CA2644664C - Methods of treating, reducing and inhibiting the appearance of ageing in the skin - Google Patents
Methods of treating, reducing and inhibiting the appearance of ageing in the skin Download PDFInfo
- Publication number
- CA2644664C CA2644664C CA2644664A CA2644664A CA2644664C CA 2644664 C CA2644664 C CA 2644664C CA 2644664 A CA2644664 A CA 2644664A CA 2644664 A CA2644664 A CA 2644664A CA 2644664 C CA2644664 C CA 2644664C
- Authority
- CA
- Canada
- Prior art keywords
- skin
- subject
- appearance
- granzyme
- maintaining
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 68
- 230000032683 aging Effects 0.000 title claims abstract description 58
- 230000002401 inhibitory effect Effects 0.000 title claims abstract description 26
- 210000003491 skin Anatomy 0.000 claims abstract description 277
- 229940121832 Granzyme B inhibitor Drugs 0.000 claims abstract description 69
- 230000001965 increasing effect Effects 0.000 claims abstract description 54
- 230000037394 skin elasticity Effects 0.000 claims abstract description 14
- 108010014258 Elastin Proteins 0.000 claims description 80
- 229920002549 elastin Polymers 0.000 claims description 80
- 102000016942 Elastin Human genes 0.000 claims description 79
- 108050001049 Extracellular proteins Proteins 0.000 claims description 39
- 230000003247 decreasing effect Effects 0.000 claims description 17
- 230000037303 wrinkles Effects 0.000 claims description 16
- 239000011148 porous material Substances 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- 206010040829 Skin discolouration Diseases 0.000 claims description 7
- 206010040851 Skin fragility Diseases 0.000 claims description 7
- 230000003822 cell turnover Effects 0.000 claims description 7
- 210000004927 skin cell Anatomy 0.000 claims description 7
- 230000036620 skin dryness Effects 0.000 claims description 7
- 239000003814 drug Substances 0.000 claims description 6
- 230000037393 skin firmness Effects 0.000 claims description 5
- 210000004761 scalp Anatomy 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims description 3
- 238000007910 systemic administration Methods 0.000 claims description 2
- 230000037370 skin discoloration Effects 0.000 claims 3
- 230000000699 topical effect Effects 0.000 claims 3
- 102000001398 Granzyme Human genes 0.000 abstract description 112
- 108060005986 Granzyme Proteins 0.000 abstract description 112
- 239000003112 inhibitor Substances 0.000 abstract description 26
- 210000003780 hair follicle Anatomy 0.000 abstract description 14
- 239000000556 agonist Substances 0.000 abstract description 9
- 150000001875 compounds Chemical class 0.000 description 36
- 230000002829 reductive effect Effects 0.000 description 30
- 241000699670 Mus sp. Species 0.000 description 29
- 239000000203 mixture Substances 0.000 description 23
- 238000003776 cleavage reaction Methods 0.000 description 19
- 230000007017 scission Effects 0.000 description 19
- 238000012360 testing method Methods 0.000 description 18
- 206010040954 Skin wrinkling Diseases 0.000 description 13
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 12
- 239000006228 supernatant Substances 0.000 description 12
- 210000004209 hair Anatomy 0.000 description 9
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 108010067306 Fibronectins Proteins 0.000 description 6
- 102000016359 Fibronectins Human genes 0.000 description 6
- 206010048215 Xanthomatosis Diseases 0.000 description 6
- 239000005557 antagonist Substances 0.000 description 6
- 239000008188 pellet Substances 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 239000000523 sample Substances 0.000 description 6
- 238000001262 western blot Methods 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 210000004207 dermis Anatomy 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 230000036074 healthy skin Effects 0.000 description 5
- 239000007943 implant Substances 0.000 description 5
- 238000010186 staining Methods 0.000 description 5
- 238000007920 subcutaneous administration Methods 0.000 description 5
- 102000013918 Apolipoproteins E Human genes 0.000 description 4
- 108010035532 Collagen Proteins 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 4
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 4
- 101000817629 Homo sapiens Dymeclin Proteins 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 108010031318 Vitronectin Proteins 0.000 description 4
- 102100035140 Vitronectin Human genes 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229920001436 collagen Polymers 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 210000002615 epidermis Anatomy 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 238000000338 in vitro Methods 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000007665 sagging Methods 0.000 description 4
- 231100000257 skin discolouration Toxicity 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000007469 Actins Human genes 0.000 description 3
- 108010085238 Actins Proteins 0.000 description 3
- 108010025628 Apolipoproteins E Proteins 0.000 description 3
- 102000016387 Pancreatic elastase Human genes 0.000 description 3
- 108010067372 Pancreatic elastase Proteins 0.000 description 3
- 235000012000 cholesterol Nutrition 0.000 description 3
- 230000008045 co-localization Effects 0.000 description 3
- 230000006866 deterioration Effects 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 210000002744 extracellular matrix Anatomy 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 210000002540 macrophage Anatomy 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- -1 medicated shampoos Substances 0.000 description 3
- 230000000149 penetrating effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 235000021068 Western diet Nutrition 0.000 description 2
- 210000000709 aorta Anatomy 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000004624 confocal microscopy Methods 0.000 description 2
- 210000002808 connective tissue Anatomy 0.000 description 2
- 210000001151 cytotoxic T lymphocyte Anatomy 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 230000002500 effect on skin Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
- JJTUDXZGHPGLLC-UHFFFAOYSA-N lactide Chemical compound CC1OC(=O)C(C)OC1=O JJTUDXZGHPGLLC-UHFFFAOYSA-N 0.000 description 2
- 231100000518 lethal Toxicity 0.000 description 2
- 230000001665 lethal effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000000069 prophylactic effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 210000002374 sebum Anatomy 0.000 description 2
- 230000036548 skin texture Effects 0.000 description 2
- 239000000344 soap Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000013268 sustained release Methods 0.000 description 2
- 239000012730 sustained-release form Substances 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 230000004797 therapeutic response Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 150000003626 triacylglycerols Chemical class 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- ODJQKYXPKWQWNK-UHFFFAOYSA-N 3,3'-Thiobispropanoic acid Chemical compound OC(=O)CCSCCC(O)=O ODJQKYXPKWQWNK-UHFFFAOYSA-N 0.000 description 1
- 201000004384 Alopecia Diseases 0.000 description 1
- 108010006591 Apoenzymes Proteins 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 208000037260 Atherosclerotic Plaque Diseases 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 102100033167 Elastin Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 108010007979 Glycocholic Acid Proteins 0.000 description 1
- 108060003393 Granulin Proteins 0.000 description 1
- 206010019030 Hair colour changes Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 1
- 229930064664 L-arginine Natural products 0.000 description 1
- 235000014852 L-arginine Nutrition 0.000 description 1
- 108010028554 LDL Cholesterol Proteins 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 102000007547 Laminin Human genes 0.000 description 1
- 108010085895 Laminin Proteins 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 102000012479 Serine Proteases Human genes 0.000 description 1
- 108010022999 Serine Proteases Proteins 0.000 description 1
- 206010040799 Skin atrophy Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 235000009697 arginine Nutrition 0.000 description 1
- 230000003143 atherosclerotic effect Effects 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 208000037979 autoimmune inflammatory disease Diseases 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003518 caustics Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 210000001612 chondrocyte Anatomy 0.000 description 1
- 208000037976 chronic inflammation Diseases 0.000 description 1
- 208000037893 chronic inflammatory disorder Diseases 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 238000002316 cosmetic surgery Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 229940009976 deoxycholate Drugs 0.000 description 1
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 1
- 230000002951 depilatory effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 208000037765 diseases and disorders Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 108010064033 elastin-binding proteins Proteins 0.000 description 1
- 230000003246 elastolytic effect Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 239000005038 ethylene vinyl acetate Substances 0.000 description 1
- 230000003176 fibrotic effect Effects 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- RFDAIACWWDREDC-FRVQLJSFSA-N glycocholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 RFDAIACWWDREDC-FRVQLJSFSA-N 0.000 description 1
- 230000003648 hair appearance Effects 0.000 description 1
- 230000003803 hair density Effects 0.000 description 1
- 208000024963 hair loss Diseases 0.000 description 1
- 230000003676 hair loss Effects 0.000 description 1
- 210000004919 hair shaft Anatomy 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000007236 host immunity Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940125396 insulin Drugs 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 238000011813 knockout mouse model Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229940039092 medicated shampoos Drugs 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 150000002790 naphthalenes Chemical class 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000037311 normal skin Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 230000036470 plasma concentration Effects 0.000 description 1
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
- 229920001515 polyalkylene glycol Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 210000001732 sebaceous gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002453 shampoo Substances 0.000 description 1
- 230000037075 skin appearance Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013595 supernatant sample Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 231100001274 therapeutic index Toxicity 0.000 description 1
- 238000011200 topical administration Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/16—Emollients or protectives, e.g. against radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/007—Preparations for dry skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/10—Preparations for permanently dyeing the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96433—Serine endopeptidases (3.4.21)
- G01N2333/96436—Granzymes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pharmacology & Pharmacy (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Birds (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Epidemiology (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Cosmetics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78035206P | 2006-03-09 | 2006-03-09 | |
| US60/780,352 | 2006-03-09 | ||
| US79735206P | 2006-05-04 | 2006-05-04 | |
| US60/797,352 | 2006-05-04 | ||
| PCT/CA2007/000396 WO2007101354A1 (en) | 2006-03-09 | 2007-03-09 | Methods of treating, reducing and inhibiting the appearance of ageing in the skin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2644664A1 CA2644664A1 (en) | 2007-09-13 |
| CA2644664C true CA2644664C (en) | 2016-05-03 |
Family
ID=38474579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2644664A Expired - Fee Related CA2644664C (en) | 2006-03-09 | 2007-03-09 | Methods of treating, reducing and inhibiting the appearance of ageing in the skin |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20120171144A1 (enExample) |
| EP (1) | EP2002012A4 (enExample) |
| JP (2) | JP5746813B2 (enExample) |
| CA (1) | CA2644664C (enExample) |
| NZ (1) | NZ571858A (enExample) |
| WO (1) | WO2007101354A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2215478A4 (en) | 2007-10-01 | 2010-10-06 | Univ British Columbia | GRANZYM A AND GRANZYM B DIAGNOSTICS |
| WO2009055934A1 (en) * | 2007-11-02 | 2009-05-07 | The University Of British Columbia | Treatment of fibrillinopathy or elastinopathy using granzyme inhibitors |
| US20140056964A1 (en) * | 2010-12-06 | 2014-02-27 | The University Of British Columbia | Granzyme b inhibitor compositions, methods and uses for promoting wound healing |
| CA2908148A1 (en) * | 2013-03-29 | 2014-10-02 | Vida Therapeutics, Inc. | Indoline compounds as granzyme b inhibitors |
| US9605021B2 (en) | 2013-03-29 | 2017-03-28 | Vida Therapeutics Inc. | Indoline compounds as granzyme B inhibitors |
| JP6305727B2 (ja) * | 2013-10-31 | 2018-04-04 | ポーラ化成工業株式会社 | 皮膚光老化防止用組成物 |
| US9458193B1 (en) | 2014-08-01 | 2016-10-04 | Vida Therapeutics Inc. | Proline compounds as Granzyme B inhibitors |
| US10537652B2 (en) | 2014-08-01 | 2020-01-21 | Vida Therapeutics Inc. | Cyclic urea compounds as granzyme B inhibitors |
| US9458192B1 (en) | 2014-08-01 | 2016-10-04 | Vida Therapeutics Inc. | Covalent granzyme B inhibitors |
| WO2016015159A1 (en) | 2014-08-01 | 2016-02-04 | Vida Therapeutics, Inc. | Azaindoline compounds as granzyme b inhibitors |
| US9458138B1 (en) | 2014-08-01 | 2016-10-04 | viDATherapeutics Inc. | Pyrrole compounds as granzyme B inhibitors |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000136122A (ja) * | 1998-10-28 | 2000-05-16 | Kose Corp | 皮膚外用剤 |
| EP1163900A1 (en) * | 1998-11-05 | 2001-12-19 | Protease Sciences, Incorporated | Cosmetic compositions containing human type serine protease inhibitors |
| EP1162934A4 (en) * | 1999-02-22 | 2005-01-26 | Eric F Bernstein | COMPOSITIONS AND METHODS FOR PREVENTING PHOTOVAGENING |
| US6699486B1 (en) * | 1999-11-18 | 2004-03-02 | Bolla Corporation | Treatment or prevention of photoaging and skin cancer |
| US7326692B2 (en) * | 2001-11-14 | 2008-02-05 | The University Of Chicago | Induction of immunity using inhibitors of granzymes |
| JP2005522430A (ja) * | 2002-02-04 | 2005-07-28 | メルク エンド カムパニー インコーポレーテッド | グランザイムｂ阻害剤 |
| JP2004075661A (ja) * | 2002-06-18 | 2004-03-11 | Shiseido Co Ltd | 表皮基底膜ケアを特徴とする皮膚外用剤、皮膚基底膜構造形成促進剤および人工皮膚の製造方法 |
-
2007
- 2007-03-09 US US12/282,157 patent/US20120171144A1/en not_active Abandoned
- 2007-03-09 JP JP2008557569A patent/JP5746813B2/ja not_active Expired - Fee Related
- 2007-03-09 CA CA2644664A patent/CA2644664C/en not_active Expired - Fee Related
- 2007-03-09 WO PCT/CA2007/000396 patent/WO2007101354A1/en not_active Ceased
- 2007-03-09 EP EP07710727A patent/EP2002012A4/en not_active Ceased
- 2007-03-09 NZ NZ571858A patent/NZ571858A/xx not_active IP Right Cessation
-
2013
- 2013-05-28 JP JP2013112324A patent/JP2013173794A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2009532332A (ja) | 2009-09-10 |
| AU2007222865A1 (en) | 2007-09-13 |
| EP2002012A4 (en) | 2011-01-05 |
| NZ571858A (en) | 2013-08-30 |
| JP5746813B2 (ja) | 2015-07-08 |
| CA2644664A1 (en) | 2007-09-13 |
| US20120171144A1 (en) | 2012-07-05 |
| JP2013173794A (ja) | 2013-09-05 |
| WO2007101354A1 (en) | 2007-09-13 |
| EP2002012A1 (en) | 2008-12-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2644664C (en) | Methods of treating, reducing and inhibiting the appearance of ageing in the skin | |
| AU2020273346B2 (en) | Methods and compositions for topical delivery for skin care | |
| TWI862641B (zh) | 具有抗老化功效之多肽及其用途 | |
| EP2961481B1 (fr) | Composition dermatologique antimicrobienne topique | |
| KR20230065274A (ko) | 조직 유래된 매트리킨 조성물 및 이에 대한 방법 | |
| CN112979762B (zh) | 环肽piz及其用途 | |
| AU2007222865B2 (en) | Methods of treating, reducing and inhibiting the appearance of ageing in the skin | |
| US8846064B2 (en) | Cosmetic and pharmaceutical compositions having modified proteins in the form of a supramolecular assembly | |
| CN115515622A (zh) | 用于皮肤状况的衍生自乙酰胆碱酯酶c-末端的多肽 | |
| US20250255792A1 (en) | Methods of Using a Small Molecule Chemical Compound to Reduce the Appearance of Rhytids and Wrinkles | |
| JP2025529453A (ja) | 皺皮および皺の外観を軽減するために低分子の化学化合物を使用する方法 | |
| US20240207373A1 (en) | Cyclic peptides from the c-terminus of acetylcholinesterase for treatment of skin disorders and for cosmetical use | |
| WO2025259703A1 (en) | Combinations of cocoa polyphenols and amino acids for personal care or treatment, and compositions and methods thereof | |
| TW201924656A (zh) | 用於調節黑色素生成的方法及組成物 | |
| TW201334803A (zh) | 肝素用於皮膚保濕與美白及防止皮膚老化之新穎用途 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20220909 |
|
| MKLA | Lapsed |
Effective date: 20210309 |