CA2733990C - Biomarker detection process and assay of neurological condition - Google Patents
Biomarker detection process and assay of neurological condition Download PDFInfo
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US8492107B2 (en) | 2004-04-15 | 2013-07-23 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
KR20100105776A (ko) | 2008-01-18 | 2010-09-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 체액 내에서 질병 또는 병태의 시그너쳐의 검출 방법 |
US20110143375A1 (en) * | 2008-08-11 | 2011-06-16 | Banyan Blomakers, Inc. | Biomarker detection process and assay of neurological condition |
US9874573B2 (en) * | 2008-10-02 | 2018-01-23 | Gaia Medical Institute | Health test for a broad spectrum of health problems |
AU2010262952B2 (en) * | 2009-06-19 | 2016-01-07 | Banyan Biomarkers, Inc. | Biomarker assay of neurological condition |
WO2011011334A2 (en) * | 2009-07-18 | 2011-01-27 | Banyan Biomarkers, Inc. | SYNERGISTIC BIOMARKER ASSAY OF NEUROLGICAL CONDITION USING S-100β |
US20130022982A1 (en) | 2009-09-14 | 2013-01-24 | Kevin Ka-Wang Wang | Micro-rna, autoantibody and protein markers for diagnosis of neuronal injury |
US9682132B2 (en) | 2010-01-26 | 2017-06-20 | Banyan Biomarkers, Inc | Compositions and methods relating to argininosucccinate synthetase |
AU2011235892B2 (en) * | 2010-04-01 | 2016-07-07 | Banyan Biomarkers, Inc. | Markers and assays for detection of neurotoxicity |
WO2011160096A2 (en) * | 2010-06-17 | 2011-12-22 | Banyan Biomarkers, Inc. | Glial fibrillary acidic protein, autoantigens and autoantibodies thereto as biomarkers of neural injury or neurological disorder or condition |
CA2806293A1 (en) | 2010-07-23 | 2012-01-26 | President And Fellows Of Harvard College | Methods of detecting autoimmune or immune-related diseases or conditions |
US20130203624A1 (en) | 2010-07-23 | 2013-08-08 | President And Fellows Of Harvard College | Methods of Detecting Prenatal or Pregnancy-Related Diseases or Conditions |
CA2806291C (en) | 2010-07-23 | 2023-08-29 | President And Fellows Of Harvard College | Methods for detecting signatures of disease or conditions in bodily fluids |
CA2806310A1 (en) | 2010-07-23 | 2012-01-26 | President And Fellows Of Harvard College | Methods of detecting diseases or conditions using phagocytic cells |
EP2628013B1 (en) * | 2010-10-14 | 2019-06-12 | The Johns Hopkins University | Biomarkers of brain injury |
WO2012142301A2 (en) | 2011-04-12 | 2012-10-18 | Quanterix Corporation | Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury |
EP2707389B1 (en) | 2011-05-12 | 2019-10-30 | The Johns Hopkins University | Assay reagents for a neurogranin diagnostic kit |
WO2013090285A1 (en) * | 2011-12-14 | 2013-06-20 | University Of Rochester | Method of diagnosing mild traumatic brain injury |
JP6392202B2 (ja) | 2012-03-13 | 2018-09-19 | ザ・ジョンズ・ホプキンス・ユニバーシティ | 脳損傷または神経変性のバイオマーカーとしてのシトルリン化脳および神経タンパク質 |
MX2014015434A (es) | 2012-06-15 | 2015-07-14 | Harry Stylli | Metodos para detectar enfermedades o condiciones utilizando celulas enfermas en circulacion. |
JP2015522260A (ja) | 2012-06-15 | 2015-08-06 | ハリー スティリ, | 疾患または状態を検出する方法 |
EP2965086A4 (en) | 2013-03-09 | 2017-02-08 | Harry Stylli | Methods of detecting prostate cancer |
WO2014164366A1 (en) | 2013-03-09 | 2014-10-09 | Harry Stylli | Methods of detecting cancer |
WO2014144133A1 (en) * | 2013-03-15 | 2014-09-18 | The Trustees Of The Princeton University | Analyte detection enhancement by targeted immobilization, surface amplification, and pixelated reading and analysis |
US20150141528A1 (en) * | 2013-05-31 | 2015-05-21 | Banyan Biomarkers, Inc. | Neural specific s100b for biomarker assays and devices for detection of a neurological condition |
EP3022322A4 (en) | 2013-07-17 | 2017-05-17 | The Johns Hopkins University | A multi-protein biomarker assay for brain injury detection and outcome |
EP3663764B1 (en) * | 2014-04-08 | 2023-11-01 | University of Florida Research Foundation, Inc. | Protein biomarkers for acute, subacute and chronic traumatic injuries of the central nervous system |
EP3693742B1 (en) | 2014-09-11 | 2022-04-06 | Harry Stylli | Methods of detecting prostate cancer |
EP3702786A1 (en) * | 2014-10-06 | 2020-09-02 | Université de Genève | Markers and their use in brain injury |
US20190010504A1 (en) * | 2015-12-11 | 2019-01-10 | Arizona Board Of Regents On Behalf Of Arizona State University | Human alzheimer's disease and traumatic brain injury associated tau variants as biomarkers and methods of use thereof |
BR112019006710A2 (pt) * | 2016-10-03 | 2019-06-25 | Abbott Lab | métodos aprimorados para avaliação do estado da uch-l1 em amostras de pacientes |
CN110366558A (zh) | 2016-10-28 | 2019-10-22 | 班扬生物标记公司 | 针对泛素c末端水解酶l1(uch-l1)和胶质纤维酸性蛋白(gfap)的抗体及相关方法 |
CA3052636A1 (en) * | 2017-02-07 | 2018-08-16 | Bioregency, Inc. | S100.beta. and isoforms for detection of neurological conditions |
US10345302B2 (en) * | 2017-02-19 | 2019-07-09 | Sheng-He Huang | Circulating astrocytes and MFSD2A as biomarkers |
JP7346300B2 (ja) * | 2017-03-23 | 2023-09-19 | アボット・ラボラトリーズ | 早期バイオマーカーであるユビキチンカルボキシ末端ヒドロラーゼl1を使用する、ヒト対象における外傷性脳損傷の程度の診断及び決定の一助となるための方法 |
EP3602069A1 (en) * | 2017-03-23 | 2020-02-05 | Abbott Laboratories | Methods for aiding in the diagnosis and determination of the extent of traumatic brain injury in a human subject using the early biomarker ubiquitin carboxy-terminal hydrolase l1 |
AU2018250688B2 (en) | 2017-04-15 | 2024-07-04 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury in a human subject using early biomarkers |
AU2018256845B2 (en) | 2017-04-28 | 2024-03-14 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury using early biomarkers on at least two samples from the same human subject |
WO2018218169A1 (en) * | 2017-05-25 | 2018-11-29 | Abbott Laboratories | Methods for aiding in the determination of whether to perform imaging on a human subject who has sustained or may have sustained an injury to the head using early biomarkers |
BR112019025387A2 (pt) * | 2017-05-30 | 2020-07-07 | Abbott Laboratories | métodos para auxiliar no diagnóstico e avaliação de uma lesão traumática cerebral branda em um indivíduo humano com o uso de troponina cardíaca i e biomarcadores precoces |
WO2019010131A1 (en) | 2017-07-03 | 2019-01-10 | Abbott Laboratories | IMPROVED METHODS FOR MEASURING CARBOXY TERMINATION HYDROLASE LEVELS OF UBIQUITIN L1 IN BLOOD |
EP3655545B1 (en) | 2017-07-18 | 2023-10-18 | The Research Foundation for The State University of New York | Biomarkers for intracranial aneurysm |
CA3067055A1 (en) * | 2017-12-09 | 2019-06-13 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a traumatic brain injury in a human subject using a combination of gfap and uch-l1 |
BR112019028254A2 (pt) | 2017-12-09 | 2020-07-14 | Abbott Laboratories | métodos para ajudar no diagnóstico e avaliação de um paciente que sofreu uma lesão ortopédica e que sofreu ou pode ter sofrido uma lesão na cabeça, tal como uma lesão cerebral traumática (lct) leve, usando a proteína ácida fibrilar glial (gfap) e/ou a hidrolase carbóxi-terminal da ubiquitina l1 (uch-l1) |
WO2019133717A1 (en) | 2017-12-29 | 2019-07-04 | Abbott Laboratories | Novel biomarkers and methods for diagnosing and evaluating traumatic brain injury |
US20220229073A1 (en) | 2018-04-10 | 2022-07-21 | Quanterix Corporation | Quantification of neurofilament light chain in physiological samples |
KR102179032B1 (ko) * | 2019-09-20 | 2020-11-18 | 한림대학교 산학협력단 | 급성허혈성뇌손상 및 급성외상성뇌손상을 구별하기 위한 바이오마커 및 이의 이용 |
JP2023551542A (ja) | 2020-11-30 | 2023-12-08 | エニグマ バイオインテリジェンス,インコーポレイテッド | アルツハイマー病の非侵襲的評価 |
CN116879564A (zh) * | 2023-09-06 | 2023-10-13 | 暨南大学附属第一医院(广州华侨医院) | 基于蛋白质组学及磷酸化蛋白质修饰组学的脊髓损伤生物标志物及其应用 |
Family Cites Families (120)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4376110A (en) | 1980-08-04 | 1983-03-08 | Hybritech, Incorporated | Immunometric assays using monoclonal antibodies |
US4366241A (en) | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4517288A (en) | 1981-01-23 | 1985-05-14 | American Hospital Supply Corp. | Solid phase system for ligand assay |
US4702909A (en) | 1982-05-05 | 1987-10-27 | Louisiana State University A & M | Non-A, non-B hepatitis antigen, antigen compositions, vaccine and diagnostic reagent |
CA1291031C (en) | 1985-12-23 | 1991-10-22 | Nikolaas C.J. De Jaeger | Method for the detection of specific binding agents and their correspondingbindable substances |
US5231000A (en) | 1987-10-08 | 1993-07-27 | The Mclean Hospital | Antibodies to A4 amyloid peptide |
US5118606A (en) | 1988-09-02 | 1992-06-02 | The Regents Of The University Of California | Methods for detecting cellular pathology by assaying spectrin and spectrin breakdown products |
US5234814A (en) | 1989-06-01 | 1993-08-10 | Du Pont Merck Pharmaceutical Company | Diagnostic assay for alzheimer's disease |
US5118937A (en) | 1989-08-22 | 1992-06-02 | Finnigan Mat Gmbh | Process and device for the laser desorption of an analyte molecular ions, especially of biomolecules |
US5045694A (en) | 1989-09-27 | 1991-09-03 | The Rockefeller University | Instrument and method for the laser desorption of ions in mass spectrometry |
EP0732399A3 (en) | 1990-03-05 | 1997-03-12 | Cephalon Inc | Chymotrypsin-like proteases and their inhibitors |
US5586226A (en) | 1990-05-16 | 1996-12-17 | Canon Kabushiki Kaisha | Control method and device for a unicolor printer |
US5707796A (en) | 1990-06-11 | 1998-01-13 | Nexstar Pharmaceuticals, Inc. | Method for selecting nucleic acids on the basis of structure |
US6011020A (en) | 1990-06-11 | 2000-01-04 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand complexes |
US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
ATE318832T1 (de) | 1990-06-11 | 2006-03-15 | Gilead Sciences Inc | Verfahren zur vervendung von nukleinsäureliganden |
US5683867A (en) | 1990-06-11 | 1997-11-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: blended SELEX |
US5637459A (en) | 1990-06-11 | 1997-06-10 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: chimeric selex |
US5496938A (en) | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
US5660985A (en) | 1990-06-11 | 1997-08-26 | Nexstar Pharmaceuticals, Inc. | High affinity nucleic acid ligands containing modified nucleotides |
US5252463A (en) | 1990-06-22 | 1993-10-12 | The Du Pont Merck Pharmaceutical Company | Clipsin, a chymotrypsin-like protease and method of using same |
EP0600951A4 (en) | 1991-08-01 | 1996-10-30 | Paul H Voorheis | Diagnostic method for alzheimer's disease. |
US5792664A (en) | 1992-05-29 | 1998-08-11 | The Rockefeller University | Methods for producing and analyzing biopolymer ladders |
DE69334274D1 (de) | 1992-05-29 | 2009-05-14 | Univ Rockefeller | Verfahren zur Bestimmung der Folge von Peptiden unter Verwendung eines Massenspektrometers |
CA2163426C (en) | 1993-05-28 | 2005-11-01 | T. William Hutchens | Method and apparatus for desorption and ionization of analytes |
DE4337654C2 (de) | 1993-11-04 | 1996-08-08 | Biotest Pharma Gmbh | Verwendung boviner Kolostralmilch von nicht mit Viren hyperimmunisierten Tieren als Leberschutzpräparat |
US5536639A (en) * | 1994-03-25 | 1996-07-16 | Cephalon, Inc. | Methods for detecting calpain activation by detection of calpain activated spectrin breakdown products |
ATE268816T1 (de) * | 1994-07-15 | 2004-06-15 | Cephalon Inc | In baculovirus exprimiertes aktives calpain |
US5614649A (en) | 1994-11-14 | 1997-03-25 | Cephalon, Inc. | Multicatalytic protease inhibitors |
US5777194A (en) * | 1995-04-26 | 1998-07-07 | Cephalon, Inc. | Gene-targeted mice with humanized Aβ sequence and Swedish FAD mutation |
DE69635979T2 (de) | 1996-02-02 | 2006-11-16 | Biotrin Intellectual Properties Ltd., Mount Merrion | Verfahren zur bestimmung des hepatischen zustands eines lebertransplantationsempfängers. |
US6048703A (en) * | 1996-11-15 | 2000-04-11 | Cephalon, Inc. | Methods for detecting cell apoptosis |
NZ516848A (en) | 1997-06-20 | 2004-03-26 | Ciphergen Biosystems Inc | Retentate chromatography apparatus with applications in biology and medicine |
EP1037626A1 (en) | 1997-12-16 | 2000-09-27 | Cephalon, Inc. | Multicatalytic protease inhibitors for use as anti-tumor agents |
CA2323638A1 (en) | 1998-04-03 | 1999-10-14 | Phylos, Inc. | Addressable protein arrays |
US6406921B1 (en) | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
ES2229817T3 (es) | 1998-09-08 | 2005-04-16 | Innogenetics N.V. | Uso de la tau como un marcador para la deteccion temprana del daño del sistema nervioso central (cns). |
AU4025300A (en) | 1999-03-24 | 2000-10-09 | Packard Bioscience Company | Continuous porous matrix arrays |
US6218005B1 (en) * | 1999-04-01 | 2001-04-17 | 3M Innovative Properties Company | Tapes for heat sealing substrates |
US20020002270A1 (en) | 1999-06-16 | 2002-01-03 | Raymond P. Zinkowski | Purified antigen for alzheimer's disease, and methods of obtaining and using same |
US6589746B1 (en) | 1999-10-21 | 2003-07-08 | University Of Cincinnati | Method of detecting axonally-derived protein tau in patients with traumatic CNS injury |
US6664442B2 (en) * | 2000-03-30 | 2003-12-16 | Elan Pharmaceuticals, Inc. | Selecting compounds to reduce inflammation associated with Alzheimer's disease |
US20050063942A1 (en) | 2001-02-02 | 2005-03-24 | Clark Mike A. | Methods for predicting sensitivity of tumors to arginine deprivation |
US6949377B2 (en) | 2001-03-05 | 2005-09-27 | Ho Winston Z | Chemiluminescence-based microfluidic biochip |
US7427490B2 (en) | 2001-08-20 | 2008-09-23 | Biosite Incorporated | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
US20040253637A1 (en) | 2001-04-13 | 2004-12-16 | Biosite Incorporated | Markers for differential diagnosis and methods of use thereof |
US20040203083A1 (en) | 2001-04-13 | 2004-10-14 | Biosite, Inc. | Use of thrombus precursor protein and monocyte chemoattractant protein as diagnostic and prognostic indicators in vascular diseases |
US7713705B2 (en) | 2002-12-24 | 2010-05-11 | Biosite, Inc. | Markers for differential diagnosis and methods of use thereof |
US20030199000A1 (en) | 2001-08-20 | 2003-10-23 | Valkirs Gunars E. | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
US20020172676A1 (en) | 2001-05-16 | 2002-11-21 | George Jackowski | Method of treatment of alzheimer's disease and device therefor |
US7144708B2 (en) | 2001-06-25 | 2006-12-05 | The Cleveland Clinic Foundation | Markers of blood barrier disruption and methods of using same |
JP2003070498A (ja) | 2001-07-19 | 2003-03-11 | Pharma Design Inc | ユビキチンカルボキシルターミナルヒドロラーゼl1遺伝子異常の検出方法 |
US20030040660A1 (en) | 2001-08-27 | 2003-02-27 | George Jackowski | Method for diagnosing and distinguishing traumatic brain injury and diagnostic devices for use therein |
AU2002329570A1 (en) | 2001-10-12 | 2003-01-30 | Pfizer Products Inc. | Method of monitoring neuroprotective treatment |
WO2003062824A1 (en) | 2002-01-23 | 2003-07-31 | Boditech Inc. | Lateral flow quantitative assay method and strip and laser-induced fluoerescence detection device therefor |
AU2003214981A1 (en) | 2002-01-31 | 2003-09-02 | Gene Logic, Inc. | Molecular hepatotoxicology modeling |
WO2003085083A2 (en) | 2002-04-01 | 2003-10-16 | Phase-1 Molecular Toxicology, Inc. | Liver necrosis predictive genes |
AU2003243563A1 (en) | 2002-06-12 | 2004-09-28 | The Cleveland Clinic Foundation | Markers of blood barrier permeability and methods of using same |
WO2004025298A1 (en) | 2002-09-11 | 2004-03-25 | University Of Florida | Analyzing nerve cell damage |
EP1587955A4 (en) | 2002-12-24 | 2007-03-14 | Biosite Inc | DIFFERENTIAL DIAGNOSTIC MARKERS AND METHODS OF USE |
WO2005004794A2 (en) | 2003-06-09 | 2005-01-20 | Alnylam Pharmaceuticals Inc. | Method of treating neurodegenerative disease |
GB0322063D0 (en) * | 2003-09-20 | 2003-10-22 | Univ Geneve | Diagnostic method for brain damage-related disorders |
CA2532130C (en) | 2003-09-20 | 2016-07-19 | Electrophoretics Limited | Diagnostic method for brain damage-related disorders |
EP1519194A1 (en) | 2003-09-24 | 2005-03-30 | Roche Diagnostics GmbH | Use of gfap for identification of intracerebral hemorrhage |
US20050130226A1 (en) | 2003-09-26 | 2005-06-16 | The University Of Cincinnati | Fully integrated protein lab-on-a-chip with smart microfluidics for spot array generation |
EP1694816B1 (en) | 2003-11-07 | 2013-08-28 | Ciphergen Biosystems, Inc. | Biomarkers for alzheimer's disease |
US20060094064A1 (en) | 2003-11-19 | 2006-05-04 | Sandip Ray | Methods and compositions for diagnosis, stratification, and monitoring of alzheimer's disease and other neurological disorders in body fluids |
US20050112585A1 (en) | 2003-11-21 | 2005-05-26 | Dominic Zichi | Method for adjusting the quantification range of individual analytes in a multiplexed assay |
TWI281473B (en) | 2003-12-19 | 2007-05-21 | Ind Tech Res Inst | Biomarkers for liver diseases and method for using same |
AU2004312893B2 (en) | 2003-12-31 | 2010-06-17 | President And Fellows Of Harvard College | Assay device and method |
CA2578670C (en) | 2004-04-15 | 2017-03-07 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
US20140303041A1 (en) | 2004-04-15 | 2014-10-09 | University Of Florida Research Foundation Inc. | In vitro diagnostic devices for nervous system injury and other neural disorders |
US8492107B2 (en) | 2004-04-15 | 2013-07-23 | University Of Florida Research Foundation, Inc. | Neural proteins as biomarkers for nervous system injury and other neural disorders |
ES2367311T3 (es) | 2004-04-15 | 2011-11-02 | University Of Florida Research Foundation, Inc. | Productos de degradación proteolítica de map-2 como biomarcadores de diagnóstico para las lesiones neurales. |
US20060292558A1 (en) | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
JP4546534B2 (ja) | 2004-10-15 | 2010-09-15 | シーメンス アクチエンゲゼルシヤフト | 使い捨てカートリッジ内のdnaまたはタンパク質の総合的かつ自動的な分析装置、このようなカートリッジの製造方法、およびこのようなカートリッジを使用したdnaまたはタンパク質分析のための操作方法 |
US8048638B2 (en) | 2005-04-01 | 2011-11-01 | University Of Florida Research Foundation, Inc. | Biomarkers of liver injury |
JP5202296B2 (ja) | 2005-04-01 | 2013-06-05 | ユニバーシティ オブ フロリダ リサーチ ファウンデーション,インコーポレイティド | 肝臓傷害のバイオマーカー |
GB2428240A (en) | 2005-07-14 | 2007-01-24 | Univ Gen Ve | Diagnostic method for brain damage-related disorders |
WO2007033385A2 (en) | 2005-09-13 | 2007-03-22 | Fluidigm Corporation | Microfluidic assay devices and methods |
EP1949103A2 (en) | 2005-10-20 | 2008-07-30 | Biosite Incorporated | Diagnostic markers of stroke and cerebral injury and methods of use thereof |
AU2007204589B2 (en) | 2006-01-12 | 2012-11-01 | Mycrolab Diagnostics Pty Ltd | New instrumentation systems and methods |
EP1992688B1 (en) | 2006-02-15 | 2016-06-22 | Mie University | Method of constructing recombinant proteoliposome for diagnostic use |
US20070255113A1 (en) | 2006-05-01 | 2007-11-01 | Grimes F R | Methods and apparatus for identifying disease status using biomarkers |
WO2007136617A2 (en) | 2006-05-18 | 2007-11-29 | Walter Reed Army Institute Of Research (Wrair) | Endothelial-monocyte activating polypeptide ii, a biomarker for use in diagnosis of brain injury |
EP2024395A4 (en) | 2006-05-26 | 2009-07-01 | Biosite Inc | USE OF NATRIURETIC PEPTIDES AS DIAGNOSTIC AND PROGNOSTIC INDICATORS IN VASCULAR DISEASES |
WO2008008819A2 (en) | 2006-07-11 | 2008-01-17 | University Of Florida Research Foundation, Inc. | Diagnosis and treatment of neurological inflammation |
US20100159486A1 (en) | 2006-11-01 | 2010-06-24 | George Mason Intellectual Properties, Inc. | Biomarkers for neurological conditions |
WO2008095136A2 (en) | 2007-01-31 | 2008-08-07 | Henkin Robert I | Methods for detection of biological substances |
US20120196307A1 (en) | 2007-02-06 | 2012-08-02 | Banyan Biomarkers, Inc. | Synaptotagmin and collapsin response mediator protein as biomarkers for traumatic brain injury |
WO2008097618A1 (en) | 2007-02-06 | 2008-08-14 | University Of Florida Research Foundation, Inc. | Synaptotagmin and collapsin response mediator protein as biomarkers for traumatic brain injury |
CN101983337A (zh) | 2008-02-04 | 2011-03-02 | 班扬生物标记公司 | 用于诊断或治疗脑损伤的方法 |
WO2009129476A2 (en) | 2008-04-17 | 2009-10-22 | Banyan Biomarkers, Inc. | An antibody bound synthetic vesicle containing active agent molecules |
EP2143735A1 (en) | 2008-07-10 | 2010-01-13 | Institut Pasteur | Variable domains of camelid heavy-chain antibodies directed against glial fibrillary acidic proteins |
US20110143375A1 (en) * | 2008-08-11 | 2011-06-16 | Banyan Blomakers, Inc. | Biomarker detection process and assay of neurological condition |
US20140342381A1 (en) | 2008-08-11 | 2014-11-20 | Banyan Biomarkers, Inc. | Devices and methods for biomarker detection process and assay of neurological condition |
EP2340030B1 (en) | 2008-09-19 | 2016-08-17 | Henry Ford Health System | Methods, systems, and compositions for calpain inhibition |
WO2010059242A2 (en) | 2008-11-21 | 2010-05-27 | The Johns Hopkins University | Neurodegenerative disease diagnostic compositions and methods of use |
US20170315136A9 (en) | 2009-06-19 | 2017-11-02 | Banyan Biomarkers, Inc. | Biomarker assay of neurological condition |
AU2010262952B2 (en) | 2009-06-19 | 2016-01-07 | Banyan Biomarkers, Inc. | Biomarker assay of neurological condition |
WO2011011334A2 (en) | 2009-07-18 | 2011-01-27 | Banyan Biomarkers, Inc. | SYNERGISTIC BIOMARKER ASSAY OF NEUROLGICAL CONDITION USING S-100β |
US20130022982A1 (en) | 2009-09-14 | 2013-01-24 | Kevin Ka-Wang Wang | Micro-rna, autoantibody and protein markers for diagnosis of neuronal injury |
US9682132B2 (en) | 2010-01-26 | 2017-06-20 | Banyan Biomarkers, Inc | Compositions and methods relating to argininosucccinate synthetase |
AU2011235892B2 (en) | 2010-04-01 | 2016-07-07 | Banyan Biomarkers, Inc. | Markers and assays for detection of neurotoxicity |
WO2011160096A2 (en) | 2010-06-17 | 2011-12-22 | Banyan Biomarkers, Inc. | Glial fibrillary acidic protein, autoantigens and autoantibodies thereto as biomarkers of neural injury or neurological disorder or condition |
EP2628013B1 (en) | 2010-10-14 | 2019-06-12 | The Johns Hopkins University | Biomarkers of brain injury |
JP6061935B2 (ja) | 2011-09-14 | 2017-01-18 | ザ ヘンリー エム. ジャクソン ファウンデーション フォー ザ アドバンスメント オブ ミリタリー メディシン,インコーポレーテッド | 心的外傷後ストレス障害(ptsd)のための診断用バイオマーカーを検出及び監視するため、並びに同障害の自殺型と非自殺型とを識別するためのプロセス及びキット |
US20140018299A1 (en) | 2012-07-10 | 2014-01-16 | Banyan Biomarkers, Inc. | Method and device to detect, monitor and promote neural regeneration and improvement of cognitive function in a subject suffering from neural injury |
US20140024053A1 (en) | 2012-07-20 | 2014-01-23 | Banyan Biomarkers, Inc. | Methods, kits and devices for detecting bii-spectrin, and breakdown products thereof, as biomarkers for the diagnosis of neural injury |
US20140275294A1 (en) | 2013-03-15 | 2014-09-18 | Banyan Biomarkers, Inc. | Devices and methods for biomarker detection process and assay of liver injury |
US20150141528A1 (en) | 2013-05-31 | 2015-05-21 | Banyan Biomarkers, Inc. | Neural specific s100b for biomarker assays and devices for detection of a neurological condition |
EP3129780A4 (en) | 2014-04-07 | 2017-12-13 | Iron Horse Diagnostics Inc. | Traumatic brain injury and neurodegenerative biomarkers, methods, and systems |
EP3663764B1 (en) | 2014-04-08 | 2023-11-01 | University of Florida Research Foundation, Inc. | Protein biomarkers for acute, subacute and chronic traumatic injuries of the central nervous system |
CN110366558A (zh) | 2016-10-28 | 2019-10-22 | 班扬生物标记公司 | 针对泛素c末端水解酶l1(uch-l1)和胶质纤维酸性蛋白(gfap)的抗体及相关方法 |
AU2018250688B2 (en) * | 2017-04-15 | 2024-07-04 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury in a human subject using early biomarkers |
AU2018256845B2 (en) * | 2017-04-28 | 2024-03-14 | Abbott Laboratories | Methods for aiding in the hyperacute diagnosis and determination of traumatic brain injury using early biomarkers on at least two samples from the same human subject |
BR112019025387A2 (pt) * | 2017-05-30 | 2020-07-07 | Abbott Laboratories | métodos para auxiliar no diagnóstico e avaliação de uma lesão traumática cerebral branda em um indivíduo humano com o uso de troponina cardíaca i e biomarcadores precoces |
CA3067055A1 (en) * | 2017-12-09 | 2019-06-13 | Abbott Laboratories | Methods for aiding in diagnosing and evaluating a traumatic brain injury in a human subject using a combination of gfap and uch-l1 |
BR112019028254A2 (pt) * | 2017-12-09 | 2020-07-14 | Abbott Laboratories | métodos para ajudar no diagnóstico e avaliação de um paciente que sofreu uma lesão ortopédica e que sofreu ou pode ter sofrido uma lesão na cabeça, tal como uma lesão cerebral traumática (lct) leve, usando a proteína ácida fibrilar glial (gfap) e/ou a hidrolase carbóxi-terminal da ubiquitina l1 (uch-l1) |
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NO2324360T3 (US07291463-20071106-C00058.png) | 2018-06-30 |
AU2009282117B2 (en) | 2016-05-12 |
US20180031577A1 (en) | 2018-02-01 |
JP2012500388A (ja) | 2012-01-05 |
JP2014199262A (ja) | 2014-10-23 |
EP4235181A2 (en) | 2023-08-30 |
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