CA2565125A1 - Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins - Google Patents

Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Download PDF

Info

Publication number
CA2565125A1
CA2565125A1 CA002565125A CA2565125A CA2565125A1 CA 2565125 A1 CA2565125 A1 CA 2565125A1 CA 002565125 A CA002565125 A CA 002565125A CA 2565125 A CA2565125 A CA 2565125A CA 2565125 A1 CA2565125 A1 CA 2565125A1
Authority
CA
Canada
Prior art keywords
seq
nucleic acid
acid sequence
pichia
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002565125A
Other languages
English (en)
French (fr)
Inventor
Piotr Bobrowicz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlycoFi Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2565125A1 publication Critical patent/CA2565125A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002565125A 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins Abandoned CA2565125A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US56673604P 2004-04-29 2004-04-29
US62018604P 2004-10-18 2004-10-18
US60/620,186 2004-10-18
PCT/IB2005/051415 WO2005106010A2 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Publications (1)

Publication Number Publication Date
CA2565125A1 true CA2565125A1 (en) 2005-11-10

Family

ID=40588462

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002565125A Abandoned CA2565125A1 (en) 2004-04-29 2005-04-29 Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins

Country Status (7)

Country Link
US (3) US7465577B2 (cg-RX-API-DMAC7.html)
EP (1) EP1747280B1 (cg-RX-API-DMAC7.html)
JP (1) JP4954866B2 (cg-RX-API-DMAC7.html)
CN (1) CN101084233B (cg-RX-API-DMAC7.html)
AU (1) AU2005238308B8 (cg-RX-API-DMAC7.html)
CA (1) CA2565125A1 (cg-RX-API-DMAC7.html)
WO (1) WO2005106010A2 (cg-RX-API-DMAC7.html)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060024304A1 (en) * 2000-06-28 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Man5GlcNAc2 glycoform
US7795002B2 (en) * 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US20060029604A1 (en) * 2000-06-28 2006-02-09 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAc2Man3GlcNAc2 glycoform
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US20060034828A1 (en) * 2000-06-28 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a GlcNAcMAN5GLCNAC2 glycoform
CA2471551C (en) * 2001-12-27 2014-09-30 Glycofi, Inc. Methods to engineer mammalian-type carbohydrate structures
US20060024292A1 (en) * 2001-12-27 2006-02-02 Gerngross Tillman U Immunoglobulins comprising predominantly a Gal2GlcNAc2Man3GlcNAc2 glycoform
US20060034829A1 (en) * 2001-12-27 2006-02-16 Gerngross Tillman U Immunoglobulins comprising predominantly a MAN3GLCNAC2 glycoform
US6964784B2 (en) * 2002-03-07 2005-11-15 Optigenex, Inc. Method of preparation and composition of a water soluble extract of the bioactive component of the plant species uncaria for enhancing immune, anti-inflammatory, anti-tumor and dna repair processes of warm blooded animals
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
CA2558635A1 (en) * 2004-03-17 2005-09-29 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
JP5752582B2 (ja) * 2004-04-29 2015-07-22 グライコフィ， インコーポレイテッド 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法
EP1942935A4 (en) * 2005-09-02 2009-12-23 Glycofi Inc IMMUNOGLOBULINS CONTAINING PRESENTLY A GLCNACMAN3GLCNAC2 GLYCOFORM
AU2005336263A1 (en) * 2005-09-09 2007-03-15 Glycofi, Inc. Immunoglobulin comprising predominantly a man7GlcNAc2, Man8GlcNAc2 glycoform
EP1954815B1 (en) * 2005-11-15 2015-02-25 GlycoFi, Inc. Production of glycoproteins with reduced o-glycosylation
EP2022855B1 (en) * 2006-05-16 2015-08-19 Daiichi Sankyo Company, Limited High secretion production method of protein
CA2651456A1 (en) * 2006-05-19 2007-11-29 Glycofi, Inc. Recombinant vectors
KR20090130029A (ko) * 2007-03-07 2009-12-17 글리코파이, 인크. 푸코실화가 변형된 당단백질의 제조
JP5967861B2 (ja) 2007-04-03 2016-08-10 オキシレイン ユーケー リミテッド 分子のグリコシル化
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
WO2009089154A2 (en) * 2008-01-03 2009-07-16 Cornell Research Foundation, Inc. Glycosylated protein expression in prokaryotes
JP2011514157A (ja) 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
MX2010013059A (es) 2008-05-30 2010-12-20 Glycofi Inc Cepa de levadura para la produccion de proteinas con galactosa alfa-1,3-enlazada terminal.
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
WO2010019487A1 (en) 2008-08-12 2010-02-18 Glycofi, Inc. Improved vectors and yeast strains for protein production: ca2+ atpase overexpression
EP2401378B1 (en) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast pichia pastoris
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
JP2013507921A (ja) * 2009-10-16 2013-03-07 メルク・シャープ・エンド・ドーム・コーポレイション 宿主細胞抗原に対する抗体に対する検出可能な交差結合活性を欠くタンパク質の、ピチア・パストリスにおける製造方法
JP2013509180A (ja) 2009-10-30 2013-03-14 メルク・シャープ・エンド・ドーム・コーポレイション 改善された分泌効率を有する組換えタンパク質の産生方法
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
US20130053550A1 (en) 2009-11-19 2013-02-28 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
BR112012020882A2 (pt) 2010-02-24 2015-11-03 Merck Sharp & Dohme métodos para produzir uma glicoproteína heteróloga e para produzir uma composição de glicoproteína, célula hospedeira, composição de glicoproteína, e, uso da pichia pastoris recombinante.
AU2011258425B2 (en) 2010-05-27 2015-12-10 Merck Sharp & Dohme Llc Method for preparing antibodies having improved properties
JP6131190B2 (ja) 2010-09-29 2017-05-17 オキシレイン ユーケー リミテッド リン酸化ｎ−グリカンの脱マンノシル化
BR112013007616B1 (pt) 2010-09-29 2023-01-24 Oxyrane Uk Limited Métodos para retirar o cap ("uncapping") de porções de manose-1- fosfo-6-manose e desmanosilar n-glicanos fosforilados em uma glicoproteína, para direcionar uma glicoproteína para o interior de uma célula de mamífero, e para converter uma glicoproteína de uma primeira forma que não se liga substancialmente a um receptor de manose-6-fosfato em uma segunda forma que se liga substancialmente a um receptor de manose-6-fosfato em uma célula de mamífero
US9365846B2 (en) 2010-12-01 2016-06-14 Merck Sharp & Dohme Corp. Surface, anchored Fc-bait antibody display system
WO2012115903A2 (en) 2011-02-25 2012-08-30 Merck Sharp & Dohme Corp. Yeast strain for the production of proteins with modified o-glycosylation
WO2012145666A1 (en) * 2011-04-21 2012-10-26 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Peptide and conjugate vaccines for fungal infections
AU2012258907A1 (en) 2011-05-25 2013-11-07 Merck Sharp & Dohme Corp. Method for preparing Fc-containing polypeptides having improved properties
WO2013062940A1 (en) 2011-10-28 2013-05-02 Merck Sharp & Dohme Corp. Engineered lower eukaryotic host strains for recombinant protein expression
CA2867237C (en) 2012-03-15 2024-01-02 Wouter Vervecken Methods and materials for treatment of pompe's disease
CN104271816A (zh) 2012-05-11 2015-01-07 默沙东公司 表面锚定的轻链诱饵抗体展示系统
DK2852610T3 (en) 2012-05-23 2018-09-03 Glykos Finland Oy PRODUCTION OF FUCOSYLED GLYCOPROTEIN
AU2013335065B2 (en) 2012-10-22 2018-10-25 Merck Sharp & Dohme Llc CRZ1 mutant fungal cells
EP2925345B1 (en) 2012-12-03 2018-09-05 Merck Sharp & Dohme Corp. Method for making o-glycosylated carboxy terminal portion (ctp) peptide-based insulin and insulin analogues
AU2013363266A1 (en) * 2012-12-17 2015-05-07 Merck Sharp & Dohme Corp. pmt2, och1, pmt5 Mutant Cells
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
WO2017117539A1 (en) 2015-12-30 2017-07-06 Northwestern University Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation
CN105671021B (zh) * 2016-03-01 2019-06-28 山东大学 甘露糖苷酶i的表达基因的应用
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020072127A2 (en) 2018-08-03 2020-04-09 Northwestern University On demand, portable, cell-free molecular sensing platform
EP3908669A4 (en) 2019-01-11 2023-05-03 Northwestern University SYNTHESIS OF BIOCONJUGATED VACCINES IN PROKARYOT CELL LYSATE
US12325884B2 (en) 2019-03-04 2025-06-10 Northwestern University Riboswitch-based fluoride sensing in cell-free extract
US12226410B2 (en) 2019-10-18 2025-02-18 Northwestern University Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6
WO2021081485A1 (en) 2019-10-25 2021-04-29 Northwestern University Cell-free extract preparation protocol for enrichment of membrane vesicles and increased glycoprotein yields
CN114645029B (zh) * 2022-03-11 2024-09-10 上海交通大学 分离人工合成的MpgS蛋白质多肽和MpgP蛋白质多肽及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2080804A (en) 1935-04-30 1937-05-18 John E Brantly Well drilling control system
US5272066A (en) * 1986-03-07 1993-12-21 Massachusetts Institute Of Technology Synthetic method for enhancing glycoprotein stability
WO2001088143A1 (en) * 2000-05-17 2001-11-22 Mitsubishi Pharma Corporation Process for producing protein with reduction of acidic sugar chain and glycoprotein produced thereby
US7029872B2 (en) * 2000-06-28 2006-04-18 Glycofi, Inc Methods for producing modified glycoproteins
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
CA2471551C (en) * 2001-12-27 2014-09-30 Glycofi, Inc. Methods to engineer mammalian-type carbohydrate structures
US7507573B2 (en) * 2003-11-14 2009-03-24 Vib, Vzw Modification of protein glycosylation in methylotrophic yeast
EP1696864B1 (en) * 2003-12-24 2014-11-12 GlycoFi, Inc. Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins

Also Published As

Publication number Publication date
WO2005106010A3 (en) 2007-08-16
CN101084233A (zh) 2007-12-05
AU2005238308B2 (en) 2009-05-14
JP4954866B2 (ja) 2012-06-20
US7713719B2 (en) 2010-05-11
US8158384B2 (en) 2012-04-17
CN101084233B (zh) 2012-08-15
US20090117616A1 (en) 2009-05-07
EP1747280B1 (en) 2018-01-17
AU2005238308A1 (en) 2005-11-10
JP2007534330A (ja) 2007-11-29
WO2005106010A2 (en) 2005-11-10
US7465577B2 (en) 2008-12-16
US20090093020A1 (en) 2009-04-09
US20060211085A1 (en) 2006-09-21
EP1747280A2 (en) 2007-01-31
AU2005238308B8 (en) 2009-10-08
EP1747280A4 (en) 2011-11-09

Similar Documents

Publication Publication Date Title
AU2005238308B8 (en) Methods for reducing or eliminating alpha-mannosidase resistant glycans in the production of glycoproteins
JP2007534330A5 (cg-RX-API-DMAC7.html)
EP1696864B1 (en) Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
EP1505149B1 (en) Methylotroph yeast producing mammalian type sugar chain
EP1283265B1 (en) Process for producing protein with reduction of mannose phosphate in the sugar chain and glycoprotein produced thereby
JP2012506710A (ja) 宿主細胞中での糖鎖付加タンパク質の産生のための新規ツール
KR20140091018A (ko) 재조합 단백질 발현을 위한 조작된 하등 진핵 숙주 균주
US10100343B2 (en) CRZ1 mutant fungal cells
EP2678440B1 (en) Yeast strain for the production of proteins with modified o-glycosylation
US20150337274A1 (en) Pmt2-, och1-, pmt5- mutant cells
JP5752582B2 (ja) 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20170116