CA2531972A1 - Sample presentation device with differing wettability - Google Patents

Sample presentation device with differing wettability Download PDF

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Publication number
CA2531972A1
CA2531972A1 CA002531972A CA2531972A CA2531972A1 CA 2531972 A1 CA2531972 A1 CA 2531972A1 CA 002531972 A CA002531972 A CA 002531972A CA 2531972 A CA2531972 A CA 2531972A CA 2531972 A1 CA2531972 A1 CA 2531972A1
Authority
CA
Canada
Prior art keywords
sample
analytes
presentation device
zone
zones
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002531972A
Other languages
French (fr)
Other versions
CA2531972C (en
Inventor
Mark L. Stolowitz
Christopher M. Belisle
Donald P. Paquin
John A. Ii Walker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen Sciences LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2531972A1 publication Critical patent/CA2531972A1/en
Application granted granted Critical
Publication of CA2531972C publication Critical patent/CA2531972C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0431—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components for liquid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0819—Microarrays; Biochips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.

Claims (42)

1. A sample presentation device for detecting analytes in a sample comprising a substrate having a surface, wherein the surface is comprised of a plurality of zones of differing wettability, and wherein the zone from which analytes in the sample are detected is substantially analyte binding resistant.
2. The sample presentation device of claim 1, wherein one of the zones of differing wettability is optimal with respect to retention of the sample.
3. The sample presentation device of claim 1, wherein one of the zones of differing wettability is optimal with respect to high sensitivity detection of the analytes.
4. The sample presentation device of claim 1, wherein the substrate is selected from one or more of the group consisting of glasses, semiconductors, metals, polymers, plastics, SiO2 on silicon, and Al2O3 on aluminum.
5. The sample presentation device of claim 1, wherein one or more of the zones of differing wettability is comprised of self-assembled monolayers.
6. The sample presentation device of claim 1, further comprising a boundary zone that is substantially nonwettable and one or more additional zones, each of which is more wettable than the boundary zone.
7. The sample presentation device of claim 6, wherein the one or more additional zones comprise a liquid retention zone that is more wettable than the boundary zone, and an analysis zone that is more wettable than the liquid retention zone.
8. The sample presentation device of claim 6, wherein the boundary zone has a higher contact angle than each zone of the one or more additional zones.
9. The sample presentation device of claim 7, wherein the boundary zone has a higher contact angle than the liquid retention zone, and wherein the liquid retention zone has a higher contact angle than the analysis zone.
10. The sample presentation device of claim 1, wherein the plurality of zones of differing wettability comprise a boundary zone that is substantially nonwettable, and at least one wettable zone that substantially binds analytes.
11. A sample presentation device for storing analytes from a sample comprising a substrate having a surface, wherein the surface is comprised of a plurality of zones of differing wettability, and wherein the zone in which the analytes from the sample are stored is substantially analyte binding resistant.
12. A sample presentation device for storing analytes from a sample comprising a substrate having a surface, wherein the surface is comprised of a plurality of zones of differing wettability, and wherein the zone in which the analytes from the sample are stored substantially binds analytes.
13. The sample presentation device of claim 1, wherein the sample is less than or equal to 100 µL in volume.
14. The sample presentation device of claim 1, wherein the sample is less than or equal to 70 µL in volume.
15. A method of making a sample presentation device for detecting analytes in a sample, wherein the sample presentation device comprises a substrate having a surface, comprising modifying the surface of the substrate to create a plurality of zones of differing wettability, and wherein the zone from which the analytes in the sample are detected is substantially analyte binding resistant.
16. The method of claim 15, wherein modifying comprises applying one or more self-assembled monolayers to the surface of the substrate.
17. The method of claim 16, wherein applying the self-assembled monolayers comprises patterning the surface with a patterning technique selected from one or more of the group consisting of W photo-patterning, photolithographic patterning, microstamping electron-beam patterning, and reactive-ion etching.
18. A method of detecting analytes in a sample, comprising contacting the sample with the sample presentation device of claim 1, and detecting analytes in the sample.
19. A method of detecting analytes in a plurality of samples, comprising contacting the plurality of samples with the sample presentation device of claim 1, and detecting analytes in the plurality of samples.
20. The method of claim 18, wherein detecting analytes in the sample comprises one of the group consisting of mass spectrometry, surface plasmon resonance, fluorescence, atomic force microscopy, optical spectroscopy, bioluminescence, chemiluminescence, x-ray photoelectron spectroscopy, ellipsometry, electrochemical detection, phosphorescence, ultraviolet spectroscopy, visible spectroscopy, and infrared spectroscopy.
21. The method of claim 20, wherein the mass spectrometry is laser desorption ionization mass spectrometry.
22. A method of concentrating a sample containing analytes using the sample presentation device of claim 1, comprising concentrating the sample in a zone of highest degree of wettability.
23. The method of claim 22, wherein the zone of highest degree of wettability is less than 2 mm2 in area.
24. The method of claim 22, wherein the zone of highest degree of wettability is less than 1 mm2 in area.
25. The method of claim 22, further comprising transferring the sample concentrated in the zone of highest degree of wettability to one or more additional sample presentation devices, each device comprising a plurality of zones of differing wettability with respect to the concentrated sample.
26. A method of detecting analytes in a sample, comprising capturing from the sample analytes that bind substantially to one or more zones of the sample presentation device of claim 10.
27. A method of detecting analytes in a sample, comprising depleting from the sample substances that interfere with subsequent sample handling processes, wherein the substances bind substantially to one or more zones of the sample presentation device of claim 10.
28. A method of storing analytes from a sample, comprising storing the analytes on a sample presentation device, wherein the sample presentation device comprises a substrate having a surface, wherein the surface is comprised of a plurality of zones of differing wettability and wherein the zone in which the analytes from the sample are stored is substantially analyte binding resistant.
29. A method of storing analytes from a sample, comprising storing analytes on a sample presentation device, wherein the sample presentation device comprises a substrate having a surface, wherein the surface is comprised of a plurality of zones of differing wettability, and wherein the zone in which the analytes from the sample are stored substantially binds analytes.
30. A method of handling a sample containing analytes comprising, contacting the sample with a sample presentation device comprised of a plurality of zones of differing wettability, concentrating the sample in the zone of highest degree of wettability, and wherein the zone with the highest degree of wettabilty is substantially analyte binding resistant.
31. A method of handling a sample containing analytes comprising, contacting the sample with a sample presentation device comprised of a plurality of zones of differing wettability, concentrating the sample in the zone of highest degree of wettability, and wherein the zone with the highest degree of wettabilty substantially binds analytes.
32. The method of claim 30, further comprising detecting the analytes in the sample concentrated in the zone of highest degree of wettability.
33. The method of claim 32, wherein detecting analytes in the sample comprises one of the group consisting of mass spectrometry, surface plasmon resonance, fluorescence, atomic force microscopy, optical spectroscopy, bioluminescence, chemiluminescence, x-ray, photoelectron spectroscopy, ellipsometry, electrochemical detection, phosphorescence, ultraviolet spectroscopy, visible spectroscopy, and infrared spectroscopy.
34. The method of claim 33, wherein the mass spectrometry is laser desorption ionization mass spectrometry.
35. A method of modifying analytes using the sample presentation device of claim 7, comprising modifying the analytes within the liquid retention zone or the analysis zone or both.
36. The method of claim 35, wherein modification of the analytes is reversible.
37. The method of claim 35, wherein modification of the analytes is irreversible.
38. A method of altering the wettability of one or more zones of the sample presentation device of claim 1, comprising modifying the surface of the sample presentation device by physical stimuli or chemical stimuli or both, wherein the relative wettabilities of the zones are altered.
39. The method of claim 38, wherein the modification of the surface of the sample presentation device is reversible.
40. The method of claim 38, wherein the modification of the surface of the sample presentation device is irreversible.
41. A method of positioning one or more samples using the sample presentation device of claim 1, wherein the one or more samples move from a point of initial contact to one or more zones of higher wettability relative to the point of initial contact.
42. A method of positioning one or more samples using the sample presentation device of claim 10, wherein the one or more liquid samples move from a point of initial contact to one or more zones of higher wettability relative to the point of initial contact.
CA2531972A 2003-07-14 2003-07-14 Sample presentation device with differing wettability Expired - Fee Related CA2531972C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2003/021786 WO2005016530A1 (en) 2003-07-14 2003-07-14 Sample presentation device with differing wettability

Publications (2)

Publication Number Publication Date
CA2531972A1 true CA2531972A1 (en) 2005-02-24
CA2531972C CA2531972C (en) 2011-11-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2531972A Expired - Fee Related CA2531972C (en) 2003-07-14 2003-07-14 Sample presentation device with differing wettability

Country Status (6)

Country Link
EP (1) EP1656202A1 (en)
JP (1) JP4668064B2 (en)
CN (1) CN100431707C (en)
AU (1) AU2003304421B2 (en)
CA (1) CA2531972C (en)
WO (1) WO2005016530A1 (en)

Cited By (1)

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WO2020030917A1 (en) * 2018-08-10 2020-02-13 Oxford University Innovation Limited Method and apparatus for providing an isolated single cell

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JP4505287B2 (en) * 2003-08-27 2010-07-21 パナソニック株式会社 Microchip, manufacturing method thereof, and inspection method using the same
JP2008541067A (en) * 2005-05-12 2008-11-20 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング Biological sample carrier for mass spectrometry
US7569071B2 (en) 2005-09-21 2009-08-04 Boston Scientific Scimed, Inc. Venous valve, system, and method with sinus pocket
FR2943785B1 (en) * 2009-03-31 2012-11-30 Centre Nat Rech Scient METHOD FOR DETECTING AND QUANTIFYING INTEREST ANALYTES IN A LIQUID AND DEVICE FOR CARRYING OUT SAID METHOD
AU2013231955A1 (en) * 2012-03-16 2014-10-30 Life Technologies Corporation Systems and methods for loading liquid samples
US10921295B2 (en) * 2017-09-08 2021-02-16 Elemental Scientific, Inc. Automated system for detection of silicon species in phosphoric acid
WO2020159088A1 (en) * 2019-01-29 2020-08-06 한국표준과학연구원 Method for measuring organic matter in blood by using ldi-ms, and device therefor

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020030917A1 (en) * 2018-08-10 2020-02-13 Oxford University Innovation Limited Method and apparatus for providing an isolated single cell
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Also Published As

Publication number Publication date
CA2531972C (en) 2011-11-29
WO2005016530A1 (en) 2005-02-24
CN100431707C (en) 2008-11-12
JP2007521458A (en) 2007-08-02
AU2003304421B2 (en) 2009-12-03
EP1656202A1 (en) 2006-05-17
JP4668064B2 (en) 2011-04-13
AU2003304421A1 (en) 2005-03-07
CN1863599A (en) 2006-11-15

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Effective date: 20160714