CA2681145C - Delta-8 desaturases and their use in making polyunsaturated fatty acids - Google Patents
Delta-8 desaturases and their use in making polyunsaturated fatty acids Download PDFInfo
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- CA2681145C CA2681145C CA2681145A CA2681145A CA2681145C CA 2681145 C CA2681145 C CA 2681145C CA 2681145 A CA2681145 A CA 2681145A CA 2681145 A CA2681145 A CA 2681145A CA 2681145 C CA2681145 C CA 2681145C
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- AQWHMKSIVLSRNY-UHFFFAOYSA-N trans-Octadec-5-ensaeure Natural products CCCCCCCCCCCCC=CCCCC(O)=O AQWHMKSIVLSRNY-UHFFFAOYSA-N 0.000 description 1
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- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- RYYVLZVUVIJVGH-UHFFFAOYSA-N trimethylxanthine Natural products CN1C(=O)N(C)C(=O)C2=C1N=CN2C RYYVLZVUVIJVGH-UHFFFAOYSA-N 0.000 description 1
- PIEPQKCYPFFYMG-UHFFFAOYSA-N tris acetate Chemical compound CC(O)=O.OCC(N)(CO)CO PIEPQKCYPFFYMG-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
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- 239000008158 vegetable oil Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0083—Miscellaneous (1.14.99)
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Physics & Mathematics (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
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| US91083107P | 2007-04-10 | 2007-04-10 | |
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| PCT/US2008/059844 WO2008124806A2 (en) | 2007-04-10 | 2008-04-10 | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
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| CA (2) | CA2681145C (OSRAM) |
| DK (1) | DK2132317T3 (OSRAM) |
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| US7794701B2 (en) * | 2007-04-16 | 2010-09-14 | E.I. Du Pont De Nemours And Company | Δ-9 elongases and their use in making polyunsaturated fatty acids |
| CN101970638B (zh) * | 2007-10-03 | 2015-02-11 | 纳幕尔杜邦公司 | 用于高水平生产二十碳五烯酸的优化解脂耶氏酵母菌株 |
| CA2765911C (en) | 2009-06-16 | 2018-12-18 | E.I. Du Pont De Nemours And Company | High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica |
| US20130101723A1 (en) | 2009-11-03 | 2013-04-25 | Daniel Verkoeijen | Vegetable oil comprising a polyunsaturated fatty acid having at least 20 carbon atoms |
| KR101889561B1 (ko) | 2009-11-03 | 2018-08-17 | 디에스엠 아이피 어셋츠 비.브이. | 세포 및 20개 이상의 탄소 원자를 가진 다가불포화 지방산을 포함하는 조성물 |
| WO2011133610A1 (en) | 2010-04-22 | 2011-10-27 | E. I. Du Pont De Nemours And Company | Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass |
| BR112013004356A2 (pt) | 2010-08-26 | 2017-06-27 | Du Pont | polinucleotídeo isolado, polipeptídeo mutante, constructo recombinante, célula transformada, método para produção de um ácido graxo poli-insaturado, óleo microbiano e célula hospedeira microbiana recombinante. |
| JP2013535988A (ja) | 2010-08-26 | 2013-09-19 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用 |
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| US8735137B2 (en) | 2010-12-30 | 2014-05-27 | E I Du Pont De Nemours And Company | Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization |
| US20130040340A1 (en) | 2011-02-07 | 2013-02-14 | E. I. Du Pont De Nemours And Company | Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms |
| BR112015014844A2 (pt) | 2012-12-21 | 2019-01-15 | Du Pont | célula microbiana recombinante e método de produção de um óleo microbiano |
| CA2902002C (en) | 2013-03-14 | 2023-08-01 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| BR112015023709A2 (pt) | 2013-03-15 | 2017-07-18 | Pioneer Hi Bred Int | polipeptídeo phi-4, polinucleotídeo, composição, método para inibir o crescimento, método para controlar uma população, planta, semente, cassete de expressão, método para expressar em uma planta um polinucleotídeo, método para proteger uma planta, proteína de fusão |
| BR112016003225B1 (pt) | 2013-08-16 | 2022-10-25 | Pioneer Hi-Bred International, Inc. | Polipeptídeo pip-47, polipeptídeo pip-47 quimérico, composição, proteína de fusão, método para controlar uma população de inseto-praga, método para inibir o crescimento ou matar um inseto-praga, construto de dna, polinucleotídeo isolado, cassete de expressão, método de obtenção de uma planta transgênica e método para controlar infestação de inseto |
| BR112016005543B1 (pt) | 2013-09-13 | 2022-03-08 | Pioneer Hi-Bred International, Inc | Polipeptídeo pip-72 recombinante, construto de dna, método de obtenção de planta transgênica, célula hospedeira, composição, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto, método para controlar uma infestação de insetos em uma planta transgênica, método para identificar em uma amostra biológica uma sequência de nucleotídeos, método para identificar em uma amostra um polipeptídeo pip-72 |
| WO2015120270A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International, Inc. | Insecticidal proteins and methods for their use |
| US10227608B2 (en) | 2014-02-07 | 2019-03-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| WO2016044092A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi Bred International Inc | Compositions and methods to control insect pests |
| EP3207143B1 (en) | 2014-10-16 | 2023-11-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| EP3244727B1 (en) | 2015-01-15 | 2025-10-22 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| RU2017144238A (ru) | 2015-05-19 | 2019-06-19 | Пайонир Хай-Бред Интернэшнл, Инк. | Инсектицидные белки и способы их применения |
| CN107771181A (zh) | 2015-06-16 | 2018-03-06 | 先锋国际良种公司 | 用以防治昆虫有害生物的组合物和方法 |
| BR122023016256B8 (pt) | 2015-08-06 | 2024-04-30 | Du Pont | Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo |
| WO2017040343A1 (en) | 2015-08-28 | 2017-03-09 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
| CN105137844B (zh) * | 2015-08-31 | 2017-09-01 | 广东电网有限责任公司电力科学研究院 | 锅炉调试过程中风险预控方法和系统 |
| EP3390431A1 (en) | 2015-12-18 | 2018-10-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| US11008585B2 (en) | 2016-05-04 | 2021-05-18 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CA3022858A1 (en) | 2016-06-16 | 2017-12-21 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| EP3478052B1 (en) | 2016-07-01 | 2021-08-25 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
| WO2018084936A1 (en) | 2016-11-01 | 2018-05-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
| CN110087481A (zh) | 2016-12-15 | 2019-08-02 | 帝斯曼知识产权资产管理有限公司 | 包含硅酸盐和含具有至少20个碳原子的多不饱和脂肪酸(lc-pufa)的微生物细胞和/或植物细胞的共混物制剂 |
| EP4659582A2 (en) | 2018-03-14 | 2025-12-10 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
| MX2020009435A (es) | 2018-03-14 | 2020-11-11 | Pioneer Hi Bred Int | Proteinas con actividad insecticida de plantas y metodos para sus usos. |
| EP4182466A2 (en) | 2020-07-14 | 2023-05-24 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
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| US4670285A (en) | 1982-08-06 | 1987-06-02 | The University Of Toronto Innovations Foundation | Infant formula |
| US5188958A (en) | 1986-05-29 | 1993-02-23 | Calgene, Inc. | Transformation and foreign gene expression in brassica species |
| US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US6372965B1 (en) | 1992-11-17 | 2002-04-16 | E.I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants |
| US5968809A (en) | 1997-04-11 | 1999-10-19 | Abbot Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
| US6432684B1 (en) | 1997-04-11 | 2002-08-13 | Abbott Laboratories | Human desaturase gene and uses thereof |
| US6051754A (en) | 1997-04-11 | 2000-04-18 | Abbott Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants |
| PL336077A1 (en) | 1997-04-11 | 2000-06-05 | Calgene Llc | Methods of and compositions for synthesising long-chain polyunsaturated fatty acids in plants |
| US6075183A (en) | 1997-04-11 | 2000-06-13 | Abbott Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants |
| US5972664A (en) | 1997-04-11 | 1999-10-26 | Abbott Laboratories | Methods and compositions for synthesis of long chain poly-unsaturated fatty acids |
| US6403349B1 (en) | 1998-09-02 | 2002-06-11 | Abbott Laboratories | Elongase gene and uses thereof |
| WO2000034439A1 (en) | 1998-12-07 | 2000-06-15 | Washington State University Research Foundation | Desaturases and methods of using them for synthesis of polyunsaturated fatty acids |
| US6825017B1 (en) * | 1998-12-07 | 2004-11-30 | Washington State University Research Foundation | Desaturases and methods of using them for synthesis of polyunsaturated fatty acids |
| SI1309698T1 (sl) | 2000-07-21 | 2007-08-31 | Du Pont | Citokrom p450 encim zdruĺ˝en s sintezo delta-12-epoksi skupin v maĺ äśobnih kislinah rastlin |
| GB0107510D0 (en) | 2001-03-26 | 2001-05-16 | Univ Bristol | New elongase gene and a process for the production of -9-polyunsaturated fatty acids |
| TWI324181B (en) | 2001-04-16 | 2010-05-01 | Martek Biosciences Corp | Product and process for transformation of thraustochytriales microorganisms |
| WO2004057001A2 (en) | 2002-12-19 | 2004-07-08 | University Of Bristol | Method for the production of polyunsaturated fatty acids |
| US20040172682A1 (en) | 2003-02-12 | 2004-09-02 | Kinney Anthony J. | Production of very long chain polyunsaturated fatty acids in oilseed plants |
| US7125672B2 (en) * | 2003-05-07 | 2006-10-24 | E. I. Du Pont De Nemours And Company | Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts |
| US7238482B2 (en) * | 2003-05-07 | 2007-07-03 | E. I. Du Pont De Nemours And Company | Production of polyunsaturated fatty acids in oleaginous yeasts |
| US7259255B2 (en) | 2003-06-25 | 2007-08-21 | E. I. Du Pont De Nemours And Company | Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast |
| WO2005047479A2 (en) | 2003-11-12 | 2005-05-26 | E.I. Dupont De Nemours And Company | Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast |
| US7202356B2 (en) | 2003-11-14 | 2007-04-10 | E. I. Du Pont De Nemours And Company | Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast |
| ES2529572T3 (es) | 2004-04-22 | 2015-02-23 | Commonwealth Scientific And Industrial Research Organisation | Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes |
| EP2357243B1 (en) | 2004-04-22 | 2018-12-12 | Commonwealth Scientific and Industrial Research Organisation | Synthesis of long-chain polyunsaturated fatty acids by recombinant cells |
| WO2006012326A1 (en) | 2004-06-25 | 2006-02-02 | E.I. Dupont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US7465564B2 (en) * | 2004-08-10 | 2008-12-16 | E.I. Du Pont De Nemours And Company | Production of γ-linolenic acid in oleaginous yeast |
| US7550286B2 (en) | 2004-11-04 | 2009-06-23 | E. I. Du Pont De Nemours And Company | Docosahexaenoic acid producing strains of Yarrowia lipolytica |
| AU2006318738B2 (en) | 2005-11-23 | 2011-12-22 | E. I. Du Pont De Nemours And Company | Delta-9 elongases and their use in making polyunsaturated fatty acids |
| US7943823B2 (en) * | 2006-04-28 | 2011-05-17 | E.I. Du Pont De Nemours And Company | Delta-8 desaturase and its use in making polyunsaturated fatty acids |
| US7678560B2 (en) | 2006-05-17 | 2010-03-16 | E.I. Du Pont De Nemours And Company | Δ 5 desaturase and its use in making polyunsaturated fatty acids |
| AU2007322223B2 (en) * | 2006-10-23 | 2012-12-13 | Corteva Agriscience Llc | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
| WO2008073275A2 (en) | 2006-12-07 | 2008-06-19 | E. I. Du Pont De Nemours And Company | Mutant delta-8 desaturase genes engineered by targeted mutagensis and their use in making polyunsaturated fatty acids |
| US7709239B2 (en) * | 2006-12-07 | 2010-05-04 | E.I. Du Pont De Nemours And Company | Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids |
| EP2441843A1 (en) * | 2007-02-12 | 2012-04-18 | E. I. du Pont de Nemours and Company | Production of arachidonic acid in oilseed plants |
| US8188338B2 (en) * | 2007-04-10 | 2012-05-29 | E. I. Du Pont De Nemours And Company | Delta-8 desaturases and their use in making polyunsaturated fatty acids |
-
2008
- 2008-04-09 US US12/099,799 patent/US8188338B2/en active Active
- 2008-04-09 US US12/099,811 patent/US7790156B2/en active Active
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- 2008-04-10 BR BRPI0808222-7A patent/BRPI0808222A2/pt not_active Application Discontinuation
- 2008-04-10 JP JP2010503068A patent/JP5501952B2/ja not_active Expired - Fee Related
- 2008-04-10 EP EP08745451.8A patent/EP2121737B1/en not_active Not-in-force
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- 2008-04-10 CN CN2008800187631A patent/CN101679990B/zh not_active Expired - Fee Related
- 2008-04-10 AU AU2008236595A patent/AU2008236595B2/en not_active Ceased
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2012
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| US8188338B2 (en) | 2012-05-29 |
| EP2121737A2 (en) | 2009-11-25 |
| BRPI0808222A2 (pt) | 2014-07-08 |
| EP2132317A1 (en) | 2009-12-16 |
| AU2008236595A1 (en) | 2008-10-16 |
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| EP2121737B1 (en) | 2015-05-27 |
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| AU2008237065A1 (en) | 2008-10-16 |
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| EP2132317B1 (en) | 2013-02-27 |
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| US8338152B2 (en) | 2012-12-25 |
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| US8318463B2 (en) | 2012-11-27 |
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