CA2568805A1 - Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis - Google Patents

Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis Download PDF

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Publication number
CA2568805A1
CA2568805A1 CA002568805A CA2568805A CA2568805A1 CA 2568805 A1 CA2568805 A1 CA 2568805A1 CA 002568805 A CA002568805 A CA 002568805A CA 2568805 A CA2568805 A CA 2568805A CA 2568805 A1 CA2568805 A1 CA 2568805A1
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Prior art keywords
wetting
layer
track
electrodes
zones
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Granted
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CA002568805A
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French (fr)
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CA2568805C (en
Inventor
Jean-Christophe Fourrier
Francois Caron
Pierre Tabourier
Christian Druon
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Centre National de la Recherche Scientifique CNRS
Universite de Lille 1 Sciences et Technologies
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Individual
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
    • F04B19/006—Micropumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/088—Passive control of flow resistance by specific surface properties
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F05—INDEXING SCHEMES RELATING TO ENGINES OR PUMPS IN VARIOUS SUBCLASSES OF CLASSES F01-F04
    • F05C—INDEXING SCHEME RELATING TO MATERIALS, MATERIAL PROPERTIES OR MATERIAL CHARACTERISTICS FOR MACHINES, ENGINES OR PUMPS OTHER THAN NON-POSITIVE-DISPLACEMENT MACHINES OR ENGINES
    • F05C2225/00—Synthetic polymers, e.g. plastics; Rubber
    • F05C2225/04—PTFE [PolyTetraFluorEthylene]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Micromachines (AREA)

Abstract

A device for handling drops on a displacement by electrowetting plane, including at least one displacement track. The track includes an electrically insulating substrate on the surface of which rest two or more interdigitated conducting electrodes. These electrodes are covered by a dielectric insulating layer, itself covered by a partially-wetting layer. Also, a method for the manufacture of the aforementioned device, in which the creation of the partially-wetting layer includes the creation of a mask in a photosensitive material by the deposition of this material onto a substrate, then a photolithographic stage, followed by development of the photosensitive material, the deposition of a non-wetting material onto the mask, at least one annealing process before dissolution, dissolution of the mask, and at least one annealing process after dissolution. Also, a system for the microfluidic analysis of a liquid sample.

Claims (26)

1. Dispositif de manipulation de gouttes sur un plan de déplacement par électromouillage, comprenant au moins une piste, caractérisé
en ce que ladite piste comprend :
- un substrat électriquement isolant présentant une surface supérieure, - au moins deux premières électrodes conductrices présentant une surface supérieure et une surface inférieure, reposant par leur surface inférieure sur ladite surface supérieure dudit substrat électriquement isolant, chacune desdites premières électrodes étant interdigitées avec au moins une autre de ces dites premières électrodes, - une couche diélectrique isolante présentant une surface inférieure et une surface supérieure, reposant par sa surface inférieure sur ladite surface supérieure desdites premières électrodes, - une couche partiellement mouillante présentant une surface inférieure et une surface supérieure, reposant par sa surface inférieure sur la surface supérieure de la dite couche diélectrique isolante. 1. Device for handling drops on a plane of movement by electrowetting, comprising at least one track, characterized in that said track comprises:
an electrically insulating substrate having a surface top, at least two first conductive electrodes having an upper surface and a lower surface, resting by their lower surface on said upper surface of said electrically insulating substrate, each of said first electrodes being interdigitated with at least one of these say first electrodes, an insulating dielectric layer having a surface lower and an upper surface, resting by its surface lower on said upper surface of said first electrodes, a partially wetting layer having a surface lower and an upper surface, resting by its surface lower on the upper surface of the said layer insulating dielectric. 2. Dispositif selon la revendication 1, caractérisé en ce qu'il comprend au moins une contre-électrode distincte desdites premières électrodes. 2. Device according to claim 1, characterized in that it comprises at least one counter-electrode separate from said first electrodes. 3. Dispositif selon la revendication 2, caractérisé en ce que ladite contre-électrode distincte est une ligne de masse située sur ou sous la surface supérieure de ou insérée dans ladite couche partiellement mouillante. 3. Device according to claim 2, characterized in that said distinct counter-electrode is a ground line located on or under the upper surface of or inserted into said layer partially wetting. 4. Dispositif selon l'une quelconque des revendications 1 à 3, caractérisé en ce qu'il comprend une deuxième piste positionnée de façon opposée à et séparée de la première piste de telle sorte qu'un espace est formé entre lesdites première et deuxième pistes, ladite deuxième piste comprenant une couche non mouillante présentant une surface inférieure du côté dudit espace et une surface supérieure de l'autre côté. 4. Device according to any one of claims 1 to 3, characterized in that it comprises a second track positioned opposite to and separate from the first track so that a space is formed between said first and second tracks, said second track comprising a non-wetting layer having a lower surface on the side of said space and a upper surface on the other side. 5. Dispositif selon la revendication 4, caractérisé en ce que ladite couche non mouillante de ladite deuxième piste est partiellement mouillante. 5. Device according to claim 4, characterized in that said non-wetting layer of said second track is partially wetting. 6. Dispositif selon l'une quelconque des revendications 4 et 5, caractérisé en ce que ladite deuxième piste comprend une couche supérieure électriquement isolante, semi-conductrice ou conductrice, située du côté de la surface supérieure de ladite couche non mouillante. 6. Device according to any one of claims 4 and 5, characterized in that said second track comprises a layer superior electrically insulating, semiconducting or conductor, located on the side of the upper surface of said non-wetting layer. 7. Dispositif selon l'une quelconque des revendications 4 à 6, caractérisé en ce que ladite deuxième piste comprend une ou plusieurs contre-électrodes situées entre ladite couche non mouillante et ladite couche supérieure. 7. Device according to any one of claims 4 to 6, characterized in that said second track comprises one or several counter electrodes located between said non wetting agent and said upper layer. 8. Dispositif selon la revendication 7, caractérisé en ce que ladite deuxième piste comprend une couche diélectrique isolante située entre ladite couche non mouillante et la ou les dites contre-électrodes. 8. Device according to claim 7, characterized in that said second runway includes an insulating dielectric layer located between said non-wetting layer and said one or more electrodes. 9. Dispositif selon l'une quelconque des revendications 1 à 8, caractérisé en ce que ladite couche partiellement mouillante de ladite première piste et/ou de ladite deuxième piste comprend des zones non mouillantes et des zones mouillantes, lesdites zones mouillantes étant des zones fonctionnalisées réactives. 9. Device according to any one of claims 1 to 8, characterized in that said partially wetting layer of said first track and / or said second track comprises non-wetting zones and wetting zones, the said zones wetting agents being reactive functionalized areas. 10. Dispositif de manipulation de gouttes entre deux plans de déplacement par électromouillage, comprenant deux pistes séparées par un espace, caractérisé en ce que :
- la première piste comprend :
i. un substrat électriquement isolant présentant une surface supérieure, ii. au moins deux premières électrodes présentant une surface supérieure et une surface inférieure, reposant par leur surface inférieure sur ladite surface supérieure dudit substrat électriquement isolant, chacune desdites premières électrodes étant interdigitées avec au moins une autre de ces dites premières électrodes, iii. une couche non mouillante présentant une surface inférieure et une surface supérieure, située du côté de la surface supérieure desdites premières électrodes, - la deuxième piste comprend une couche partiellement mouillante présentant une surface supérieure et une surface inférieure, ladite couche partiellement mouillante de ladite première piste et/ou de ladite deuxième piste comprenant des zones non mouillantes et des zones mouillantes, lesdites zones mouillantes étant des zones fonctionnalisées réactives. 10. Device for handling drops between two planes traveling by electrowetting, comprising two tracks separated by a space, characterized in that:
- the first track includes:
i. an electrically insulating substrate having a surface top, ii. at least two first electrodes having a upper surface and a lower surface, resting by their lower surface on said upper surface of said electrically insulating substrate, each of said first electrodes being interdigitated with at least another of these said first electrodes, iii. a non-wetting layer having a surface lower and an upper surface, located on the side of the upper surface of said first electrodes, - the second track includes a layer partially wetting having a top surface and a surface lower said partially wetting layer of said first track and / or of said second track comprising non-wetting zones and wetting zones, said wetting zones being zones reactive functionalized. 11. Dispositif selon la revendication 10, caractérisé en ce que ladite première piste comprend une couche diélectrique isolante située entre la surface supérieure desdites premières électrodes et la surface inférieure de ladite couche non mouillante. 11. Device according to claim 10, characterized in that said first track includes an insulating dielectric layer located between the upper surface of said first electrodes and the lower surface of said non-wetting layer. 12. Dispositif selon l'une quelconque des revendications 10 et 11, caractérisé en ce qu'il comprend une ligne de masse située sur ou sous la surface supérieure de ou insérée dans ladite couche non mouillante. 12. Device according to any one of claims 10 and 11, characterized in that it comprises a ground line located on or under the top surface of or inserted into said non-layer wetting. 13. Dispositif selon l'une quelconque des revendications 10 à 12, caractérisé en ce que ladite deuxième piste comprend une couche électriquement isolante, conductrice ou semi-conductrice, située du côté de la surface supérieure de ladite couche non mouillante. 13. Device according to any one of claims 10 to 12, characterized in that said second track comprises a layer electrically insulating, conductive or semiconductive side of the upper surface of said non-wetting layer. 14. Dispositif selon l'une quelconque des revendications 1 à 13, caractérisé en ce que ledit substrat électriquement isolant de ladite première piste est transparent. 14. Device according to any one of claims 1 to 13, characterized in that said electrically insulating substrate of said first track is transparent. 15. Dispositif selon la revendication 14, caractérisé en ce que ledit substrat électriquement isolant de ladite première piste est un substrat de verre. 15. Device according to claim 14, characterized in that said electrically insulating substrate of said first track is a glass substrate. 16. Dispositif selon l'une quelconque des revendications 9 à 15, caractérisé en ce que lesdites zones mouillantes sont des ouvertures dans des zones non mouillantes. Device according to one of claims 9 to 15, characterized in that said wetting zones are openings in non-wetting areas. 17. Dispositif selon l'une quelconque des revendications 9 et 16, caractérisé en ce que lesdites zones mouillantes sont biochimiquement fonctionnalisées et réactives 17. Device according to any one of claims 9 and 16, characterized in that said wetting zones are biochemically functionalized and reactive 18. Dispositif selon l'une quelconque des revendications 1 à 17, caractérisé en ce que ladite couche non mouillante et/ou lesdites zones non mouillantes de ladite couche partiellement mouillante, sont non mouillantes vis à vis de l'eau, donc hydrophobes, et en ce que lesdites zones mouillantes sont mouillantes vis à vis de l'eau, donc hydrophiles. 18. Device according to any one of claims 1 to 17, characterized in that said non-wetting layer and / or said non-wetting areas of said partially wetting layer, are non-wetting with respect to water, therefore hydrophobic, and in that that said wetting zones are wetting with respect to water, therefore hydrophilic. 19. Dispositif selon l'une quelconque des revendications 1 à 18, caractérisé en ce que ladite couche non mouillante et/ou lesdites zones non mouillantes de ladite couche partiellement mouillante sont en polymère de tétrafluoroéthylène. 19. Device according to any one of claims 1 to 18, characterized in that said non-wetting layer and / or said non-wetting areas of said partially wetting layer are made of tetrafluoroethylene polymer. 20. Procédé de fabrication du dispositif selon l'une quelconque des revendications 1 à 29, dans lequel la création de ladite couche partiellement mouillante de ladite première piste ou ladite deuxième piste comprend :
- une étape de création d'un masque en matériau photosensible, par dépôt dudit matériau photosensible sur un substrat, photolithographie, puis révélation dudit matériau photosensible, - une étape de dépôt d'un matériau non mouillant sur ledit masque, - au moins une étape de recuit avant dissolution, - une étape de dissolution dudit masque, - au moins une étape de recuit après dissolution. 20. A method of manufacturing the device according to any one of Claims 1 to 29, wherein the creation of said layer partially wetting of said first track or said second track includes:
a step of creating a mask made of material photosensitive, by depositing said photosensitive material on a substrate, photolithography, then revealing said material photosensitive, a step of depositing a non-wetting material on said mask, at least one annealing step before dissolution, a step of dissolving said mask, at least one annealing step after dissolution. 21. Procédé selon la revendication 20, caractérisé en ce que la température de recuit de ladite étape de recuit avant dissolution est inférieure à la température de recuit de ladite étape de recuit après dissolution. 21. Method according to claim 20, characterized in that the annealing temperature of said annealing step before dissolution is lower than the annealing temperature of said annealing step after dissolution. 22. Procédé selon l'une quelconque des revendications 20 et 21, caractérisé en ce que ladite étape de dépôt d'un matériau non mouillant sur ledit masque, est une étape de dépôt d'un polymère de tétrafluoroétylène. 22. Process according to any one of claims 20 and 21, characterized in that said step of depositing a non-material wetting on said mask, is a step of depositing a polymer of tetrafluoroetylene. 23. Système d'analyse microfluidique d'un échantillon de liquide caractérisé en ce qu'il comprend :
- au moins un moyen de préparation de l'échantillon de liquide présentant au moins une sortie, - au moins un dispositif de manipulation de gouttes selon l'une quelconque des revendications 1 à 19, couplé par une de ses entrées à l'une des sorties dudit moyen de préparation, et présentant au moins une sortie, - au moins un moyen d'analyse couplé par une de ses entrées à l'une des sorties dudit dispositif de manipulation de gouttes. 23. Microfluidic analysis system of a liquid sample characterized in that it comprises:
at least one means for preparing the liquid sample having at least one exit, at least one drop handling device according to one of any of claims 1 to 19, coupled by one of his inputs to one of the outputs of said preparation means, and having at least one exit, at least one analysis means coupled by one of its inputs at one of the outputs of said drop handling device. 24. Système selon la revendication 23, caractérisé en ce ledit moyen de préparation comprend un ou plusieurs réservoirs ou quais de chargement. 24. System according to claim 23, characterized in that said means of preparation includes one or more tanks or docks loading. 25. Système selon l'une quelconque des revendications 23 et 24, caractérisé en ce que ledit moyen d'analyse est un spectromètre de masse, ou un détecteur de fluorescence, ou un détecteur d'émissions UV. 25. System according to any one of claims 23 and 24, characterized in that said analyzing means is a spectrometer of mass, or a fluorescence detector, or an emissions detector UV. 26. Système selon l'une quelconque des revendications 23 à 25, caractérisé en ce qu'il est intégré dans un micro-laboratoire. 26. System according to any one of claims 23 to 25, characterized in that it is integrated in a micro-laboratory.
CA2568805A 2004-06-04 2005-06-06 Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis Expired - Fee Related CA2568805C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0406080A FR2871150B1 (en) 2004-06-04 2004-06-04 DROP HANDLING DEVICE FOR BIOCHEMICAL ANALYSIS, DEVICE MANUFACTURING METHOD, AND MICROFLUIDIC ANALYSIS SYSTEM
FR0406080 2004-06-04
PCT/FR2005/001385 WO2006003293A2 (en) 2004-06-04 2005-06-06 Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis

Publications (2)

Publication Number Publication Date
CA2568805A1 true CA2568805A1 (en) 2006-01-12
CA2568805C CA2568805C (en) 2012-08-28

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CA2568805A Expired - Fee Related CA2568805C (en) 2004-06-04 2005-06-06 Device for handling drops for biochemical analysis, method for producing said device and a system for microfludic analysis

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US (1) US20080110753A1 (en)
EP (1) EP1781409B1 (en)
JP (1) JP4763690B2 (en)
KR (1) KR101179411B1 (en)
CN (1) CN101031362B (en)
AT (1) ATE540756T1 (en)
CA (1) CA2568805C (en)
FR (1) FR2871150B1 (en)
WO (1) WO2006003293A2 (en)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2871076A1 (en) * 2004-06-04 2005-12-09 Univ Lille Sciences Tech DEVICE FOR LASER RADIATION DESORPTION INCORPORATING HANDLING OF THE LIQUID SAMPLE IN THE FORM OF INDIVIDUAL DROPS ENABLING THEIR CHEMICAL AND BIOCHEMICAL TREATMENT
JP4632300B2 (en) * 2005-02-14 2011-02-16 国立大学法人 筑波大学 Liquid feeding device
JP4893197B2 (en) * 2006-09-28 2012-03-07 ブラザー工業株式会社 Liquid transfer device
WO2009032863A2 (en) * 2007-09-04 2009-03-12 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
JP5511669B2 (en) 2007-10-02 2014-06-04 セラノス， インコーポレイテッド Modular point-of-care device and use thereof
JP5383138B2 (en) * 2008-10-01 2014-01-08 シャープ株式会社 Liquid feeding structure with electrowetting valve, microanalysis chip and analyzer using the same
US8877512B2 (en) * 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8734628B2 (en) 2010-03-10 2014-05-27 Empire Technology Development, Llc Microfluidic channel device with array of drive electrodes
WO2011137533A1 (en) 2010-05-05 2011-11-10 The Governing Council Of The University Of Toronto Method of processing dried samples using digital microfluidic device
CN101865928B (en) * 2010-05-06 2012-07-18 大连理工大学 Super-hydrophobic surface micro-droplet operation and control method based on electric field effect
JP4949506B2 (en) * 2010-07-16 2012-06-13 シャープ株式会社 Channel structure, method for manufacturing the same, analysis chip, and analysis apparatus
US9476811B2 (en) * 2010-10-01 2016-10-25 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
JP2012150098A (en) * 2010-12-28 2012-08-09 Sharp Corp Chip for detecting specimen, sensor using the same, and method for detecting specimen
CN106290159A (en) 2011-01-21 2017-01-04 提拉诺斯公司 Sample uses maximized system and method
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
WO2013052318A1 (en) * 2011-09-25 2013-04-11 Theranos, Inc. Systems and methods for multi-analysis
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
CN102824933B (en) * 2012-09-20 2014-09-03 复旦大学 Digital micro-current chip electrode configuration for single drop transportation
US9366647B2 (en) * 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
US10422806B1 (en) 2013-07-25 2019-09-24 Theranos Ip Company, Llc Methods for improving assays of biological samples
TWI507690B (en) * 2014-09-02 2015-11-11 Silicon Optronics Inc Biochip package
US20200390811A1 (en) * 2015-04-23 2020-12-17 The Trustees Of The University Of Pennsylvania Compositions to disrupt protein kinase a anchoring and uses thereof
US10695762B2 (en) 2015-06-05 2020-06-30 Miroculus Inc. Evaporation management in digital microfluidic devices
US10464067B2 (en) 2015-06-05 2019-11-05 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
EP3344388B1 (en) * 2015-09-02 2020-11-11 Tecan Trading AG Magnetic conduits in microfluidics
WO2018039281A1 (en) 2016-08-22 2018-03-01 Miroculus Inc. Feedback system for parallel droplet control in a digital microfluidic device
EP3563151A4 (en) 2016-12-28 2020-08-19 Miroculus Inc. Digital microfluidic devices and methods
US11623219B2 (en) 2017-04-04 2023-04-11 Miroculus Inc. Digital microfluidics apparatuses and methods for manipulating and processing encapsulated droplets
CN110892258A (en) 2017-07-24 2020-03-17 米罗库鲁斯公司 Digital microfluidic system and method with integrated plasma collection device
CN115582155A (en) 2017-09-01 2023-01-10 米罗库鲁斯公司 Digital microfluidic device and method of use thereof
CN112469504A (en) 2018-05-23 2021-03-09 米罗库鲁斯公司 Control of evaporation in digital microfluidics
WO2020175083A1 (en) * 2019-02-25 2020-09-03 国立研究開発法人産業技術総合研究所 Open space type liquid manipulating device
CA3133124A1 (en) 2019-04-08 2020-10-15 Miroculus Inc. Multi-cartridge digital microfluidics apparatuses and methods of use
WO2021016614A1 (en) 2019-07-25 2021-01-28 Miroculus Inc. Digital microfluidics devices and methods of use thereof
CN111804354B (en) 2020-04-07 2021-09-21 苏州大学 Liquid drop nondestructive transfer device and method, and liquid drop micro-reaction method
US11857961B2 (en) 2022-01-12 2024-01-02 Miroculus Inc. Sequencing by synthesis using mechanical compression

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3361530B2 (en) * 1996-06-28 2003-01-07 カリパー・テクノロジーズ・コープ． Electronic pipettor and compensation means for electrophoretic bias
JP3791999B2 (en) * 1997-03-24 2006-06-28 株式会社アドバンス Liquid particle handling equipment
JP3829491B2 (en) * 1998-08-27 2006-10-04 株式会社日立製作所 Probe tip, probe tip creation method, sample detection method, and sample detection device
US6565727B1 (en) * 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
JP2002027984A (en) * 2000-07-17 2002-01-29 Mitsubishi Chemicals Corp Microreactor chip, method for testing chemical reaction, and thin film material for microreator chip
JP2002031638A (en) * 2000-07-17 2002-01-31 Mitsubishi Chemicals Corp Chip and method for detection of living body sample
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
JP2003222611A (en) * 2001-11-20 2003-08-08 Nec Corp Separating apparatus and method therefor, and manufacturing method thereof
AU2002359508A1 (en) * 2001-11-26 2003-06-10 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
JP2003230829A (en) * 2001-12-06 2003-08-19 Hitachi Ltd Plane microfactory
US7459127B2 (en) * 2002-02-26 2008-12-02 Siemens Healthcare Diagnostics Inc. Method and apparatus for precise transfer and manipulation of fluids by centrifugal and/or capillary forces
JP2003294733A (en) * 2002-03-29 2003-10-15 Sumitomo Bakelite Co Ltd Identifying method of protein quantity in cell, and substrate
JP3914806B2 (en) * 2002-04-09 2007-05-16 三菱化学株式会社 Analysis chip
KR100455293B1 (en) * 2002-05-15 2004-11-06 삼성전자주식회사 A process for producing array plate for a biomolecule comprising a hydrophilic region and a hydrophobic region
JPWO2004008132A1 (en) * 2002-07-11 2005-11-10 三菱電機株式会社 Biomolecule separation cell, method for producing the same, and DNA sorting apparatus
JP2004061229A (en) * 2002-07-26 2004-02-26 Hitachi Ltd Infectious disease inspecting apparatus, inspection method, and micro fabrication for inspecting infection
US6989234B2 (en) * 2002-09-24 2006-01-24 Duke University Method and apparatus for non-contact electrostatic actuation of droplets
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
JP4590542B2 (en) * 2003-06-10 2010-12-01 国立大学法人九州工業大学 Micro droplet transport device
JP4385124B2 (en) * 2004-03-12 2009-12-16 国立大学法人九州工業大学 Electrically controllable microdroplet transport device
FR2871076A1 (en) * 2004-06-04 2005-12-09 Univ Lille Sciences Tech DEVICE FOR LASER RADIATION DESORPTION INCORPORATING HANDLING OF THE LIQUID SAMPLE IN THE FORM OF INDIVIDUAL DROPS ENABLING THEIR CHEMICAL AND BIOCHEMICAL TREATMENT
US20060266700A1 (en) * 2005-05-31 2006-11-30 General Electric Company Porous structures with engineered wettability properties and methods of making them

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