CA2666555A1 - Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides - Google Patents

Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides Download PDF

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Publication number
CA2666555A1
CA2666555A1 CA002666555A CA2666555A CA2666555A1 CA 2666555 A1 CA2666555 A1 CA 2666555A1 CA 002666555 A CA002666555 A CA 002666555A CA 2666555 A CA2666555 A CA 2666555A CA 2666555 A1 CA2666555 A1 CA 2666555A1
Authority
CA
Canada
Prior art keywords
group
polymerase
dna polymerase
alkenyl
combinations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002666555A
Other languages
English (en)
French (fr)
Inventor
Veeraiah Bodepudi
Amar P. Gupta
Stephen G. Will
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2666555A1 publication Critical patent/CA2666555A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002666555A 2006-10-18 2007-10-18 Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides Abandoned CA2666555A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/583,605 US7928207B2 (en) 2004-06-28 2006-10-18 Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides
US11/583,605 2006-10-18
PCT/EP2007/009019 WO2008046609A2 (en) 2006-10-18 2007-10-18 Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides

Publications (1)

Publication Number Publication Date
CA2666555A1 true CA2666555A1 (en) 2008-04-24

Family

ID=39078489

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002666555A Abandoned CA2666555A1 (en) 2006-10-18 2007-10-18 Synthesis and compositions of nucleic acids comprising 2'-terminator nucleotides

Country Status (6)

Country Link
US (1) US7928207B2 (enExample)
EP (1) EP2195327A2 (enExample)
JP (1) JP2010527580A (enExample)
CN (1) CN102083846A (enExample)
CA (1) CA2666555A1 (enExample)
WO (1) WO2008046609A2 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP2195452B1 (en) 2007-08-29 2012-03-14 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
CN102119226B (zh) * 2008-08-12 2013-10-30 霍夫曼-拉罗奇有限公司 校对引物延伸
GEP201706656B (en) 2011-11-11 2017-04-25 Pfizer 2-thiopyrimidinones
CN103266103B (zh) * 2012-11-08 2016-08-31 天津精耐特基因生物技术有限公司 用于扩增核糖核酸的方法及分析该方法中rna或dna模板的方法
US9279149B2 (en) 2013-04-02 2016-03-08 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US8808989B1 (en) * 2013-04-02 2014-08-19 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acids
US11331643B2 (en) 2013-04-02 2022-05-17 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US11384377B2 (en) 2013-04-02 2022-07-12 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9771613B2 (en) 2013-04-02 2017-09-26 Molecular Assemblies, Inc. Methods and apparatus for synthesizing nucleic acid
US10683536B2 (en) 2013-04-02 2020-06-16 Molecular Assemblies, Inc. Reusable initiators for synthesizing nucleic acids
US9856524B2 (en) * 2014-12-04 2018-01-02 Shaofeng Ding Lyophilized integrated composition for storage and manipulation of pyrophosphorolysis activated polymerization
WO2016178113A1 (en) 2015-05-05 2016-11-10 Pfizer Inc. 2-thiopyrimidinones
US10465242B2 (en) 2016-07-14 2019-11-05 University Of Utah Research Foundation Multi-sequence capture system
US11921049B2 (en) * 2018-11-09 2024-03-05 Shaofeng Ding Pyrophosphorolysis activated fluorescence to measure PAP amplification of nucleic acid
CN113950531A (zh) * 2019-05-15 2022-01-18 麻省理工学院 使用m13噬菌体优化环状单链dna
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
JPH0778074B2 (ja) * 1989-09-29 1995-08-23 堺化学工業株式会社 ヌクレオシド―２’，５’‐及び３’，５’‐二リン酸の製造方法
US5914396A (en) * 1990-01-11 1999-06-22 Isis Pharmaceuticals, Inc. 2'-O-modified nucleosides and phosphoramidites
US5859221A (en) * 1990-01-11 1999-01-12 Isis Pharmaceuticals, Inc. 2'-modified oligonucleotides
US5660985A (en) * 1990-06-11 1997-08-26 Nexstar Pharmaceuticals, Inc. High affinity nucleic acid ligands containing modified nucleotides
DE69433811T2 (de) * 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
US5532130A (en) * 1993-07-20 1996-07-02 Dyad Pharmaceutical Corporation Methods and compositions for sequence-specific hybridization of RNA by 2'-5' oligonucleotides
JP2001524808A (ja) * 1996-12-10 2001-12-04 ジーントレイス・システムズ・インコーポレイテッド 放出可能な不揮発性の質量標識分子
US6147200A (en) * 1999-08-19 2000-11-14 Isis Pharmaceuticals, Inc. 2'-O-acetamido modified monomers and oligomers
EP1409731A2 (en) * 2001-07-03 2004-04-21 ISIS Pharmaceuticals, Inc. Methods of modulating pharmacokinetics of oligonucleotides
JP3827629B2 (ja) 2002-08-30 2006-09-27 住友大阪セメント株式会社 光変調器
US7572581B2 (en) 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7947817B2 (en) * 2003-06-30 2011-05-24 Roche Molecular Systems, Inc. Synthesis and compositions of 2'-terminator nucleotides
EP1753774B1 (en) 2004-06-02 2008-09-10 ASM Scientific, Inc. 2"-nitrobenzyl-modified ribonucleotides
US7745125B2 (en) * 2004-06-28 2010-06-29 Roche Molecular Systems, Inc. 2′-terminator related pyrophosphorolysis activated polymerization
JP4753066B2 (ja) 2005-06-29 2011-08-17 独立行政法人産業技術総合研究所 イオン複合型粘土組成物およびその固化物
WO2007075967A2 (en) 2005-12-21 2007-07-05 Roche Diagnostics Gmbh Sequencing and genotyping using reversibly 2'-modified nucleotides

Also Published As

Publication number Publication date
EP2195327A2 (en) 2010-06-16
US20070219361A1 (en) 2007-09-20
WO2008046609A3 (en) 2012-09-13
WO2008046609A2 (en) 2008-04-24
US7928207B2 (en) 2011-04-19
JP2010527580A (ja) 2010-08-19
CN102083846A (zh) 2011-06-01

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FZDE Dead

Effective date: 20131128