CA2733653C - Detection of target variants using a fluorescent label and a soluble quencher - Google Patents

Detection of target variants using a fluorescent label and a soluble quencher Download PDF

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Publication number
CA2733653C
CA2733653C CA2733653A CA2733653A CA2733653C CA 2733653 C CA2733653 C CA 2733653C CA 2733653 A CA2733653 A CA 2733653A CA 2733653 A CA2733653 A CA 2733653A CA 2733653 C CA2733653 C CA 2733653C
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CA
Canada
Prior art keywords
nucleic acid
label
labeled oligonucleotide
light emission
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2733653A
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English (en)
French (fr)
Other versions
CA2733653A1 (en
Inventor
Stephen Gordon Will
Amar P. Gupta
Laura Geyer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2733653A1 publication Critical patent/CA2733653A1/en
Application granted granted Critical
Publication of CA2733653C publication Critical patent/CA2733653C/en
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA2733653A 2008-09-18 2009-09-16 Detection of target variants using a fluorescent label and a soluble quencher Active CA2733653C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9818608P 2008-09-18 2008-09-18
US61/098,186 2008-09-18
PCT/EP2009/006688 WO2010031539A1 (en) 2008-09-18 2009-09-16 Detection of target variants using a fluorescent labeland and a soluble quencher

Publications (2)

Publication Number Publication Date
CA2733653A1 CA2733653A1 (en) 2010-03-25
CA2733653C true CA2733653C (en) 2016-04-26

Family

ID=41256038

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2733653A Active CA2733653C (en) 2008-09-18 2009-09-16 Detection of target variants using a fluorescent label and a soluble quencher

Country Status (6)

Country Link
US (1) US8658366B2 (enExample)
EP (1) EP2347012B1 (enExample)
JP (1) JP5774990B2 (enExample)
CA (1) CA2733653C (enExample)
ES (1) ES2629460T3 (enExample)
WO (1) WO2010031539A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2971169A1 (en) * 2014-12-30 2016-07-07 Telomere Diagnostics, Inc. Multiplex quantitative pcr
CN114250274A (zh) 2015-04-24 2022-03-29 阿提拉生物系统公司 利用有限核苷酸组成的引物扩增

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9918237D0 (en) 1999-08-04 1999-10-06 Secr Defence Detection system
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
GB0223563D0 (en) 2002-10-10 2002-11-20 Secr Defence Detection system
US8067208B2 (en) * 2005-06-30 2011-11-29 Roche Molecular Systems, Inc. Probes and methods for hepatitis C virus typing using multidimensional probe analysis
US7785786B2 (en) 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
GB0603190D0 (en) 2006-02-16 2006-03-29 Enigma Diagnostics Ltd Detection system

Also Published As

Publication number Publication date
ES2629460T3 (es) 2017-08-09
EP2347012A1 (en) 2011-07-27
US20100124744A1 (en) 2010-05-20
CA2733653A1 (en) 2010-03-25
JP2012502638A (ja) 2012-02-02
JP5774990B2 (ja) 2015-09-09
WO2010031539A1 (en) 2010-03-25
EP2347012B1 (en) 2017-04-19
US8658366B2 (en) 2014-02-25

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Legal Events

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