CA2560945C - Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity - Google Patents

Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity Download PDF

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Publication number
CA2560945C
CA2560945C CA2560945A CA2560945A CA2560945C CA 2560945 C CA2560945 C CA 2560945C CA 2560945 A CA2560945 A CA 2560945A CA 2560945 A CA2560945 A CA 2560945A CA 2560945 C CA2560945 C CA 2560945C
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Canada
Prior art keywords
polymerase
probe
nucleic acid
glu
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CA2560945A
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English (en)
French (fr)
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CA2560945A1 (en
Inventor
Bin Li
Lei Xi
Yan Wang
Peter B. Vander Horn
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Bio Rad Laboratories Inc
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Bio Rad Laboratories Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2560945A 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity Expired - Lifetime CA2560945C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US55913704P 2004-04-01 2004-04-01
US60/559,137 2004-04-01
PCT/US2005/010782 WO2005098042A2 (en) 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity

Publications (2)

Publication Number Publication Date
CA2560945A1 CA2560945A1 (en) 2005-10-20
CA2560945C true CA2560945C (en) 2013-06-18

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ID=35125684

Family Applications (1)

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CA2560945A Expired - Lifetime CA2560945C (en) 2004-04-01 2005-03-31 Quantitative amplification with a labeled probe and 3' to 5' exonuclease activity

Country Status (6)

Country Link
US (2) US7445898B2 (https=)
EP (1) EP1740711A2 (https=)
JP (1) JP4972541B2 (https=)
AU (1) AU2005230833B2 (https=)
CA (1) CA2560945C (https=)
WO (1) WO2005098042A2 (https=)

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CN104039978A (zh) * 2011-09-29 2014-09-10 露美内克丝公司 水解探针
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WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
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CN110325639A (zh) * 2017-02-22 2019-10-11 株式会社友华 具备假阳性抑制功能的探针、其设计方法及其应用
CN112424377A (zh) 2018-04-17 2021-02-26 克罗玛科德公司 用于多重分析的方法和系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP2022513739A (ja) 2018-12-14 2022-02-09 アルティヴュー， インク． 標的を逐次検出するための方法及び組成物
CN110724729A (zh) * 2019-11-18 2020-01-24 中国科学院成都生物研究所 一种荧光探针
US20240025939A1 (en) * 2020-03-06 2024-01-25 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN111518873B (zh) * 2020-05-11 2024-07-09 上海羿鸣生物科技有限公司 一种优化的扩增目标核酸的方法及应用
US20210381067A1 (en) * 2020-06-08 2021-12-09 Ampliwise Inc. Nucleic acid amplification and detection with attenuaiting probe
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Also Published As

Publication number Publication date
CA2560945A1 (en) 2005-10-20
WO2005098042A2 (en) 2005-10-20
US20100159447A1 (en) 2010-06-24
EP1740711A2 (en) 2007-01-10
AU2005230833A1 (en) 2005-10-20
AU2005230833B2 (en) 2011-04-14
WO2005098042A3 (en) 2006-08-24
US8426132B2 (en) 2013-04-23
JP4972541B2 (ja) 2012-07-11
US7445898B2 (en) 2008-11-04
US20060024695A1 (en) 2006-02-02
JP2007531527A (ja) 2007-11-08

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