CA2657223A1 - Use of denaturing agents during affinity capture - Google Patents
Use of denaturing agents during affinity capture Download PDFInfo
- Publication number
- CA2657223A1 CA2657223A1 CA002657223A CA2657223A CA2657223A1 CA 2657223 A1 CA2657223 A1 CA 2657223A1 CA 002657223 A CA002657223 A CA 002657223A CA 2657223 A CA2657223 A CA 2657223A CA 2657223 A1 CA2657223 A1 CA 2657223A1
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- Prior art keywords
- molecule
- affinity
- denaturing agent
- sample
- immobilized
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 229920013655 poly(bisphenol-A sulfone) Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000058 polyacrylate Polymers 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920006393 polyether sulfone Polymers 0.000 description 1
- 229920002530 polyetherether ketone Polymers 0.000 description 1
- 229920001721 polyimide Polymers 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 210000004908 prostatic fluid Anatomy 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 239000013077 target material Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US81895706P | 2006-07-07 | 2006-07-07 | |
| US60/818,957 | 2006-07-07 | ||
| PCT/US2007/072837 WO2008006017A1 (en) | 2006-07-07 | 2007-07-05 | Use of denaturing agents during affinity capture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2657223A1 true CA2657223A1 (en) | 2008-01-10 |
Family
ID=38596688
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002657223A Abandoned CA2657223A1 (en) | 2006-07-07 | 2007-07-05 | Use of denaturing agents during affinity capture |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20090209737A1 (enExample) |
| EP (1) | EP2041572A1 (enExample) |
| JP (1) | JP2009543099A (enExample) |
| CA (1) | CA2657223A1 (enExample) |
| WO (1) | WO2008006017A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2389585A2 (en) * | 2009-01-22 | 2011-11-30 | Li-Cor, Inc. | Single molecule proteomics with dynamic probes |
| KR20190107761A (ko) * | 2011-03-09 | 2019-09-20 | 셀 시그널링 테크놀러지, 인크. | 모노클로날 항체를 생성하는 방법 및 시약 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999054735A1 (en) * | 1998-04-17 | 1999-10-28 | Innogenetics N.V. | Improved immunodiagnostic assays using reducing agents |
| EP1200826B1 (en) * | 1999-08-11 | 2004-06-23 | Unilever Plc | Internally referenced immunoassay and test device |
| US20040038307A1 (en) * | 2002-05-10 | 2004-02-26 | Engeneos, Inc. | Unique recognition sequences and methods of use thereof in protein analysis |
| US7838307B2 (en) * | 2004-12-08 | 2010-11-23 | Lyotropic Therapeutics, Inc. | Compositions for binding to assay substrata and methods of using |
-
2007
- 2007-07-05 EP EP07799308A patent/EP2041572A1/en not_active Withdrawn
- 2007-07-05 JP JP2009519598A patent/JP2009543099A/ja not_active Withdrawn
- 2007-07-05 US US12/307,596 patent/US20090209737A1/en not_active Abandoned
- 2007-07-05 WO PCT/US2007/072837 patent/WO2008006017A1/en not_active Ceased
- 2007-07-05 CA CA002657223A patent/CA2657223A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2041572A1 (en) | 2009-04-01 |
| WO2008006017A1 (en) | 2008-01-10 |
| US20090209737A1 (en) | 2009-08-20 |
| JP2009543099A (ja) | 2009-12-03 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20130705 |