CA2556997A1 - Procedes de diversification genetique dans la conversion genetique de cellules actives - Google Patents

Procedes de diversification genetique dans la conversion genetique de cellules actives Download PDF

Info

Publication number
CA2556997A1
CA2556997A1 CA002556997A CA2556997A CA2556997A1 CA 2556997 A1 CA2556997 A1 CA 2556997A1 CA 002556997 A CA002556997 A CA 002556997A CA 2556997 A CA2556997 A CA 2556997A CA 2556997 A1 CA2556997 A1 CA 2556997A1
Authority
CA
Canada
Prior art keywords
cell
nucleic acid
gene
target nucleic
hypermutation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002556997A
Other languages
English (en)
Inventor
Jean-Marie Buerstedde
Hiroshi Arakawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum Muenchen Deutsches Forschungszentrum fuer Gesundheit und Umwelt GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2556997A1 publication Critical patent/CA2556997A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA002556997A 2004-02-23 2005-02-23 Procedes de diversification genetique dans la conversion genetique de cellules actives Abandoned CA2556997A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04004062.8 2004-02-23
EP04004062A EP1568765A1 (fr) 2004-02-23 2004-02-23 Méthode de diversité génétique dans des cellules capables de conversion génique
PCT/EP2005/001897 WO2005080552A1 (fr) 2004-02-23 2005-02-23 Procedes de diversification genetique dans la conversion genetique de cellules actives

Publications (1)

Publication Number Publication Date
CA2556997A1 true CA2556997A1 (fr) 2005-09-01

Family

ID=34745862

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002556997A Abandoned CA2556997A1 (fr) 2004-02-23 2005-02-23 Procedes de diversification genetique dans la conversion genetique de cellules actives

Country Status (11)

Country Link
US (2) US8518699B2 (fr)
EP (2) EP1568765A1 (fr)
JP (2) JP5192153B2 (fr)
CN (2) CN103396997A (fr)
AT (1) ATE376584T1 (fr)
AU (1) AU2005215914C1 (fr)
CA (1) CA2556997A1 (fr)
DE (1) DE602005003011T2 (fr)
DK (1) DK1718734T3 (fr)
ES (1) ES2297672T3 (fr)
WO (1) WO2005080552A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008103474A1 (fr) * 2007-02-20 2008-08-28 Anaptysbio, Inc. Procédés de production de pharmacothèques, et leurs utilisations
CA2685714C (fr) 2007-05-31 2016-04-12 University Of Washington Mutagenese inductible de genes cibles
EP2108696A1 (fr) * 2008-04-10 2009-10-14 Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt GmbH Activateur de diversification cis-active et procédé de diversification sélective d'acide nucléiques
CA2865506C (fr) 2012-03-15 2021-07-06 Omeros Corporation Composition et procede de diversification de sequences cible
US11608570B2 (en) 2016-07-29 2023-03-21 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Targeted in situ protein diversification by site directed DNA cleavage and repair
CN113436679B (zh) * 2020-03-23 2024-05-10 北京合生基因科技有限公司 确定待测核酸样本变异率的方法和系统

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7122339B2 (en) * 1998-10-09 2006-10-17 Medical Research Council Method for generating diversity
PT1117774E (pt) * 1998-10-09 2010-02-17 Medical Res Council Método para produção de diversidade
CA2471392A1 (fr) * 2001-12-22 2003-08-21 4-Antibody Ag Methode de generation de lymphocytes precurseurs de vertebres genetiquement modifies et leur utilisation dans la production de proteines de liaison heterologues

Also Published As

Publication number Publication date
US8518699B2 (en) 2013-08-27
ES2297672T3 (es) 2008-05-01
US20070186292A1 (en) 2007-08-09
JP2012210229A (ja) 2012-11-01
WO2005080552A1 (fr) 2005-09-01
DE602005003011T2 (de) 2008-08-14
US20140068795A1 (en) 2014-03-06
CN103396997A (zh) 2013-11-20
AU2005215914C1 (en) 2010-12-09
EP1718734A1 (fr) 2006-11-08
ATE376584T1 (de) 2007-11-15
DE602005003011D1 (de) 2007-12-06
JP5192153B2 (ja) 2013-05-08
CN1934244A (zh) 2007-03-21
EP1718734B8 (fr) 2008-05-21
AU2005215914B2 (en) 2010-06-10
EP1568765A1 (fr) 2005-08-31
JP2007522813A (ja) 2007-08-16
DK1718734T3 (da) 2008-02-25
EP1718734B1 (fr) 2007-10-24
AU2005215914A1 (en) 2005-09-01

Similar Documents

Publication Publication Date Title
Arakawa et al. Activation-induced cytidine deaminase initiates immunoglobulin gene conversion and hypermutation by a common intermediate
US20140068795A1 (en) Methods for Genetic Diversification in Gene Conversion Active Cells
US20080193979A1 (en) Method for generating diversity
Yang et al. Control of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences
EP2204447B1 (fr) Méthode de génération de diversité
AU2002310621A1 (en) Method for generating diversity
JP2004529613A (ja) 改善された抗体特性を有する遺伝的に改変された抗体産生細胞系統を作製するための方法
Komori et al. Biased dA/dT somatic hypermutation as regulated by the heavy chain intronic iEμ enhancer and 3′ Eα enhancers in human lymphoblastoid B cells
EP2641967B1 (fr) Procédé pour la production d' une cellule-b qui produit des anticorps de type humain
US20020155453A1 (en) Method for generating diversity
Taccioli Molecular genetics of antibody diversity
JP2010042017A (ja) 多様性の生成方法

Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20150224