CA2629284A1 - Transformation de brassica par bombardement de microprojectiles - Google Patents

Transformation de brassica par bombardement de microprojectiles Download PDF

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Publication number
CA2629284A1
CA2629284A1 CA002629284A CA2629284A CA2629284A1 CA 2629284 A1 CA2629284 A1 CA 2629284A1 CA 002629284 A CA002629284 A CA 002629284A CA 2629284 A CA2629284 A CA 2629284A CA 2629284 A1 CA2629284 A1 CA 2629284A1
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CA
Canada
Prior art keywords
microspore
plant
brassica
bombardment
embryo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002629284A
Other languages
English (en)
Other versions
CA2629284C (fr
Inventor
Wenpin Chen
Lomas Tulsieram
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pioneer Hi Bred International Inc
Original Assignee
Pioneer Hi-Bred International, Inc.
Wenpin Chen
Lomas Tulsieram
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi-Bred International, Inc., Wenpin Chen, Lomas Tulsieram filed Critical Pioneer Hi-Bred International, Inc.
Publication of CA2629284A1 publication Critical patent/CA2629284A1/fr
Application granted granted Critical
Publication of CA2629284C publication Critical patent/CA2629284C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2629284A 2005-11-10 2005-11-10 Transformation de brassica par bombardement de microprojectiles Active CA2629284C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2005/040646 WO2007055687A1 (fr) 2005-11-10 2005-11-10 Transformation de brassica par bombardement de microprojectiles

Publications (2)

Publication Number Publication Date
CA2629284A1 true CA2629284A1 (fr) 2007-05-18
CA2629284C CA2629284C (fr) 2018-05-01

Family

ID=36604596

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2629284A Active CA2629284C (fr) 2005-11-10 2005-11-10 Transformation de brassica par bombardement de microprojectiles

Country Status (6)

Country Link
EP (1) EP1945774A1 (fr)
CN (1) CN101356276A (fr)
AU (1) AU2005338055B2 (fr)
CA (1) CA2629284C (fr)
EA (1) EA200801303A1 (fr)
WO (1) WO2007055687A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017513506A (ja) * 2014-04-28 2017-06-01 ダウ アグロサイエンシィズ エルエルシー ハプロイドトウモロコシ形質転換

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051756A (en) * 1998-02-26 2000-04-18 Cargill, Incorporated Particle bombardment transformation of Brassica
US6297056B1 (en) * 1999-10-12 2001-10-02 Pioneer Hi-Bred International, Inc. Brassica transformation via microprojectile bombardment

Also Published As

Publication number Publication date
EA200801303A1 (ru) 2009-02-27
AU2005338055B2 (en) 2012-02-02
AU2005338055A1 (en) 2007-05-18
EP1945774A1 (fr) 2008-07-23
WO2007055687A1 (fr) 2007-05-18
CN101356276A (zh) 2009-01-28
CA2629284C (fr) 2018-05-01

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