CA2707446A1 - Industrial production of organic compounds using recombinant organisms expressing methyl halide transferase - Google Patents
Industrial production of organic compounds using recombinant organisms expressing methyl halide transferase Download PDFInfo
- Publication number
- CA2707446A1 CA2707446A1 CA2707446A CA2707446A CA2707446A1 CA 2707446 A1 CA2707446 A1 CA 2707446A1 CA 2707446 A CA2707446 A CA 2707446A CA 2707446 A CA2707446 A CA 2707446A CA 2707446 A1 CA2707446 A1 CA 2707446A1
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- CA
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- Prior art keywords
- organism
- sam
- halide
- mht
- methyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 238000000034 method Methods 0.000 claims abstract description 99
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (9)
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|---|---|---|---|
| US99167807P | 2007-11-30 | 2007-11-30 | |
| US60/991,678 | 2007-11-30 | ||
| US3836808P | 2008-03-20 | 2008-03-20 | |
| US61/038,368 | 2008-03-20 | ||
| US4146708P | 2008-04-01 | 2008-04-01 | |
| US61/041,467 | 2008-04-01 | ||
| US9822108P | 2008-09-18 | 2008-09-18 | |
| US61/098,221 | 2008-09-18 | ||
| PCT/US2008/085019 WO2009073560A1 (en) | 2007-11-30 | 2008-11-26 | Industrial production of organic compounds using recombinant organisms expressing methyl halide transferase |
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| CA2707446A1 true CA2707446A1 (en) | 2009-06-11 |
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| CA2707446A Abandoned CA2707446A1 (en) | 2007-11-30 | 2008-11-26 | Industrial production of organic compounds using recombinant organisms expressing methyl halide transferase |
| CA2706894A Abandoned CA2706894A1 (en) | 2007-11-30 | 2008-11-26 | Biological systems for production of commercially valuable compounds |
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| CA2706894A Abandoned CA2706894A1 (en) | 2007-11-30 | 2008-11-26 | Biological systems for production of commercially valuable compounds |
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| EP (2) | EP2238239A4 (cg-RX-API-DMAC7.html) |
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| JP2011505149A (ja) | 2007-11-30 | 2011-02-24 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | ハロゲン化メチルトランスフェラーゼを発現する組換え型生物を用いた、有機化合物の工業的生産方法 |
| WO2011011627A1 (en) * | 2009-07-22 | 2011-01-27 | The Regents Of The University Of California | Cell-based systems for production of methyl formate |
| WO2011088364A2 (en) * | 2010-01-15 | 2011-07-21 | Massachuseits Institute Of Technology | Bioprocess and microbe engineering for total carbon utilization in biofuelproduction |
| EP2540826B1 (en) * | 2010-02-25 | 2015-12-09 | Toyama Prefecture | Method for producing glucuronic acid conjugate using saccharomyces cerevisiae |
| CN102766586B (zh) * | 2011-05-12 | 2013-05-15 | 江南大学 | 中国酱香型白酒生产中高产乙醇低产杂醇油的酵母cgmcc 4747的筛选与应用 |
| AT511856A1 (de) * | 2011-09-01 | 2013-03-15 | Univ Graz Tech | Mittel und verfahren zur alkylierung |
| EP2871234A1 (en) * | 2013-11-06 | 2015-05-13 | Leibniz-Institut für Pflanzenbiochemie (IPB) | S-Adenosylmethionine (SAM) synthase variants for the synthesis of artificial cofactors |
| WO2016181216A1 (en) * | 2015-05-08 | 2016-11-17 | Universidad Adolfo Ibanez | Pgpr compositions and methods for improved cultivation of tomato and potato species |
| WO2016191141A1 (en) * | 2015-05-26 | 2016-12-01 | Sabic Global Technologies, B.V. | Zeolite catalyst for alkyl halide to olefin conversion |
| CN106994134B (zh) * | 2016-01-25 | 2020-08-25 | 深圳华大生命科学研究院 | 肠道益生菌在预防和/或治疗糖尿病及其相关疾病中的应用 |
| PL3432916T3 (pl) | 2016-09-13 | 2020-04-30 | Allergan, Inc. | Niebiałkowo stabilzowane kompozycje toksyny clostridium |
| CN109306333B (zh) * | 2018-10-25 | 2020-06-12 | 北京润世能源技术有限公司 | 一株微生物采油菌w-y8及其应用 |
| CN109536426B (zh) * | 2019-01-25 | 2021-11-16 | 黑龙江大学 | 一株嗜冷菌及其应用 |
| JP2024519839A (ja) | 2021-05-17 | 2024-05-21 | アクアカルチャード フーズ，インコーポレイテッド | 細菌と真菌の共培養物を含む食品生成物 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5940518B2 (ja) * | 1981-10-14 | 1984-10-01 | 工業技術院長 | セルロ−ス含有廃棄物の嫌気性消化方法 |
| US4794080A (en) * | 1984-04-16 | 1988-12-27 | Igene Biotechnology, Inc. | Microbial co-culture production of propionic acid |
| US5643758A (en) * | 1987-03-10 | 1997-07-01 | New England Biolabs, Inc. | Production and purification of a protein fused to a binding protein |
| GB8923998D0 (en) * | 1989-10-25 | 1989-12-13 | Nestle Sa | Food additives |
| US7109005B2 (en) * | 1990-01-15 | 2006-09-19 | Danisco Sweeteners Oy | Process for the simultaneous production of xylitol and ethanol |
| DE69422161T3 (de) * | 1994-05-27 | 2004-06-03 | Agrano Ag | Verfahren zur Gewinnung einer Biomasse und Brottreibmittel |
| ES2142919T3 (es) * | 1994-05-27 | 2000-05-01 | Agrano Ag | Procedimiento de preparacion de medios de cultivo utilizables para el cultivo individual de levaduras y de bacterias lacticas o el cocultivo de levaduras y de bacterias lacticas. |
| BR9813071A (pt) * | 1997-10-17 | 2000-08-15 | Nestle Sa | Espécie de bactéria láctica |
| AU771630B2 (en) * | 1998-05-06 | 2004-04-01 | Keijiro Nakamura | Microbial culture liquors containing microorganisms differing in characteristics and living in symbiosis and metabolites thereof, carriers and adsorbents containing the active components of the culture liquors and utilization of the same |
| US6610504B1 (en) * | 2000-04-10 | 2003-08-26 | General Atomics | Methods of determining SAM-dependent methyltransferase activity using a mutant SAH hydrolase |
| AU2001284793A1 (en) * | 2000-08-11 | 2002-02-25 | Cornell Research Foundation Inc. | Production and use of inducible enzymes from trichoderma and bacteria for control of plant pests and for industrial processes |
| US20050027084A1 (en) * | 2001-05-23 | 2005-02-03 | Clarke William D. | Production of vinyl halide from single carbon feedstocks |
| US20030152586A1 (en) * | 2002-01-25 | 2003-08-14 | Biomedis | Dietary supplements comprising growth media |
| US7977049B2 (en) * | 2002-08-09 | 2011-07-12 | President And Fellows Of Harvard College | Methods and compositions for extending the life span and increasing the stress resistance of cells and organisms |
| JP2004261150A (ja) * | 2003-03-04 | 2004-09-24 | Ajinomoto Co Inc | 目的物質の製造法 |
| DE10309856A1 (de) * | 2003-03-06 | 2004-09-23 | Consortium für elektrochemische Industrie GmbH | Verfahren zur fermentativen Herstellung von S-Adenosylmethionin |
| EP1871883A1 (en) * | 2005-03-02 | 2008-01-02 | Metanomics GmbH | Process for the production of fine chemicals |
| KR101359622B1 (ko) * | 2005-04-15 | 2014-02-07 | 유니버시티 오브 써던 캘리포니아 | 메탄의 메탄올, 디메틸 에테르 및 유도된 생성물들로의선택적 산화성 변환 |
| AU2006261356A1 (en) * | 2005-06-17 | 2006-12-28 | Microbia, Inc. | Improved amino acid and metabolite biosynthesis |
| CN101522904A (zh) * | 2006-01-27 | 2009-09-02 | 麻萨诸塞州大学 | 生产生物燃料和相关材料的系统和方法 |
| JP2011505149A (ja) | 2007-11-30 | 2011-02-24 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | ハロゲン化メチルトランスフェラーゼを発現する組換え型生物を用いた、有機化合物の工業的生産方法 |
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| CN101932694A (zh) | 2010-12-29 |
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| WO2009073560A1 (en) | 2009-06-11 |
| BRPI0819649A2 (pt) | 2017-01-10 |
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| US20110151534A1 (en) | 2011-06-23 |
| US9657279B2 (en) | 2017-05-23 |
| US20110165618A1 (en) | 2011-07-07 |
| CN101932699A (zh) | 2010-12-29 |
| WO2009073557A3 (en) | 2009-12-30 |
| AU2008334090A1 (en) | 2009-06-11 |
| EP2238239A2 (en) | 2010-10-13 |
| CA2706894A1 (en) | 2009-06-11 |
| WO2009073557A2 (en) | 2009-06-11 |
| EP2238239A4 (en) | 2011-07-06 |
| EP2222833A4 (en) | 2011-05-18 |
| BRPI0819648A2 (pt) | 2017-05-23 |
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