235000004279 alanine Nutrition 0.000 description 3
- 101150103518 bar gene Proteins 0.000 description 3
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000011284 combination treatment Methods 0.000 description 3
- 239000003184 complementary RNA Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 235000021186 dishes Nutrition 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
- 102000005396 glutamine synthetase Human genes 0.000 description 3
- 108010002685 hygromycin-B kinase Proteins 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 108010083942 mannopine synthase Proteins 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000000520 microinjection Methods 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 210000002706 plastid Anatomy 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 210000001236 prokaryotic cell Anatomy 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 230000003938 response to stress Effects 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- 108091092562 ribozyme Proteins 0.000 description 3
- 230000021749 root development Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- 239000004460 silage Substances 0.000 description 3
- 229940063673 spermidine Drugs 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000234282 Allium Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000208838 Asteraceae Species 0.000 description 2
- 235000005781 Avena Nutrition 0.000 description 2
- 241000848284 Avenella Species 0.000 description 2
- 241000560364 Avenula Species 0.000 description 2
- 241000743774 Brachypodium Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 241001551143 Catapodium Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 241000142836 Danthonia Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000744312 Ehrharta Species 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 241000220485 Fabaceae Species 0.000 description 2
- 241000234642 Festuca Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- 241001523358 Glyceria Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 241001551113 Hainardia Species 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000044446 Melinis Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 2
- 241000320642 Monanthochloe Species 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 239000006002 Pepper Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000016761 Piper aduncum Nutrition 0.000 description 2
- 240000003889 Piper guineense Species 0.000 description 2
- 235000017804 Piper guineense Nutrition 0.000 description 2
- 235000008184 Piper nigrum Nutrition 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 241001523344 Pseudoroegneria Species 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 2
- 241001475959 Rostraria Species 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 235000007230 Sorghum bicolor Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241000052184 Swallenia Species 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 102000003929 Transaminases Human genes 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 235000010726 Vigna sinensis Nutrition 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229960001570 ademetionine Drugs 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
- 230000004790 biotic stress Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 230000004077 genetic alteration Effects 0.000 description 2
- 231100000118 genetic alteration Toxicity 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- 108010039239 glyphosate N-acetyltransferase Proteins 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 238000007826 nucleic acid assay Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 101150113864 pat gene Proteins 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000008447 perception Effects 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000008640 plant stress response Effects 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 description 2
- 229920000903 polyhydroxyalkanoate Polymers 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 229960002429 proline Drugs 0.000 description 2
- 238000002731 protein assay Methods 0.000 description 2
- 238000000164 protein isolation Methods 0.000 description 2
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 230000009758 senescence Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 230000030118 somatic embryogenesis Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 108010008664 streptomycin 3''-kinase Proteins 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000019157 thiamine Nutrition 0.000 description 2
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- 239000011721 thiamine Substances 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- 230000017105 transposition Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 101150085703 vir gene Proteins 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 241000228158 x Triticosecale Species 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- GNKZMNRKLCTJAY-UHFFFAOYSA-N 4'-Methylacetophenone Chemical compound CC(=O)C1=CC=C(C)C=C1 GNKZMNRKLCTJAY-UHFFFAOYSA-N 0.000 description 1
- WUUGFSXJNOTRMR-IOSLPCCCSA-N 5'-S-methyl-5'-thioadenosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CSC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 WUUGFSXJNOTRMR-IOSLPCCCSA-N 0.000 description 1
- 101150011812 AADAC gene Proteins 0.000 description 1
- 101150031979 ACS6 gene Proteins 0.000 description 1
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 1
- 101150001232 ALS gene Proteins 0.000 description 1
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
- 241001523383 Achnatherum Species 0.000 description 1
- 241000047962 Acroceras Species 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000209758 Aegilops Species 0.000 description 1
- 241000209136 Agropyron Species 0.000 description 1
- 241000743339 Agrostis Species 0.000 description 1
- 241000772991 Aira Species 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 108010025188 Alcohol oxidase Proteins 0.000 description 1
- 241000756998 Alismatales Species 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 241000051981 Allolepis Species 0.000 description 1
- 241001018099 Alloteropsis Species 0.000 description 1
- 241000743985 Alopecurus Species 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000209130 Amblyopyrum Species 0.000 description 1
- 241000368670 Ammophila <wasp> Species 0.000 description 1
- 241000923583 Ampelodesmos Species 0.000 description 1
- 241000070750 Amphicarpum Species 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000260674 Anthaenantia Species 0.000 description 1
- 241001400082 Anthephora Species 0.000 description 1
- 241000743857 Anthoxanthum Species 0.000 description 1
- 241000207875 Antirrhinum Species 0.000 description 1
- 241001666377 Apera Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 241000306749 Apluda Species 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 101100433502 Arabidopsis thaliana ACS7 gene Proteins 0.000 description 1
- 101100382386 Arabidopsis thaliana PPC3 gene Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000316756 Arctophila Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241001520919 Aristida Species 0.000 description 1
- 241000508787 Arrhenatherum Species 0.000 description 1
- 241000772998 Arthraxon Species 0.000 description 1
- 241000772989 Arthrostylidium Species 0.000 description 1
- 241000209134 Arundinaria Species 0.000 description 1
- 241000931363 Arundinella Species 0.000 description 1
- 241001494510 Arundo Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241001106067 Atropa Species 0.000 description 1
- 241000047982 Axonopus Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241000209128 Bambusa Species 0.000 description 1
- 241001447692 Beckmannia Species 0.000 description 1
- 241000051984 Blepharidachne Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241001018635 Borinda Species 0.000 description 1
- 241001327393 Bothriochloa Species 0.000 description 1
- 241000232312 Bouteloua Species 0.000 description 1
- 241001135655 Bouteloua erecta Species 0.000 description 1
- 241000611157 Brachiaria Species 0.000 description 1
- 241000339490 Brachyachne Species 0.000 description 1
- 241001520110 Brachyelytrum Species 0.000 description 1
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 241000743778 Briza Species 0.000 description 1
- 241000234670 Bromeliaceae Species 0.000 description 1
- 241000320719 Buchloe Species 0.000 description 1
- 241000743799 Calamagrostis Species 0.000 description 1
- 241000743801 Calamovilfa Species 0.000 description 1
- 101100507655 Canis lupus familiaris HSPA1 gene Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- 235000009025 Carya illinoensis Nutrition 0.000 description 1
- 244000068645 Carya illinoensis Species 0.000 description 1
- 241001490227 Catabrosa Species 0.000 description 1
- 241000209120 Cenchrus Species 0.000 description 1
- 241000931368 Centotheca Species 0.000 description 1
- 241001520886 Chasmanthium Species 0.000 description 1
- 241001520833 Chimonobambusa Species 0.000 description 1
- 241000593460 Chionochloa Species 0.000 description 1
- 241000861718 Chloris <Aves> Species 0.000 description 1
- 239000005496 Chlorsulfuron Substances 0.000 description 1
- 241001494513 Chusquea Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 241000744472 Cinna Species 0.000 description 1
- 108010061190 Cinnamyl-alcohol dehydrogenase Proteins 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000408178 Cladoraphis Species 0.000 description 1
- 241000306777 Coelorachis Species 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 241000209205 Coix Species 0.000 description 1
- 241000507380 Coleanthus Species 0.000 description 1
- 241000019460 Colpodium Species 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 241000217270 Cornucopiae Species 0.000 description 1
- 241000593305 Cortaderia Species 0.000 description 1
- 240000009226 Corylus americana Species 0.000 description 1
- 235000001543 Corylus americana Nutrition 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 241000560213 Corynephorus Species 0.000 description 1
- 241000320752 Cottea Species 0.000 description 1
- 241001523387 Critesion Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- 241001018651 Crypsis Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000052019 Ctenium Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 244000024469 Cucumis prophetarum Species 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 241001144051 Cutandia Species 0.000 description 1
- 241000931332 Cymbopogon Species 0.000 description 1
- FEPOUSPSESUQPD-UHFFFAOYSA-N Cymbopogon Natural products C1CC2(C)C(C)C(=O)CCC2C2(C)C1C1(C)CCC3(C)CCC(C)C(C)C3C1(C)CC2 FEPOUSPSESUQPD-UHFFFAOYSA-N 0.000 description 1
- 244000052363 Cynodon dactylon Species 0.000 description 1
- 241001551134 Cynosurus Species 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 101710096830 DNA-3-methyladenine glycosylase Proteins 0.000 description 1
- 102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 241000209210 Dactylis Species 0.000 description 1
- 241000320605 Dactyloctenium Species 0.000 description 1
- 241000052043 Dasyochloa Species 0.000 description 1
- 241000208296 Datura Species 0.000 description 1
- 241000208175 Daucus Species 0.000 description 1
- 235000013262 Dendrocalamus Nutrition 0.000 description 1
- 241000744361 Dendrocalamus Species 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 241000848289 Desmazeria Species 0.000 description 1
- 241001163087 Deyeuxia Species 0.000 description 1
- 241001520003 Diarrhena Species 0.000 description 1
- 241000338062 Dichanthelium Species 0.000 description 1
- 241001327308 Dichanthium Species 0.000 description 1
- 241001163054 Dichelachne Species 0.000 description 1
- 240000001879 Digitalis lutea Species 0.000 description 1
- 235000017896 Digitaria Nutrition 0.000 description 1
- 241001433320 Dimeria Species 0.000 description 1
- 241000145742 Dinebra Species 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 241000320596 Diplachne Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001490199 Dissanthelium Species 0.000 description 1
- 241000070810 Dissochondrus Species 0.000 description 1
- 241001306223 Drepanostachyum Species 0.000 description 1
- 241000316785 Dupontia Species 0.000 description 1
- 241000192043 Echinochloa Species 0.000 description 1
- 241000209215 Eleusine Species 0.000 description 1
- 235000007351 Eleusine Nutrition 0.000 description 1
- 241000142841 Enneapogon Species 0.000 description 1
- 101710198510 Enoyl-[acyl-carrier-protein] reductase [NADH] Proteins 0.000 description 1
- 241000145748 Enteropogon <grass> Species 0.000 description 1
- 241001518935 Eragrostis Species 0.000 description 1
- 241001433310 Eremochloa Species 0.000 description 1
- 241001490176 Eremopoa Species 0.000 description 1
- 241001523362 Eremopyrum Species 0.000 description 1
- 241000606889 Eriochrysis Species 0.000 description 1
- 241000052079 Erioneuron Species 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 241001585085 Euchlaena Species 0.000 description 1
- 241001606303 Euclasta <moth> Species 0.000 description 1
- 241000945868 Eulaliopsis Species 0.000 description 1
- 241001520106 Eustachys Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000161991 Fargesia Species 0.000 description 1
- 108010087894 Fatty acid desaturases Proteins 0.000 description 1
- 241000032244 Festuca subg. Vulpia Species 0.000 description 1
- 241000145757 Fingerhuthia Species 0.000 description 1
- 241000220223 Fragaria Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241001414529 Garnotia Species 0.000 description 1
- 241001476001 Gastridium <grass> Species 0.000 description 1
- 241000560209 Gaudinia Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 235000003508 Gigantochloa thoii Nutrition 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 235000000512 Glyceria Nutrition 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241001476003 Graphephorum Species 0.000 description 1
- 241000320721 Gymnopogon Species 0.000 description 1
- 241000142762 Gynerium Species 0.000 description 1
- 241000744834 Hakonechloa Species 0.000 description 1
- 241000122202 Haynaldia Species 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 241000245622 Helictotrichon Species 0.000 description 1
- 241000931313 Hemarthria Species 0.000 description 1
- 241000270901 Hesperostipa Species 0.000 description 1
- 241001582739 Heteropogon <robber fly> Species 0.000 description 1
- 241000773457 Hibanobambusa Species 0.000 description 1
- 241000825107 Hierochloe Species 0.000 description 1
- 241000052086 Hilaria Species 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 241000744855 Holcus Species 0.000 description 1
- 101000780205 Homo sapiens Long-chain-fatty-acid-CoA ligase 5 Proteins 0.000 description 1
- 101000780202 Homo sapiens Long-chain-fatty-acid-CoA ligase 6 Proteins 0.000 description 1
- 101000582320 Homo sapiens Neurogenic differentiation factor 6 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 241000044414 Hymenachne Species 0.000 description 1
- 241000208278 Hyoscyamus Species 0.000 description 1
- 244000265274 Hyparrhenia filipendula Species 0.000 description 1
- 241000044417 Ichnanthus Species 0.000 description 1
- 240000007171 Imperata cylindrica Species 0.000 description 1
- 241000755540 Indocalamus Species 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 240000006392 Isachne Species 0.000 description 1
- 241001327265 Ischaemum Species 0.000 description 1
- 241000496998 Ixophorus Species 0.000 description 1
- 241000758789 Juglans Species 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 241000191948 Kocuria rosea Species 0.000 description 1
- 241000245643 Koeleria Species 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000208822 Lactuca Species 0.000 description 1
- 241001444143 Lagurus <angiosperm> Species 0.000 description 1
- 241000744787 Lamarckia Species 0.000 description 1
- 241000044443 Lasiacis Species 0.000 description 1
- 240000006568 Lathyrus odoratus Species 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 241001494496 Leersia Species 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 241000320639 Leptochloa Species 0.000 description 1
- 241000145637 Lepturus Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000511731 Leymus Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 241000234269 Liliales Species 0.000 description 1
- 241001497103 Limnodea Species 0.000 description 1
- 241000208204 Linum Species 0.000 description 1
- 241001494498 Lithachne Species 0.000 description 1
- 102100034318 Long-chain-fatty-acid-CoA ligase 5 Human genes 0.000 description 1
- 241001421529 Lophopyrum Species 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 241001484124 Luziola Species 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- 241000745397 Lygeum Species 0.000 description 1
- 241000121629 Majorana Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 241001507457 Manisuris Species 0.000 description 1
- 241001329086 Megastachya Species 0.000 description 1
- 241000213996 Melilotus Species 0.000 description 1
- 235000000839 Melilotus officinalis subsp suaveolens Nutrition 0.000 description 1
- 101100409013 Mesembryanthemum crystallinum PPD gene Proteins 0.000 description 1
- 241000560223 Mibora Species 0.000 description 1
- 241000526657 Microchloa Species 0.000 description 1
- 241000306779 Microstegium Species 0.000 description 1
- 241001490213 Milium Species 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 241000878006 Miscanthus sinensis Species 0.000 description 1
- 241001401380 Mnesithea Species 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 241001520029 Molinia Species 0.000 description 1
- 244000111261 Mucuna pruriens Species 0.000 description 1
- 235000008540 Mucuna pruriens var utilis Nutrition 0.000 description 1
- 241000592161 Muhlenbergia Species 0.000 description 1
- 241000052145 Munroa Species 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 241000204025 Mycoplasma capricolum Species 0.000 description 1
- 229910018890 NaMoO4 Inorganic materials 0.000 description 1
- 241000745334 Nardus Species 0.000 description 1
- 241001230286 Narenga Species 0.000 description 1
- 241001523369 Nassella Species 0.000 description 1
- 241001282315 Nemesis Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000495186 Neoschischkinia Species 0.000 description 1
- 241000320703 Neostapfia Species 0.000 description 1
- 102100030589 Neurogenic differentiation factor 6 Human genes 0.000 description 1
- 241001018646 Neyraudia Species 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 241000208133 Nicotiana plumbaginifolia Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 241001520828 Olyra <angiosperm> Species 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 241000931244 Oplismenus Species 0.000 description 1
- 241000145654 Orcuttia Species 0.000 description 1
- 241001518636 Oryzopsis Species 0.000 description 1
- 241000216616 Otatea Species 0.000 description 1
- 241001645266 Oxytenanthera Species 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 235000001591 Pachyrhizus erosus Nutrition 0.000 description 1
- 244000258470 Pachyrhizus tuberosus Species 0.000 description 1
- 235000018669 Pachyrhizus tuberosus Nutrition 0.000 description 1
- 241000209117 Panicum Species 0.000 description 1
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 1
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 1
- 241000145659 Pappophorum Species 0.000 description 1
- 241001550949 Parapholis Species 0.000 description 1
- 206010034016 Paronychia Diseases 0.000 description 1
- 241001668545 Pascopyrum Species 0.000 description 1
- 241000044524 Paspalidium Species 0.000 description 1
- 241001330453 Paspalum Species 0.000 description 1
- 241000208181 Pelargonium Species 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 241000745991 Phalaris Species 0.000 description 1
- 241000226265 Phanopyrum Species 0.000 description 1
- 241000829202 Pharus <Mollusca> Species 0.000 description 1
- 241000219833 Phaseolus Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 101000870887 Phaseolus vulgaris Glycine-rich cell wall structural protein 1.8 Proteins 0.000 description 1
- 241001490218 Phippsia Species 0.000 description 1
- 241000746981 Phleum Species 0.000 description 1
- 241001401260 Pholiurus Species 0.000 description 1
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 235000005205 Pinus Nutrition 0.000 description 1
- 241000218602 Pinus <genus> Species 0.000 description 1
- 241000508729 Piptatherum Species 0.000 description 1
- 241001523341 Piptochaetium Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 241000400666 Pleioblastus Species 0.000 description 1
- 241000145176 Pleuraphis Species 0.000 description 1
- 241000635397 Pleuropogon Species 0.000 description 1
- 241000209048 Poa Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000848268 Podagrostis Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920000331 Polyhydroxybutyrate Polymers 0.000 description 1
- 241000724416 Polypogon <angiosperm> Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241001433241 Polytrias Species 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000209122 Psathyrostachys Species 0.000 description 1
- 241000216609 Pseudosasa Species 0.000 description 1
- 241000270678 Ptilagrostis Species 0.000 description 1
- 241000736229 Puccinellia Species 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 108020005161 RNA Caps Proteins 0.000 description 1
- 108020005067 RNA Splice Sites Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 101150075111 ROLB gene Proteins 0.000 description 1
- 241000218206 Ranunculus Species 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 241000073276 Reimarochloa Species 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000857233 Rottboellia Species 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000209051 Saccharum Species 0.000 description 1
- 241000044558 Sacciolepis Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 240000005499 Sasa Species 0.000 description 1
- 241000267039 Sasaella Species 0.000 description 1
- 241000611425 Schismus Species 0.000 description 1
- 241000147395 Schizachne Species 0.000 description 1
- 241001327272 Schizachyrium Species 0.000 description 1
- 241000216509 Schizostachyum Species 0.000 description 1
- 241001550934 Sclerochloa Species 0.000 description 1
- 241000052179 Scleropogon <grass> Species 0.000 description 1
- 241000158927 Scolochloa Species 0.000 description 1
- 241000319026 Scribneria Species 0.000 description 1
- 241000543808 Semiarundinaria Species 0.000 description 1
- 241000780602 Senecio Species 0.000 description 1
- 241001116459 Sequoia Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000508743 Sesleria Species 0.000 description 1
- 235000005775 Setaria Nutrition 0.000 description 1
- 241000232088 Setaria <nematode> Species 0.000 description 1
- 241000498786 Shibataea Species 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- 241000543826 Sinobambusa Species 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 101000915835 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate synthase 3 Proteins 0.000 description 1
- 241001327268 Sorghastrum Species 0.000 description 1
- 101000815956 Sorghum bicolor DNA-directed RNA polymerase subunit alpha Proteins 0.000 description 1
- 101000727343 Sorghum bicolor DNA-directed RNA polymerase subunit beta Proteins 0.000 description 1
- 101001088501 Sorghum bicolor DNA-directed RNA polymerase subunit beta' Proteins 0.000 description 1
- 101000649260 Sorghum bicolor DNA-directed RNA polymerase subunit beta'' Proteins 0.000 description 1
- 241000746413 Spartina Species 0.000 description 1
- 241000560237 Sphenopholis Species 0.000 description 1
- 241001140642 Spodiopogon Species 0.000 description 1
- 241001520881 Sporobolus Species 0.000 description 1
- 101100289792 Squirrel monkey polyomavirus large T gene Proteins 0.000 description 1
- 108010039811 Starch synthase Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000044574 Steinchisma Species 0.000 description 1
- 244000087212 Stenotaphrum Species 0.000 description 1
- 241000746422 Stipa Species 0.000 description 1
- 241000142724 Stipagrostis Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241001523334 Taeniatherum Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241001306213 Thamnocalamus Species 0.000 description 1
- 241000931197 Themeda Species 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 101710194092 Thiamine-phosphate synthase 1 Proteins 0.000 description 1
- 241000209124 Thinopyrum Species 0.000 description 1
- 102000002933 Thioredoxin Human genes 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 241001208278 Thuarea Species 0.000 description 1
- 241001520811 Thysanolaena Species 0.000 description 1
- 241000848292 Torreyochloa Species 0.000 description 1
- 241000592342 Tracheophyta Species 0.000 description 1
- 241001528284 Trachypogon Species 0.000 description 1
- 241000746998 Tragus Species 0.000 description 1
- 241000145178 Trichloris Species 0.000 description 1
- 241000746999 Tricholaena Species 0.000 description 1
- 241000145171 Trichoneura Species 0.000 description 1
- 241000145122 Tridens <angiosperm> Species 0.000 description 1
- 241001312519 Trigonella Species 0.000 description 1
- 241000931213 Triodia <angiosperm> Species 0.000 description 1
- 241000052209 Triplasis Species 0.000 description 1
- 241000052213 Tripogon Species 0.000 description 1
- 241000209138 Tripsacum Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 241000005601 Trisetum Species 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 241000320679 Tuctoria Species 0.000 description 1
- 241000145124 Uniola Species 0.000 description 1
- 241001521331 Urochloa Species 0.000 description 1
- 241000372906 Vahlodea Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000320760 Vaseyochloa Species 0.000 description 1
- 241000172367 Ventenata Species 0.000 description 1
- 241000414043 Vetiveria Species 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 241000052229 Willkommia Species 0.000 description 1
- 241000219995 Wisteria Species 0.000 description 1
- 241001523432 Zale Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 241000746966 Zizania Species 0.000 description 1
- 241001478413 Zizaniopsis Species 0.000 description 1
- 240000001102 Zoysia matrella Species 0.000 description 1
- FHHZHGZBHYYWTG-INFSMZHSSA-N [(2r,3s,4r,5r)-5-(2-amino-7-methyl-6-oxo-3h-purin-9-ium-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl [[[(2r,3s,4r,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl] phosphate Chemical compound N1C(N)=NC(=O)C2=C1[N+]([C@H]1[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=C(C(N=C(N)N4)=O)N=C3)O)O1)O)=CN2C FHHZHGZBHYYWTG-INFSMZHSSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000006578 abscission Effects 0.000 description 1
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 244000193174 agave Species 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- CSGLCWIAEFNDIL-UHFFFAOYSA-O azanium;urea;nitrate Chemical compound [NH4+].NC(N)=O.[O-][N+]([O-])=O CSGLCWIAEFNDIL-UHFFFAOYSA-O 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000010310 bacterial transformation Effects 0.000 description 1
- 108010021384 barley lectin Proteins 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 102000006635 beta-lactamase Human genes 0.000 description 1
- 229910002056 binary alloy Inorganic materials 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- HOZOZZFCZRXYEK-GSWUYBTGSA-M butylscopolamine bromide Chemical compound [Br-].C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3[N+]([C@H](C2)[C@@H]2[C@H]3O2)(C)CCCC)=CC=CC=C1 HOZOZZFCZRXYEK-GSWUYBTGSA-M 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 101150039352 can gene Proteins 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 108010040093 cellulose synthase Proteins 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 108010069282 cis-prenyl transferase Proteins 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 229910001429 cobalt ion Inorganic materials 0.000 description 1
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 1
- 235000016213 coffee Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 230000008645 cold stress Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004883 computer application Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 108010065744 ethylene forming enzyme Proteins 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 239000003008 fumonisin Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000011890 leaf development Effects 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 210000002231 macronucleus Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000020429 meristem development Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 230000005305 organ development Effects 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000000596 photon cross correlation spectroscopy Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000008119 pollen development Effects 0.000 description 1
- 239000005015 poly(hydroxybutyrate) Substances 0.000 description 1
- 229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 101150063097 ppdK gene Proteins 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 1
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 1
- 229960001327 pyridoxal phosphate Drugs 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 238000012755 real-time RT-PCR analysis Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000011869 shoot development Effects 0.000 description 1
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 1
- 229910010271 silicon carbide Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000000707 stereoselective effect Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 230000006032 tissue transformation Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 244000034968 watho Species 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 241000110300 x Agropogon Species 0.000 description 1
- 241000441614 x Festulolium Species 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24806009P | 2009-10-02 | 2009-10-02 | |
| US61/248,060 | 2009-10-02 | ||
| US29090209P | 2009-12-30 | 2009-12-30 | |
| US61/290,902 | 2009-12-30 | ||
| US33206910P | 2010-05-06 | 2010-05-06 | |
| US61/332,069 | 2010-05-06 | ||
| PCT/US2010/051358 WO2011041796A1 (en) | 2009-10-02 | 2010-10-04 | Down-regulation of acc synthase for improved plant performance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2775093A1 true CA2775093A1 (en) | 2011-04-07 |
Family
ID=43566754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2775093A Abandoned CA2775093A1 (en) | 2009-10-02 | 2010-10-04 | Down-regulation of acc synthase for improved plant performance |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20110252505A1 (forum.php) |
| EP (1) | EP2483404A1 (forum.php) |
| CN (1) | CN102597229A (forum.php) |
| AR (1) | AR078502A1 (forum.php) |
| BR (1) | BR112012007516A2 (forum.php) |
| CA (1) | CA2775093A1 (forum.php) |
| IN (1) | IN2012DN02408A (forum.php) |
| MX (1) | MX2012003972A (forum.php) |
| WO (1) | WO2011041796A1 (forum.php) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102395674B (zh) * | 2009-04-14 | 2015-07-29 | 先锋国际良种公司 | 调节acc合酶改善低氮条件下的植物产量 |
| CN102732528A (zh) * | 2011-04-15 | 2012-10-17 | 华中农业大学 | Oxhs4基因在控制水稻抗旱性中的应用 |
| CN106591354B (zh) * | 2016-12-02 | 2019-08-23 | 武汉生物工程学院 | 氨基酸转运基因OsAAP5在水稻选育中的应用 |
| US10378459B2 (en) * | 2017-03-23 | 2019-08-13 | Ford Global Technologies, Llc | Method and system for engine control |
| CN110199877A (zh) * | 2019-07-04 | 2019-09-06 | 安徽省农业科学院棉花研究所 | 一种通过小孢子培养获得表型稳定的纯合薏苡资源的方法 |
Family Cites Families (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US542901A (en) | 1895-07-16 | Cabinet | ||
| US4658082A (en) | 1984-07-25 | 1987-04-14 | Atlantic Richfield Company | Method for producing intact plants containing foreign DNA |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DE3765449D1 (de) | 1986-03-11 | 1990-11-15 | Plant Genetic Systems Nv | Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen. |
| US5453566A (en) | 1986-03-28 | 1995-09-26 | Calgene, Inc. | Antisense regulation of gene expression in plant/cells |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| JPH0293088A (ja) | 1988-09-29 | 1990-04-03 | Permelec Electrode Ltd | 水電解方法及び装置 |
| US5023179A (en) | 1988-11-14 | 1991-06-11 | Eric Lam | Promoter enhancer element for gene expression in plant roots |
| US5110732A (en) | 1989-03-14 | 1992-05-05 | The Rockefeller University | Selective gene expression in plants |
| US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5034323A (en) | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5262306A (en) | 1989-09-26 | 1993-11-16 | Robeson David J | Methods for identifying cercosporin-degrading microorganisms |
| CA2074355C (en) | 1990-01-22 | 2008-10-28 | Ronald C. Lundquist | Method of producing fertile transgenic corn plants |
| PL295547A1 (forum.php) | 1990-04-26 | 1992-10-05 | Plant Genetic Systems Nv | |
| US5706603A (en) | 1990-11-16 | 1998-01-13 | E. I. Du Pont De Nemours And Company | Production method for corn with enhanced quality grain traits |
| US5704160A (en) | 1990-11-16 | 1998-01-06 | E. I. Du Pont De Nemours And Company | Production method for high-oil corn grain |
| US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
| AU675628B2 (en) | 1991-08-02 | 1997-02-13 | Sumitomo Chemical Company, Limited | Novel microorganism and insecticide |
| ATE276373T1 (de) | 1991-10-04 | 2004-10-15 | Univ North Carolina State | Pathogenresistente transgene pflanzen |
| JPH07501701A (ja) | 1991-12-04 | 1995-02-23 | イー・アイ・デユポン・ドウ・ヌムール・アンド・カンパニー | 植物由来の脂肪酸不飽和化酵素遺伝子 |
| US5341001A (en) | 1992-02-13 | 1994-08-23 | Matsushita Electric Industrial Co., Ltd. | Sulfide-selenide manganese-zinc mixed crystal photo semiconductor and laser diode |
| GB9210273D0 (en) | 1992-05-13 | 1992-07-01 | Ici Plc | Dna |
| EP0604662B1 (en) | 1992-07-07 | 2008-06-18 | Japan Tobacco Inc. | Method of transforming monocotyledon |
| ES2198408T3 (es) | 1992-11-17 | 2004-02-01 | E.I. Du Pont De Nemours And Company | Genes para delta 12 desaturasas de acidos grasos microsomales y enzimas afines de plantas. |
| WO1994012014A1 (en) | 1992-11-20 | 1994-06-09 | Agracetus, Inc. | Transgenic cotton plants producing heterologous bioplastic |
| WO1994016078A2 (en) | 1993-01-13 | 1994-07-21 | Pioneer Hi-Bred International, Inc. | High lysine derivatives of alpha-hordothionin |
| US5583210A (en) | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
| DK0672752T3 (da) | 1993-09-03 | 2004-10-04 | Japan Tobacco Inc | Fremgangsmåde til transformering af monocotyledoner ved anvendelse af scutella af uudviklede embryoner |
| US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
| US5470353A (en) | 1993-10-20 | 1995-11-28 | Hollister Incorporated | Post-operative thermal blanket |
| CA2178729A1 (en) | 1993-12-09 | 1995-06-15 | Eric B. Kmiec | Compounds and methods for site-directed mutations in eukaryotic cells |
| JPH07177130A (ja) | 1993-12-21 | 1995-07-14 | Fujitsu Ltd | エラーカウント回路 |
| US5559252A (en) | 1994-02-16 | 1996-09-24 | The Dupont Merck Pharmaceutical Company | Process for the preparation of diamine intermediates useful in the synthesis of HIV protease inhibitors |
| US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
| US5633363A (en) | 1994-06-03 | 1997-05-27 | Iowa State University, Research Foundation In | Root preferential promoter |
| US5501836A (en) | 1994-07-11 | 1996-03-26 | Hewlett Packard Company | Entrapped non-enzymatic macromolecules for chemical sensing |
| US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
| US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
| US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| MX9709352A (es) | 1995-06-02 | 1998-02-28 | Pioneer Hi Bred Int | Derivados de alfa-hortodionina con alto contenido de metionina. |
| HUP9900876A2 (hu) | 1995-06-02 | 1999-07-28 | Pioneer Hi-Bred International, Inc. | Az alfa-hordotionin nagy treonintartalmú származékai |
| US5837876A (en) | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
| US5737514A (en) | 1995-11-29 | 1998-04-07 | Texas Micro, Inc. | Remote checkpoint memory system and protocol for fault-tolerant computer system |
| US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
| US5760012A (en) | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
| US5731181A (en) | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
| AU3495297A (en) | 1996-07-08 | 1998-02-02 | Pioneer Hi-Bred International, Inc. | Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs |
| US5824875A (en) * | 1996-10-01 | 1998-10-20 | Colorado State University Through Its Agent Colorado State University Research Foundation | 1-aminocyclopropane-1-carboxylate synthase genes from pelargonium |
| JP3441899B2 (ja) | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長ｃＤＮＡライブラリーの作成方法 |
| US6232529B1 (en) | 1996-11-20 | 2001-05-15 | Pioneer Hi-Bred International, Inc. | Methods of producing high-oil seed by modification of starch levels |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| GB9703146D0 (en) | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
| JP2002511851A (ja) | 1997-04-30 | 2002-04-16 | リージェンツ オブ ジ ユニバーシティー オブ ミネソタ | 肝細胞の遺伝的病変を修正するためのリコンビナジェニックオリゴヌクレオ塩基のｉｎ ｖｉｖｏでの使用 |
| GB9710475D0 (en) | 1997-05-21 | 1997-07-16 | Zeneca Ltd | Gene silencing |
| CN100419083C (zh) | 1997-08-05 | 2008-09-17 | 金默拉根有限公司 | 用混合双链寡核苷酸在植物中实现定点遗传改变的用途 |
| CA2305866A1 (en) | 1997-11-18 | 1999-05-27 | Pioneer Hi-Bred International, Inc. | A method for directional stable transformation of eukaryotic cells |
| FR2772047B1 (fr) | 1997-12-05 | 2004-04-09 | Ct Nat D Etudes Veterinaires E | Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection |
| ATE526405T1 (de) | 1998-03-20 | 2011-10-15 | Commw Scient Ind Res Org | Synthetische gene und genetische zusammensetzungen diesen enthaltend |
| PT1068311E (pt) | 1998-04-08 | 2011-07-20 | Commw Scient Ind Res Org | Processos e meios de obter fenótipos modificados |
| EP1080197A2 (en) | 1998-05-22 | 2001-03-07 | Pioneer Hi-Bred International, Inc. | Cell cycle genes, proteins and uses thereof |
| AR020078A1 (es) | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| HUP0103556A3 (en) | 1998-08-17 | 2003-07-28 | Pioneer Hi Bred Int | Maize cellulose synthases and uses thereof |
| US7307149B2 (en) | 1998-08-17 | 2007-12-11 | Pioneer Hi-Bred International, Inc. | Maize cellulose synthases and uses thereof |
| US6518487B1 (en) | 1998-09-23 | 2003-02-11 | Pioneer Hi-Bred International, Inc. | Cyclin D polynucleotides, polypeptides and uses thereof |
| US6504083B1 (en) | 1998-10-06 | 2003-01-07 | Pioneer Hi-Bred International, Inc. | Maize Gos-2 promoters |
| AU3369900A (en) | 1999-02-19 | 2000-09-04 | General Hospital Corporation, The | Gene silencing |
| US6992237B1 (en) | 1999-04-16 | 2006-01-31 | Pioneer Hi-Bred International Inc. | Regulated expression of genes in plant seeds |
| US7531723B2 (en) | 1999-04-16 | 2009-05-12 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
| US6645747B1 (en) | 1999-09-21 | 2003-11-11 | E. I. Du Pont De Nemours And Company | Cis-prenyltransferases from plants |
| CA2391879C (en) | 1999-11-17 | 2006-04-25 | Pioneer Hi-Bred International, Inc. | Modulation of plant response to abscisic acid |
| WO2001096580A2 (en) | 2000-06-16 | 2001-12-20 | Thomas Schmulling | Method for modifying plant morphology, biochemistry and physiology using cytokinin oxidases |
| EP1297163B1 (en) | 2000-06-23 | 2011-04-27 | E.I. Du Pont De Nemours And Company | Recombinant constructs and their use in reducing gene expression |
| US20020048814A1 (en) | 2000-08-15 | 2002-04-25 | Dna Plant Technology Corporation | Methods of gene silencing using poly-dT sequences |
| US7462481B2 (en) | 2000-10-30 | 2008-12-09 | Verdia, Inc. | Glyphosate N-acetyltransferase (GAT) genes |
| EP1414980A2 (en) * | 2001-07-10 | 2004-05-06 | Universite De Montreal | Plant transcriptional repressor, proteic nuclear factors binding thereto, and uses thereof |
| BR0308424A (pt) | 2002-03-14 | 2005-02-22 | Commw Scient Ind Res Org | Métodos e meios para infra-regular eficientemente a expressão de qualquer gene de interesse em células e organismos eucarióticos |
| US20040123347A1 (en) | 2002-12-20 | 2004-06-24 | Hinchey Brendan S. | Water-deficit-inducible plant promoters |
| US7279615B2 (en) | 2003-06-16 | 2007-10-09 | Pioneer Hi-Bred International, Inc. | Floral transition genes in maize and uses thereof |
| US7230161B2 (en) * | 2003-06-23 | 2007-06-12 | Pioneer Hi-Bred International, Inc. | Engineering single-gene-controlled staygreen potential into plants utilizing ACC synthase from maize |
| US7411112B2 (en) | 2003-10-09 | 2008-08-12 | Pioneer Hi-Bred International, Inc. | Maize promoter named CRWAQ81 |
| US7405074B2 (en) | 2004-04-29 | 2008-07-29 | Pioneer Hi-Bred International, Inc. | Glyphosate-N-acetyltransferase (GAT) genes |
| US20060064786A1 (en) | 2004-09-17 | 2006-03-23 | Pioneer Hi-Bred International, Inc. | Isopentenyl transferase sequences and methods of use |
| NZ568867A (en) | 2005-08-24 | 2010-12-24 | Pioneer Hi Bred Int | Compositions providing tolerance to multiple herbicides and methods of use thereof |
| US20090229014A1 (en) | 2006-04-07 | 2009-09-10 | Xing Wang Deng | Drought and High Salinity Stress Induced Rice Promoters |
| CA2651177C (en) | 2006-05-03 | 2016-11-22 | Plexus Biomedical, Inc. | Apparatus and method of inhibiting perianal tissue damage |
| CN101939331B (zh) | 2007-08-07 | 2014-01-15 | 先锋高级育种国际公司 | 指导玉米胚珠和授粉籽粒的母体和支持组织中转基因表达的植物调节区 |
| CA2727598A1 (en) | 2008-06-10 | 2009-12-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods of use of mitogen-activated protein kinase kinase kinase |
| CN102395674B (zh) * | 2009-04-14 | 2015-07-29 | 先锋国际良种公司 | 调节acc合酶改善低氮条件下的植物产量 |
-
2010
- 2010-10-04 MX MX2012003972A patent/MX2012003972A/es not_active Application Discontinuation
- 2010-10-04 CN CN2010800434674A patent/CN102597229A/zh active Pending
- 2010-10-04 WO PCT/US2010/051358 patent/WO2011041796A1/en not_active Ceased
- 2010-10-04 CA CA2775093A patent/CA2775093A1/en not_active Abandoned
- 2010-10-04 BR BR112012007516A patent/BR112012007516A2/pt not_active IP Right Cessation
- 2010-10-04 AR ARP100103605A patent/AR078502A1/es unknown
- 2010-10-04 US US12/897,489 patent/US20110252505A1/en not_active Abandoned
- 2010-10-04 IN IN2408DEN2012 patent/IN2012DN02408A/en unknown
- 2010-10-04 EP EP10787210A patent/EP2483404A1/en not_active Withdrawn
-
2013
- 2013-04-22 US US13/867,594 patent/US20130326730A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AR078502A1 (es) | 2011-11-09 |
| MX2012003972A (es) | 2012-05-08 |
| EP2483404A1 (en) | 2012-08-08 |
| CN102597229A (zh) | 2012-07-18 |
| BR112012007516A2 (pt) | 2015-09-15 |
| IN2012DN02408A (forum.php) | 2015-08-21 |
| US20130326730A1 (en) | 2013-12-05 |
| US20110252505A1 (en) | 2011-10-13 |
| WO2011041796A1 (en) | 2011-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9677084B2 (en) | Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance | |
| US8987553B2 (en) | Modulation of ACC synthase improves plant yield under low nitrogen conditions | |
| US20100115662A1 (en) | Manipulation of glutamine synthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants | |
| US8252979B2 (en) | Manipulation of ammonium transporters (AMTS) to improve nitrogen use efficiency in higher plants | |
| CN101421295A (zh) | 用于提高作物植物中的氮利用效率的基因 | |
| US20160010101A1 (en) | Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize | |
| US20130326730A1 (en) | Down-regulation of acc synthase for improved plant performance | |
| US8269066B2 (en) | Nitrate reductases from red algae, compositions and methods of use thereof | |
| WO2014031675A2 (en) | Down-regulation of bzip transcription factor genes for improved plant performance | |
| US9701975B2 (en) | Down-regulation of auxin responsive genes for improved plant performance | |
| US20090158459A1 (en) | Novel transcription factor for increasing kernel mass and yield in plants (1403) | |
| US20160017360A1 (en) | Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield | |
| US20090151022A1 (en) | Novel transcription factor for increasing kernel mass and yield in plants (831) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20161005 |